Next Generation Sequencing (NGS)...
27
Genomics and the use of Next Generation Sequencing (NGS) Techniques
Transcript of Next Generation Sequencing (NGS)...
Genomics and the use of Next Generation
Sequencing (NGS) Techniques
Genomics
The study of the structure organization function evolution of genomes (including interactions with the environment)
Genomics before NGS
1900 1977
1980 1990
1994-98
1998
2000
Watson and Crick
identify DNA
(the double helix) as
the Chemical basis of
heredity
Human Genome
Projects (HPG)
begins-an
international effort
to map and
sequence all the
genes in the
human genome
Sanger and Gilbert
derive methods of
sequencing DNA
Working Draft of the
human genome
sequencing complete
Human
genome
project
completed
2003
Rediscovery of
Mendels laws helps
establish the science
of genetics
Human genome project
Cost for sequencing genomes rapidly reduced
What is NGS
NGS platforms
Illumina454
ABI SOLiD
Illumina technologies
Making Libraries
Cluster generation
Sequencing
Data Analysis
Paired-End Sequencing
Multiplexing- barcoding
Alignment and Data analysis
Scope of Sequencing
Diversity studies
Expression studies
The developmental transcriptome of Drosophila melanogaster
Sequence gemone of single cell
Dissecting biological ldquodark matterrdquo with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth PNAS 104 (2007)
Forward genetics- Isolating Mutant genes
Obholzer N et al Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing Development 139 4280ndash4290 (2012)
Reverse genetics- TILLING
A diploid wheat TILLING resource for wheat functional genomics
BMC Plant Biology 2012 12205
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Genomics
The study of the structure organization function evolution of genomes (including interactions with the environment)
Genomics before NGS
1900 1977
1980 1990
1994-98
1998
2000
Watson and Crick
identify DNA
(the double helix) as
the Chemical basis of
heredity
Human Genome
Projects (HPG)
begins-an
international effort
to map and
sequence all the
genes in the
human genome
Sanger and Gilbert
derive methods of
sequencing DNA
Working Draft of the
human genome
sequencing complete
Human
genome
project
completed
2003
Rediscovery of
Mendels laws helps
establish the science
of genetics
Human genome project
Cost for sequencing genomes rapidly reduced
What is NGS
NGS platforms
Illumina454
ABI SOLiD
Illumina technologies
Making Libraries
Cluster generation
Sequencing
Data Analysis
Paired-End Sequencing
Multiplexing- barcoding
Alignment and Data analysis
Scope of Sequencing
Diversity studies
Expression studies
The developmental transcriptome of Drosophila melanogaster
Sequence gemone of single cell
Dissecting biological ldquodark matterrdquo with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth PNAS 104 (2007)
Forward genetics- Isolating Mutant genes
Obholzer N et al Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing Development 139 4280ndash4290 (2012)
Reverse genetics- TILLING
A diploid wheat TILLING resource for wheat functional genomics
BMC Plant Biology 2012 12205
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Genomics before NGS
1900 1977
1980 1990
1994-98
1998
2000
Watson and Crick
identify DNA
(the double helix) as
the Chemical basis of
heredity
Human Genome
Projects (HPG)
begins-an
international effort
to map and
sequence all the
genes in the
human genome
Sanger and Gilbert
derive methods of
sequencing DNA
Working Draft of the
human genome
sequencing complete
Human
genome
project
completed
2003
Rediscovery of
Mendels laws helps
establish the science
of genetics
Human genome project
Cost for sequencing genomes rapidly reduced
What is NGS
NGS platforms
Illumina454
ABI SOLiD
Illumina technologies
Making Libraries
Cluster generation
Sequencing
Data Analysis
Paired-End Sequencing
Multiplexing- barcoding
Alignment and Data analysis
Scope of Sequencing
Diversity studies
Expression studies
The developmental transcriptome of Drosophila melanogaster
Sequence gemone of single cell
Dissecting biological ldquodark matterrdquo with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth PNAS 104 (2007)
Forward genetics- Isolating Mutant genes
Obholzer N et al Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing Development 139 4280ndash4290 (2012)
Reverse genetics- TILLING
A diploid wheat TILLING resource for wheat functional genomics
BMC Plant Biology 2012 12205
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Human genome project
Cost for sequencing genomes rapidly reduced
What is NGS
NGS platforms
Illumina454
ABI SOLiD
Illumina technologies
Making Libraries
Cluster generation
Sequencing
Data Analysis
Paired-End Sequencing
Multiplexing- barcoding
Alignment and Data analysis
Scope of Sequencing
Diversity studies
Expression studies
The developmental transcriptome of Drosophila melanogaster
Sequence gemone of single cell
Dissecting biological ldquodark matterrdquo with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth PNAS 104 (2007)
Forward genetics- Isolating Mutant genes
Obholzer N et al Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing Development 139 4280ndash4290 (2012)
Reverse genetics- TILLING
A diploid wheat TILLING resource for wheat functional genomics
BMC Plant Biology 2012 12205
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Cost for sequencing genomes rapidly reduced
What is NGS
NGS platforms
Illumina454
ABI SOLiD
Illumina technologies
Making Libraries
Cluster generation
Sequencing
Data Analysis
Paired-End Sequencing
Multiplexing- barcoding
Alignment and Data analysis
Scope of Sequencing
Diversity studies
Expression studies
The developmental transcriptome of Drosophila melanogaster
Sequence gemone of single cell
Dissecting biological ldquodark matterrdquo with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth PNAS 104 (2007)
Forward genetics- Isolating Mutant genes
Obholzer N et al Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing Development 139 4280ndash4290 (2012)
Reverse genetics- TILLING
A diploid wheat TILLING resource for wheat functional genomics
BMC Plant Biology 2012 12205
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
What is NGS
NGS platforms
Illumina454
ABI SOLiD
Illumina technologies
Making Libraries
Cluster generation
Sequencing
Data Analysis
Paired-End Sequencing
Multiplexing- barcoding
Alignment and Data analysis
Scope of Sequencing
Diversity studies
Expression studies
The developmental transcriptome of Drosophila melanogaster
Sequence gemone of single cell
Dissecting biological ldquodark matterrdquo with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth PNAS 104 (2007)
Forward genetics- Isolating Mutant genes
Obholzer N et al Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing Development 139 4280ndash4290 (2012)
Reverse genetics- TILLING
A diploid wheat TILLING resource for wheat functional genomics
BMC Plant Biology 2012 12205
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
NGS platforms
Illumina454
ABI SOLiD
Illumina technologies
Making Libraries
Cluster generation
Sequencing
Data Analysis
Paired-End Sequencing
Multiplexing- barcoding
Alignment and Data analysis
Scope of Sequencing
Diversity studies
Expression studies
The developmental transcriptome of Drosophila melanogaster
Sequence gemone of single cell
Dissecting biological ldquodark matterrdquo with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth PNAS 104 (2007)
Forward genetics- Isolating Mutant genes
Obholzer N et al Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing Development 139 4280ndash4290 (2012)
Reverse genetics- TILLING
A diploid wheat TILLING resource for wheat functional genomics
BMC Plant Biology 2012 12205
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Illumina technologies
Making Libraries
Cluster generation
Sequencing
Data Analysis
Paired-End Sequencing
Multiplexing- barcoding
Alignment and Data analysis
Scope of Sequencing
Diversity studies
Expression studies
The developmental transcriptome of Drosophila melanogaster
Sequence gemone of single cell
Dissecting biological ldquodark matterrdquo with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth PNAS 104 (2007)
Forward genetics- Isolating Mutant genes
Obholzer N et al Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing Development 139 4280ndash4290 (2012)
Reverse genetics- TILLING
A diploid wheat TILLING resource for wheat functional genomics
BMC Plant Biology 2012 12205
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Making Libraries
Cluster generation
Sequencing
Data Analysis
Paired-End Sequencing
Multiplexing- barcoding
Alignment and Data analysis
Scope of Sequencing
Diversity studies
Expression studies
The developmental transcriptome of Drosophila melanogaster
Sequence gemone of single cell
Dissecting biological ldquodark matterrdquo with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth PNAS 104 (2007)
Forward genetics- Isolating Mutant genes
Obholzer N et al Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing Development 139 4280ndash4290 (2012)
Reverse genetics- TILLING
A diploid wheat TILLING resource for wheat functional genomics
BMC Plant Biology 2012 12205
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Paired-End Sequencing
Multiplexing- barcoding
Alignment and Data analysis
Scope of Sequencing
Diversity studies
Expression studies
The developmental transcriptome of Drosophila melanogaster
Sequence gemone of single cell
Dissecting biological ldquodark matterrdquo with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth PNAS 104 (2007)
Forward genetics- Isolating Mutant genes
Obholzer N et al Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing Development 139 4280ndash4290 (2012)
Reverse genetics- TILLING
A diploid wheat TILLING resource for wheat functional genomics
BMC Plant Biology 2012 12205
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Multiplexing- barcoding
Alignment and Data analysis
Scope of Sequencing
Diversity studies
Expression studies
The developmental transcriptome of Drosophila melanogaster
Sequence gemone of single cell
Dissecting biological ldquodark matterrdquo with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth PNAS 104 (2007)
Forward genetics- Isolating Mutant genes
Obholzer N et al Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing Development 139 4280ndash4290 (2012)
Reverse genetics- TILLING
A diploid wheat TILLING resource for wheat functional genomics
BMC Plant Biology 2012 12205
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Alignment and Data analysis
Scope of Sequencing
Diversity studies
Expression studies
The developmental transcriptome of Drosophila melanogaster
Sequence gemone of single cell
Dissecting biological ldquodark matterrdquo with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth PNAS 104 (2007)
Forward genetics- Isolating Mutant genes
Obholzer N et al Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing Development 139 4280ndash4290 (2012)
Reverse genetics- TILLING
A diploid wheat TILLING resource for wheat functional genomics
BMC Plant Biology 2012 12205
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Scope of Sequencing
Diversity studies
Expression studies
The developmental transcriptome of Drosophila melanogaster
Sequence gemone of single cell
Dissecting biological ldquodark matterrdquo with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth PNAS 104 (2007)
Forward genetics- Isolating Mutant genes
Obholzer N et al Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing Development 139 4280ndash4290 (2012)
Reverse genetics- TILLING
A diploid wheat TILLING resource for wheat functional genomics
BMC Plant Biology 2012 12205
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Diversity studies
Expression studies
The developmental transcriptome of Drosophila melanogaster
Sequence gemone of single cell
Dissecting biological ldquodark matterrdquo with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth PNAS 104 (2007)
Forward genetics- Isolating Mutant genes
Obholzer N et al Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing Development 139 4280ndash4290 (2012)
Reverse genetics- TILLING
A diploid wheat TILLING resource for wheat functional genomics
BMC Plant Biology 2012 12205
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Expression studies
The developmental transcriptome of Drosophila melanogaster
Sequence gemone of single cell
Dissecting biological ldquodark matterrdquo with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth PNAS 104 (2007)
Forward genetics- Isolating Mutant genes
Obholzer N et al Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing Development 139 4280ndash4290 (2012)
Reverse genetics- TILLING
A diploid wheat TILLING resource for wheat functional genomics
BMC Plant Biology 2012 12205
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Sequence gemone of single cell
Dissecting biological ldquodark matterrdquo with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth PNAS 104 (2007)
Forward genetics- Isolating Mutant genes
Obholzer N et al Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing Development 139 4280ndash4290 (2012)
Reverse genetics- TILLING
A diploid wheat TILLING resource for wheat functional genomics
BMC Plant Biology 2012 12205
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Forward genetics- Isolating Mutant genes
Obholzer N et al Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing Development 139 4280ndash4290 (2012)
Reverse genetics- TILLING
A diploid wheat TILLING resource for wheat functional genomics
BMC Plant Biology 2012 12205
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Reverse genetics- TILLING
A diploid wheat TILLING resource for wheat functional genomics
BMC Plant Biology 2012 12205
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Metagenomics
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
J Virol 2015 May89(10)5340-9 doi 101128JVI00064-15 Epub 2015 Mar 4
A metagenomics and case-control study to identify viruses associated with bovine respiratory disease
Ng TF1 Kondov NO1 Deng X1 Van Eenennaam A2 Neibergs HL3 Delwart E4
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Sequencing ancient genomes
Miller W et al Sequencing the nuclear genome of the extinct woolly mammoth Nature 456 387ndash390 (2008)
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Biological Question
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Exercise
bull Think about what you work on and how sequencing could play a role in your science
bull Come up with a biological question that sequencing could help answer in your work
bull What information do you want from the data
bull What sequencing approach could you use
Third Generation sequencing
Third Generation sequencing
