Bridging the Gap between Biology and Genomic technologies

ISO 9001:2008 Certified Laboratory

Genotypic

Tech

nology

Re

lea

sed

on 1

st M

arc

h 2

011

Established in 1998 – First Genomics R&D Company in India.

Pioneered Microarrays and Next Generation Sequencing Services.

Well equipped with cutting edge technologies to handle large scale genomics projects.

Our core strength is customization and new application development.

Team Genotypic has 8 PhDs and more than 60 Masters.

Teams: qPCR & Molecular biology lab, Certified Microarray Lab, NGS lab, Array Design informatics, Microarray

Analysis, NGS Analysis.

Follow usLargest NGS and Microarray Group in Linkedin – managed by Genotypic www.linkedin.com/groups?about=&gid=1907871&trk=anet_ug_grppro

Plant breeding & Genetics AND Agri Genomics www.linkedin.com/groups?mostPopular=&gid=3186656

• With 2 GAIIx sequencers we specialize in long reads >140 base paired end short turnaround times

• We specialize in custom arrays and non standard application

◊ Gene Expression Profiling (including non coding RNAs)

◊ Cancer genomics◊ miRNA profiling and small RNAs◊ Plant / AgriGenomics◊ Alternative Splice Variants◊ Bovine / Cattle Genomics◊ ChIP on chip & DNA Methylation◊ Toxicogenomics / Clinical genomics◊ aCGH (Comparative Genomics)◊ Biomarker Discovery / Epigenetics◊ CNV(Copy Number Variations)◊ Bacterial Biosensors◊ SNP Validation Arrays ◊ Drug re-purposing/ Drug target discovery◊ InDELs (Insertions & Deletions)

Microarray Services

High quality RNA / DNAextraction and quality control

Catalog OR Custom microarray slide

High resolution scanning andautomated feature extraction with

guaranteed high quality results

Statistical analysis

Biological analysis

Certified Microarray services withGUARANTEED RESULTS

Have an Idea? Talk to us!We have the right solution and technology to translate your ideas into results! Write to us at genomics@genotypic.co.in

Custom Design Arrays

Any organism, Any sequence, Any application

NO SETUP FEES! NO MINIMUM ORDER!

8 x 15K format8 x 60K format

1 x 244K format1 x 1M format

4x 44K format4x 180K format

2 x 105K format2 x 400K format

Ready to use arrays available for most bacteria, fungi, lower eukaryotes, insects, fish, plants, mammals and many more…www.genotypic.co.in/micro_arrays.html

- New

NGS Technological applications Whole genome sequencing

Targeted Re-sequencing

SNP, Mutation identification

Transcriptome sequencing

Digital gene expression

ChIP sequencing

Methylome sequencing

Small RNA sequencing

Targeted Re-sequencing @ Genotypic Full service available for all organisms Sequence selected genes / genomic regions

in multiple samples combined with DNA Capture using Agilent SureSelect Technology - On-Array DNA Capture and In-Solution (Liquid) Capture

Sample/ Library preparation, Multiplexing Compatible with all NGS platforms.

Next Gen Sequencing Services (NGS)

Popular NGS Themes

Bacterial and other microbial sequencing- De-novo and Re-sequencing

Human Genetics- Disease Inheritance studies

Transcriptome/RNA sequencing

Metagenomics

Agrigenomics

Pathogen detection in host system

ChIP Seq combined with ChIP on chip microarrays. Discover and validate Novel protein binding regions.

Elution of Captured Material

Captured DNA ready for Shearing

List of Genes, Exons, Genomic regions/ Choose from catalog DNA Capture designs

Genomic DNA

Fragmentation (Sonication / Shearing)

Repair ends

Ligate adapters

Hybridization

Enrichment by qPCR

Capture Microarray Baits Insolution

Agilent SureSelect Capture

Power of NGS More than 100 times economical than

Sanger sequencing Extremely High throughput- Sequence 100s

of bacterial, small organism genomes Sequence complete human genome in a

week DNA, RNA Sequencing of organisms with

little or no reference information available Data from NGS studies can be used to

custom design unique microarrays

Targeted Re-sequencing using Agilent SureSelect

At Genotypic we have successfully completed more than 200 NGS projects resulting in > 20 publications.

Next Generation Sequencing has revolutionized Biological Research

Sequence regions that matter…

“Any platform, Any format, Any application”

“Align Assemble Annotate Analyze”

Reference Sequence (Optional)

QC Report of reads and reference sequences (Min/Max/Median Read Length, No. & % of HQ Reads, No.

& % of HQ Bases, No. & % of NON ATGC Bases)

Alignment Statistics(% Coverage, % of mapped and unmapped reads, Avg. Read

Depth, No. & Length of Gaps, Avg. Gap Length)

QC on Reads QC on Reference

De Novo Genome assembly

Sequence Data (Reads)

Variation Analysis

Small RNA Assembly

ChIP seq Analysis

Transcriptome Analysis

Genotypic Technology provides the complete microarray data analysis services for Gene expression data generated using any platform. The end-point of analysis includes Biological interpretation of differentially regulated genes into significant functional categories and pathways. Our Reports Include publication quality charts and graphs.

PRELIMINARY DATA ANALYSIS SERVICE

Start Point:Raw Data (.txt, .cel files accepted)

Deliverables :QC reports, List of differentially regulated genes/ Enriched probes with detailed annotation. Significant Gene ontology categories with p-value, Significant pathways regulated with p-value.

Time taken – 3 Days

CUSTOM DATA ANALYSIS SERVICE

Start Point :RawData (.txt, .cel files accepted)Deliverables: Image QC, Raw Data QC, Raw Data,Differentials / Enriched probes with detailed annotation, Significant Gene ontology categories with p-value, Significant pathways regulated withp-value, spreadsheet with gene vs client requested key words with publication evidences.Time taken – 5 Days

BIOLOGICAL ANALYSIS SERVICE

Start Point :Gene list & Keywords

Deliverables: Literature curated gene

Vs keyword relationship.

Time taken – 5 Days

QC, UNDERSTAND, ANALYSE, INTERPERT, APPLY

MICROARRAY DATA ANALYSISCOMPREHENSIVE NGS DATA ANALYSIS

Simple Desktop Application for Rapid Quality Control and Reporting of NGS Data and Processed Sequence Files

www.genotypic.co.in/SeqQC.html

Genotypic’s clientele, collaborators include Indian Institute of Science, UNILEVER, Astra Zenca, Evogene, JNU, IMTECH, Biocon, Wiezmann Institue, Quark Pharma, Novartis, Harvard University…

Genotypic Technology Pvt. Ltd. #259, Apurva, RMV 2nd Stage, 80 Feet Road, Bangalore – 560094, India

Ph: +91-80-40538200 Fax: +91-80-40538222,USA Phone: +1-(215) 253 4495Website: www.genotypic.co.in, E-mail: genomics@genotypic.co.in

www.genotypic.co.in/products.htmlTools for interpretation of Data

Efficient Analysis of High Throughput Genomics Data

NGS – Microarray Lab / Analysis Training / Workshops

www.nextgenseq.com/register