NeoBio™, a natural solution to the challenges

of wastewater treatment

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NeoBio™ Oil & Grease

NeoBio™ Septic

made in

France

wastewater treatment is a public health issue

economic growth generates water pollution

growing population means soaring demand for drinking water: reserves must be protected

treating wastewater = fighting diseases: children, elderly are most impacted

global warming means sanitation, pollution, hygiene problems increase rapidly

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Neo Bio™

what is NeoBio™?

eco-friendly wastewater treatment, made in France

20 natural microorganisms working as an eco-system

developed in collaboration with Louis Pasteur Institute

Class 1: harmless for fish, plants, animals and humans

packaged in water-soluble solid powder 25 gram bags for easy and safe application, convenient logistics

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Septic tanks, portable toilets, RVs, boats, …

key applications

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Grease traps, oil & grease tanks, …

Fish & shrimp farms, ponds, lakes, …

Individual and collective pit latrines

Livestock farms; dairy, meat and other food processing plants

Emergency relief, disaster recovery, …

Neo Bio™

main benefits

eliminates smell in 4 to 6 hours

inhibits pathogenic bacteria: improves public health

stops pollution: reduces BOD, TKN, oil and grease, …

reduces sludge volume by average 40%

cuts costs: no clogs, reduces maintenance intervals

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Neo Bio™

bad bacteria inhibited

gram-positive bacteria gram-negative bacteria mold

Clostridium perfringens Acinetobacter spp. Aspergillus ochraceus

Clostridium septicum Escherichia coli spp. Fusarium moniliforme

Enterococcus faecium Pseudomonas aeruginosa Fusarium proliferatum

Listeria innocua Salmonella typhimurim Penicilium cyclopium

Listeria monocytogenes Salmonella enteritidis Trichoderma harzianum

Micrococcus luteus Salmonella bredeney

Staphylococcus aureus Salmonella derby

Staphylococcus epidermidis Salmonella give

Salmonella indiana

Salmonella infantis

Salmonella kottbus

Salmonella saint paul

Salmonella sandiego

Salmonella virchow

Development of over 25 pathogenic bacteria is limited by production of natural inhibiting substances during NeoBio’s lifecycle.

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Neo Bio™

diseases prevented

NeoBio improves Public Health by inhibiting pathogenic bacteria and reducing nutriments for major disease vectors: mosquitoes, rats, flies, cockroaches, …

NeoBio directly helps fighting:

Dengue Fever

Malaria

Cholera

Diarrhea

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Neo Bio™

additional benefits

reduces need for chemicals: deodorizers, clog removers, …

boosts wastewater treatment plant efficiency

improves methane output for biogas plants

treated sludge becomes bio-fertilizer for agriculture

decreases number of disease vectors: rats, flies, mosquitoes, …

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Neo Bio™

natural microorganisms

Natural = have not been artificially altered.

NeoBio’s microorganisms are registered at the National Collection of Microorganism Cultures of Louis Pasteur Institute, Paris, France, as per the Budapest Treaty.

Class I “harmless” as per European Classification of Microorganisms (European Directive 2000/54/CE, September 18th, 2000)

QPS (Qualified Presumption of Safety) status as per European Food Safety Authority (EFSA-Q-2005-293, November 19th, 2007)

Certified harmless by Thai Department of Agriculture and FDA

Main microorganism strains:

Bacillus subtilis group • Bacillus subtilis • Bacillus amyloliquefaciens

Lactobacillus group • Lactobacillus rhamnosus • Lactococcus lactis ssp. Lactis • Pediococcus pentosaceus

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Neo Bio™

microorganism life cycle

application

24 hours

no smell

48 hours

rats, insects go away

week 1

sludge volume reduced

week 2: stabilization

standard: 1x25gr sachet per water tank cubic meter

smell noticeably drops in 4 to 6h, almost

disappears after 24h

rats, flies, mosquitoes and other disease

vectors stop coming

sludge begins to be liquefied, volume is reduced

by 40% on average

no more smell, pollutants eliminated, pathogenic agents reduced, sludge volume down

r e n e w e d a p p l i c a t i o n m a i n t a i n s b e n e f i t s

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Neo Bio™

comprehensive range

NeoBio™ Septic: for septic tanks, treatment plants, …

NeoBio™ All-Water: for ponds, fish & shrimp farms, …

NeoBio™ Oil & Grease: for grease traps, food industry, …

NeoBio™ Latrines: for pit latrines, livestock farms, …

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Neo Bio™

drop bags directly into tank or influents

use every 2 weeks only, on average

safe to handle with bare hands

shelf life >2 years

no special storage requirements

hassle-free usage

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Neo Bio™

municipal application overview: process flowchart

a p p l i c a t i o n c o l l e c t i o n t r e a t m e n t d i s c h a r g e

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Neo Bio™

Neo Bio™

municipal application overview: benefits at application

quick action: smell stopped, pathogenic bacteria eliminated, water pollution reduced

sludge volume drops by average 40%: holding tanks can be emptied less often

water-soluble solid powder bags: logistics and application made easy

application once every two weeks only

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Neo Bio™

municipal application overview: benefits at collection

no clogs: NeoBio digests toilet paper & organic residue on pipe walls

leaks from ruptured sewer pipes will not pollute soil or groundwater

sludge easier to pump out since it stays semi-liquid

NeoBio can be applied into trucks, collection points

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Neo Bio™

municipal application overview: benefits at treatment plant & discharge

treatment plant capacity boosted: incoming wastewater is pre-treated, lower BOD/TKN/FOG, lower sludge volume, solids & liquids separated, …

no negative impact on plant’s operations

safe discharge into oxidation ponds and environment (including accidental and unauthorized discharges)

NeoBio’s digestive action continues for 2 weeks

benefits are incremental with time

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Neo Bio™

selected references

United Nations UNICEF

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Neo Bio™

selected business cases

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Neo Bio™

Mar. 2005: milk processing plant aerated lagoons, France.

Before: high pollution, thick foam,

surrounding vegetation cannot grow. With NeoBio: pollution stopped, foam

eliminated, lagoon is nuisance-free.

Aug. 2009: Septic tanks at Soneva Kiri Resort by Six Senses, Koh Kood, Thailand

selected business cases

Before: natural stream polluted by

output from worker camp toilets.

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Neo Bio™

NeoBio Septic

used in toilets With NeoBio: pollution is stopped.

selected business cases

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Neo Bio™

Before: grease traps smelly, difficult to

clean, emptied often.

NeoBio used

in sink

With NeoBio, no smell, less

maintenance.

Jan. 2011: grease traps in 7-Eleven stores, Bangkok, Thailand

selected business cases

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Neo Bio™

Before: sludge accumulation, non-

compliance with effluent standards.

NeoBio used

in all tanks

With NeoBio, effluents are compliant

with standards, less maintenance needed

Jun. 2011: municipal wastewater treatment plant, Kuala Lumpur, Malaysia.

Up to 56.8% sludge volume reduction, result of 4 week long comparative test conducted by NGO Solidarités International in March and April 2010 on displaced people camp latrines in Thailand.

Sludge volume reduction ratio

with 25 gram per cubic meter

Sludge volume reduction ratio

with 50 gram per cubic meter Final sludge volume reduction ranking:

NeoBio outperforms competing products

Data provided by NGO Solidarités International, Maesot Camp, Thailand.

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selected business cases Neo Bio™

October 2010, Koh Kood, Thailand: algae infested clarification lagoon in Soneva Kiri by Six Senses Resorts & Spas, Koh Kood, Thailand, depolluted with NeoBio All-Water+

Left: clarification lagoon water before NeoBio.

Right: a few grams of NeoBio All-Water+

were mixed into the water, microorganisms

eliminated algae within days.

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selected business cases Neo Bio™

Head office

Interchange 21 Building, 32nd floor 399 Sukhumvit Road, North Klongtoey, Wattana 10110 Bangkok, Thailand

Office: +66 (0) 2660 3601 Fax: +66 (0) 2660 3881

info@tradinggreenltd.com www.tradinggreenltd.com

contact us

Interchange 21 Building, Bangkok, Thailand.