Natuzyme  A  Mul-  Enzymes  Product  

About  Bioproton  

•  Bioproton  is  an  Australia-­‐  based  biotechnology  

company,  with  specializa:on  in  Feed  Enzymes  

•  Founded  in  1984  in  Finland,  but  in  1993  relocated  to  

Brisbane,  Australia  

•  Currently  exports  feed  enzyme  formula:ons  to  over  

+40countries  

•  Produc:on  and  R&D  facili:es  are  in  Brisbane  

Bioproton  Partners  

•  Australian  Ins:tute  for  Bioengineering  

Nanotechnology  (AIBN),  in  the  Univ.  of  Queensland  

•  AIBN  

–  World  renowned  research  centre    

–   Over  360  researchers  housed  in  a    $75  million  state-­‐of-­‐the-­‐art  

building  complimented  by  an  extensive  suite  of  facili:es.    

•  Bioproton-­‐  Recipient  of  Australian  Government’s  

Australian  Research  Council  Grants  

Bioproton  Quality  Assurance  

Enzymes  in  Animal  Feed  

•  Enzymes  being  added  to  animal  feed  since  1980s  in  response  to  

Economic,  Health  and  Environmental  concerns  

•  Total  enzyme  use  in  animal  feed  is  ~USD600M  with  10%  pa  growth  

•  Phytase  is  the  most  common  enzyme  used  worldwide,  accoun:ng  for  

50%  of  all  sales.    Other  enzymes,  mainly  NSP  Degrading  Enzymes,  

account  for  the  rest.  

Pressure  on  Growers  Today  

What  are  NSPs?  •   NSP-­‐  Non  starch  polysaccharides  

•   Old  name-­‐  Crude  fibers  

•   Cellulose,  Hemicellulose,  Pec:ns  and  Oligosaccharides  

•   Water-­‐  soluble  and  water-­‐  insoluble  NSPs  

•   NSPs  harm  the  bird  indirectly  by-­‐  

a)  Increased  viscosity  of  the  digest,  resul:ng  in  lower  passage  rate  of  nutrients.  

b)  Entrapment  of  Nutrient  (  Cage-­‐effect).      Entrapping  other  nutrients  

mainly  starch,  fat  and  protein  

c)  Influence  on  energy  density  (Dilu:on  effect)  

NSPs  Viscosity increasing polysaccharides

Increased intestinal viscosity

Reduced passage rate of digesta

Reduced feed intake Increased growth of intestinal flora

Nutrient competition between host and microflora

Reduced nutrient intake Increased intestinal viscosity

Reduced nutrient availability

Deterioration of growth performance and feed conversion (FCR)

Increase  Viscosity-­‐  

NSPs  

b)  Encapsula-on  (Cage  effect)-­‐  

  Entrap other nutrients mainly starch, fat and protein

  This is applied to the insoluble parts of the NSP which are present

in the various cell wall structures

NSPs  

c)  Influence  Energy  Density  (Dilu-on  effect)-­‐  

  Poorly digestible

  Dilute the metabolizable energy

  Dilute the nutrient content of feed

NSP  and  Energy  

•  Feed  energy  content  determines  the  price  of  feed.      

•  NSP  content  inversely  propor:onal  energy  content,  so  high  NSP  content  feed  is  cheaper.  

Role of Phytase

Role of Phosphorus •   A  vital  and  expensive  macronutrient  

•   Forma:on  and  maintenance  of  skeletal  structure  (bone  growth  and  calcifica:on)  

•   Metabolic  processes  (energy  release  and  forma:on  of  amino  acids)  

•   3rd  most  expensive  nutrient  in  feed  (aeer  energy  and  protein/amino  acids)  

•   Adequate  dietary  phosphorus  is  required  for  rapid  and  efficient  growth  

•   Availability  of  phosphorus  from  different  dietary  sources  is  variable  

•   Inorganic  phosphorus  –  70  -­‐100%  available  

•   Organic  phosphorus  (phytates)  ~  30%  available  • High  Ca:P  ra:o  can  lower  phosphorus  absorp:on  especially  if  diets  are  marginal  in  phosphorus  

•   Only  18-­‐40%  of  total  phosphorus  consumed  is  actually  digested  

Effects of P Deficiency •  Reduced live weight due to loss of appetite

•  Leg disorders

•  Stiff joints and muscular weakness

•  Increased downgrades due to bone breakage

•  Reduced hatchability

•  Reduced fertility

•  Dysfunction of the ovaries

•  Inhibition, depression and irregularity of Estrus (livestock)

•  Increased vulnerability to disease

P and the Environment •  Only ~ 30% dietary phosphorus is retained by poultry

•  70% excretion to environment

•  If the phosphorus load on the environment exceeds plant uptake then the

excess will be flushed in to lakes and rivers leading to overgrowth of algae

•  Phosphorus is the major pollutant in animal farming areas

•  Strategies that reduce environmental impact of phosphorus from animal production

•  Use of highly digestible sources of dietary phosphorus e.g. MCP/ DCP

•  Phase feeding to more closely match dietary phosphorus to animal needs

•  Use Phytase to improve efficiency of phytate phosphorus utilisation from

feed

Phytate

Phytate is the main storage form of phosphorus in plant seeds

•  Phytates are phytic acid-mineral complexes (salts of phytic acid)

•  Phytate has an important role in physiological functions during dormancy and

germination of seeds

•  Phytate is not uniformly distributed throughout the seed, being most prevalent in:

  Aleurone layer (bran) of wheat and barley

  Germ of corn

  Throughout soybean

About  Natuzyme  

•  Premium  mul:-­‐ac:vity  feed  enzyme  formulated  for  broiler,  layer,  duck,  

milking  cows  and  aqua  feeds.  

•  Natuzyme  contains  cellulase,  xylanase,  beta-­‐glucanase,  alpha-­‐amylase,  

protease,  pec:nase  and  phytase  and  other  enzymes.        

•  Natuzyme   enzymes   have   a   wide   pH   range,   stability   and   temperature  

tolerance  and  high  levels  of  ac:vity.  

•  Natuzyme   has   been   successfully   applied   to   animal   feeds   around   the  

world  with  excellent  results  under  wide  ranging  condi:ons.    

Enzyme  Func-ons  

Broiler  –  Trial  Data  A  •  Trial  Protocol:  To  evaluate  the  effec:veness  of  different  enzyme  products    

–  1.  Natuzyme  

–  2.  Enzyme  R  +  Phytase  

–  3.  Enzyme  A  +  Phytase)    

–  Nega:ve  Control  Diet  vs.  Posi:ve  Control  Diet  over  a  42  day  period.  

•  Posi:ve  Control  (PC)  Diet:  a  typical  corn  /  soybean  meal  used  in  Mid-­‐West  USA.  

•  Nega:ve  Control  (NC)  Diet:  PC  less  140  Kcal/kg  ME  and  less  than  0.1%  Available  

Phosphorous  

•  Trial  Loca:on:  USA,  2010  

•  Results:  Natuzyme  provided  superior  outcome  without  needing  phytase.  

Broiler  –  Trial  Data  A  

Trial  Loca:on:  USA,  2010  

Layer  –  Trial  Data  A  

•  Trial  Protocol:  Natuzyme  was  added  “on-­‐the-­‐top”  and  compared  to  diet  

without  enzymes  in  layers  over  a  30d  period.  

•  Diet:  a  basal  diet  with  combina:on  of  wheat,  barley,  oat,  soybean  and  

sunflower.      

•  Trial  loca:on:  Ukraine  2010  

•  Results:  Natuzyme  group  increased  egg  produc:on  by  3.6  %  and  reduced    

the  number  of  dirty  eggs  from    6.1%  to  2.5%.      

Trial  Loca:on:  Ukraine  2010  

Layer  –  Trial  Data  A  

Layer  –  Trial  Data  B  

•  Trial  Protocol:  350g/Ton  of  Natuzyme  v.  100g/Ton  phytase.  

•  Diet:  a  basal  diet  with  combina:on  of  wheat,  barley,  oat,  

soybean  and  sunflower.      

•  Trial  loca:on:  Russia  2011  

•  Results:  Natuzyme  group  was  beper  in  all  categories  (profit  

margin,  produc:vity,  dirty  eggs,  feed  costs  etc),  resul:ng  

improved  profitability  6.07%.      

Layer  Trial  –  B  Data  

Measure UOM Control  +  500  FTU  Phytase

Control  +  350g/T  Natuzyme

Improvement  with  Natuzyme

Mortality % 2.25% 2.24% -­‐0.44%

Av  Wt  of  Egg gram 63.47 63.48 0.02%

Produc:vity % 92.46% 92.68% 0.24%

Feed  Cost   $US/day/bird 0.0462 0.0455 -­‐1.48%

Value  of  Eggs $US/day/bird 0.0647 0.0649 0.24%

Profit   $US/day/bird 0.0186 0.0194 4.50%

Profit  Margin % 40.2% 42.7% 6.07%

Trial  Loca:on:  Russia  2011  

Compara-ve  Broiler  Trial  A  •  Trial  Protocol:  Compare  FCR  of  leading  NSP  enzyme  products  in  

commercial  feeds  

•  Diets:  Commercial  Wheat  and  Sorghum  diets.  

•  Loca:on:  New  Zealand  2012  

•  Results:  

 1.  Wheat  diets,  Natuzyme  was  FCR  was  0.9%  beper  than  typical  compe:tor  

 2.  Sorghum  diets,  Natuzyme  was  FCR  was  2.44%  beper  than  typical  

Compara-ve  Broiler  Trial  A  

New  Zealand  2012  

Compara-ve  Broiler  Trial  B  

Trial  Loca:on:  Massey  University,  New  Zealand  2010  

Diet   Group   AME,  kJ/kg  DM  Improvement  AME,  kJ/kg  DM  

Improvement,  %  

Wheat  

Control    3,035     -­‐   -­‐  

Average    3,119      84     2.8%  

Natuzyme    3,140      105     3.5%  

Sorghum  

Control    3,525     -­‐   -­‐  

Average    3,556      31     0.9%  

Natuzyme    3,593      68     1.9%  

Swine  -­‐  Trial  Data  B  Diet:      Corn  /  Soybean  Meal  Species:    Weanling  pigs  Loca:on:  Kansas  State  University,  2007  Protocol:  To  evaluate  the  effec:veness  of  Natuzyme  in  Nega:ve  

Control  (NC)  diets  compared  to  Posi:ve  Control  (PC)  in  a  35d  trial  on  weanling  pig  performance.      

PC  Diet:      A  typical  corn  /  soybean  meal  used  in  Mid-­‐West  USA,    NC  Diet:      PC  but  with  12.5%  Soy  Hulls  and  no  an:bio:cs  (165kcal/kg  

less  ME  and  1.1%  less  Protein).  

Results:  Natuzyme  enabled  the  use  of  cheaper  and  lower  quality  feeds  (less  energy  and  protein  and  no  an:bio:cs)  on  a  dose  rate  of  500g/ton.  

Swine  –  Trial  Data  B  

Trial  Loca:on:  Kansas  State  University,  2007          

Milking  Cows  

•  Enzyme  use  in  monogastrics  is  very  established  science.  

•  Ruminants  are  the  next  stage.  

•  Pressure  to  reduce  the  cost  of  producing  milk  and  meat  

•  NDF  (Neutral  Diges:ble  Fibre)  =  30—70%  of  feed.    And  only  ~50%  are  

digested  by  animal.  

•  So  poten:al  is  huge!  

•  Trial  Protocol:  Measure  the  increase  in  milk  produc:on  with  Natuzyme  use  

in  milking  cows.    

•  Diet:  Normal  local  grass  fed  diet  in  Egypt.    Natuzyme  added  at  20g/head/

day  

•  Trial  Loca:on:  Egypt,  2009    

•  Results:  Increases  Milk  Produc:on  (2.43%  and  1.67%)  and  Extend  

Peak  Milk  Produc:on  Period  

Milking  Cow  –  Trial  Data  

Milking  Cow  –  Trial  Data  

Trial  Loca:on:  Egypt  2009,  Natuzyme  added  at  20g/head/day  

Milking  Cow  –  Trial  Data  

•  The  data  in  table  shows  how  Natuzyme  increases  daily  milk  produc:on  and  extends  Peak  Milk  Produc:on  period.    

Source:  Egypt  2009,  Natuzyme  added  at  20g/head/day  

Period   Trial  A     Trial  B    

1  to  20d   +0.09  %  or  +0.03  L/d   +0.16  %  or  +  0.05  L/d  

21  to  41d   +4.57  %  or  +1.29  L/d     +3.04  %  or  +  0.83  L/d  

Average  (1-­‐41d)   +2.43  %  or  +0.67  L/d   +1.67  %  or  +0.45  L/d  

Total  (1-­‐41d)   +  27.7  L   +18.4  L  

Why  Natuzyme?  

•  Simple  -­‐  Single  product,  no  need  to  store  or  use  different  

enzymes  

•  Cost  Effec-ve  –  No  need  to  add  phytase  or  other  enzymes  

•  Flexible  –  Can  be  used  in  all  feed  scenarios,  even  if  not  all  

feed  quali:es  are  not  known.  

•  Synergy  –  e.g.  Phytase  will  work  more  effec:vely  in  

combina:on  with  other  enzymes  “Phytate  availability”  

Natuzyme  Product  Informa-on  

•  Micro-­‐granulated  enzymes  

•  Resistance  to  heat  pelle:ng  up  to  90  deg  C  

•  Storage  for  24mths  at  room  temperature  

•  20kg  Bags  (3  paper  and  1  polyethylene  layers)  

•  Typical  dosage:  

–  Broilers:  350  g/Ton  of  Feed    

–  Layers:    250g/Ton  of  Feed  

–  Milking  Cows:  20g/Head/Day