National Center for Genetic Resources Preservation Fort Collins, CO David Dierig, RL.

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National Center for Genetic Resources Preservation Fort Collins, CO David Dierig, RL

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Transcript of National Center for Genetic Resources Preservation Fort Collins, CO David Dierig, RL.

Page 1: National Center for Genetic Resources Preservation Fort Collins, CO David Dierig, RL.

National Center for Genetic Resources Preservation

Fort Collins, CODavid Dierig, RL

Page 2: National Center for Genetic Resources Preservation Fort Collins, CO David Dierig, RL.

•Research at NCGRP

Page 3: National Center for Genetic Resources Preservation Fort Collins, CO David Dierig, RL.

Non-invasive assays of Seed Longevity and Seed Aging

Christina Walters and Dani Ballesteros

Maize inbred (Pioneer) #1, stored 20 yrs -18oC

25oC

Problem: We cannot tell how long a seed will survive during storage, nor how much it has aged, until we germinate it.

• consume seed • are costly• only describe health at the moment

Germination assays

• have no predictive power

Non-invasive assays are quantitative and predictive

• give categorical (not quantitative) information

6 mm

Volatiles production correlates with longevity

Structural stability correlates with longevity

Lipid crystallization decreases with aging

Volatile Analysis using Gas Chromatography

Dynamic Mechanical Analysis

Differential Scanning Calorimetry

Page 4: National Center for Genetic Resources Preservation Fort Collins, CO David Dierig, RL.

Citrus CryopreservationGayle Volk

Concern: Loss of key collection materials due to Huanglongbing diseaseResponse: Develop strategy to cryopreserve vegetative propagules of Citrus accessions• Receive non-dormant bud material from Riverside• Excise 1 mm shoot tips• Cryopreserve using vitrification• Recovery by micrografting onto seedling rootstock in tissue culture

Grafted, cryopreserved Citrus shoot tips 13 days after micrografting

Customer response: Invitations by Citrus Research Board to present at meetings and to submit proposal for industry funding

Page 5: National Center for Genetic Resources Preservation Fort Collins, CO David Dierig, RL.

We developed analytical methods to estimating adaptive differentiation by integrating • Population genetic variation (SNP, SSR)• Ecological data• Spatial niche modeling analysis

How do we efficiently capture genetic diversity in wild populations?

Chris Richards

Germplasm from wild populations is difficult to preserve because it is heterogeneous, not adapted to storage, and difficult to regenerate.

Representative collections, without duplication, require targeting the diversity “hot spots” or genetic lineages.

N. American HopsWith NCGR Corvallis, OR

Little SunflowerWith NCRPIS Ames, IA

Page 6: National Center for Genetic Resources Preservation Fort Collins, CO David Dierig, RL.

How do traits like seed dormancy or after-ripening affect genetic diversity?

Von Mark Cruz and David Dierig

Traits like after-ripening serve plant populations well in times of extreme environmental conditions to ensure survival but gene flow between plant classes may limit preservation of desired diversity.

Sympatric population of two Lesquerella species in NM

Collections with Gabriella Romano – Parlier, CA

Methods:Phenotypic field measurements and seed collectionsGermination time for plant classes and temperature plus water stress responseMolecular markers to measure diversity Characterization of genes related to seed dormancy

Two plants of the same species at different growth and reproductive maturity stages due to dormancy.

Page 7: National Center for Genetic Resources Preservation Fort Collins, CO David Dierig, RL.

Re-cryoprocessed potato PVP accessions prior to 2004

Maria Jenderek

• Objective: Improve quality of potato accessions in cryopreservation

• Problem: Previous protocols lead to low viability after storage in cryo.

• A new protocol was adapted, and 54 accessions are being reprocessed, thus improving the viability to>50%

• Impact:

• After PVP expires, the potato cultivars will be integrated into the public domain (NPGS)

• The NCGRP is to be reimbursed $2,400/accession for this service

• The value of the U.S. potato industry is >$3 billion

Page 8: National Center for Genetic Resources Preservation Fort Collins, CO David Dierig, RL.

Personnel Issues

Retired tech position filled in FY10 for micrografting, tissue culture with Gayle Volk

New Position is planned to coincide with retirement of Judy Grotenhuis to start in August (Renee White)

New part-time molecular genetics tech Position is being planned with Chris Richards

Post doc started in March with Dave DierigNew HQ post doc to start in Aug with Gayle Volk

Page 9: National Center for Genetic Resources Preservation Fort Collins, CO David Dierig, RL.

Funded SCAs and CRADAs

CSU, CO Seed Lab, $20,000 – Evaluation of seed Longevity of Germplasm Placed in Long Term Storage. Exp. 2010 (plans to renew if possible)

CIMMYT, $38,600 -0 Aid in the Conservation and Regeneration of Maize and Maize Relatives in Mexico. Exp. 2012 (will not fund if no budget increase)

Page 10: National Center for Genetic Resources Preservation Fort Collins, CO David Dierig, RL.

Other Extramural Funds

• New SCA with CSU being developed to collaborate on the Citrus/ Avocado project using HQ funds.

Page 11: National Center for Genetic Resources Preservation Fort Collins, CO David Dierig, RL.

Facility Issues

• We have worked with NREL, ClimateWise, and Lawrence Berkeley Labs on energy audits to reduce utility costs for the facility.

• Roof leaks and age indicate a new roof is needed. We hope to do phase 1 in FY11.

• -18 vault has ice build up issues we’re looking into solutions.

• Phones work on good days.


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National Center for Genetic Resources Preservation Fort Collins, CO David Dierig, RL

National Center for Genetic Resources Preservation Fort Collins, CO David Dierig, RL

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