Nanoporous Membrane Technologies for Pathogen Collection ... · Nanoporous Membrane Technologies...
Transcript of Nanoporous Membrane Technologies for Pathogen Collection ... · Nanoporous Membrane Technologies...
1 of 21
Nanoporous Membrane Technologies for Pathogen Collection, Separation, and
DetectionSang Won Lee, Hao Shang, and Gil U Lee*
School of Chemical Engineering, Forney Hall, Purdue University, West Lafayette, IN 47906
and
Matthew T. Griffin and Jack FultonNVEO & Chem/Bio Sensors Group
NSWC CraneCrane, IN 47522
Distribution Statement A - Approved for public release; distribution unlimited.
Report Documentation Page Form ApprovedOMB No. 0704-0188
Public reporting burden for the collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering andmaintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information,including suggestions for reducing this burden, to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, ArlingtonVA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to a penalty for failing to comply with a collection of information if itdoes not display a currently valid OMB control number.
1. REPORT DATE 19 NOV 2003
2. REPORT TYPE N/A
3. DATES COVERED -
4. TITLE AND SUBTITLE Nanoporous Membrane Technologies for Pathogen Collection,Separation, and Detection
5a. CONTRACT NUMBER
5b. GRANT NUMBER
5c. PROGRAM ELEMENT NUMBER
6. AUTHOR(S) 5d. PROJECT NUMBER
5e. TASK NUMBER
5f. WORK UNIT NUMBER
7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) School of Chemical Engineering, Forney Hall, Purdue University, WestLafayette, IN 47906
8. PERFORMING ORGANIZATIONREPORT NUMBER
9. SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR’S ACRONYM(S)
11. SPONSOR/MONITOR’S REPORT NUMBER(S)
12. DISTRIBUTION/AVAILABILITY STATEMENT Approved for public release, distribution unlimited
13. SUPPLEMENTARY NOTES See also ADM001851, Proceedings of the 2003 Joint Service Scientific Conference on Chemical &Biological Defense Research, 17-20 November 2003. , The original document contains color images.
14. ABSTRACT
15. SUBJECT TERMS
16. SECURITY CLASSIFICATION OF: 17. LIMITATION OF ABSTRACT
UU
18. NUMBEROF PAGES
21
19a. NAME OFRESPONSIBLE PERSON
a. REPORT unclassified
b. ABSTRACT unclassified
c. THIS PAGE unclassified
Standard Form 298 (Rev. 8-98) Prescribed by ANSI Std Z39-18
2 of 21
Approach
Develop membranes and methodology for continuous collection of airborne particles.
Schematic of a Point Detector that Utilizes Membrane
Develop a membranes and and methodology for continuous separation via ultrafiltration.
Develop a receptor functionalized membrane to improve mass transport and kinetic conditions.
3 of 21
Nanoporous Membranes! Nanoporous alumina membranes were chosen as a substrate because of their desirable physical properties and high density of uniform pores of 10-200 nm size.
! The membrane surfaces will be modified with hydrophobic and hydrophilic coatings to facilitate pathogen collection and separation.
! The membrane surfaces will be modified with proteins and nucleic acids to enable pathogen identification. Side view: 100 nm
pore, 100 micron thick alumina membrane
4 of 21
Membrane Chemistries
PEI-PEG (2000)O
H
OH
OH
OH
OH
5 % PEI, pH=8.2
NH
2
NH
2
NH
2
NH
2
NH
2
M-PEG, pH=8.2
OC
H3
OC
H3
OC
H3
OC
H3
OC
H3
OHOHOH
Si
CH3O
CH3O
CH3O OCH3+
OOO
Si OCH3
Reflux in toluene w/ 5%Et3N for 4 hrs under N2
Silane-PEG (5000)
5 of 21
Characterization of Membrane Chemistries
15.3
38.6
11.0
35.8
58.4
% O 1s (530.3 eV)
21.05.650.29.05.0PEI-PEG
35.81.224.5OTMS coating
21.83.446.42.111.0Silane-PEG
12.233.219.2PEI
11.130.3Unmodified
membrane
% O 1s (532.0 eV)
% N 1s (399.1 eV)
% C 1s (286.5 eV)
% C 1s (285.6 eV)
% Si 2p (103.5 eV)
% Al 2p (74.4 eV)
Chemistry
6 of 21
Protein Fouling
1000
800
600
400
200
0
Flu
ore
scen
eIn
ten
sity
(a.
u)
2.01.51.00.50.0
BSA Concentration (mg/ml)
Silane-PEG coated Membrane
Unmodified Membrane
7 of 21
Collector
Air in
To pump Membrane holder Flow meter
Relative humidity meter
HumidityControl
8 of 21
Gas and Liquid Permeabilities
Nitrogen permeability (m/PaS)
Water permeability (m/PaS)
6.84E-06
-
2.03E-06
Silane-PEG
6.88E-6
6.08E-06
1.90E-06
--6.50E-06200 nm
3.14E-064.63E-064.62E-06100 nm
7.08E-082.0E-061.98E-0620 nm
PEGPEIBare membraneHolder 2 Holder 1
2.73E-084.06E-103.04E-092.18E-082.86E-08200 nm
-Foulingn/a2.14E-081.91E-08100 nm
6.59x10-09Fouling1.64E-092.67E-097.00E-0920 nm
Silane-PEGPEGPEIBare membraneHolder 2 Holder 1
9 of 21
Membrane Permeabilities in the Presence of Water
0
100
200
300
400
500
600
700
0 5 10 15 20 25 30
Pressure (PSIG)
Flo
w R
ate
(SC
CM
)
Dry Air
0.028 H2Omolefraction
0.018 H2Omolefraction
0.010 H2Omolefraction
10 of 21
Operation Characteristics
0After filtration
2x106Before
BG spores
(spores/ml)
Collection Efficiency
96.7Sonication in PBS
26.9H2O
76.4SDS 10 %
75.4PBST 0.5 %
Removal Efficiency
(%)
Method of Extraction
Extraction Efficiency
11 of 21
3 Day Laboratory Trial
10x10-6
8
6
4
2
0
Per
mea
bilit
y (m
/Pa.
s)
706050403020100Time (hr.)
12 of 21
Collector Prototype
Air In
PressureSensor
Membrane
Membraneholder
Quick-disconnect
Inlet Cell
Quick-disconnect
Ultrasonic Source
Quick-disconnect In
Out
13 of 21
Performance of the Prototype
0
10
20
30
40
50
60
70
250000.00 350000.00 450000.00 550000.00 650000.00 750000.00 850000.00
Pressure (Pa)
Flo
w R
ate
(lit
res/
min
)
14 of 21
Separator - Solute Permeabilities
20nm membrane
3.5
3.0
2.5
2.0
1.5
1.0
0.5
Ab
s.
12001000800600400200
Time (min)
15 of 21
Protein Permeability
7x10 -708.19x10 -76.37x10-7
Silane-PEGPEI-PEGUnmodified Membrane
Theoretical Permeability of Ovalbumin
(cm2/s)
16 of 21
Collector & Separator Fluidics
17 of 21
Detection
Adsorption Scattering
Patent sensitive
18 of 21
Self-assembled Monolayers
Atomic force microscope image of a 12 nm Au array. Scan size 500 x 500 nm. Z scale 50 nm
19 of 21
Ovalbumin Binding
0.34
0.32
0.30
0.28
0.26
0.24
0.22
0.20
Abs
orba
nce
800700600500400Wavelength (nm)
A)
B)
Patent sensitive
20 of 21
Nanoporous Membrane Separation Methodologies
Point of Contact: Gil Lee
School of Chemical EngineeringPurdue University
West Lafayette, IN 47907-1283765-494-0492
Objective: To fabricate and characterize the performance nanoporous membranes for the collection, separation, and detection of airborne pathogens.
Payoff: A highly sensitive point detector that consumes minimal reagents. Design criteria include < 0.1 ACPLA sensitivity for toxins, viruses and bacteria; < 10 min response time; < 0.5 ml/min total reagent consumption.
Top view:100 nm pore alumina membrane
21 of 21
Acknowledgements
Helpful discussionGavin Reid and Scott McLuckey, Dept. Chemistry, Purdue UniversityNorman Hovijitra, Eric Wallis, and Richardo Chong, School of Chemical EngineeringJoe Brumfield, ONRRichard Haash, UIUCJerry Bottiger, SBBCOM
FundingInstitute for the Detection of Hazardous Materials Purdue University, ONR, and the Shreve Trust.