Nannizziopsiaceaespp. infecng) chelonians) · KF477230_ 0-477232 _ 0-4772.35 _ KF477233_ KF477229_...
Transcript of Nannizziopsiaceaespp. infecng) chelonians) · KF477230_ 0-477232 _ 0-4772.35 _ KF477233_ KF477229_...
Phylogeny es+ma+on of Nannizziopsiaceae spp. infec+ng
chelonians
Dataset
• Fungal Internal Transcribed Spacer (ITS) Region • 57 Sequences Total
• 11 from turtle cases • 45 published sequences from GenBank • 1 outgroup – Coccidioides immi+s
• Sequence length heterogeneity • 418 – 590 bp
Mul+ple Sequence Alignment
• Two MSA methods • MAFFT
• v7.273 • FFN-‐NS-‐2 Strategy
• Clustal Omega • Dealign Input Sequences: No • MBED-‐Like Clustering Guide Tree: Yes • MBED-‐Like Clustering Itera+on: Yes • Number of Combined Itera+ons: default(0) • Max Guide Tree Itera+ons: Default • Max HMM Itera+ons: Default • Order: Aligned
Tree Es+ma+on
• RAxML • v7.7.1 • GTR-‐Gamma model • 100 rapid bootstrap inferences
• Tree comparison • Dendropy4 • 10 FN, 10 FP • Normalized RF Score 0.185185
MAFFT Alignment
Clustal Omega Alignment