Mystery Solution Challenge - Testing for Presence of Starch Sugar and Protein 2
Transcript of Mystery Solution Challenge - Testing for Presence of Starch Sugar and Protein 2
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Mystery Solution
ChallengeWhich solution contains:
GlucoseStarch
Protein?
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Mr Stuart has mixedup 5 importantfood samples (S1-
S5) and needs tofind out which iswhich
You are the fooddetectives that canuse science to help
him
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These are Mr StuartsSamples
2% Egg Albumen
20% Glucose
1% Starch 0.2% Glucose
0.1% Starch
But which is which???
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In front of you there are the 5samples
Take a small amount of one sample andplace in to 3 test tubes so they fill to1/3 full
You need to do all three tests to confirm
which solution is which
Complete the first test to decide if itcontains Starch
Then the second to test for Glucose
The third to determine Protein
presence
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Test for starch
Put a few drops of the mixture fromjar 1 in a test tube. Add a drop ofiodine to the tube.
A positive result turns dark blue
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Test for sugar(glucose)
A couple of drops of Benedicts solnis added to a third of a test tube of
the sample Place the sample into a water bath
and leave for 5 mins
Whilst waiting set up the next test
A positive result turns orange/ brick
red
i b f d
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A Biuret test can be performedin the laboratory to identify the
presence of peptide bonds The tube on the left shows a
negative result.
The tube on the right is a positivetest for protein. Note the purplecolour of the positive test.
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ResultsTest TubeSample
ContentsS1S2S3S4
S5
%2 E g g A lb u m e n
%2 0 G lu co se
%1 S ta rch
. %0 2 G lu co se
. %0 1 S ta rch
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ResultsTest TubeSample
ContentsS1 StarchS2 StarchS3 GlucoseS4 Glucose
S5 Protein
%2 E g g A lb u m e n
%2 0 G lu co se
%1 S ta rch
. %0 2 G lu co se
. %0 1 S ta rch
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http://images.google.co.uk/imgres?imgurl=
http://images.google.co.uk/imgres?imgurl=http://faculty.ntcc.edu/mhearron/BenedictsTest.jpg&imgrefurl=http://faculty.ntcc.edu/mhearron/cell_chemistry.htm&usg=__xtIoaCA4kwlVpNzQ3DeQYCRe4ms=&h=480&w=640&sz=25&hl=en&start=17&sig2=42G7AKRyK_txN29CoYbvqQ&um=1&tbnid=OAHKU806skaMLM:&tbnh=103&tbnw=137&prev=/images?q=testing+for+sugar+benedict%27s&hl=en&safe=active&sa=N&um=1&ei=uWxeS_yDMtaUlAfs74yvAghttp://images.google.co.uk/imgres?imgurl=http://faculty.ntcc.edu/mhearron/BenedictsTest.jpg&imgrefurl=http://faculty.ntcc.edu/mhearron/cell_chemistry.htm&usg=__xtIoaCA4kwlVpNzQ3DeQYCRe4ms=&h=480&w=640&sz=25&hl=en&start=17&sig2=42G7AKRyK_txN29CoYbvqQ&um=1&tbnid=OAHKU806skaMLM:&tbnh=103&tbnw=137&prev=/images?q=testing+for+sugar+benedict%27s&hl=en&safe=active&sa=N&um=1&ei=uWxeS_yDMtaUlAfs74yvAg -
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To make a vit-C indicatorsolution Experiment 1-
Mix 1 tablespoon of cornstarch into enough water to make a paste.
To this paste, add exactly 250 millilitres of water and boil for fiveminutes. Allow to cool.
Add 10 drops of the starch solution to 75 millilitres of water byusing the eyedropper.
Add enough iodine to produce a dark purple-blue color. Now theindicator solution is ready.
Put 5 millilitres of indicator solution (roughly 1 teaspoon) in a 15-milliliter test tube (one for each sample)
To the test tube, use a clean eyedropper to add 10 drops of juicefrom teh fresh fruits. For solids, pulp them in a blender andstrain the juice well. Washand clean the eyedropper for each
sample. Shake well. Place all of the test tubes against a white background.
Line up the tubes from lightest to darkest purple. The lighter thesolution, the higher the level of Vitamin C content. That'sbecause Vitamin C causes the purple indicator solution to loseits color.
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Experiment 2-
Repeat the whole process ofExperiment 1.
Repeat the whole process ofExperiment 1, this time with theconcentrate juices, not the pureones.
Compare the amount of Vitamin C ofthe concentrate juices and thefresh pure ones
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Experiment 3-
Do the whole process of Experiment1, but use Kool-aid, Sunny Delight,and, C-plus instead of the fruit
juices. Compare the results from these
juices with the juices with the juices
from concentrate and the freshpure ones.
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