MS2000 Singapore Training

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    MS2000 Singapore TrainingGeneral

    1) Its instrument which use laser diffraction

    2) Laser type is HeNe Laser.

    3) For large particle, amount of light is higher and angle is smaller.4) For small particle, amount of light get smaller and angle is bigger.

    5) In laser diffraction , it collect the scatter light from different angle to analyze

    through s/w by using MIE theory into PSD ( Particle Size Distribution ) or result.6) Measure size range is 0.02u 2000u for Ms2000. While MS2000E capability

    measurement is only from 0.1u 1000u due to dont have back scatter.

    7) Instrument performance can be verify using latex standard or glass bead ( size

    range is 10u 120u )

    8) MS2000 Background

    a. Electronic noise = dark background which control by laser shuttle

    b. Optical background = all the scattering that we do not want. Such asscattering from :- cell window, lens, cell protection window, liquid/air and

    etc.

    9) Why Not Using Light Bulb??

    a. Not intenseb. Mot monochromatic

    c. Not cohenent

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    No Sample IO app I

    With sample I will decreaseObscuration = ( (IO I )) / IO X 100%

    = ( 1 I/IO) X 100%

    Blue Light

    - 10X signal intensity and its specially use to measure small size particle

    - Improve resolution by making curve- It allow us to see details about the signal.

    DETECTOR

    0 33 = red light obscuration

    34 42 = Side scatter

    43 44 = Large angle red

    45 46 = Large angle blue

    47 48 Back scatter red

    49 50 = Back scatter blue51 = Extinction red52 = extinction blue

    53 = Laser monitor

    54 57 = Alignment

    58 = Blue Light Obsculation

    59 = Offset

    60 = Gain

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    Characterization ( To Perform Backup / Restore On Parameter)

    1) Go to Security Engineering

    2) Tools Engineering Characterization Data

    3) Select the Characterization Type radio button one by one . Choose location for

    the backup , then click UPLOAD.

    4) All together will be 7 files.

    Laser , Spatial Filter , Pin Hole, Main Lens X/Y Alignment

    1) Remove jumper at X/Y Motor board. When mechanical align completed, the

    LED will lid up.

    2) Ensure that distance from laser to stacker is 43mm

    3) Tight up laser module front 2 bolt. ( Not too tight )4) Place Polaroid

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    5) Turn down the target

    6) Slowly Rotate the laser until intensity of the spot is minimum. Then, tight up laserfront 2 screw

    7) Remove the polarizer, main lens and main detector

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    8) Adjust both laser back clamping screw until the beam is center to the hole. Then,

    tight it up.

    9) Take the laser monitor out

    10) Place in the spatial filter without pin hole. Then, place back the laser monitor.After that, position the laser assembly spatial filter until the beam is center at main

    detector. Finally, tighten up the spatial filter.

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    11) Place in the pin hole into spatial filter. Then, align pin hole focusing by using

    business card. ( You may move the laser monitor stack to aside if you feelincomfortable )

    How To Do It

    1) Loosen both A screws and C screw.Ensure Y Spring Screw is just nice

    touching the pin hole surface.

    2) Put in the pin hole and tight it a little bitwill do ( 1 round ). Adjust both B set

    screw until the beam is most bright.

    3) Continue rotate in the pin hole until appfully in to the spatial filter. Then, play

    around with both set screw until the beam i

    clean without flare.

    4) Fully tighten C screw. Then, adjust bothB set screw if beam spot is flare.

    5) Slowly tighten both A screw by little

    little bit each. Important, , dont fully

    tighten the screw in one short. Else, youmay not able to get good beam spot.

    12) Place back the main lens. Adjust the position of main lens by referring to the

    beam toward main detector center. Then, ensure that main lens is parallel to thebase. The reason we need to ensure this is so that the blue light shine in correct

    position/location.

    A

    B

    B

    C

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    13) Misalign the diod carriage by click on MISALIGN command button

    14) Loosing the lens focusing set screw. Then, using focus alignment tool align thelens focusing until the spectra pattern is BOILING.

    Below are the war for adjusting lens focusing

    1) Loosing the lens focusing set screw.2) Unscrew the lens until the lens almost come out.

    3) Continue tighten the focusing lens inward until the spectra getting larger. Carry

    on until the spectra getting smaller. ( Thats the point that almost to optimizing )

    4) Continue align focus until the spectra moving horizontal and vertical parallely.

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    Fine positioning of the beam

    A good alignment will show a display similar to the one below, showing light

    falling onto detectors within detector position 10.

    This means that the laser beam is passing through the hole in the detector ontodetector 0

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    Checking RED Light Alignment

    1) Remove out both cell window from assembly2) Attach the blue light lens as per below:-

    3) On Laser. Make sure that the light as per below.

    4) If its not center, loosen the 2 screws at spatial filter ( Not 2 screw which tighten

    the plate ).Then adjust the spatial filter until the red light is center. Finally, tighten it up once

    you are satisfy.

    a) Leave the other side of cell

    window assembly emptywithout any cell window.

    b) Attach the blue light lensc) Attach the cell window

    holder. Remember to

    remove out the cell window

    lens and O-ring beforeattach.

    Fig: The red dot/circle mustbe in between big square.

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    Blue Light Focussing And Alignment/Position

    1) Off the Laser and ON the Blue Light.2) Perform blue light focusing.

    Loosen A set screw

    Take out the whole blue light.

    Fully loosen C set screw. This C set screw is only act as

    stopper

    Attach back the blue light assembly Move the blue light UP/DOWN as per red arrow until the blue

    light focus is good/sharp

    Lock the A set screw. Then, lock the C set screw which act asstopper.

    3) Adjust the blue light position:-

    Loosen both B screws.

    Then adjust it.

    -

    CB

    B

    A

    Fig: GOOD FOCUSING

    1mm

    Ideally, the blue light intensity suppose to be

    app 50 80%.

    If intensity no good, it could be due to lens

    contaminate. Or as per below:-

    a) Intensity greater than 80%, then LED got

    to in further

    b) Intensity less than 50%, then LED got to

    out further

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    SIDE SCATTER ALIGNMENT

    1) Block the detector with jig to prevent accidentally damage. ON the laser.

    2) Install the side scatter jig as per figure. Then, loosen the 3 screws.

    3) Move the jig forward until touching protection window module.4) Align the front portion and back portion to most higher intensity ( one at a time

    which start from right ). The laser intensity supposedly to be app 41% after

    remove out the detector jig/cover.

    5) Then, you are done. Remove out the side scatter jig after tighten up the 3 screws.

    3 screws

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    Large Angle (LA) Alignment

    1) Launch the diagnostic software

    2) Click on Detector Watch3) A message box will prompt out next. Select NO.

    4) Then, type 43 and click OK

    5) Then, type 44 and click OK

    6) Observe the value for detector 43 and 44 and its very low.

    7) Add in 0.15um latex std into cell. Youll see the detector 43 and 44 value

    significantly increase.

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    8) Loose the 2 screws on the Large Angle board and align/move around until the

    signal is having most higher value. ( Detector 43 ).9) Repeat the same procedure on detector 44 as well.

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    BLUE LIGHT RECEIVER Alignment

    The blue light should be set up in the same way as the large angle detectors.

    Switch off the laser in sys_setup3 and turn on the blue light.

    Click on Detector Watch

    A message box will prompt out next. Select NO.

    Then, type 58

    Add in 0.15um latex std into cell. Youll see the detector 58 value significantly

    increase.

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    Loosen the screws shown and move the blue light detector in a horizontal

    direction to peak the counts.

    When the counts are maximised in this direction peak the counts in a verticaldirection by loosening the screw shown here.

    Loosen up these 2screw

    Loosen thise 2 screw

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    EEPOT CALIBRATION

    Note: this procedure must be done on a Clean, Aligned system with Covers on

    For old MS2000

    1. Select: Tools/Engineering/ Set up EE pots

    2. Select: Tools/Basic/ Open program file/ Sys-setup3.BAS and run the program

    3. Turn the Laser On

    4. Check with Manual align on the sys-setup3 page that the system is aligned

    5. Set all the EEpot values to 32 that is 32 32 32 32

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    6. Select Detector watch in the sys-setup3 window

    7. The program will ask if you want detectors 0 and 53 say NO

    8. Select detectors 60 and 53

    9. Adjust the Gain channel EEpot until channel 60 on the sys-setup page reads

    32768 or as near as possible

    10. Adjust the Laser gain EEpot until channel 53 on the sys-setup3 page reads

    50,000

    11. Adjust the Channel 0 gain EEpot until the laser power displayed on the sys-setup3 page reads 80% or as near as possible

    12. Stop the sys-setup page by clicking on the Red Cross on the top of the page

    13. Turn off the laser

    14. Select: Detector Watch when the prompt asks you if you want detectors 0 and 53say Yes.

    =

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    15. Change the OFFSET EEpot until all the data channels shown on the sys-setup3

    page fall as low as possible but no channel should be lower than 40 units.

    16. Stop the page, turn the laser back on.

    17. The EEpots are now set up. For the LF system the only difference is that the

    gain channel is fixed and cannot be changed.

    The Y value should

    less than 40

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    EEPOT CALIBRATION FOR 2000 LF SYSTEM

    For the LF systems

    1. Make sure that covers are on, the system is aligned and clean water is in the cell

    2. Turn the laser on using the system setup programme

    3. Set the EE pots to 0, 45, 32, 32

    (The gain on the LF systems is fixed and so the value of the Gain EEpot does not

    matter this is why it is set to zero)

    4. Using detector watch select channels 0 and 53

    5. Using the EE pot control dialogue set the laser power (Channel 0 gain) to 80% or

    as near as possible.

    6. Using the EE pot control dialogue set the laser gain (channel 53) to 50,000 or as

    near as possible.

    7. Close the display and then using the Sys_setup page turn off the laser.

    8. Using detector watch select channels 0 and 53

    9. The laser power (channel 0) should now be showing 0% indicating that the laser

    has been blocked.

    10. Using the Offset control in the EEpot control dialogue reduce the background

    level until all detectors are as low as possible but no detector reads below 40 units.

    The EEpots are now set. Close the EEpot dialogue and display. Remember to open

    the laser shutter using Sys_setup.

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    CALCULATE STORING DATA- Basically it divided into 2 parts:-

    o FIELD 3 ( for calibrate linearity. You need latex 155 nano and 1um )

    o FIELD 4 ( for verification. You need latex 9um, 1um, 155 nano meter )

    o In order to save the latex before proceed FIELD 4, you can verify it with

    300nano meter latex )

    Step to Perform FIELD 3

    1) Create virtual drive by:-- Launch MSDOS. Then type:- subst a: c:\caldata

    - Copy all the files inside PSW0047 folder into a:\ (Its recommend to get latest data fr

    extranet before perform this step )

    2) Make sure that the system is align properly with a good background.3) Run the macro which keep inside a:\Field3PM(Latex).bas

    4) Just follow the macro wizard. Itll require you to key in s/n, focal plane s/n, sidescatter board s/n and etc just as for system information only.

    5) In the wizard, latex batch number and lot number need to key in precisely

    according to the certificate given.

    Eg:- Batch = 3150-022 ; Lot # = 35991

    6)