IMMEDIATE ~ i l :~ i~ AVAILABIUTY

Mouse Anti-theta Serum (AKR ASCITES ANTI'C3H)

DESCRIPTION Anti-theta serum (anti-8 serum) is

produced in AKR mice immunized with thymocytes from young C3H mice. Since

these mouse strains are syngeneic at the H-2 locus, this procedure raises

antibodies against the allogeneic C3H theta antigen specifically associated with

thymus derived lymphocytes (T-cells).

Please direct orders or inquiries to:

Mr. Richard D. Eisenberg Marketing Manager

CHARACTERIZATION Each lot of anti-theta serum is precisely characterized the following ways: • Specific cytolytic activity against

C3H Thymocytes C3H Spleenocytes AKR Thymocytes C3H Bone Marrow Cells

• Specific absorption reactions with C3H Brain Cells AKR Brain Cells C3H Kidney Cells

• Effect on mitogenic response to Ph~tohemaglutinin Li popolysaccharide

BION TICS Laboratory Products

Litton 5510 Nicholson Lane, Kensingtonl Maryland 20795

Telephone: (301) 881-5600

M a d e e a s y . . . Until Cordis developed its Complement Com- ponent Test Sets, it just wasn't possible for most laboratories to measure the functional activity of complement components. Technologists had to cope with difficult and complicated on-site preparation of components or use radial im- munodiffusion tests, which measure only the presence of complement components and not the activity.

Now Cordis Complement Component Test Sets make it practical to perform assays for all 9 components in your own laboratory. These sets offer everything you need for accurate, functional analysis by hemolytic assay. All elements are standardized for optimum specificity and sensi- tivity.

Cordis Complement Component Test Sets in- clude component reagents, stable cellular inter- mediates, buffers and complete, easy-to-follow instructions. Cordis also offers all laboratory equipment and supplies required for com- plement assay work. For more information on the Cordis Complement Component Test Sets, fill out and mail the coupon or call our toll-free "800" number.

a n d e v e n e a s i e r You can send specimens to the Cordis Comple- ment Reference Service for analysis by our highly-trained staff. We can assay for whole complement, for any or all complement com- ponents and for 2 complement component inactivators. Assays .are performed either in tubes or microtiter plates as you specify. Results are airmailed to you (telephoned on request) within 3 working days after we receive the specimen. We supply a postage-paid insulated mailing carton, specimen vials, report forms and instructions.

0e dlis. Cordis Laboratories a division of Cord is Corporation P.O. Box 370428, Miami, Florida 33137 Toll-free phone (800) 327-7621

Fill out and mail the c o u p o n - o r call our toll-free "800" number- for complete information on the Cord is Complement Reference Service, Complement Component Test Sets, supplies or equipment.

B B m m ~ m m m ~ m m D m m ~ m , ~ ,

Mai l tO." Cordis Laboratories, P.O. Box 370428, Miami, FL 33137 I-I Please send me complete information on the Cordis Complement Reference Service. [ ] Please send complete information on Cordis Complement Component Test Sets.

NAME

INSTITUTION

TITLE

PHONE

ADDRESS

CITY STATE ZIP

New Phadebas lgE Test makes IgE testing simpler, faster and more

convenient than ever before possible

r "

r

: Radioimmunoassay . ; based on the solid phase Sephadex principle

s uCO1 f cmnPmoer te re teeagneatnt s m, overnight incubation at room temperature

I Wide range measures serum levels 10 to 4000 U/ml

Pharmacia Laboratories Inc.

i

800 Centennial Avenue, Piscataway, New Jersey 08854

Please send me further information on the following Phadebas tests

[ ] B12 [ ] IgE [ ] Insulin [ ] Amylase

I I I I I I L

PHARMACIA LABORATORIES INC. 800 Centennial Avenue, Piscataway, N.J. 08854 Pharmacia (Canada) Ltd. 2044 St. Regis Boulevard Dorval Quebec. Canada (514] 684-8881

Name and Title

Affiliation

Address

City State

m m m m m

zip ~ Pharmacia • . ! m m m m m m m m m m n

THERE ARE TWO WAYS TO LOOK AT R.I.D.

IMMUNO-PLATE°IIITESTS GIVE YOU IN ONE PLATE.

BOTH

End-point or measured-time. Now there's a one-plate radial immunodiffusion system that lets you run both.

Three different reading times, too. 48-hour precision. 16-18 hour overnight. Even 4-hour star.

Two different technics plus three-speed flexibility makes a big difference in testing convenience. Now you can use a single test system that does the job of two. Plus you get 4-hour test capability for stat orders or screening purposes.

And to make it even easier, our new test system lets you forget time-consuming serum dilutions. For major tests within normal adult working ranges, they're simply not required.

The Immuno-Plate flf immunodiffusion system is offered in a broad range of serum protein tests. All with two-technic, three-speed adaptability. All in a convenient six-well plate that reduces waste.

No matter how good you think your present test is, you'll have to agree ours is one better. Maybe two.

Talk to your Hyland representative for all the details. Directions for use are provided in each package. These directions should be read and understood before using. Particular attention should be paid to all warnings and precautions. Should you have any cluestions;call your Hyland representative or call toll-free (800) 854-3235. In Alaska, California, Canada and Hawaii call (714) 540-5000.

~ H Y L P , N D

THE DISCOVERY PEOPLE

Before you renew your contract

for

R E A G E N T S for

A U T O M A T E D P R O C E D U R E S consider Sigma

Sigma offers one of the most complete selections of reagents for AutoAnalyzers e, SMAe and AA II e systems.

Quality is excellent -- Service is unexcelled.

As a basic manufacturer of NAD, Diaphorase, Phosphocreatine, p-Nitrophenyl Phosphate, Malic Dehydrogenase, Lactic Dehydrogenase and many more of the most important enzymes and substrates needed in the formulation of reagents for automated procedures, Sigma is in a unique position to guarantee complete satisfaction.

For complete descriptions, request our special catalog of clinical reagents. If you need any reagent we do not list, please call for current status -- we are continually expanding our offerings.

A L L S I G M A R E A G E N T S A R E U N C O N D I T I O N A L L Y G U A R A N T E E D

Also available:

R E F E R E N C E S E R U M F O R S M A P R O C E D U R E S One set consists of 50 ml. (dried) Reference Serum

and sufficient Diluent for reconstitution. Product No. R 3626

C O N T R O L S E R U M ( U n a s s a y e d ) One set consists of 10 ml (dried) Control Serum

and sufficient Diluent for reconstitution.

Product No. C 0262 Product No. C 0387 (normal to borderline) (borderline to elevated)

Reference and Control Serums contain: Sodium, Potassium, Chloride, CO2, Calcium, Inorganic Phosphate, Glucose, Urea Nitrogen, Uric Acid, Cholesterol, Total Protein, Albumin, Total Bilirubin, Alkaline Phosphatase, LDH, SGOT, and Creatinine. o o o

Other manufacturers o f Automated Reagents as well as laboratories that prepare reagents for their own use might prof i t by calling Lou Berger or Dan Broida at Sigma.

9/74 ORDER DIRECT

TELEPHONE COLLECT

from ANYWHERE in the WORLD Station to Station, 314-771-5750

(Including Saturday ond Sunday until 1 P.M.) - - 0 - -

Night, Person to Person, Dan Beo~da, 3 1 4 - 9 9 3 - 6 4 1 8

- - 0 - - Most orders will be shipped within hours

TWX [Teletypei-Day or N~hl: COLLECT 910-761-0593 TELEGRAM: SIGMACHEM, ~ in t louis, Missouri

~ ~ The Research Laboratories el

C H E M I C A L C O M P A N Y

division of SIGMA ~NTERNATIONAL, LTD

P.O. BOX 14508 • SAINT LOUIS, MISSOURI 63178 U.S.A.

~ A N U F A C T U R E R S O F THE FINEST B L O C H E M I C A L S AVAILABLE

Distributed through: SIG~UI, LONDON Chern Co Ltd • Norblton Stabon Yard Kingston upon Thames, Surrey. KT2 7BH, England

Telephone 01 549 317| (Reverse Charges)

SIGMA Chmie GmbH Mi'INCHEN • DS0]4-Neubiberg. Isarstrasse 14, W Germany Telephone: 089/ 60 70 03 (Reverse Charges)

I !

F

~m t1"

The main point is that our unique design makes the Bio/Physics Cytograf and Cytofluorograf clog-resistant and self- cleaning. Unlike conventional counters, our instruments have no "sticking point" in their cell flow systems. Consequently, there is no longer any need to sacrifice resolution to prevent clogging. Each cell sample enters the flow chamber through the center of a laminar sheath of water. The water surrounds the sample and contains it without mixing. As the stream-within-a-stream passes through the flow chamber, the (outer) water con- stricts the (inner) sample, narrowing it to just 30/~ and accelerating its flow. Thus, the cells pass single-file through the 20/1. thickness of a focused laser beam, at a rate of about 150,000 cells per minute. Whether you're taking a routine cell count or performing an exacting analysis such as DNA per cell distribution, Bio/Physics instruments offer you high resolution, without aggravating interruptions of flow. There are many other advantages we could point out about our counters. But we'd rather let you discover them. By seeing one of our instruments in action, and getting more information. Send today for our helpful guide to counting, sizing, differentiating, characterizing and measuring cell constit- uents. Bio/Physics Systems, Inc., Baldwin Place Road, Mahopac, New York 10541. (914) 628-7451.

000 ~ ' 000 0 ~ ~ ......... j

BiO/PHYSICS SYSTEMS, INC. © 1974---BIO/PHYSICS SYSTEMS, INC.

, ' / / _

IW, I, AI' I|IIG I"ttll Now-Any laboratory can deliver results within hours

In t roduc ing. . . a specific test for human erythropoietin (ESF) which provides de- finitive results 50 TIMES FASTER than bioassay methods. JCL Clinical Research Corporation has developed a fast, simple immunochemical test, utilizing the prin- ciple of hemagglutination-inhibition (HAl), which has made it possible to assay ery- thropoietin levels in almost all clinical laboratories. 1,2 The usefulness of ESF quantitation has previously been limited by the cumbersome, time consuming and expensive bioassay methods available for carrying out such determinations. On the other hand, the new HAl test for human erythropoietin is relatively simple, can be performed in a short period of time, is reproducible, economical and sufficiently sensitive to detect extremely small dif- ferences.

The level of plasma or serum erythro- poietin offers a reliable means of classi- fying diseases of the red blood cell. 3,4

This new JCL Erythropoietin Test Kit is available from Scientif ic Products, Division of American Hospital Supply Corporation.

CODE .~: DESCRIPTION 24 TEST SCREENING KIT: REAGENTS,

"U" PLATES AND INSTRUCTIONS. JCL 1-O01 SUITASLE FOR 8 QUANTITATIVE

TESTS. 48 TEST SCREENING KIT: REAGENTS AND INSTRUCTIONS, SUITABLE FOR

JCL 1-002 16 QUANTITATIVE TESTS, NO "U" PLATES INCLUDED.

96 TEST SCREENING KIT: REAGENTS AND INSTRUCTIONS. SUITABLE FOR

JCL 1-003 32 QUANTITATIVE TESTS. NO "U" PLATES INCLUDED.

1. Lange, R.D., McDonald, T.P., Jordan, T.A., Mitchell, T.J. & Kratch- mar, A.L., "The Hemagglutination-lnhibition Assay for Erythropoietin - - I ts Specificity, Reproducibility and Sensitivity. Israel J. of Med. Sci. 7:861-872, 1971.

2. Lange, R.D., McDonald, T.P., Jordan, T.A., Trobaugh, R.E., Jr. Kretchmar, A.L., and Chernoff, A.I. The Hemagglutination - - Inhibi- tion assay for erythropoietin: A progress report. In HEMATOPOIETIC CELLULAR PROLIFERATION Stohlman, F. Jr., Ed. Grune and Strat- ton, New York, 1970, pp. 122-132.

3. Hillman, R.S. and Finch, C.A., -Erythropoiesis: Normal and Abnormal Seminars in Hematology, 4:327-336. 1967.

4. Levin, W.C. and Alperin, J.B. An Endocrinologic Classification of Polycythemia. Based Upon the Production of Erythropoietin. Am. J. Med. Sci. 256: 131-135, 1968.

For more information,

please call or write:

JCL Clinical Research Corporation Straw Plains Pike Route 6 Knoxville, Tennessee 37914 Telephone: (615) 546-0654

THE J O U R N A L OF I M M U N O L O G Y

RHODAMINE CONJUGATES

IgG FRACTIONS OF STRONG ANTISERUMS ARE' CONJUGATED WITH

TETRAMETHYLRHODAMINE ISOTHIOCYANATE AND CHROMATOGRAPHED

THESE PRODUCTS ARE CONCENTRATES

Rhodamine Conjugated Anti-Human IgA

Rhodamine Conjugated Anti-Human IgG

Rhodamine Conjugated Anti-Human IgM

Rhodamine Conjugated Anti-Human Immunoglobulins (IgA+lgG+lgM)

Rhodamine Conjugated Anti-Human IgE

Rhodamine Conjugated Anti-Human Albumin

Rhodamine Conjugated Anti-Human Complement C3

For versatility use as the first antibody

serum. Then use one of the following

the indirect test:

any specific Cappel anti-

Rhodamine Conjugates for

Rhodamine Conjugated Anti-Goat Gamma Globulin

Rhodamine Conjugated Anti-Rabbit Gamma Globulin

Rhodamine Conjugated Anti-Sheep Gamma Globulin

MANY OTHER RHODAMINE CONJUGATES ARE AVAILABLE

Specialists in Fluorescein, Rhodamine, Ferritin and Peroxidase Conjugations.

c a p p e l l a b o r a t o r i e s , inc. O O W N I N G T O W N , P A . 1 9 3 3 5 , U . S . A .

phone: (215) 269-0275 (215) 269-0500

....... : ~ . , ~ . . . . . . . .

~,VAILABLE ALSO: IMMUNO-DOUBLE DIFFUSION PLATES, NEW SOLID PHASE 2ND ANTIBODY FOR RIA.

O ) ALPHA GAMMA LABS

160 E. Montec i to Ave. • Sierra Madre ° CA 91024 (213) 355-3014

1 5 . S e c o n d =

P r i n t s ............ "l Cordis ~ N Immuno- Diffusion Camera ~ ~ Ideal for photographing in-progress or final results of 1 and 2 dimensional IDF, immuno- and mem- brane disc electrophoresis, hemagglut inat ion, pregnancy tests, blood typing, bacterial sensitivi- ties and counts. Yields clear, sharp Polaroid prints of wet, dry, stained or unstained translucent mate- rials. Faint or closely-spaced precipitin lines are easy to see and interpret because you can select light- or dark-field il lumination at 1.9 or .9 magnifi- cation. Supplied with light diffuser, film, position- ing adapters for slides and plates. Write or call for techn ica l l i terature, demonst ra t ion in your laboratory.

~ ( ~ i ~ Cord is Laborator ies

® Miami, Fla, 33137 Toll-free phone: (800) 327-7621

count on Behring's RID End Point System! With Behring Diagnostics' end point system you may be assured of obtaining the most accurate and reproducible radial immunodif- fusion measurement available. Based on the t e c h n i q u e of M a n c i n i , C a r b o n a r a and Heremans 1, Protein antigens diffuse to end point and no further. Neither do they stop short of accuracy. Furthermore, precipit in rings can be read at your convenience. At 6 hours an early read- out may be obtained for such determinat ions as IgG and IgA. Once end point is reached precipit in rings remain s tab le i nde f i n i t e l y under p rope r s torage. With accuracy and stabil ity such as this, econ- omy and convenience can be yours. As a t ime and money saver, ful l-plate usabil ity does not require that all wells be fi l led at once.

For end point protein quantitation, 30 differ- ent plates are available. This is the widest selection you wil l find and it offers determina- tions for which Behring Diagnostics is the only commercial source. I M a n c i n i , G., C a r b o n a r a , A. O. a n d H e r e m a n s , J. F. I m m u n o - c h e m i s t r y 2 :235 , 1965

Tri-Partigen ~ - IgG, IgA, IgM M-Part igen ~ - Prealbumin, a~ Acid Glycoprotein, Gc Globulin, a2 HS Glycoprotein, Haptoglobin, a 2 Macro- globulin, Hemopexin, C3c (3,A//],C Globulin),/32 Glycoprotein I, a l Antitrypsin, Transferrin, Ant i thrombin III, Ceruloplasmin, Plasminogen, Fibrinogen, Albumin, C4 (3~ E Globulin),/3 Lipoprotein, C3-Activator. S-Part igen ~ - IgA, IgM LC-Partigen t - IgG, IgA, IgM, IgD, CRP and IgE

B D - 3 4 7

] 'he company with a history.., and a future.

~]S / /~ I Behring Diagnostics Oept of Hoechsl Pharmaceulicals. lnc • Route 202-206 North • Somerville N J O8876

GIVE THE WORLD A HELPING HAND. MAIL YOUR (

~RE-NEW YORK. NY tOOl6 ORAl

rI.D PLITERIS'.ED DIrI.TID FIfiY I t ' s t h e b e s t b e c a u s e i t b e s t d e f i n e s t h e m e d i c i n e t h a t y o u p r a c t i c e

The new edit ion contains:

• 7 ,199 signif icant new vocabulary entries

• 24, 877 important editorial revisions

• excellent color plates and i l lustrations

• an alphabetical index to subentries

• appendices, tables, eponyms, and synonyms

• a special section to help you get the most f rom

your dict ionary

Beautiful ly bound and thumb- indexed for easy use,

the new Stedman's wil l be the most valuable addi-

tion to your library in years

1 9 7 2 / 1 , 6 2 0 p a g e s / S 1 8 . 5 0

THE W I L L I A M S AND WILKINS C O M P A N Y 428 E. Preston Street Balt imore, Maryland 21202