MORPHOLOGY OF HETERODERA FILIPJEVI FROM · PDF fileINTRODUCTION Cereals were grown in Serbia...
Transcript of MORPHOLOGY OF HETERODERA FILIPJEVI FROM · PDF fileINTRODUCTION Cereals were grown in Serbia...
Violeta Oro, Svetlana Živković, Tatjana Popović,
Nenad Trkulja, Žarko Ivanović
MORPHOLOGY OF HETERODERA FILIPJEVI
FROM SERBIA
Belgrade, 2012.
Nematology Laboratory, Institute for Plant Protection and Environment, Belgrade, Serbia,
INTRODUCTION
Cereals were grown in Serbia since the first
humans came, data from the 14th century and
Marko Kraljević mentioned wheat growing
Nowadays, wheat, barley, triticale & oats are
grown on ca: 600 000 ha and maize on 1,3M ha
The most globaly recognized and economically
important nematode on wheat is Cereal Cyst
Nematode (CCN) complex. Three main species
Heterodera avenae, H. filipjevi & H. latipons
are the most economically important
INTRODUCTION
In our country only H. avenae was recorded
Heterodera filipjevi is a new economically
important parasite on wheat for Serbia
The morphology of cysts and second stage
juveniles (J2) of H. filipjevi are described
MATERIAL & METHODS
Heterodera filipjevi cysts were found in soil
samples from Gunaroš (Vojvodina province)
Cysts were extracted with Spears apparatus &
collected on 150 mm sieve, J2 by dissection
Identification by morphology: cyst body length
L, width W, L/W ratio, fenestral length l,
width w, l/w ratio, vulval bridge length &
width, underbridge length, presence of bullae,
J2 length & width, stylet length & shape, tail
length & width, hyaline tail length
RESULTS & DISCUSSION
Cysts are lemon shaped with posterior
protuberance, golden brown
Vulval cone is bifenestrate with horseshoe-
shaped semifenestra, bullae & underbridge
J2 has an offset head, stylet with anchor-
shaped basal knobs
Posterior region of J2 has tapering tail with a
rounded tip
RESULTS & DISCUSSION
The shortest juveniles from Norway, the
longest from Oregon (USA)
The longest stylet from Turkey, the shortest
from Serbia
The longest tail from Serbia, the shortest tail
has type population
The longest hyaline portion of the tail has
Gunaroš population, the shortest hyaline has
population from Iran
RESULTS & DISCUSSION
All populations except from UK have L/W
ratio higher than specimens from Serbia
The highest fenestral length has Turkey, the
lowest from Norway; the highest fenestral
width from Serbia, the lowest from Norway
The longest vulval slit from Serbia, the
shortest has type population
The longest underbridge Serbia, the shortest
Oregon
CONCLUSIONS
In relation to type population, J2 from Serbia
are longer, have the longer tail and hyaline
portion of tail and a shorter stylet.
Cyst morphometrics regarding fenestral
length & width, vulval slit and underbridge
comparing to type population are greater.
Only cyst length/width ratio is smaller.
H. filipjevi is designated the most economically
important species of CCN complex, important
for Serbian phytosanitary regulation.