More images at http://astro.temple.edu/~sodicm/labs/BloodWeb/sld001.htm

What cell line? “fluffy,” “brushed”= erythropoietic cell line “dots”, “ink blobs”= granulocitic cell line

A blast is a blast, no matter what cell line Reticulocyte versus Erythrocyte

PRONORMOBLAST FEATURES

A blast is a blast, don’t worry about the type

Mitotic

BASOPHILIC NORMOBLAST FEATURES

Coarser nucleus Mitotic Blue cytoplasm, from

what? No Granules! 2-3X larger than

surrounding RBCs

POLYCHROMATIC NORMOBLAST FEATURES

Slightly larger than RBCs

Mitotic “checker board” No Granules!

ORTHOCHROMATIC NORMOBLAST FEATURES

Same size as surrounging RBCs

NOT Mitotic “pyknotic nucleus” “eccentrically placed”

just a buzz word Reddish purple

cytoplasm. Why? No Granules

RETICULOCYTE FEATURES

No nucleus NOT Mitotic Contains

“granulofilamentous material” what kind?

Residual RNA and mitochondria in cytoplasm

Where does development continue?

MYELOBLAST FEATURES

Non-granular cytoplasm

Mitotic Unipotent 2-3X larger than

surrounding RBCs A blast is a blast

PROMYELOCYTE FEATURES

Primary Granules, “azurphilic”

Mitotic Granules, but no

dawn of neutrophilia

NEUTROPHILIC MYELOCYTE FEATURES

Nucleus is round, slightly indented

“Dawn of Neutrophilia”

Secondary granules (primary granules still there) “specific granules”

mitotic

NEUTROPHILIC METAMYELOCYTE FEATURES

Indented nucleus (“kidney bean shaped”)

Neutrophilic (specific, secondary)> azurphilic (non-specific, primary)

NEUTROPHILIC BAND FEATURES

Indented nucleus “horseshoe shaped” Neutrophilic

(specific) granules Can be found in

circulation

NEUTROPHIL (SEGMENTED, PMNL) FEATURES

2-4 lobed, thin link of what?

Neutrophilic granules>>azurphilic granules

PRONORMOBLAST MYELOBLAST

BASOPHILIC NORMOBLAST PROMYELOCYTE

MYELOBLASTNEUTROPHILIC MYELOCYTE

…it is tough to miss What cell did this

come from, what is it and what comes from this cell?

Location?

Define Hct. Nl. values? Romanovsky stain- other name, use A cell is stained and said to be almost

totally basophilic with a little acidophilia in the nucleus. What is the charge on the dye staining the

majority of the cell? What about the dye staining the acidophilic

parts of the nucleus?

T-cell versus B-cell, which is more prevalent in peripherial circulation?

How would you tell? What is a “left shift?”