Molecular microbiology real time pcr

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Design, development and validation of a Real Time PCR for thediagnosis of Cryptosporidium parvum.Design, development and validation of a Real Time PCR for thediagnosis of Chlamydiaceae.Design, development and validation of a Real Time PCR for thediagnosis of Para-Chlamydiaceae.Design, development and validation of a Real Time PCR for thediagnosis of Rotavirus type A.Design, development and validation of a Real Time PCR for thediagnosis of Pan-Pestivirus. Design, development and validation of a Real Time PCR for thediagnosis of Border Diseases virus (BDV).Design, development and validation of a Real Time PCR for thediagnosis of Brachyspira hyodysenteriae.Design, development and validation of a Real Time PCR for thediagnosis of Brachyspira pilosicoli.Design, development and validation of a Real Time PCR for thediagnosis of Brachyspira intermedia.Design, development and validation of a Real Time PCR for thediagnosis of Myxomatosis virus.Design, development and validation of a Real Time PCR for thediagnosis of Streptococcus suis ser 9.Design, development and validation of a Real Time PCR for thediagnosis of Streptococcus suis ser 2.


Design, development and validation of a Real Time PCR for diagnosis of Porcine Circovirus type 2 (PCV-2).Design, development and validation of a Real Time PCR for thediagnosis of PRRSV EU strain.

Design, development and validation of a Real Time PCR for the diagnosis of Bovine Herpesvirus type-1 (BHV-1).Design, development and validation of a Real Time PCR for thediagnosis of Chlamydia suis. Design, development and validation of a Real Time PCR for thediagnosis of Chlamydophila pecorum. Design, development and validation of a Real Time PCR for thediagnosis of Chlamydophila pneumoniae. Design, development and validation of a Real Time PCR for thediagnosis of Salmonella enterica.Design, development and validation of a Real Time PCR for thediagnosis of Theileria sp.Design, development and validation of a Real Time PCR for thediagnosis of Babesia sp.Design, development and validation of a Real Time PCR for thediagnosis of Mycobacterium avium subsp. paratuberculosis. Design, development and validation of a Real Time PCR for thediagnosis of Neospora caninum.Design, development and validation of a Real Time PCR for thediagnosis of Encephalitozoon cuniculi.


Design, development and validation of a Real Time PCR for the diagnosis of Listeria monocitogenes.Design, development and validation of a Real Time PCR for thediagnosis of Tritrichomona foetus. Design, development and validation of a Real Time PCR for thediagnosis of Escherichia coli eae gene.

Design, development and validation of a Real Time PCR for thediagnosis of Toxoplasma gondii. Design, development and validation of a Real Time PCR for thediagnosis of Chlamydophila abortus. Design, development and validation of a Real Time PCR for thediagnosis of Coxiella burnetii.Design, development and validation of a Real Time PCR for thediagnosis of Besnoitia besnoiti Design, development and validation of a Real Time PCR for thediagnosis of Anaplasma marginale msp 5 geneDesign, development and validation of a Real Time PCR for thediagnosis of Mycoplasma agalactiae.Design, development and validation of a Real Time PCR for thediagnosis of pathogenic Leptospira. Design, development and validation of a Real Time PCR for thediagnosis of Mycoplasma bovis.


Design, development and validation of a Real Time PCR for thediagnosis of Mycoplasma pneumoniae.

Design, development and validation of a Real Time PCR for thediagnosis of Mycoplasma hyopneumoniae.

Design, development and validation of a Real Time PCR for thediagnosis of Porcine Parvovirus (PPV).Design, development and validation of a Real Time PCR for the diagnosis of Chlamydophila psittaciDesign, development and validation of GBlock synthetic gene fragments for use as positive and quantification control in infectious microorganisms diagnosis.Design, development and validation of ULTRAMER synthetic gene fragments for use as positive and quantification control in diagnosis infectious microorganisms.

Design, development and validation of Plasmids with fragments of genes cloned for use as a positive and quantification control in Real Time PCR kits.

Design, development and validation of a Real Time PCR assay for the recognition of the plasmid used as the extraction exogenous control.


Design, development and validation of GBlock synthetic gene fragments for use as positive and quantification control in infectious microorganisms diagnosis

Design, development and validation of ULTRAMER synthetic gene fragments for use as positive and quantification control in diagnosis infectious microorganisms.

Diseases of the community. Real Time PCR for Human respiratory entitiesInfrastructure and equipping of laboratories rooms for productions of Real Time PCR kits.

Experience in Training of technical staff to: run the diagnosis of infectious diseases by Real Time PCR; Validation of trials and quantification controls, well as extraction of nucleic acids.Validation of several master mix (Mix probe) and one-step Reverse Transcription kit for to development PCR Real Time assaysDesign, development and validation of Plasmids with fragments of genes cloned for use as a positive and quantification control in Real Time PCR kits

I have experience with a variety of commercial kits for Real Time PCRfrom different biotechnological companies. (Applied Biosystems/LSI; Primer design; Vacunek; Adiagene; Genekan; Ingenetix; Ingenasa etc).


Design, development and validation of Housekeeping, endogenous control panels for Real Time PCR in ruminants.

Design, development and validation of Housekeeping, endogenous control panels for Real Time PCR in Rabbits.

Design, development and validation of Housekeeping, endogenous control panels for Real Time PCR in porcine.

Lyophilising positive controls, longevity studies at 4° C. Evaluation and quantification of the lyophilic by Real Time PCR.

Validation of several master mix (Mix probe) and one-step Reverse Transcription kit for to development PCR Real Time assays.

Commissioning and evaluation of nucleic acid extraction LABTURBO automatic equipment. Comparison with some manual extraction kitsReal time PCR (qPCR) for the detection and quantification of microorganisms in the water transmitted.Infrastructure design and equipping of laboratories and boxes forperform infectious disease diagnostic by Real Time PCR.


Molecular microbiology real time pcr

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