Molecular Life Science Review June 15, 2003 Learning objectives- What is sickle cell anemia?...
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Molecular Life Science Review
June 15, 2003 Learning objectives-
What is sickle cell anemia? Increased knowledge of the structure of atoms, molecules, and biomolecules Central Dogma How DNA mutations result in abnormal proteins Protein structure classifications How to perform comparisons of protein sequences
Workshops- Import cytochrome C sequences of different species. Perform Clustal W comparison Find out the molecular basis of sickle cell anemia
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Symptoms of Sickle Cell Anemia
pain episodes strokes increased
infections leg ulcers bone damage yellow eyes or
jaundice early gallstones lung blockage
kidney damage and loss of body water in urine
painful erections in men (priapism)
blood blockage in the spleen or liver (sequestration)
eye damage low red blood cell
counts (anemia) delayed growth
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Hierarchy of particles
Atoms (Eg. C, H, O, N)
Molecules (Eg. H2O, CH4)
small Biomolecules (Eg. Sugars, amino acids)
large Biomolecules (Eg. polysacharrides, proteins)
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Example of ionic bond
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H + H H2
O + O O2
2H2O 2H2 + O2
Chemical Reactions
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Covalent Bonds(electrons shared)
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pH scale. A convenient methodto measure the concentrationof H+
pH = -log[H+]
Remember pH + pOH = 14M = 1 mole/liter
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Amino acid characteristics
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Website for Amino acid interactive Workshop Amino acids
1 2
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DNA sequenceWrite the primary sequence of the DNA displayed in 3Bhttp://www.blc.arizona.edu/Molecular_Graphics/DNA_Structure/DNA_Tutorial.HTML
http://www.rothamsted.bbsrc.ac.uk/notebook/courses/guide/dnast.htm
Website for interactive workshop for DNA analysis
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Interactive display of amino acid and codons
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Translation exercise
Translate the following sequence using the codontable:
ATGGUGCACCUGACUCCUGAGGAGAAG
Perform same procedure using a software program:
http://us.expasy.org/tools/dna.html
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Central Dogma
DNA
RNA
Protein
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DNA
RNA (with ribosomes)
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*
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>gi|1244762|gb|AAA98563.1| p53 tumor suppressor homolog
MSQGTSPNSQETFNLLWDSLEQVTANEYTQIHERGVGYEYHEAEPDQTSLEISAYRIAQPDPYGRSESYD
LLNPIINQIPAPMPIADTQNNPLVNHCPYEDMPVSSTPYSPHDHVQSPQPSVPSNIKYPGEYVFEMSFAQ
PSKETKSTTWTYSEKLDKLYVRMATTCPVRFKTARPPPSGCQIRAMPIYMKPEHVQEVVKRCPNHATAKE
HNEKHPAPLHIVRCEHKLAKYHEDKYSGRQSVLIPHEMPQAGSEWVVNLYQFMCLGSCVGGPNRRPIQLV
FTLEKDNQVLGRRAVEVRICACPGRDRKADEKASLVSKPPSPKKNGFPQRSLVLTNDITKITPKKRKIDD
ECFTLKVRGRENYEILCKLRDIMELAARIPEAERLLYKQERQAPIGRLTSLPSSSSNGSQDGSRSSTAFS
TSDSSQVNSSQNNTQMVNGQVPHEEETPVTKCEPTENTIAQWLTKLGLQAYIDNFQQKGLHNMFQLDEFT
LEDLQSMRIGTGHRNKIWKSLLDYRRLLSSGTESQALQHAASNASTLSVGSQNSYCPGFYEVTRYTYKHT
ISYL
FASTA format
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Multiple sequence alignment Human-locus number AAA35732 Dog-locus number CCDG Yeast-locus number from structure
or protein sequence database 1YCC
CLUSTAL PROGRAM
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Workshop Find out the chromosomal location of the gene
that causes sickle cell anemia. Give the name of the gene. Find out the nucleotide change and amino acid
change that leads to sickle cell anemia (there may be more than one change that gives rise to the disease)
If sickle cell anemia is so devastating, why has it lasted in the population for such a long time? Give a molecular explanation (you may have to do a little research to get this)
Print out answers and show instructor.