GHUCCTS: Translational Technology Resources (TTR)

Molecular Imaging Laboratory (MIL, HU)

Paul Wang, PhD. Director

http://www.howard.edu/medicine/radiology/mil/

Molecular Imaging Laboratory (HU) Provides:

High resolution, high sensitivity MRI – Small animal Bruker 400 MHz (9.4T) 89mm and Varian 200 MHz

(4.7T) MRI/MRS systems – Clinical 3T GE MRI/MRS system – In vitro and/or In vivo studies

Experimental design– Nanoparticle with ligands– Protein or biochemical pathways

Multi-photon confocal microcopy

Molecular Imaging Laboratory (HU) Mission Statement

The mission of the Howard University Biomedical Imaging Core Facility is to train the new generation young scientists, and foster multidisciplinary research using modern imaging technologies, to study the underline disease processes, and to investigate means to alleviate grave suffering.

Molecular Imaging Laboratory (HU) MRI/MRS Systems

Varian 4.7T Horizontal bore

Bruker 9.4T 89 mm bore

MR angiography of mouse brain

MR imaging of mouse heart

In vivo high resolution mouse brain imaging

In vivo mouse white/grey matter of spinal cord

Molecular Imaging Laboratory (HU) Optical Imaging System

(a) (b)Bioluminescence Imaging of Secondary Tumors in Lung, MDA-MB-231-luc cells (BLI able to detect ~ 50 cells in vivo)

Emissionfilter wheel

Excitationfilter wheel

Lensassembly

Opticalswitch

TransilluminationFiber bundle

Scanninglaser

Heated samplestage

CCD, TE-cooled to -90C

10 excitation and18 emission filters

TfNIR-LipNBD-CA Probe for Fluorescense NIR Optical Imaging

Objectives:

To provide state-of-the-art instrumentation, technical expertise and essential services for molecular, cellular, and in vivo imaging

To provide a broader training experience for students and faculty in biomedical imaging through methodology-centered seminars, workshops, mini-courses, and internships

To foster new multidisciplinary research collaborations using modern imaging technologies.

Areas of Investigations:

In vivo MRI and optical imaging of small animals with disease models

Molecular imaging of disease processes at molecular, subcellular, cellular, and live animal levels

Development of nanoparticles for diagnostic and therapeutic use

Novel probe design for targeted drug delivery Ex vivo cell imaging by electron microscopy

Molecular Imaging Laboratory (MIL, HU) Contacts:

Alexandru Korotcov, PhDManager

– Phone: 202-865-3742– Email: akorotcov@yahoo.com

http://www.howard.edu/medicine/radiology/mil/

Support Provided by:

Shared Resources are partially supported by the Georgetown Howard University Center

for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01

In your publications, remember to mention support provided from the

GHUCCTS.