Molecular forensics 2
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Transcript of Molecular forensics 2
![Page 1: Molecular forensics 2](https://reader036.fdocuments.in/reader036/viewer/2022070315/5550330eb4c9059f318b4f7a/html5/thumbnails/1.jpg)
Molecular Forensics
Adapting a Case for Two
Different Course Levels
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Molecular Forensics
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Case Summary
This case is based on a true story
HIV+ dentist in Florida was accused of
infecting several patients
HIV sequences were obtained for the dentist
and the patients
The dentist was sued based on the sequence
evidence
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Goals
Introduce Bioinformatics to students using case-based learning
Suggest methods for upper level undergraduate course, e.g. molecular genetics (300), and lower level course such as microbiology (200)
Demonstrate to students that science is evolving and tools are evolving…science is not static
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Steps for learning
Introduce the case: Molecular Forensics
Look at the “printed” data: HIV sequence data Explore the difficulties of manually comparing sequences
Explore options
Introduce Workbench Align sequences
Pairwise sequence similarities
Rooted and unrooted trees
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Steps for learning – cont.
(In preparation: Module to show students how to
interpret trees)
Use module with students to explore how to
interpret trees
Analyze the case using new skills and come to a
conclusion
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Tree Module - Overview
Explanation of phylogenetic trees What do they show? What don’t they tell you? Are there different types of trees? Why?
Sample trees Correlating taxonomy with trees Practice in interpreting
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Tree ModuleWritten Introduction to Trees
Possible ResourcesPhylogenetic TreesSusan CatesThis work is produced by The Connexions Project and licensed under the Creative Commons Attribution Licensehttp://cnx.org/content/m11052/2.8/
Comparing Phylogenetic TreesModule by: sam donovan This work is produced by The Connexions Project and licensed under the Creative Commons Attribution Licensehttp://cnx.org/content/m15807/latest/
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Sample Trees
Provide students with rRNA sequence data from a variety of organisms, e.g. – Rabbit, frog, fungus and bacteria
Provide rooted and unrooted trees based on this data
Show students corresponding alignment
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Sample Trees
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Clustal WUnrooted Tree
http://Workbench.sdsc.edu
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Clustal WRooted Tree
http://Workbench.sdsc.edu
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Clustal WSequence Alignment
http://Workbench.sdsc.edu
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Taking the Case Farther
Higher level courses..300 level and higher
Compare the HIV from the “dentist forensics” case with HIV sequences from other studies
Look at trees and interpret
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Taking the Case Further
Lower level courses: 200 level and below
Take the rooted and/or unrooted trees produced from the case data and create a mobile which shows the relationships between samples