Molecular epidemiology of lyssaviruses in Eurasia
description
Transcript of Molecular epidemiology of lyssaviruses in Eurasia
1
Molecular epidemiology of lyssaviruses in Eurasia
Dr Lorraine McElhinneyVeterinary Laboratories Agency (Weybridge), UK
2
Previous Published Studies
Russian RABV phylogeny(Kuzmin et al 2004)
NORWAY
SWEDEN
FINLAND
DENMARK
ESTONIA
LATVIA
LITHUANIA
BELARUS
UNITEDKINGDOM
IRELAND
GERMANYPOLAND
UKRAINE
MOLDOVA
CZECHREPUBLIC
AUSTRIA
SLOVAKIA
HUNGARYROMANIA
NETHERLANDS
FRANCE
LUXEMBOURG
SWITZERLAND
SPAIN
PORTUGAL ITALY
SLOVENIA CROATIA
BOSNIA &HERZEGOVINA
SERBIA
BULGARIA
MACEDONIA
ALBANIA
GREECE
ANDORRA
MALTA
MONACO
VATICANCITY
SANMARINO
LIECHTENSTEIN
TURKEY
CYPRUS
GEORGIA
ARMENIA
BELGIUM
MONTENEGRO
West Russia
Russia Steppes & Kazakhstan
WE
EE
NEE
WE
EE
NEE
WE
EE
NEE
WEEE
NEE
CE
WEEE
NEE
WE
EE
NEE
CEWE
EE
NEE
WE
CE
WE
WESF
C
D
C
D
G
C
DG
WE
EE
NEE
CE
C
D
G
SF
TURKEY 1
CE GROUP
NEE GROUP
WE GROUP
EE GROUP
TURKEY 3
TURKEY 2
SERBIA FOX
D
D
C
C
C
C
CD
East Europe
West Europe
Central Europe
Group C
Group D
Georgian
Serbia fox
North East Europe
European RABV phylogeny(McElhinney et al 2006)
Iranian RABV phylogeny(Nadin-Davis et al 2003)
European RABV phylogeny(Bourhy et al 1999)
Lyssavirus phylogeny(Badrane et al 2001)
Middle East RABV phylogeny(David et al 2007)
3
Reported Rabies Cases Serbia & Montenegro 1993-2005
Year
No.
1993
1994
1995
1996
1997 1998
1999
2000
2001
2002
2003
2004
2005
Total 82 46 118 110 98 103 113 152 245 218 232 202 371
Wildlife 53 25 100 81 67 72 84 112 185 182 184 163 264
Domestic 29 21 18 29 31 31 29 40 60 36 48 39 107
Rabies present only in the Northern Regions of Serbia prior to 1990’s (Sava, Danube)Rapid spread of rabies southwards at a rate of 20-50km/year since 1991
4
Places of origin of the isolates collected between 1971-1974 (n=8)
Foxes (n=7), Dog (n=1)
12
3
467
8
913
1415
1720
2122 24
2930
SCSC = Sombor cluster
(5,10,11,12,16,18,19,23,25,26,27,2831)
32
8
1
2
3
4
7 5,6=
5
Places of origin of the isolates collected between 1976-1979 (n=33)
Foxes (n=25), Cats (n=4), Dog, Deer, Cow, Badger (n=1)
12
3
467
8
913
1415
1720
2122 24
2930
SCSC = Sombor cluster
(5,10,11,12,16,18,19,23,25,26,27,2831)
32
12
3
467
8
913
1415
1720
2122 24
2930
12
3
467
8
913
1415
1720
2122 24
2930
SCSC = Sombor cluster
(5,10,11,12,16,18,19,23,25,26,27,2831)
32
SCSC = Sombor cluster
(5,10,11,12,16,18,19,23,25,26,27,2831)
32
6
Places of origin of the isolates collected between 1985-1986 (n=9)
Foxes (n=3), Dog (n=3), Cat, Horse and Cow (n=1)
2, 5
3, 4
1
68
79
7
Places of origin of the isolates collected between 1996-1998 (n=31)
Foxes (n=15), Cats (n=13), Dog (n=3)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
1718
1920
21
2223
24
25
26
27
2829
3031
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
1718
1920
21
2223
24
25
26
27
2829
3031
8
Places of origin of the isolates collected between 1999-2000 (n=66)
Foxes (n=28), Cats (n=20), Dogs (n=10), Cows (n=4), Horses (n=2), Deer, Goat (n=1)
1,8,18,46
2
3,38
4,11,33,40,
505,22
6,49
7,16,36,41,59
9,26,57
10
12,13,14
15
17
19,23,24,51
20
21,44
25,31
27,2928
30
32
34,35,56
37,54
39,42
43,4845
47
52,53,55,58
60
61
6263
64
65
66
1,8,18,46
2
3,38
4,11,33,40,
505,22
6,49
7,16,36,41,59
9,26,57
10
12,13,14
15
17
19,23,24,51
20
21,44
25,31
27,2928
30
32
34,35,56
37,54
39,42
43,4845
47
52,53,55,58
60
61
6263
64
65
66
1,8,18,46
2
3,38
4,11,33,40,
505,22
6,49
7,16,36,41,59
9,26,57
10
12,13,14
15
17
19,23,24,51
20
21,44
25,31
27,2928
30
32
34,35,56
37,54
39,42
43,4845
47
52,53,55,58
60
61
6263
64
65
66
2
3,38
4,11,33,40,
505,22
6,49
7,16,36,41,59
9,26,57
10
12,13,14
15
17
19,23,24,51
20
21,44
25,31
27,2928
30
32
34,35,56
37,54
39,42
43,4845
47
52,53,55,58
60
61
6263
64
65
66
9
Isolate DistributionCountry No of isolates 1970's 1980's 1990's 2000+Bosnia & Herzegovina 7 0 4 3 0Montenegro 11 1 0 1 9Serbia 129 40 5 44 40Total 147 41 9 48 49
147 virus isolates available for studyDates range between 1972-2001 Varied Host species:78 Foxes, 38 cats, 18 dogs, 13 othersAdditional published sequences obtained from Genbank Data Sources e.g.
Kissi et al (1992), Bourhy et al (1999)Vanaga et al (2003), Nadin-Davis et al (2007)
David et al (2007)
10
Rabies Virus Genome
400bp
110.01
RV51 USA fox U22634U22487 U22640RV53 RAVN2756
RV1336RV14204055DGRV303RV277RV61
Africa3U22627DQ837462U22484CVS TakamenU22637
9126MEXU22651U22629DQ837474U42705RV1185
Bulgfox02 RV1194RV1219RV1211RV1153RV1147RV1162RV1151NU42703RV1154
DQ837475RV202DQ837408DQ837385DQ837448DQ837383
DQ837411DQ837412U22480U22482DQ837443U43007
AY277576AF033879RV1334RV259U42605U42701AF033888RV860
U42708U43008RV1189U42706U22839U42994
U42713U42998U43001RV1197RV1198
RV1223RV2028AF033881RV1267RV1308RV1538RV1222RV1268RV1240RV1257RV1251RV1239RV1247
RV1243RV1188RV905RV1168RV1176RV1157RV1161RV1202
RV1207RV1208RV1209RV1255RV1171
RV1192RV1266RV1184RV1289RV1287RV1196RV1292RV1339RV1269RV1234
RV1272RV1274RV1244RV1230RV1237RV1214RV1227 RV341RV338RVU22653
AB178892Thaidog63AB041968AF374721RV108 RV167
RV155
Africa 2ArcticArctic-likeAfrica 3Africa 4Africa 1Japan
Turkey 1FRYBulgaria foxSerbia fox
Turkey 2
Israel
Asia
American bat
NEE (Europe)SteppesCE (Europe)WE (Europe)Bosnia/Montenegro
EE (Europe)
0.01
RV51 USA fox U22634U22487 U22640RV53 RAVN2756
RV1336RV14204055DGRV303RV277RV61
Africa3U22627DQ837462U22484CVS TakamenU22637
9126MEXU22651U22629DQ837474U42705RV1185
Bulgfox02 RV1194RV1219RV1211RV1153RV1147RV1162RV1151NU42703RV1154
DQ837475RV202DQ837408DQ837385DQ837448DQ837383
DQ837411DQ837412U22480U22482DQ837443U43007
AY277576AF033879RV1334RV259U42605U42701AF033888RV860
U42708U43008RV1189U42706U22839U42994
U42713U42998U43001RV1197RV1198
RV1223RV2028AF033881RV1267RV1308RV1538RV1222RV1268RV1240RV1257RV1251RV1239RV1247
RV1243RV1188RV905RV1168RV1176RV1157RV1161RV1202
RV1207RV1208RV1209RV1255RV1171
RV1192RV1266RV1184RV1289RV1287RV1196RV1292RV1339RV1269RV1234
RV1272RV1274RV1244RV1230RV1237RV1214RV1227 RV341RV338RVU22653
AB178892Thaidog63AB041968AF374721RV108 RV167
RV155
Africa 2ArcticArctic-likeAfrica 3Africa 4Africa 1Japan
Turkey 1FRYBulgaria foxSerbia fox
Turkey 2
Israel
Asia
American bat
NEE (Europe)SteppesCE (Europe)WE (Europe)Bosnia/Montenegro
EE (Europe)
0.010.01
RV51 USA fox U22634U22487 U22640RV53 RAVN2756
RV1336RV14204055DGRV303RV277RV61
Africa3U22627DQ837462U22484CVS TakamenU22637
9126MEXU22651U22629DQ837474U42705RV1185
Bulgfox02 RV1194RV1219RV1211RV1153RV1147RV1162RV1151NU42703RV1154
DQ837475RV202DQ837408DQ837385DQ837448DQ837383
DQ837411DQ837412U22480U22482DQ837443U43007
AY277576AF033879RV1334RV259U42605U42701AF033888RV860
U42708U43008RV1189U42706U22839U42994
U42713U42998U43001RV1197RV1198
RV1223RV2028AF033881RV1267RV1308RV1538RV1222RV1268RV1240RV1257RV1251RV1239RV1247
RV1243RV1188RV905RV1168RV1176RV1157RV1161RV1202
RV1207RV1208RV1209RV1255RV1171
RV1192RV1266RV1184RV1289RV1287RV1196RV1292RV1339RV1269RV1234
RV1272RV1274RV1244RV1230RV1237RV1214RV1227 RV341RV338RVU22653
AB178892Thaidog63AB041968AF374721RV108 RV167
RV155
RV1289RV1287RV1196RV1292RV1339RV1269RV1234
RV1272RV1274RV1244RV1230RV1237RV1214RV1227 RV341RV338RVU22653
AB178892Thaidog63AB041968AF374721RV108 RV167
RV155
Africa 2ArcticArctic-likeAfrica 3Africa 4Africa 1Japan
Turkey 1FRYBulgaria foxSerbia fox
Turkey 2
Israel
Asia
American bat
NEE (Europe)SteppesCE (Europe)WE (Europe)Bosnia/Montenegro
EE (Europe)
Phylogenetic Tree (N400bp)
12
Isolates belonging to WE Group
0.01
RV51 USA fox U22634
U22487 U22640 RV53 RAVN2756 RV1336 RV1420
4055DG RV303
RV277 RV61 Africa3 U22627
DQ837462 U22484
CVS Takamen
U22637 9126MEX U22651
U22629 DQ 837474
U42705 RV1185 Bulgfox02
RV1194 RV1219
RV1211 RV1153
RV1147 RV1162 RV1151N
U42703 RV1154
DQ837475 RV202
DQ837408 DQ837385 DQ837448 DQ837383 DQ837411
DQ837412 U22480
U22482 DQ837443 U43007
AY277576 AF033879 RV1334
RV259 U42 605
U42701 AF033888
RV860 U42708
U43008 RV1189
U42706 U22839 U42994
U42713 U42998
U43001 RV1197
RV1198 RV1223 RV2028 AF033881
RV1267 RV1308
RV1538 RV1222
RV1268 RV1240 RV1257
RV1251 RV1239 RV1247
RV1243 RV1188
RV905 RV1168
R V1176 RV1157
RV1161 RV1202
RV1207 RV1208 RV1209 RV1255
RV1171 RV1192
RV1266 RV1184
RV1289 RV1287
RV1196 RV1292 RV1339 RV1269
RV1234 RV1272 RV1274 RV1244 RV1230 RV1237 RV1214 RV1227
RV341 RV338
RVU22653 AB178892 Thaidog63
AB0 41968 AF374721 RV108
RV167 RV155
RV860 (CZECH)AF033888 (POL)
U42994 (GER)
U42706 (BOSNIA 1986)RV1189 (N=6, BOSNIA 1986, MONT 1999-2000)
U43008 (SLOV)U42708 (AUS)
U42605 (FRA)U42701 (GER)
U22839 (SCG)
U42713 (BEL)
7270
97
74
0.01
0.01
RV51 USA fox U22634
U22487 U22640 RV53 RAVN2756 RV1336 RV1420
4055DG RV303
RV277 RV61 Africa3 U22627
DQ837462 U22484
CVS Takamen
U22637 9126MEX U22651
U22629 DQ 837474
U42705 RV1185 Bulgfox02
RV1194 RV1219
RV1211 RV1153
RV1147 RV1162 RV1151N
U42703 RV1154
DQ837475 RV202
DQ837408 DQ837385 DQ837448 DQ837383 DQ837411
DQ837412 U22480
U22482 DQ837443 U43007
AY277576 AF033879 RV1334
RV259 U42 605
U42701 AF033888
RV860 U42708
U43008 RV1189
U42706 U22839 U42994
U42713 U42998
U43001 RV1197
RV1198 RV1223 RV2028 AF033881
RV1267 RV1308
RV1538 RV1222
RV1268 RV1240 RV1257
RV1251 RV1239 RV1247
RV1243 RV1188
RV905 RV1168
R V1176 RV1157
RV1161 RV1202
RV1207 RV1208 RV1209 RV1255
RV1171 RV1192
RV1266 RV1184
RV1289 RV1287
RV1196 RV1292 RV1339 RV1269
RV1234 RV1272 RV1274 RV1244 RV1230 RV1237 RV1214 RV1227
RV341 RV338
RVU22653 AB178892 Thaidog63
AB0 41968 AF374721 RV108
RV167 RV155
RV860 (CZECH)AF033888 (POL)
U42994 (GER)
U42706 (BOSNIA 1986)RV1189 (N=6, BOSNIA 1986, MONT 1999-2000)
U43008 (SLOV)U42708 (AUS)
U42605 (FRA)U42701 (GER)
U22839 (SCG)
U42713 (BEL)
7270
97
74
0.010.01
CE
13
0.01
RV51 USA fox U22634
U22487 U22640
RV53 RAVN2756
RV1336 RV1420 4055DG
RV303 RV277 RV61 Africa3 U22627
DQ837462 U22484 CVS
Takamen U22637
9126MEX U22651 U22629
DQ 837474 U42705
RV1185 Bulgfox02
RV1194 RV1219
RV1211 RV1153
RV1147 RV1162 RV1151N
U42703 RV1154
DQ837475 RV202
DQ837408 DQ837385
DQ837448 DQ837383
DQ837411 DQ837412
U22480 U22482 DQ837443
U43007 AY277576 AF033879
RV1334 RV259 U42 605
U42701 AF033888 RV860 U42708
U43008 RV1189
U42706 U22839 U42994
U42713 U42998
U43001 RV1197
RV1198 RV1223 RV2028
AF033881 RV1267
RV1308 RV1538
RV1222 RV1268
RV1240 RV1257
RV1251 RV1239 RV1247
RV1243 RV1188 RV905
RV1168 R V1176
RV1157 RV1161 RV1202
RV1207 RV1208 RV1209 RV1255 RV1171
RV1192 RV1266
RV1184 RV1289
RV1287 RV1196 RV1292 RV1339 RV1269
RV1234 RV1272 RV1274 RV1244 RV1230 RV1237 RV1214 RV1227
RV341 RV338
RVU22653 AB178892
Thaidog63 AB0 41968
AF374721 RV108
RV167 RV155
Northern SerbiaDog, cat, fox, deer
1999-2000
Austria Dog 2001 ex Belgrade
Northern SerbiaDog, cat, fox1977-2001
U42998U43001
RV1197RV1198
RV1223RV2028
AF033881RV1267
RV1308RV1538
RV1222RV1268
RV1240RV1257
RV1251RV1239RV1247
RV1243RV1188
RV905RV1168
RV1176RV1157
RV1161RV1202
RV1207RV1208
RV1209RV1255
RV1171RV1192
RV1266RV1184
RV1289RV1287
RV1196RV1292RV1339
RV1269RV1234
RV1272RV1274RV1244RV1230RV1237RV1214RV1227
Hungary, Bosnia, Serbia,Montenegro, Poland, Czech Rep
Dog, cat, fox, horse, cattle1986-2006
73
70
0.01
RV51 USA fox U22634
U22487 U22640
RV53 RAVN2756
RV1336 RV1420 4055DG
RV303 RV277 RV61 Africa3 U22627
DQ837462 U22484 CVS
Takamen U22637
9126MEX U22651 U22629
DQ 837474 U42705
RV1185 Bulgfox02
RV1194 RV1219
RV1211 RV1153
RV1147 RV1162 RV1151N
U42703 RV1154
DQ837475 RV202
DQ837408 DQ837385
DQ837448 DQ837383
DQ837411 DQ837412
U22480 U22482 DQ837443
U43007 AY277576 AF033879
RV1334 RV259 U42 605
U42701 AF033888 RV860 U42708
U43008 RV1189
U42706 U22839 U42994
U42713 U42998
U43001 RV1197
RV1198 RV1223 RV2028
AF033881 RV1267
RV1308 RV1538
RV1222 RV1268
RV1240 RV1257
RV1251 RV1239 RV1247
RV1243 RV1188 RV905
RV1168 R V1176
RV1157 RV1161 RV1202
RV1207 RV1208 RV1209 RV1255 RV1171
RV1192 RV1266
RV1184 RV1289
RV1287 RV1196 RV1292 RV1339 RV1269
RV1234 RV1272 RV1274 RV1244 RV1230 RV1237 RV1214 RV1227
RV341 RV338
RVU22653 AB178892
Thaidog63 AB0 41968
AF374721 RV108
RV167 RV155
Northern SerbiaDog, cat, fox, deer
1999-2000
Austria Dog 2001 ex Belgrade
Northern SerbiaDog, cat, fox1977-2001
U42998U43001
RV1197RV1198
RV1223RV2028
AF033881RV1267
RV1308RV1538
RV1222RV1268
RV1240RV1257
RV1251RV1239RV1247
RV1243RV1188
RV905RV1168
RV1176RV1157
RV1161RV1202
RV1207RV1208
RV1209RV1255
RV1171RV1192
RV1266RV1184
RV1289RV1287
RV1196RV1292RV1339
RV1269RV1234
RV1272RV1274RV1244RV1230RV1237RV1214RV1227
Hungary, Bosnia, Serbia,Montenegro, Poland, Czech Rep
Dog, cat, fox, horse, cattle1986-2006
73
70
Isolates belonging to EE Group
14
Isolates Closest to Middle East Group
0.01
RV51 USA fox U22634
U22487 U22640
RV53 RAVN2756
RV1336 RV1420
4055DG RV303
RV277 RV61 Africa3 U22627 DQ837462
U22484 CVS
Takamen U22637
9126MEX U22651
U22629 DQ 837474
U42705 RV1185
Bulgfox02 RV1194 RV1219 RV1211
RV1153 RV1147 RV1162 RV1151N U42703 RV1154 DQ837475
RV202 DQ837408
DQ837385 DQ837448 DQ837383
DQ837411 DQ837412
U22480 U22482
DQ837443 U43007 AY277576
AF033879 RV1334 RV259
U42 605 U42701 AF033888
RV860 U42708
U43008 RV1189
U42706 U22839 U42994
U42713 U42998
U43001 RV1197 RV1198 RV1223 RV2028 AF033881
RV1267 RV1308
RV1538 RV1222
RV1268 RV1240
RV1257 RV1251
RV1239 RV1247 RV1243
RV1188 RV905 RV1168 R V1176 RV1157
RV1161 RV1202
RV1207 RV1208
RV1209 RV1255
RV1171 RV1192
RV1266 RV1184
RV1289 RV1287
RV1196 RV1292 RV1339 RV1269
RV1234 RV1272 RV1274 RV1244 RV1230 RV1237 RV1214 RV1227
RV341 RV338
RVU22653 AB178892
Thaidog63 AB0 41968 AF374721 RV108
RV167 RV155
U22629DQ 837474
U42705 SCG Cow 1981RV1185 Montenegro Cow 1978
Bulgaria fox 2002RV1194
RV1219RV1211
RV1153RV1147
RV1162RV1151N
U42703RV1154
DQ837475RV202
DQ837408DQ837385
DQ837448DQ837383
DQ837411DQ837412
U22480U22482
DQ837443
0.01
FRY
Serbia fox
Turkey 2
Israel
Turkey 1
0.01
RV51 USA fox U22634
U22487 U22640
RV53 RAVN2756
RV1336 RV1420
4055DG RV303
RV277 RV61 Africa3 U22627 DQ837462
U22484 CVS
Takamen U22637
9126MEX U22651
U22629 DQ 837474
U42705 RV1185
Bulgfox02 RV1194 RV1219 RV1211
RV1153 RV1147 RV1162 RV1151N U42703 RV1154 DQ837475
RV202 DQ837408
DQ837385 DQ837448 DQ837383
DQ837411 DQ837412
U22480 U22482
DQ837443 U43007 AY277576
AF033879 RV1334 RV259
U42 605 U42701 AF033888
RV860 U42708
U43008 RV1189
U42706 U22839 U42994
U42713 U42998
U43001 RV1197 RV1198 RV1223 RV2028 AF033881
RV1267 RV1308
RV1538 RV1222
RV1268 RV1240
RV1257 RV1251
RV1239 RV1247 RV1243
RV1188 RV905 RV1168 R V1176 RV1157
RV1161 RV1202
RV1207 RV1208
RV1209 RV1255
RV1171 RV1192
RV1266 RV1184
RV1289 RV1287
RV1196 RV1292 RV1339 RV1269
RV1234 RV1272 RV1274 RV1244 RV1230 RV1237 RV1214 RV1227
RV341 RV338
RVU22653 AB178892
Thaidog63 AB0 41968 AF374721 RV108
RV167 RV155
U22629DQ 837474
U42705 SCG Cow 1981RV1185 Montenegro Cow 1978
Bulgaria fox 2002RV1194
RV1219RV1211
RV1153RV1147
RV1162RV1151N
U42703RV1154
DQ837475RV202
DQ837408DQ837385
DQ837448DQ837383
DQ837411DQ837412
U22480U22482
DQ837443
0.01
FRY
Serbia fox
Turkey 2
Israel
Turkey 1U22629DQ 837474
U42705 SCG Cow 1981RV1185 Montenegro Cow 1978
Bulgaria fox 2002RV1194
RV1219RV1211
RV1153RV1147
RV1162RV1151N
U42703RV1154
DQ837475RV202
DQ837408DQ837385
DQ837448DQ837383
DQ837411DQ837412
U22480U22482
DQ837443
0.010.01
FRY
Serbia fox
Turkey 2
Israel
Turkey 1
15
Serbian Fox Group
0.01
RV51 USA fox U22634 U22487 U22640
RV53 RAVN2756 RV1336
RV1420 4055DG RV303
RV277 RV61
Africa3 U22627
DQ837462 U22484
CVS Takamen
U22637 9126MEX U22651
U22629 DQ 837474
U42705 RV1185
Bulgfox02 RV1194
RV1219 RV1211
RV1153 RV1147
RV1162 RV1151N U42703 RV1154 DQ837475
RV202 DQ837408 DQ837385
DQ837448 DQ837383 DQ837411
DQ837412 U22480 U22482
DQ837443 U43007 AY277576 AF033879 RV1334
RV259 U42 605
U42701 AF033888
RV860 U42708
U43008 RV1189
U42706 U22839 U42994
U42713 U42998
U43001 RV1197
RV1198 RV1223 RV2028
AF033881 RV1267
RV1308 RV1538
RV1222 RV1268
RV1240 RV1257 RV1251 RV1239 RV1247 RV1243
RV1188 RV905 RV1168 R V1176 RV1157 RV1161 RV1202
RV1207 RV1208
RV1209 RV1255
RV1171 RV1192
RV1266 RV1184
RV1289 RV1287
RV1196 RV1292 RV1339
RV1269 RV1234
RV1272 RV1274 RV1244 RV1230 RV1237 RV1214 RV1227
RV341 RV338 RVU22653
AB178892 Thaidog63 AB0 41968
AF374721 RV108 RV167 RV155
Serbia fox (1986, 1997)
0.01
Bulgfox02RV1194
RV1219RV1211
RV1153RV1147 (n=9)
RV1162 catRV1151
U42703RV1154 (n=3)
77
Serbia fox (1972-1977)
NW Bulgaria fox (2002)
0.01
RV51 USA fox U22634 U22487 U22640
RV53 RAVN2756 RV1336
RV1420 4055DG RV303
RV277 RV61
Africa3 U22627
DQ837462 U22484
CVS Takamen
U22637 9126MEX U22651
U22629 DQ 837474
U42705 RV1185
Bulgfox02 RV1194
RV1219 RV1211
RV1153 RV1147
RV1162 RV1151N U42703 RV1154 DQ837475
RV202 DQ837408 DQ837385
DQ837448 DQ837383 DQ837411
DQ837412 U22480 U22482
DQ837443 U43007 AY277576 AF033879 RV1334
RV259 U42 605
U42701 AF033888
RV860 U42708
U43008 RV1189
U42706 U22839 U42994
U42713 U42998
U43001 RV1197
RV1198 RV1223 RV2028
AF033881 RV1267
RV1308 RV1538
RV1222 RV1268
RV1240 RV1257 RV1251 RV1239 RV1247 RV1243
RV1188 RV905 RV1168 R V1176 RV1157 RV1161 RV1202
RV1207 RV1208
RV1209 RV1255
RV1171 RV1192
RV1266 RV1184
RV1289 RV1287
RV1196 RV1292 RV1339
RV1269 RV1234
RV1272 RV1274 RV1244 RV1230 RV1237 RV1214 RV1227
RV341 RV338 RVU22653
AB178892 Thaidog63 AB0 41968
AF374721 RV108 RV167 RV155
Serbia fox (1986, 1997)
0.01
Bulgfox02RV1194
RV1219RV1211
RV1153RV1147 (n=9)
RV1162 catRV1151
U42703RV1154 (n=3)
77
Serbia fox (1972-1977)
NW Bulgaria fox (2002)Serbia fox (1986, 1997)
0.01
Bulgfox02RV1194
RV1219RV1211
RV1153RV1147 (n=9)
RV1162 catRV1151
U42703RV1154 (n=3)
77
Serbia fox (1972-1977)
NW Bulgaria fox (2002)
100
16
Conclusions Evidence for a number of concurrent independent rabies cycles
Supports previously published antigenic typing data (Stankov et al - mAbs)
All FRY isolates within Cosmopolitan lineage
Relationships - geographical, host species and chronological
Potential for presence intermediate viruses (fox-dog- cat)
Additional panel currently being analysed (2001-2006 isolates) – assess survival of viral variants
Future work will involve evolutionary clock analysis (Full N & G gene sequencing underway)
17
Conclusions (2)
Royal Society Travel Grants – Russian & Chinese Programmes Sharing of isolates / collaborative studies Exchange visits Targeted surveillance programmes Russian Collaborative Programmes (VLA/Dr Botvinkin & Dr Kuzmin)
publications: Kuzmin et al 2004, Botvinkin et al 2006, Mansfield et al 2006 Chinese Collaborative Programme (VLA/ Prof Tu & Dr Jiang)
Canine RABV sequences analysed Active/passive targetted bat surveillance (Prof Tu to present data)
18
Prof A. Fooks, Dr L McElhinneyD. Marston, Dr N Johnson, C Black
Dr N Tordo (France)
Prof Tu , Dr Jiang (China)
Defra grant SE0420Royal Society
Dr S Stankov (Serbia & Montenegro)
Dr T Muller (Germany)
Acknowledgements