STUDY DEMOSTRATES EFFECTIVENESS OF

COMBINATION THERAPY FOR BREAST CANCER

CELLS IN VITROAND

SAFER ALTERNATIVE TO EXPERIMENTAL GENE

THERAPY AGAINST HIV INFECTION

Steven Olaya Ramírez

Medical Student

July 23, 2012

INTRODUCTIONA

combination of some

treatments can be the

key for found the cure

Safer methods are perhaps the

more effectives

A study conducted at Boston University School of Medicine (BUSM) demonstrates an effective combination therapy for breast cancer cells in vitro.

News MedicalJuly 3, 2012

STUDY DEMOSTRATES EFFECTIVENESS OF COMBINATION

THERAPY FOR BREAST CANCER CELLS IN VITRO

STUDY DEMOSTRATES EFFECTIVENESS OF COMBINATION THERAPY FOR BREAST

CANCER CELLS IN VITRO

New therapy testead In Vitro

A combination of an epigenetic drug with a protease inhibitor on breast cancer cell lines

That are hormone responsive and breast cancer lines, like triple negative, that are not hormone responsive

STUDY DEMOSTRATES EFFECTIVENESS OF COMBINATION THERAPY FOR BREAST

CANCER CELLS IN VITRO

Histone deacetylases

inhibitors (HDACi) and

calpeptin

Inhibits calpain, a protein

involved in the regulation of

signaling proteins

STUDY DEMOSTRATES EFFECTIVENESS OF COMBINATION THERAPY FOR BREAST

CANCER CELLS IN VITRO

HDCAi CALPEPTIN

Inhibited cell growth and

increased cell death in both

cancer cell lines

OBSERVATION

I think that last study is so important for keep moving forward in the search of a treatment for the breast cancer that is so devastating for the female population. In the article says that cancer is like a car without brakes, so if this therapy stop the car with HDACi as brakes for the cell growth, it could be a brilliant discover that deserves attention and a good investment; maybe others researchers would join the research.  

SAFER ALTERNATIVE TO EXPERIMENTAL GENE THERAPY

AGAINST HIV INFECTION

Scientists at The Scripps Research Institute have discovered a surprisingly simple and safe method to disrupt specific genes within cells. The scientists highlighted the medical potential of the new technique by demonstrating its use as a safer alternative to an experimental gene therapy against HIV infection.

News MedicalJuly 2, 2012

SAFER ALTERNATIVE TO EXPERIMENTAL GENE THERAPY AGAINST HIV INFECTION

New technique employs zinc finger

nuclease (ZFN) proteins

It can bind and cut DNA at precisely defined locations

in the genome

ZFN in DNA

SAFER ALTERNATIVE TO EXPERIMENTAL GENE THERAPY AGAINST HIV INFECTION

ZFN proteins cannot cross cell

membranes

So the standard ZFN delivery method has been a gene-therapy technique employing a relatively harmless

virus to carry a designer ZFN gene

into cells

Inside, ZFN gene starts producing ZFN proteins, which seek and destroy their

target gene

SAFER ALTERNATIVE TO EXPERIMENTAL GENE THERAPY AGAINST HIV INFECTION

Virus delivery method has two

basic risks

Viral DNA may end up being

incorporated randomly into cellular DNA,

disrupting a valuable gene such as a

tumor-suppressor gene

ZFN genes could end up producing too

many ZFN proteins, resulting in a high

number of "off-target" DNA cuts

OBSERVATION

My opinion is that ZFN proteins are a very interesting alternative for the treatment of certain diseases that can be worked with gene therapy, also presents itself as a safer option and accurate than other types of gene therapy, which deserves to be taken into account in certain researches. And although this method has a transport risk (viral transport), also has another points that makes this therapy safest than others. 

MEDICAL UTILITY STUDY DEMOSTRATES

EFFECTIVENESS OF COMBINATION THERAPY FOR BREAST CANCER

CELLS IN VITRO

The use of combination therapies that can be prescribed to a patient for treatment of their disease, in this specific case of breast cancer.

MEDICAL UTILITY STUDY DEMOSTRATES

EFFECTIVENESS OF COMBINATION THERAPY FOR BREAST CANCER

CELLS IN VITRO

If the combination of therapies is successful, it creates the possibility to attack breast cancer and prevent more women continue to die around the world.

MEDICAL UTILITY SAFER ALTERNATIVE TO

EXPERIMENTAL GENE THERAPY AGAINST HIV INFECTION

With the techniques of gene therapy can treat certain diseases (in this specific case of HIV infection) and also prevent others may appear in the course of life, DNA modification opens new doors to medicine.

MEDICAL UTILITY SAFER ALTERNATIVE TO

EXPERIMENTAL GENE THERAPY AGAINST HIV INFECTION

HIV is in parts of the world an epidemic, for example in parts of africa, this new therapy that comes out gives new hope to combat this virus that has claimed the lives of many people on the planet.

BIBLIOGRAPHY

Study demonstrates effectiveness of combination therapy for breast cancer cells in vitro. News medical .(July 3, 2012)

Safer alternative to experimental gene therapy against HIV infection. News medical. (July 2,2012)

Martínez S, Lina María. Biología Molecular. 7 ed Medellín: UPB. Fac Medicina.