Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing...
-
date post
19-Dec-2015 -
Category
Documents
-
view
217 -
download
2
Transcript of Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing...
![Page 1: Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649d2b5503460f949fff20/html5/thumbnails/1.jpg)
Modelling Cell AttachmentJonathan A. Ratcliffe
![Page 2: Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649d2b5503460f949fff20/html5/thumbnails/2.jpg)
Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work
![Page 3: Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649d2b5503460f949fff20/html5/thumbnails/3.jpg)
Aims To visualise a cell as it undergoes the
transition from attaching and spreading to crawling
To create an accurate three-dimensional representation of the cell so that predictions can be made about spreading using the dynamic contact line of the cell.
![Page 4: Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649d2b5503460f949fff20/html5/thumbnails/4.jpg)
The dynamic contact angle
![Page 5: Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649d2b5503460f949fff20/html5/thumbnails/5.jpg)
2 stages of cell spreading: Passive and Active
![Page 6: Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649d2b5503460f949fff20/html5/thumbnails/6.jpg)
Theory
J. Oliver, et al. Thin-film theories for two-phase reactive flow models of active cell motion
Mathematical Medicine and Biology (2005) 22, 53–98
Dynamic Contact angle
(to be determined)
Static contact angle
Network density at contact line
Mass transfer rate from G- to F- actin at contact line
Outward normal velocity for contact line.
Fluid forces Motile machinery
![Page 7: Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649d2b5503460f949fff20/html5/thumbnails/7.jpg)
Imaging LavaCell™ stain chosen as: Cost effective Simple to use, add 1.8μl for 1.5ml media Long stokes shift (fluorescence lifetime) No cytotoxicity observed up to 24h. Best images
![Page 8: Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649d2b5503460f949fff20/html5/thumbnails/8.jpg)
![Page 9: Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649d2b5503460f949fff20/html5/thumbnails/9.jpg)
Image Processing
![Page 10: Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649d2b5503460f949fff20/html5/thumbnails/10.jpg)
Image Prossesing
![Page 11: Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649d2b5503460f949fff20/html5/thumbnails/11.jpg)
![Page 12: Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649d2b5503460f949fff20/html5/thumbnails/12.jpg)
![Page 13: Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649d2b5503460f949fff20/html5/thumbnails/13.jpg)
![Page 14: Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649d2b5503460f949fff20/html5/thumbnails/14.jpg)
Cell imaged at 20 minutes after seeding.
![Page 15: Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649d2b5503460f949fff20/html5/thumbnails/15.jpg)
Cell imaged 60 minutes after seeding.
![Page 16: Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649d2b5503460f949fff20/html5/thumbnails/16.jpg)
Human Osteosarcoma cell 70 minutes after seeding.
![Page 17: Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649d2b5503460f949fff20/html5/thumbnails/17.jpg)
Conclusions
Image processing technique accurately finds outline of cell segments.
3D representations visually similar to SEM images of fixed cells.
![Page 18: Modelling Cell Attachment Jonathan A. Ratcliffe Outline Aims Theory Imaging Image Processing Comparison to SEM Conclusions Future work.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649d2b5503460f949fff20/html5/thumbnails/18.jpg)
Future Work To look at transparent coated surfaces
such as: TiN, TiO2, Gold, SS 316L. Patterned surfaces Surface topography using embossed glass