MLAB 1415: Hematology

Micros 60

Micros 60• 3-part differential• Sample size= 10µL• Environment

– Indoor only– Level work station– Avoid direct exposure to sunlight– Proper ventilation– 60-90oF

• Linearity (AMR)– WBC (103/mm3): 0-100– RBC (106/mm3): 0-8.0– Hgb (g/dL): 2.0-27– Hct (%): 1.8-82.3– Plt (103/mm3): 25-4990

Parameters

Parameters• WBC• RBC• Hgb• Hct• MCV• MCH• MCHC• RDW• Platelet• MPV• Lym #• Lym%• Mono #• Mono%• Gra#• Gra%

Measurements/Calculations• WBC/RBC/PLT

– Impedance change• HGB

– Spectrophotometry• LYM%/MON%/GRA%

– Impedance change

Calculations• MCV,

MCH,MCHC,RDW,MPV,LYM#, MON#, GRA#

Stats

Principle of operation: The counting principle used for the red blood cells, white blood cells and platelets is based on the variations in impedance generated by the passage of cells through a calibrated aperture.

Number of samples which can be analyzed per hour: 60

Number of tests performed on this analyzer: 1

Quality control: Three levels run each day of patient testing, or following instrument repair, reagent lot change, or when indicated.

Calibration: Shift in control values, replacement of instrument parts, reagent number change, or when indicated

Preventative maintenance: Daily: start up and shut down, general cleaning

Acceptable samples: whole blood from an EDTA tube ran within 4 hours of collection

Limitations: Lipemia, cold agglutinins, high WBC’s

Equipement

• 8 Main parts– Electrical supply– Electronic board– Dilution pneumatics– Control panel with

keyboard and LCD screen– Reagent compartment– Printer– Smart card reader– Bar code reader (optional)

Reagents

– Diluent• ABX minidil: sodium hydroxide/azide• Determines and differenciates blood cells

– Cleaner• ABX miniclean: enzymatic detergent• Cleans system

– LYSE: potassium cyanide• ABX Alphalyse: Lyses RBCs, releasing hemoglobin and

WBCs. WBCs are counted by impedance.

• Waste

Distribution curves

• WBC• RBC• Plt

Daily Start Up1. Turn on instrument, once on, the start-up will begin automatically

1. Instrument is performing a cleaning and rinsing cycle: detergent in the chamber is rinsed with diluent

2. Cycle lasts about 130 seconds2. At cycle completion, background values will be displayed. These

values must be less than the below:1. WBC < 0.3 x 103/mm3

2. RBC < 0.02 x 106/mm3

3. Hgb < 0.0 g/dL4. Plt < 10x 103/ mm3

5. If the values are not within this range, another cycle will begin. If still not in range, the Micros will do another start-up. After 3 failed cycles, it will give a “start-up failed” message. Troubleshooting will now start.

Daily start-up con’t

3. Run a primer sample4. Perform QC and evaluate5. Complete maintenance sheet6. If all QC in, can continue with patient

samples.

Running a Patient

1. Press the ID button on the key pad

2. Manually enter the ID. Use the up and down arrows to enter alphabetical characters. Please Enter after each entry.

Shutdown

1. Hit the Stand by key, wait 1 minute

2. Switch off instrument and printer.