Mireia Massanell, Commercial Manager MICRODAY 29 & 31 May, … QFast Platform... · 2019-06-07 ·...
Transcript of Mireia Massanell, Commercial Manager MICRODAY 29 & 31 May, … QFast Platform... · 2019-06-07 ·...
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Integrated analytical platformMakes food safety testing simpler
Mireia Massanell, Commercial ManagerMICRODAY 29 & 31 May, Italy
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Background
Flexible bioanalytical platform
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Enzymatic assays ImmunoassaysDNAassays
Chromogenic PlatesBiochemical Galleries
ELISAImmunostrips (LFD)
PCRGenotyping
Background – Current technologies
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Enzymatic assays ImmunoassaysDNAassays
Background
Colorimetry AbsorbanceFluorimetry
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Electrochemical Read Out platform
Enzymatic assays ImmunoassaysDNAassays
Background
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Background
Electrochemical Read Out Platform
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Analytical Assay• ELISA/ELONA : Indirect,
Competition, Sandwich• Minimum manipulation
Magnetic beads modification• Immuno: Antibodies & Fab•DNA &RNA probes.• Protein Antigen & Markers
Bacterial determinations• Enzymatic substrates• Enrichment media• Selective growth media
Technology Basis
Electrochemical signal transduction as final step of customised design of assay formats, capturing technique and selective reagents”
ODM, OEM services for companies interested in developing their own detection kits
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Integrated Analytical Platform
Electrochemical Read Out platform
bact-QFast
ELISA/ELONA
PCR
gen-QFast
immuno-QFast
Salmonella sppE. coliCronobacter Sakazakii
Salmonella sppLegionella spp
Gluten, Soya, Milk, Egg proteinsMicotoxins
L. monocytogenesS. enteritidisS. typhimuriumCampylobacter
Mycoplasma G.
24h assays < 1 CFU/samplePOSITIVE/NEGATIVE resultsNo need for interpretation
No washing, colourrevealing or stop solution
No sample turbidity orinterferences fromcomplex matrixes
POSITIVE/NEGATIVE resultsNo software associatedNo need forinterpretation
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Incubators - Mixers
Instrumentation pack
Enzymatic Assays▪ New integral solution to analytical assays.
▪ Low cost equipment.Laboratory use and in fieldapplications
▪ No maintenance service orcalibration required. Heavy duty design.
▪ From unitary to high samplethroughput
▪ Custom made solutions to different applications
Racks - Magnets
Biosensors
Electrochemical readers
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Integrated Analytical Platform
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bact-
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Bacteria Selective Detection
3 steps of Selectivity: Enrichment + Capture + Read out
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Salmonella in water: Selective and Fast Response
Salmonella Detection in water
Ease-of-use: 3 droplets
No agar platesCulture plate confirmation OK
x 8 samples
Customised to sample type:
Water: Media 1 BPW/SOFTSludge, Soil : Media 1 HARD
Customised to client:
Media ready-to-useMedia dehidratedDry-Bags
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Salmonella Detection in water
Salmonella in water: Advantageous vs. ISO 19250
BPW (18 ± 2 h)36 ± 2 °C
Agar XLD 24±3 h36 ± 2 °C
Agar Chromo24±3 h37 ± 1 °C
Media 1 (16 ± 1 h)37 °C
10 mL RVS / MKTTn24±3 → 48±4 h
41,5 ± 1 °C
Media 2 (7 ± 1 h)41,5 °C
Monday 17:00
Tuesday 16:00
Thursday 10:00
Friday 10:00
Result
Result
- From 1,5 to 2,5 daysfaster
- Mininum manipulation
- Minimum consumables
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Salmonella in water: Advantageous vs. Membrane + XLD
Membrane into XLD (24 ± 3 h)36 ± 2 °C
Membrane into Media 1 (16 ± 1 h)
37 °C
Media 2 (7 ± 1 h)41,5 °C
Monday 17:00
Tuesday 16:00 Result
100 % exclusivity
Tuesday 17:00
Result
Red colonies most with black centers (H2S)Salmonella spp
Reported Bacteria XLD false positives:Pseudomona sppShigellaChronobacteriumProteus
Soil & Sludges includedNevermind the accompanying flora
Salmonella Detection in water
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Pathogen Solutions
Whole portfolio for pathogen bacteria detection with
ISO 16140 Certification as an International Quality Standard
Salmonella food & veterinary Cronobacter sakazakii Escherichia Coli
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Platform that grows
New QFast®-Easy8 Platform for 96 sample processing/day
Available for QFast®- EASY Protocols (Food and Raw Material)
8-samples read out in 60 s
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gen-
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DNA detection & genotyping
3 Steps of Selectivity: Enrichment + PCR + Read out
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DNA safety solutions
Salmonella enteritidis, typhimurium, sppListeria monocytogenes
Whole portfolio for pathogen bacteria detection with
ISO 16140 Certification as an International Quality Standard
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DNA safety solutions
Salmonella enteritidis, typhimurium, sppListeria monocytogenes
▪ New protocols under development
ISO 16149 Certified 2019
- New presentation in
Multiwell plate under development
- Easy to manage volume
transfers with multichannel micropipette (8 by 8)
- Read out by 8 samples in 1min until 32 samples.
- Internal Control for industry and labs to genotype Salmonella strains in less than 3h.
- New presentation in Multiwellplate under development
- Reduced times and steps in protocol !
- Read out by 8 samples in 1min until 32 samples.
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immuno-
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XXXXXELISA to “ELIMSA”
• Plate-adsorption bio-affinity element
• Read out through optical techniques:
• Challenges• Maximize selectivity
• Decrease background signals
• Magnetic bead as substrates
• Read out through electrochemical techniques
• Advantages• Improved selectivity
• Improved background signals
• Improved sensitivity
“ELIMSA” : Electrochemical immunomagnetic
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Electrochemical assay
Electrochemical Assay : Easily matching ELISA performance
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Electrochemical Assay vs. ELISA: Legionella detection commercial kit
Real case
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Flexible throughput
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✓ Expanding platform✓ New areas to be covered✓ Reliable✓ Certified
bact-QFast
gen-QFast
immuno-QFast