Mindray BS 300 Analizor Service Manual

8/20/2019 Mindray BS 300 Analizor Service Manual

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BS-300

Chemistry Analyzer

Service Manual



I

© 2006 Shenzhen Mindray Bio-medical Electronics Co., Ltd. All rights Reserved.

For this Service Manual, the issued Date is 2006-06 (Version: 1.0).

Intellectual Property Statement

SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS CO., LTD. (hereinafter calledMindray) owns the intellectual property rights to this Mindray product and thismanual. This manual may refer to information protected by copyrights or patents anddoes not convey any license under the patent rights of Mindray, nor the rights ofothers. Mindray does not assume any liability arising out of any infringements ofpatents or other rights of third parties.

Mindray intends to maintain the contents of this manual as confidential information.Disclosure of the information in this manual in any manner whatsoever without the

written permission of Mindray is strictly forbidden.

Release, amendment, reproduction, distribution, rent, adaption and translation of thismanual in any manner whatsoever without the written permission of Mindray isstrictly forbidden.

, , , , are the registeredtrademarks or trademarks owned by Mindray in China and other countries. Allother trademarks that appear in this manual are used only for editorial purposeswithout the intention of improperly using them. They are the property of theirrespective owners.

Responsibility on the Manufacturer Party

Contents of this manual are subject to changes without prior notice.

All information contained in this manual is believed to be correct. Mindray shall notbe liable for errors contained herein nor for incidental or consequential damages inconnection with the furnishing, performance, or use of this manual.

Mindray is responsible for safety, reliability and performance of this product only inthe condition that:

all installation operations, expansions, changes, modifications and repairs of thisproduct are conducted by Mindray authorized personnel;

the electrical installation of the relevant room complies with the applicable

national and local requirements; the product is used in accordance with the instructions for use.

WARNING:

It is important for the hospital or organization that employs thisequipment to carry out a reasonable service/maintenance plan.Neglect of this may result in machine breakdown or injury of humanhealth.

NOTE:

This equipment is to be operated only by medical professionals trainedand authorized by Mindray or Mindray-authorized distributors.



II

Warranty

THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES,EXPRESSED OR IMPLIED, INCLUDING WARRANTIES OF MERCHANTABILITYOR FITNESS FOR ANY PARTICULAR PURPOSE.

Exemptions

Mindray's obligation or liability under this warranty does not include anytransportation or other charges or liability for direct, indirect or consequentialdamages or delay resulting from the improper use or application of the product or theuse of parts or accessories not approved by Mindray or repairs by people other thanMindray authorized personnel.

This warranty shall not extend to:

any Mindray product which has been subjected to misuse, negligence oraccident;

any Mindray product from which Mindray's original serial number tag or productidentification markings have been altered or removed;

any product of any other manufacturer.

Return Policy

Return Procedure

In the event that it becomes necessary to return this product or part of this product toMindray, the following procedure should be followed:

1 Obtain return authorization: Contact the Mindray Service Department andobtain a Customer Service Authorization (Mindray) number. The Mindraynumber must appear on the outside of the shipping container. Returnedshipments will not be accepted if the Mindray number is not clearly visible.Please provide the model number, serial number, and a brief description ofthe reason for return.

2 Freight policy: The customer is responsible for freight charges when thisproduct is shipped to Mindray for service (this includes customs charges).

3 Return address: Please send the part(s) or equipment to the address offeredby Customer Service department

Company Contact

Manufacture: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.

Address: Mindray Building, Keji 12th Road South, Hi-tech Industrial Park,

Nanshan, Shenzhen, P.R.China, 518057Phone: +86 755 26582479 26582888

Fax: +86 755 26582500 26582501



i

Preface

Who Should Read This Manual

This manual is written for service professionals authorized by Mindray.

Conventions Used in This Manual

Safety Symbols

This chart explains the symbols used in this manual.

When you see … Then …

WARNING: Read the statement following the symbol. Thestatement is alerting you to an operating hazardthat can cause personal injury.

BIOHAZARD:Read the statement following the symbol. Thestatement is alerting you to a potentiallybiohazardous condition.

CAUTION: Read the statement following the symbol. Thestatement is alerting you to a possibility ofsystem damage or unreliable results.

NOTE: Read the statement following the symbol. Thestatement is alerting you to information thatrequires your attention.

Graphics

All graphics, including screens and printout, are for illustration purpose only andmust not be used for any other purposes.



Contents

1

Contents

Preface........................................................................................................................................... i

Who Should Read This Manual .............................................................................................. i

Conventions Used in This Manual .......................................................................................... i

1 Specifications....................................................................................................................1-1

1.1 System Feature ...................................................................................................1-1

1.2 Loading System Feature .....................................................................................1-1

1.3 Analysis System Feature.....................................................................................1-2

1.4 Others..................................................................................................................1-2

2 System Installation ...........................................................................................................2-1

2.1 Check before Installation.....................................................................................2-1

2.2

Installation Procedure..........................................................................................2-1

3 System Descriptions........................................................................................................3-1

3.1 Dispensing System..............................................................................................3-1

3.1.1 Probe assemblies.................................................................................3-1

3.1.2 Disk assemblies ...................................................................................3-2

3.2 Feeder .................................................................................................................3-3

3.2.1 Feeder assemblies...............................................................................3-3

3.2.2 Manipulator...........................................................................................3-4

3.3 Temperature Control System...............................................................................3-5

3.3.1 Temperature control assembly .............................................................3-5

3.3.2 Reagent preheating..............................................................................3-6

3.3.3 Reagent refrigeration............................................................................3-7

3.4

Photometric System ............................................................................................3-8

3.5 Fluid System........................................................................................................3-8

3.6 ISE Module (optional)........................................................................................3-10

4 Functions of Boards.........................................................................................................4-1

4.1 Main Control Board..............................................................................................4-1

4.2 Power Drive Board ..............................................................................................4-2

4.3 A/D Conversion Board.........................................................................................4-2

4.4 Reagent Refrigeration Board...............................................................................4-2

4.5 Level Detection Boards .......................................................................................4-3

4.6 Feeder Connection Board ...................................................................................4-3

4.7 Manipulator Connection Board............................................................................4-3

4.8

Probes Connection Board ...................................................................................4-4 4.9 Power Supply Assembly ......................................................................................4-4

5 Maintenance and Service.................................................................................................5-1

5.1 Replacing Light Filter Assembly ..........................................................................5-1

5.2 Replacing Optical Fiber .......................................................................................5-4

5.3 Adjusting Reaction Disk, Manipulator and Feeder..............................................5-7

5.4 Adjusting Probes and Disks.................................................................................5-8

5.5 Replacing Components of ISE Unit (optional)...................................................5-10

5.5.1 Replacing Tubing................................................................................5-10

5.5.2 Replacing Pumps ...............................................................................5-13

5.5.3 Replacing ISE Module........................................................................5-13

6 Software Introduction.......................................................................................................6-1



Contents

2

6.1 System Software..................................................................................................6-1

6.1.1 System initialization..............................................................................6-1

6.1.2 Shutdown processing...........................................................................6-2

6.2 Control Software..................................................................................................6-2

7 Service Flow......................................................................................................................7-1

7.1

Fluid Level Detection Failure of Reagent Probe..................................................7-1

7.2 Fluid Level Detection Failure of Sample Probe................................................... 7-2

7.3 Liquid Dropping From Probes..............................................................................7-3

7.4 Failing to Detect Level of Water for Washing Exteriors .......................................7-4

7.5 Abnormal Results ................................................................................................7-5

7.5.1 All Results Being Abnormal.................................................................. 7-5

7.5.2 Some Results Being Abnormal.............................................................7-5

7.5.3 Several Results Being Abnormal..........................................................7-6

7.6 Insufficient Light Intensity of Lamp ......................................................................7-7

7.7 Temperature Control Failure................................................................................ 7-8

7.8 Bar Code Scanner (optional) Failure................................................................... 7-9

7.9 Feeder Failure ................................................................................................... 7-11

7.9.1 Transducer Distribution of the Feeder................................................ 7-11

7.9.2

Feeder Failure....................................................................................7-12

7.9.3 Manipulator Failure.............................................................................7-13

7.10 Troubleshooting of ISE Unit (optional)...............................................................7-13

8 Mechanical Structure .......................................................................................................8-1

9 Tools and Parts .................................................................................................................9-1

9.1 Service Tools .......................................................................................................9-1

9.2 Parts ....................................................................................................................9-1

10 Maintenance And Test Software....................................................................................10-1

10.1

General..............................................................................................................10-110.2 Command..........................................................................................................10-3

10.2.1 Single Command Area .......................................................................10-3

10.2.2 Macroinstruction Area.......................................................................10-10

10.3 PARA and Speed.............................................................................................10-13

10.4 Temperature.....................................................................................................10-19

10.5 Photoelectric....................................................................................................10-20

Appendix A Boards Connection Diagrams .............................................................................. A-1

Appendix B Test Points of Boards ............................................................................................ B-1



Specifications

1-1-1

1 Specifications

1.1 System Feature

Dimension: 980mm × 710mm × 1200mm (W × D × H)

Weight: 175kg

Power supply: AC100-130V ± 10% or 200-240V ± 10%

Input power: 1000VA

System: optional, multi-channel, multi-test

Scope: Clinical chemistries

Test types: end-point, kinetic and fixed-time. All support double-reagent and

double-wavelength Calibration type: Linear (single-point, two-point and multi-point), Logistic 4P,

Logistic 5P, Exponential 5P, Polynominal 5P, Parabola, Spline

QC rules: Westgard multi-rule,

−

X -R, Cumulative sum check, Cumulative error,Twin-plot

Tests analyzed simultaneously: 48 (single-reagent) / 24 (double-reagent); if theISE unit (optional) is connected, 3 (Na, K and Cl included) or 4 (Na, K, Cl and Liincluded) tests are added

Throughput: maximum 300tests/h; if the ISE (optional) is connected, maximum420tests/h (Na, K and Cl included) or maximum 480 tests/h (Na, K, Cl and Liincluded)

1.2 Loading System Feature

Sample volume: 3µl~45µl; Precision: 0.5µl; for the ISE (optional), 70µl serum,70µl plasma, 140µl diluted urine

Sample disk: general sample disk, including the inner circle and the outer circle

Sample tube position: 60 positions, including 6 calibrator positions, 3 controlpositions, 5 for STAT sample positions; 5 virtual disks for maximum 300 samples

Sample probe: with a built-in level detector; equipped with auto safeguard;capable of tracking sample level

Washing function: automatically washing interior and exterior of sample probe;carryover no more than 0.1%

Pre-dilution: 4 ≤ dilution rate ≤ 150, taking reaction cuvettes as the container

Reagent volume: 30-450ul; Precision: 1ul

Reagent disk: including the inner circle and the outer circle

Reagent position number: 25/50 reagent positions. Each reagent position isavailable for containing one Hitachi 7060 bottle, one Hitachi 7170 bottle, oneMindray inner-circle bottle or one Mindray outer-circle bottle.

Reagent probe: One independent probe which has a built-in level detector; isequipped with auto safeguard and capable of tracking reagent level

Washing function: automatically washing interiors and exteriors of reagent probes;carryover no more than 0.1%



Specifications

1-2

Mixing bar: for single-reagent tests, it functions after sample dispensing; fordouble-reagent tests, it functions after the dispensing of the sample and thesecond reagent.

1.3 Analysis System Feature

Lamp house: 50w lamp

Light splitting mode: Splitting by optical fiber, filtering by an interference filter.

Half band-width: 10±2nm

Wavelength: 340, 405, 450, 510, 546, 578, 630, 670, 700nm

Absorbance range: -0.1~5, 10mm optical path conversion

Reaction cuvette: 5 × 6 × 25mm, optical path 5mm. Material: PP, disposable.Volume: 750uL

Reaction liquid volume: 180-500µl

Max. reaction time: 20 minutes

Reaction temperature: 37±0.3 with fluctuation of ±0.1

1.4 Others

Operating system: Windows 2000 or Windows XP

Display: optional

System interface: RS-232

Printer: optional

Built-in bar code scanner: optional ISE module: optional



System Installation

2-1

2 System Installation

NOTE:The analyzer should be installed or moved to another place byMindray-authorized personnel only.

2.1 Check before Installation

The user should provide the environment that meets the requirements mentioned inthe Operation Manual. Check if the environment meets the requirements beforeinstalling the analyzer. Refer to the chapter 2 of the Operation Manual for details.

2.2 Installation Procedure

1 Ensure available installation fields and environments in hospitals.

2 Confirm the reagents and calibrators.

3 Go to the installation field, and then check the delivery list for acceptance.

4 Install the four handles on the four angles of the analyzer. Move the analyzerto the installation field, fix the casters, and then remove the handles.

5 Insta ll the computer, display and printer.6 Open the front plate, and check whether cable connections are loose. Open

the top plate, check whether the probe assemblies, reagent disk and sampledisk are intact and in good performance.

7 Connect the communication cable, power cable, grounding wire, waste tankand deionized water tank. Install the used-cuvette bucket, reagent probe,sample probe and mixing bar.

8 Top up the deionized water tank with deionized water.

9 Put reaction cuvettes in the feeder. Remember to check whether the surfacesof the cuvettes are smooth. In case of any bump, remove it before loading thecuvette to the compartment. Do not touch the light transmission part of thecuvette in which the colorimetric reading is taken.

10 Load acid and alkaline detergents to positions 46 and 47, and distilled waterto positions 49 on the reagent disk. Load distilled water to position 60 on thesample disk.

11 Switch on the analyzer as follows: POWER → ANALYZING UNIT POWER→display→ computer → printer.

12 After Windows is started, double-click the icon of BS-300 on the desktop tostart the system software. The system program will automatically finish theself-test, become online and warm up the reaction cuvettes within about 30minutes.

13 Select the [System/Status] menu, and then observe the system status andrecord it in the table below:



System Installation

2-2

Feeder status Unconnected Full Half full Empty

Reaction disktemperature

Reagent disktemperature

Ambient temperature

Waste tank status Abnormal (full) Normal (not full)

Detergent status Abnormal (empty) Normal (available)

Printer status No printer Normal

Main control unit Unconnected Idle Running

Reaction disk unit Unconnected

Reagent disk unit Unconnected

Sample disk unit Unconnected

Loading/unloading unit Unconnected

Temperature controlunit

Unconnected

Lamp

Network connection Unconnected

Wavelength Dark current Light source base

340

405

450

510

546

578

630

670

700

Reference light system

14 Select the [System/Maintenance] menu. Then select the Motion tab page,and implement all sub-steps of each unit to see whether they are normal. Incase of any exception, adjust it.

15 Wash the interiors and exteriors of the sample probe, reagent probe andmixing bar for several times to make the fluid circuit filled.

16 Set parameters for test, reagent and calibration under the Parameters menu.



System Installation

2-3

17 Request for calibration and samples, run and then debug the results.

18 After debugging the results, fill them in the table below:

Test ALT CREA BUN

Target value

2sd range

Test value 1

Test value 2

Test value 3

Test value 4

Test value 5

Test value 6

Test value 7

Test value 8

Test value 9

Test value 10

19 Training

Can the user complete daily tests? Yes No

Is the user familiar with the analytical methods such askinetic, two-point, endpoint?

Yes No

Is the user familiar with the daily, weekly and monthlymaintenance and relevant maintenance methods? Yes No

Is the user skilled in washing dust screens? Yes No

Is the user skilled in cleaning and replacing the probesand the mixing bar?

Yes No

Is the user skilled in replacing the plunger assembliesof syringes?

Yes No

Is the user skilled in replacing the lamp? Yes No

Is the user skilled in maintenance of built-in bar codescanner?

Yes No

Is the user skilled in maintenance of ISE unit? Yes No

Does the user know the positions, roles andpreparation methods of distilled water and acid andalkaline detergents?

Yes No



System Descriptions

3-1

3 System Descriptions

The BS-300 analyzer consists of the analyzing unit, operation unit and output unit.

The analyzing unit consists of the dispensing system, feeder, temperature controlsystem, photometric system and fluid system.

3.1 Dispensing System

The dispensing system consists of the probe assemblies (including the reagentprobe assembly, sample probe assembly and mixing bar assembly), reagent disk,sample disk and reaction disk.

3.1.1 Probe assemblies

Among the probe assemblies, the mixing bar assembly is the same as the reagentprobe assembly and the sample probe assembly, except that the knurled axis is30cm shorter.

Every probe assembly has a horizontal photoelectric switch and a verticalphotoelectric switch. These switches are used for defining horizontal and vertical



System Descriptions

3-2

initial positions of probe assemblies. The horizontal and vertical step motorsprecisely control the horizontal and vertical movements of the probe assemblies, andthe synchronizing belts serve as the gearing.

The shaft and the bushing must corporate with each other precisely, so they cannotbe used confusedly.

3.1.2 Disk assemblies

The three disk assemblies are different in their coders. The coder corresponds to theposition where disks should stop. There is an initial-position mark under every coder.The three coders of the three disks have three coder transducers. Each transducerhas two photoelectric switches for inducing the rotation and initial position of thedisk.

The step motors control the disk assemblies, and the synchronizing belts serve asthe gearing.



System Descriptions

3-3

There is a build-in bar code scanner to the left of the sample disk. The scanner isoptional.

Sample probe assemblyBuild-in bar code scanner

3.2 Feeder

The feeder consists of the feeder assemblies and the manipulator. It is designated tosend cuvette segments to the reaction disk, take out the used ones and abandonthem to the used-cuvette bucket.

3.2.1 Feeder assemblies

The feeder assemblies include the gearing assembly, cuvette compartmentassembly, cuvette-pushing assembly and no-cuvette detection assembly (see thefollowing figure).

The supporting plate of the feeder assemblies is a square piece of steel that isconnected to the analyzing unit by its four poles, which are secured by four nuts. Unscrewing the nuts, you can disassemble the feeder assemblies from the analyzingunit easily.



System Descriptions

3-4

There are five transducers that are shown in the figure below.

The no-cuvette transducer is used to detect whether there is a cuvette segment atthe loading position. The insufficient-cuvette transducer is used for determinewhether there are less than 10 reaction cuvettes in the compartment or not. If yes,the analyzer will give a prompt.

Pressure Transducer

No-Cuvette

Transducer

Cuvette-Pushing

Limit Transducer Insufficient-Cuvette

Transducer

Cuvette-taking limit

transducer

3.2.2 Manipulator

Two step motors (horizontal and vertical) supply power for horizontal and verticalmovements of the manipulator.

The upper finger and lower finger are same in their structures. They work together toreplace used cuvette segments with new ones.

The manipulator runs in a relatively complicated way. There are four transducers onit: vertical transducer, horizontal transducer and two finger transducers.



System Descriptions

3-5

3.3 Temperature Control System

3.3.1 Temperature control assembly

The temperature control assembly of the reaction disk consists of thetemperature-controlled pot, heat-insulating sheath/plate, top heater, bottom heater,reaction disk/cuvettes, photoelectric seat, temperature transducer, fan and controlcircuit.



System Descriptions

3-6

1: Temperature transducer and the support

2: Fan

3: photoelectric seat

4: Heat-insulating sheath5: top heater

6: Cover

7: temperature-controlled pot

8: bottom heater

Upper heater: square in shape, 220/110VAC, 125W

Lower heater: ring in shape, 220/110VAC, 350W

Total power: 475W.

The function of heaters is to compensate the heat for incubating the reagent and formaintaining the temperature of the temperature-controlled chamber.

Fans are used in series in the temperature-controlled chamber. It makes the aircirculating in the chamber, and enhances the convective heat exchange. There arefour fans in the chamber. All have the alarm function.

The temperature transducer feeds back the air temperature at the position severalmillimeters from the bottom of the reaction cuvette.

The overheat protection switch is to switch off the power when the temperature

controller does work and the temperature-controlled chamber reaches 55, so as toavoid overheat or fire. When the temperature-controlled chamber becomes 35,

this switch will automatically be reset.

3.3.2 Reagent preheating

The preheating assembly consists of two aluminum plates, a Teflon tube having nineloop sections, heating components, transducer, temperature protection switch,thermal conductive colloid, a section of tube and the reagent probe.

The temperature of the thermal source of the preheater is controlled at 45. Theinitial temperature of the reagent is 4 ~ 10 when it is taken out of the refrigeration



System Descriptions

3-7

chamber. When the reagent passes the heater, its temperature increases to 35.

Then the reagent is added into the reaction cuvette and the preheating process isfinished.

Reagent preheating assembly

3.3.3 Reagent refrigeration

The refrigeration module consists of refrigeration cabin, PU heat-insulating sheath,reagent disk, reagent bottle, temperature transducer, refrigeration flakes, heatsinking component, fan and control circuit. The refrigeration module is shown in thefollowing figure.

The refrigeration assembly consists of fan, hot-end radiator, POM connector,cold-end heat-conductive aluminum block, and PELTIER refrigeration flake. Eachanalyzer has two such refrigeration assemblies, as shown in the figure below. Thecold-side of the refrigeration flake clings to the refrigeration compartment, and thehot-side clings to the radiator (The side having letters should cling to the refrigerationaluminum block).



System Descriptions

3-8

Each refrigeration flake corresponds to a heat-sinking block and a cooling fan. Itshould be installed with the cold side upward.

3.4 Photometric System

The photometric system consists of a measurement photometric system and areference photometric system. The measurement photometric system provides 9monochromatic lights to measure the absorbance of the reacting liquid in the rotatingreaction cuvettes. The reference photometric system compensates themeasurement photometric system to make the measurement more accurate.

共路

传光束小端

卤钨灯聚光镜传光束小透镜反应杯滤光片小透镜光电管

光电管

参考光路

测量光路

Tungsten-halogen

lamp Biconvex lens Main fiber

Fibers

Plano

convex lens Cuvette Filter Plano

convex lens Photodiode

Reference light

Photodiode

.

.

.

Measurement

lights

3.5 Fluid SystemThe fluid system is shown in the following figure.



System Descriptions

3-9

Reagent syringe

Sample syringe

Mixing bar

As shown in the figure above, the fluid system consists of interior washing andexterior washing.

The syringe assembly controls the aspiration volume by controlling the travel of thesample/reagent syringe. It is the core part of the fluid system.



System Descriptions

3-10

3.6 ISE Module (optional)

The ISE module that is used to measure the concentration of K+, Na

+, Cl

- and Li

+ in

serum, plasma and urine consists of ion-selective electrodes, peristaltic pumps andcalibrants.

Waste

ISEPump W Pump BPump A

Calibrant B Calibrant A



System Descriptions

3-11



Functions of Boards

4-1

4 Functions of Boards

The analyzer is integrated with the following boards:

Main control board

Power drive board

Sample level detection board

Reagent level detection board

A/D conversion board

Ten photoelectric conversion boards (340nm, 405nm, 450nm, 510nm, 546nm,578nm, 630nm, 670nm, 700nm and reference light)

Reagent refrigeration board

Power supply assembly

Manipulator connection board

Feeder connection board

Probes connection board

Main control board

Power drive board

Power supply assembly

PFC board

Heaters of reaction

disk

Reagent pre-heater

PC

Level detection boards

A/D conversion board Build-in sample bar

code scanner ISE module

Step motors

DC motors

Lamp

Reagent

refrigeration board

Peltiers

transducers

Transducers12V&5V board

24V board

10 photoelectric

conversion boards

Pumps, valves

ISE power supply board

4.1 Main Control Board

The main control board is the control center of the whole hardware system. Itconsists of the control circuits of 6 functional units (including main control unit,photoelectric unit, reagent unit, sample unit, loading/mixing unit, temperature controlunit). Each functional unit has an MCU. They communicate in the multi-unit modeand thus compose the whole control system.

The functions of the main control board are:

communicating with the PC through the RS232, receiving and analyzinginstructions from the PC and sending data to the PC.

controlling photoelectric conversion through the interface with the A/D conversionboard, reading and saving data from the A/D conversion board.

outputting control signals of each unit through the interface with the power driveboard.



Functions of Boards

4-2

receiving signals of fluid level detection and bump collision through the interfacewith the level detection boards.

detecting signals from temperature transducers and controlling temperature of thereaction disk and reagent preheating.

receiving signals from position transducers, deionized water transducer and wastetransducer and controlling the transducers.

controlling the built-in sample bar code scanner, reading the data and sending it tothe PC.

controlling the ISE module, reading the results and sending them to the PC.

4.2 Power Drive Board

The main functions of the power drive board are to receive the control signals fromthe main control board and control drive components. The block diagram of thepower drive board is shown in the figure below.

Control signals from

Main Control Board

to 13 step motors to 2 DC motors

to 2 solenoid valves to 3 pumps

to 2 heaters of

reaction diskto lamp power

to reagent

preheating

to 2 magnets

4.3 A/D Conversion Board

The 10 photoelectric conversion circuits convert the intensity signals of the lightstransmitting through the reaction cuvettes to electric signals, and then transmit themto the A/D conversion board through a 5-core shielded cable. Photoelectricconversion boards for different wavelengths have different gains and cannot bereplaced by each other.

The A/D conversion board filters and amplifies the 10 channels of electric signalsoutput from the photoelectric conversion boards, transmits them through themulti-way gating switch to the A/D converter and then sends them to the main controlboard for further processing.

4.4 Reagent Refrigeration Board

The circuits of the reagent refrigeration board include the refrigeration circuit and thefan circuit.

The refrigeration circuit is needed to work continuously, so it is powered separately.

The control objects of the reagent refrigeration board include:

Reagent refrigeration: 2 PELTIER components, 4 fans.



Functions of Boards

4-3

Heat sink system for the whole device: 3 lamp-cooling fans, 4 temperature controlfans of the reaction disk.

4.5 Level Detection Boards

The level detection boards that include sample level detection board and reagentlevel detection board are used to detect the fluid level of sample and reagentseparately. When the analyzer aspirates reagents/samples, the probes dip into theliquid to a specific depth, so as to avoid carryovers that have impacts on test results,and to avoid air aspiration when the reagent/sample is insufficient.

4.6 Feeder Connection Board

The feeder connection board transfers the signals between the feeder transducerand the main control board, and connects the power drive board and the loadingmotor (DC).

Connections:

Connector Connected with

J91 Front transducer

J92 Back transducer

J93 Intermediate transducer

J94 No-cuvette transducerJ95 Pressure transducer

J96 Motor control wire (connected with the power drive board)

J97 Motor control wire (connected the motors)

J98 Cuvette-loading button

J99 Connection wire of the main control board

4.7 Manipulator Connection Board

The manipulator connection board transfers the signals between the manipulatortransducer and the main control board, and connects the power drive board and theelectromagnet.

Connections:


J101 Horizontal loading position transducer

J102 Vertical loading position transducer

J103 Electromagnet-closing transducer of the lower hand



Functions of Boards

4-4


J108 Electromagnet-closing transducer of the upper hand

J104 Drive wire of the electromagnet of the upper hand

J105 Drive wire of the electromagnet of the lower hand

J106 Drive wire of the electromagnet (connected with the power driveboard)

J107 Connection wire of the main control board

J109 Safeguard transducer (reserved)

J110 Safeguard transducer (reserved)

4.8 Probes Connection Board

The probes connection board transfers the signals between the sample/reagent leveldetection board and the main control board, inputs the temperature signals, outputsthe reagent preheating signals, and transfers the signals between the power driveboard and the mixing motor (DC).


J200 Interface of the sample level detection board

J201 Interface of the reagent level detection board

J202 Interface of the mixing motor (DC)

J203 Sample detection signal interface of the main control board

J204 Reagent detection signal interface of the main control board

J205 Temperature control signal interface of the reaction disk

J206 Reagent preheating signal interface

J207 Interface of the power drive board

4.9 Power Supply Assembly

The power supply assembly consists of three boards: PFC board, 24V board,12V&5V board and ISE (optional) power supply board.

The functions of the PFC board include:

AC/DC conversion;

Supplying the +390V and VDD voltage to the 24V board and the 12V&5V board;

Supplying stable 12V voltage for the lamp;

Supplying control signals of the analyzing unit switch to control the C12V, 5V and24V outputs.

The 24V board converts the 390VDC current transmitted from the PFC board to theseparated 24VDC outputs through the forward converter.



Functions of Boards

4-5

The 12V&5V board converts the 390VDC voltage from the PFC board to B12V,C12V and 5V voltages through the forward converter.

The ISE power supply board converts the 12V of the reagent refrigeration board to24V that provides power supply for the ISE unit (optional).



Maintenance and Service

5-1

5 Maintenance and Service

WARNING:

Before disassembling or assembling the analyzing unit, ensure thePOWER is placed to OFF.

The probe tip is sharp and can cause puncture wounds. To preventinjury, exercise caution when working around the probe.

BIOHAZARD:

Wear gloves and lab coat and, if necessary, goggles.

Dispose of the waste in accordance with your local or nationalguidelines for biohazard waste disposal.

CAUTION:Please use Mindray-recommended consumables. Other consumablesmay decrease the system performance.

Refer to the BS-300 Chemistry Analyzer Operation Manual for details about

unclogging the sample probe

unclogging the reagent probe

replacing the sample probe

replacing the reagent probe

replacing the mixing bar

replacing the plunger assembly of syringe

replacing the lamp

5.1 Replacing Light Filter Assembly

The light filter and optical assembly are fixed in the supporting sleeve. The back endis compacted and enclosed with the photoelectric amplification board and the screengland. Generally, the supporting sleeve is replaced together with the filter and opticalassembly.




5-2

Figure 5-1 Light filter assembly

Supporting Sleeve

GasketFilter

Flat Spring

Lens

GasketLens Seat

Photoelectric

Conversion Board

Screw Shield Box

WARNING:

Before operating, ensure to place the POWER (main switch) to OFF.

1 Unscrew (counter clockwise) the two cap screws on the screen glandwhose wavelength is to be replaced.

2 Open the cover of the A/D conversion board, and unplug the plugcorresponding to certain wavelength.




5-3

3 Take out the photoelectric conversion board and the supporting sleeve.

4 Keep the photoelectric conversion board upward, and loosen the tworetaining screws on it.

5 Keep the photoelectric conversion board upward, and pull the photoelectricamplification board out of the supporting sleeve.

6 Unpack the new supporting sleeve containing the optical assembly. Be sureto keep the top side (where to assemble the photoelectric amplificationboard) of the supporting sleeve upward.

7 Install the original photoelectric conversion board onto the new supportingsleeve, and then fasten the two retaining screws.

8 Install the screen gland, and fasten the retaining screws.




5-4

9 Connect the photoelectric conversion board to the A/D conversion board,and assemble the cover.

WARNING:

When replacing the light filter assembly, do not touch the opticalassembly in the supporting sleeve and the photoelectric receiving tubeof the photoelectric conversion board by hand.

The light filters and the photoelectric conversion boards are inone-to-one relationship. Do not disarrange them.

5.2 Replacing Optical Fiber

WARNING:

Before operating, ensure to place the POWER (main switch) to OFF.

1 Unscrew the four screws on the supporting pillars of the cuvette feeder, andremove the cuvette feeder.




5-5

2 Unscrew the four screws on the reaction disk cover, and open the reactiondisk cover.

Attention should be paid to the power cable of the upper heater when thereaction disk cover is being opened.

Power cable ofupper heater

3 Take out two cuvette segments in a diagonal with the needle-nose pliers tomake two spaces for disassemble the colorimetric disk.

4 Unscrew three M3 cap screws, and then disassemble the colorimetric disk.




5-6

5 Use an M3 cap screwdriver to loosen the retaining screws of the opticalfibers on the colorimetric clamp and the reference light support.

6 Take out the optical fibers one by one, and fix the optical components in the

colorimetric clamp by fastening the screws slightly.

Screw of the reference light

7 Draw out all the nine optical fibers from the reaction compartment.

8 Loosen the M3 cap screw (used for retaining optical fibers) on the lamphousing, and then draw out the optical fibers.

9 Put nine of the ten branches of the new optical fiber into the reaction diskfrom its bottom one by one, loosen the retaining screw, insert the opticalfiber to the end, and then fasten the retaining screw.

10 Fix the reference light optical fiber.




5-7

11 Fix the optical fiber of the lamp housing.

12 Put the colorimetric disk back and fix it.

13 Put the reaction disk cover back and fix it.

14 Install the cuvette feeder and fix it.

CAUTION:

When replacing the optical fiber, ensure that its bending radius is noless than 20cm. Otherwise, the optical fiber will be damaged.

5.3 Adjusting Reaction Disk, Manipulator and Feeder

NOTE:Debug the lower arm first (The relation between the lower arm positionand the reaction cuvette position is very important.), and then the upperarm. When debugging the lower arm, move the cuvette compartmentaside.

1 Disassemble the cuvette feeder, and adjust the circular position of thereaction disk (through the initial position transducer of the reaction disk) tothe standard position (The finger of the lower arm points to the center of theslot between reaction cuvette segments).

2 Horizontally adjust the manipulator to the standard position (The finger ofthe lower arm can work on reaction cuvettes well, and it should be 0.2mmaway from the nearest point of the reaction cuvette).




5-8

3 Vertically adjust the manipulator the standard position (The finger of thelower arm can work on reaction cuvette well, and the finger support of thelower arm should be 0.15mm above the reaction cuvette.).

4 Run the manipulator to the position for taking new cuvettes, andhorizontally adjust the reaction cuvettes in the feeder. When catching acuvette, ensure a clearance of 0.4mm ~ 0.6mm between the finger of theupper arm and the cuvette, and center them.

5.4 Adjusting Probes and Disks

NOTE:

Adjust the positions of the three probes and reaction cuvettes, and thenothers.

Adjust the working position of the reagent probe (To minimize thecumulative error, ensure that the reagent probe return to the initial positionbefore each adjustment.) as follows:

A For the reagent discharging position, ensure the reagent probe is inthe center of the reaction cuvette and the probe tin is 2 ~ 3mm awayfrom the bottom of the reaction cuvette.

B Adjust the position of the initial-position transducer of the reagentdisk. Ensure the mouths of reagent cuvettes in the inner and outercircles fit the reagent disk cover well.

C Adjust the washing position of the reagent probe. Ensure the reagentprobe is in the center of the wash well and the probe pin is 5mmaway from the bottom of the wash well. If necessary, adjust theposition of the wash well.

1

D Adjust the reagent probe’s position on the outer circle of the reagentdisk. Ensure the reagent probe is in the center of the hole of the outer

circle.




5-9

E Adjust the reagent probe’s position on the inner circle of the reagentdisk. Ensure the reagent probe is in the center of the hole of the innercircle.

Adjust the working position of the sample probe (To minimize thecumulative error, ensure that the sample probe return to the initial positionbefore each adjustment.) as follows:

A For the sample discharging position, ensure the sample probe is inthe center of the reaction cuvette and the probe tin is 2 ~ 3mm awayfrom the bottom of the reaction cuvette.

B Adjust the position of the initial-position transducer of the sampledisk. Ensure the mouths of sample cuvettes in the inner and outercircles fit the reagent disk cover well.

C Adjust the washing position of the sample probe. Ensure the sampleprobe is in the center of the wash well and the probe pin is 5mmaway from the bottom of the wash well. If necessary, adjust theposition of the wash well.

D Adjust the sample probe’s position on the outer circle of the sampledisk. Ensure the sample probe is in the center of the hole of the outercircle.

2

E Adjust the sample probe’s position on the inner circle of the sampledisk. Ensure the sample probe is in the center of the hole of the innercircle.

Adjust the working position of the mixing bar.

A Switch off the analyzing unit first, and then rotate the mixing arm tosee if its rotation radius is proper. If the mixing bar cannot reach thecenter of the reaction cuvette, adjust the position of the mixing bar onthe mixing arm properly.

B Disassemble the mixing arm, and then switch on the analyzing unit torun the reaction disk and stop it at any cuvette. Hold the mixing armby hand, and fix it at the center of the reaction cuvette.

C Vertically adjust the mixing bar’s position in the reaction cuvette.Ensure the mixing bar tip is 1 ~ 2mm away from the bottom of thereaction cuvette.

3

D Adjust the washing position of the mixing bar. Ensure the mixing baris in the center of the wash well and about 5mm away from thebottom of the wash well. If necessary, adjust the position of the washwell.

4 In the engineering adjustment software, select the Debug instruction menu, and rotate the reaction disk for one lap. Dive probes and mix thereactant at every position or every several positions to see if the threeprobes interfere with any reaction cuvette (Command 61). If the mixing barknocks the side or the bottom of any cuvette, repeat Step 3.




5-10

5.5 Replacing Components of ISE Unit (optional)

5.5.1 Replacing Tubing

NOTE:

(1) Fluidic tubing can be divided into the following six types.

W1 with two adapters connects the ISE module and the pump W.

Pump module

ISE module

Reagent module to be

placed here

Pump tubing

Pump tubing adapter

Pump B Pump A Pump W

Pump head

Fluidic tubing adapter

W2 connects the reagent module and the pump W.

A1 (B1) with one adapter connects the sample entry port and the pump

A (pump B).

A2 (B2) with two adapters connects reagent module and the pump A(pump B).

They are different in length and W1, A2 and B2 are not directionalwhen they are installed.

Usually the adapter has not been mounted to the fluidic tubing, so youhave to do it by hand. The tubing should be mounted to a short metallictube on the adapter. For convenience, the connecting end of the tubingcan be dipped into hot water for several seconds.

(2) Pump tubing is used around a pump head. It has one adapter on

each end that makes connection with the fluidic tubing easy.




5-11

5.5.1.1 Replacing Fluidic Tubing W1 and W2

1 Place the POWER to OFF.

2 Open the ISE unit door.

3 If you want to replace the W1, unscrew the screw of the ISE module andtake off the cover.

4 Put out the fluidic tubing W2 and insert it into a container such as a cup thatis used to contain the waste solution flowing from the W2.

5 Start the analyzing unit and the system software.

6 Enter the ISE Maintenance screen of the system software.

7 Click the Maintenance button several times until there is no solutionflowing out from the W2.


9 If you want to replace W1, pull out its two adapters directly from the wastepump tubing adapter and the right angle adapter that is fixed to thecompression plate.

Right angle adapter

The side matches

the adapter of

fluid tubing W1

The side with a recessmatches the compression

plate of the ISE module.

Note that when pulling out the fluidic tubing adapter, in order not to releasethe right angle adapter, you can hold the right angle adapter with a finger.

After that, install a new W1. For W2 exchange, it just needs to replace theW2 with a new one.

10 If you want to replace the W2, replace it with a new one. Otherwise, insertthe W2 back to the reagent module.

11 Place the POWER to ON.

12 Enter the System Maintenance screen of the system software.

13 Select the Others tab and click the Download Settings button.

14 Enter the ISE Maintenance screen.

15 Click the A purge button to observe if there is solution leaking out. If thereis solution leaking out, repeat from the step 4 to install the tubing again.

16 Install the cover of the ISE module if it has been taken off.

17 Close the ISE unit door.




5-12

5.5.1.2 Replacing Fluidic Tubing A1, A2, B1 and B2


2 Take off the panel under the sample disk and you can see the shielding boxand the peristaltic pumps.


4 Take out the reagent module.

5 Put the fluidic tubing W2 in a container such as a cup that is used to containthe fluid flowing out from the W2.



8 If you want to replace A1 or A2, click the A purge button. If you want toreplace B1 or B2, click the B purge button.

Repeat this step for several times until the received data indicates that theCalibrant A or Calibrant B has air bubble in it.


10 Replace the tubing with a new one.

Note that before installing the A1 or B1 to the sample entry port, you candip the connecting end of the A1 or B1 into hot water for several minutes tomake the following procedures easily performed.

11 Start the analyzing unit.

12 If the system software is not running, start it. Otherwise, enter the System Maintenance screen of the system software, select the Others tab and

click the Download Settings button.


14 If you have replaced A1 or A2, click the A purge button. If you havereplaced B1 or B2, click the B purge button.

Repeat this step for several times until the received data indicates that theCalibrant A or Calibrant B has no air bubble in it.

5.5.1.3 Replacing Pump Tubing



3 Pinch one adapter of the pump tubing and take it out from the tubing shelf.Then take off the whole tubing.

Tubing shelf




5-13

4 Put one adapter of the new pump tubing onto the tubing shelf and make thetubing around the pump ahead, then put the other adapter onto the shelfwith a little strength.



7 Click the Pumps button to see if the calibration of pump is correct. If not,repeat the upper steps.

5.5.2 Replacing Pumps


2 Take off the pump tubing around the pump head.

3 Disconnect the cable and the pump motor.

Pump motor

Cable

4 Unscrew the four screws around the pump head.

5 Pull out the pump directly.

6 Put a new pump on the pump shelf, tighten the screws, connect the calbeand pump motor, and then put the pump tubing around the pump head.



9 Click the Pumps button to see if the calibration of pump is correct. If not,repeat the upper steps.

5.5.3 Replacing ISE Module

Some times there is something wrong with the components in the ISE module suchas sample entry port, bubble detector, PCB and so on. In these cases, the ISEmodule should be replaced according to the following procedure.


2 Take off the panel under the sample disk and you can see the shielding boxand the pumps easily.







5-14

6 Click the Maintenance button.


8 Unscrew the screw of the ISE module and take off the cover.

9 Take off all electrodes from above to below.

10 Disconnect the right angle adapter and the compression plate.

11 Disconnect the cable.

12 Unscrew the four screws that fix the ISE module to the shielding box. Thentake out the module from the shielding box.

13 Put a new ISE module to the shielding box. Then connect the cable andinstall the electrodes sequentially. At last mount the right angle adapter tothe compression plate.

14 Install the electrodes sequentially.

15 Enter the System Maintenance screen of the system software.

16 Select the Others tab and click the Download Settings button.


18 Click the A purge button to see if there is solution leaking out. If there is,take off the electrodes and install them again.

19 Check if the new ISE module can work normally.



Software Introduction

6-1

6 Software Introduction

The software of BS-300 analyzer is composed of the system software and the control

software.

6.1 System Software

The system software can, according to the requirements and inputs of users,generate a work schedule (instruction sequence), control the units of analyzing unit inthe sequence of instructions in the work schedule, receive photoelectric data,response messages or execution results from the analyzing unit, output them to thePC screen or the printer. With these outputs, users can obtain correct test results.

According to different functions, the entire PC software system can be divided into the

following parts:

System Initialization

This part includes the initialization of the PC operating system, the initialization of thecommunication between the PC and analyzing unit, and the controls of analyzing unitreset.

Control system

This part includes the formation of the work schedule, instruction data sending andreceiving.

GUI

This part includes the requests for tests (routine tests, emergency tests, calibrationtests and QC tests), the observation of test status (status of the reaction disk, sampledisk and reagent disk), test management, calibration management, QC management,result query, alarm management and the help system.

Shutdown Processing

This part includes the resets of sub-units.

6.1.1 System initialization

To initialize the PC operating system,

Check the PC operating system. The system software must run under Windows2000 or Windows XP. Otherwise, the system will prompt that the system softwarecannot run under other operating system, and then the system software systemexits automatically.

Check the current screen resolution for the operating system. The system software

must run under the resolution of 1024 × 768. Otherwise, the system prompts toreset the resolution before restarting the system software and exit the systemsoftware.

Disable the screen protection program. The system software must keep displayedwhile running. To prevent the screen protection program from disturbing users inthe operating and observing processes, disable it.

Lock the keyboard. When running, the system software will lock some keycombinations to prevent users from starting any other program and conducting any




6-2

other operation. In this case, users cannot switch over to any other program orprint the current screen.

Check whether there is any username and password of themaintenance/debugging personnel in the registry. The system software has thedebugging function. Only the authorized maintenance/debugging personnel canenter the debugging window and maintain or debug the system and the host. For

the confidentiality, the username and password are saved in the registry. If theyare unavailable in the registry, the system will re-write the default username andpassword into the registry.

To initialize the communication and the units auto-check,

Set serial ports and initialize them, including such parameters as the baud rate,data bit, start bit, stop bit, parity bit, transmitting/receiving buffer, control protocoland so on. In addition, start the serial port receiving thread.

Handshake for communication. Send a handshake instruction to the analyzing unit.If the analyzing unit responds to this instruction (namely, send back a handshake

instruction to the PC), it indicates the PC handshakes with the host successfully. Ifthe host fails to respond to handshake instruction, it will re-send the handshakeinstruction back in a specific period. If it fails for continuously three times, thesystem will prompt to exit the system software. If you ignore that and continue toenter the system software, all tests cannot be conducted under the system.

Check whether the printer is connected. If not, the system will prompt to connect it.

Send an instruction for querying the auto-check results of the units, check theauto-check result of each unit. (The units are auto-checked when the analyzingunit is started, and the auto-check results are stored in the main unit.) In case ofany fault data in the auto-check result of any unit, the system will prompt to switchoff the analyzing unit and check the faulty unit.

6.1.2 Shutdown processing

In the BS-300 Chemistry Analyzer Control System window, click the Exit button.The Confirm dialog box appears. If you click OK, the system will

Switch off the lamp;

Unload all reaction cuvettes;

Wash the fluid tubing;

Reset all units;

Unlock the keyboard;

Prompt to exit the operating system and shut down the PC.

6.2 Control Software

The analyzing unit can be functionally divided into the following units: photoelectricunit, reaction disk unit, reagent disk unit, sample disk unit, reagent probe syringe unit,sample probe syringe unit, mixing bar unit, loading and manipulator unit, temperaturecontrol unit, fluid tubing unit and reagent refrigeration unit. The functions of thoseunits are listed in the following table.




6-3

Unit Function

Main unit Receives macroinstructions from the PC, decomposes theminto a series of action instructions, and then delivers theaction instructions to destination sub-units (in specific order)at a certain interval.

Monitors the status of other units, and transmits datacollected by the photoelectric unit to the PC.

Photoelectricunit

Photoelectrically detects, amplifies and converts the solutionin reaction cuvettes, and stores A/D converted data at thetwin port FIFO for the main unit to read and transmit them tothe PC.

Reaction diskunit

Contains 80 cuvette No.

Runs the reaction cuvette with the specified No., followingthe instruction of the main unit, to the reagent dispensingposition, sample dispensing position, mixing position, andphotoelectric detection position at all wavelengths.

Reagent diskunit

Contains 50 bottle positions.

Carries reagents, and runs the reagent bottles with specifiedNo., following the instruction of the main unit, to the reagentaspirating position.

Sample diskunit

Contains 60 tube positions in the inner and outer circles.

Carries samples, and runs the sample tubes with specifiedNo., following the instruction of the main unit, to the sampleaspirating position.

Reagent probe

syringe unit

Receives instructions from the PC, and controls the reagent

probe in aspirating a specific volume of reagent from thereagent bottle and dispensing it to the specified reactioncuvette.

Sample probesyringe unit

Receives instructions from the PC, and controls the sampleprobe in aspirating a specific volume of sample from thesample tube and dispensing it to the specified cuvette.

Mixing bar unit Receives instructions from the PC, and controls the mixingbar in mixing the solution in the cuvette that has been run tothe mixing position.

Runs the mixing bar to the wash well, and wash it after eachmixing process to avoid carryover.

Loading andmanipulatorunit

Controls the cuvette feeder and manipulator.

The feeder assembly is responsible for detecting whetherthere are enough reaction cuvettes in the cuvettescompartment and pushing reaction cuvettes to the positionfor the manipulator to take cuvettes.

The manipulator is responsible for taking reaction cuvettesfrom the reaction disk, placing them into the used cuvettebucket, taking new reaction cuvettes from the cuvettecompartment, and putting them on the reaction disk.




6-4

Unit Function

Temperaturecontrol unit

Controls the reaction disk temperature, reagent probepreheating temperature and fluid tubing.

Note: The reaction disk temperature should be kept at 37 ,the heating cavity of the reagent probe should be preheated

to 45.

Fluid tubing unit Controls the fluid tubing in washing the reagent probe,sample probe and mixing bar.

Reagentrefrigerationunit

Refrigerates the reagent chamber and controls itstemperature between 4 ~ 10 .



Service Flow

7-1

7 Service Flow

WARNING:

Before disassembling or assembling the analyzing unit, ensure thePOWER is placed to OFF.

The probe tip is sharp and can cause puncture wounds. To preventinjury, exercise caution when working around the probe.

When you disassemble or replace any board, ensure to wear antistaticgloves.

BIOHAZARD:

Wear gloves and lab coat and, if necessary, goggles.

7.1 Fluid Level Detection Failure of Reagent Probe

Surface

detection

failure of

reagent

probe(Th

e reagent

probe

does not

detect

the

surface

on the

reaction

disk.)

Thereagent

probe

cannot

detect

the

surface

on the

reagent

disk

The

reagent

probe mis-

detected

the surface

Surface detection signal transmission error: The surface detection

board does not have the working voltage, or when the probe touches

the surface, the indicator of the surface detection board is normal, but

there is always a host alarm indicating surface detection failure.

Possible cause: The patch cord for the reagent surface detection is

disconnected, or the connector is not well connected.

Surface detection signal processing error: When the probe touches

the surface, the indicator of the surface detection board is normal, the

connection between the main control board and the surface detection

board is normal, and no surface detection signal reaches the maincontrol board, but there is always a host alarm indicating surface

detection failure.

Possible cause: Main control board.

Surface detection board failure: When there is no probe

failure, but the indicator is always on, or the indicator is

not on when the probe touches the surface, there must

be a surface detection board failure.

Troubleshooting: Replace the surface detection board.

Power failure: The working voltage of the surface

detection board is 12V. In case of no power supply, the

surface detection signal cannot be generated.

Possible cause: Disconnection; the connector is not well

connected; there is no 12V output from the main control

board.

Probe failure: The detection terminal has a changeable

capacitance. Loose exterior or interior of the probe, the

sealing-off of the probe connecting wire and the probe

breakage will result in unsteady voltage or obvious

voltage changes. In this case, the capacitance signalwill be unavailable or exceed the range of the surface

detection board.

Possible cause: Reagent probe assembly, connector.

The surface

detection

signal fails

to be

generatedby

capacitance

change.

When this failure occurs, the sample probe cannot detect

the surface at the reaction disk, but there is no alarm at

the reagent unit. For details, see Surface Detection

Failure: Sample Probe.



Service Flow

7-2

7.2 Fluid Level Detection Failure of Sample Probe

Surface

detectionfailure of

the

sample

probe

The sample

probe cannot

detect the

surface at the

reaction disk.

The sample

probe cannot

detect the

surface at the

reaction disk.

The sample

probe mis-detects the

surface (In

this case,

there will be

no alarm, but

test results

will become

abnormal. On

the reaction

curve, you

can see that

no sample is

added.)

No reagent in the reaction disk: The reagent probe mis-

detects the surface, aspirates air and dispenses into the

reaction cuvette. Therefore, the reagent unit CPU fails to

make any judgment. In this case, the sample probe cannot

detect the surface when dispensing the sample into this

reaction cuvette.

Possible cause: Surface detection failure of the reagent probe

insufficient reagent: The reagent probe aspirates less reagent

than the set volume. As a result, the sample probe cannot

detect the surface at the preset height.

Possible cause: The reagent is prepared in the bottle, the

reagent syringe is not fixed, leakage occurs in the tubing of

the reagent probe, or there are bubbles in the reagent.

Position correction parameter error of the sample probe: In

case of such an error, the sample probe cannot reach the

surface in the steps.

Troubleshooting: Re-correct the sample probe position.

Surface detection signal transmission failure: When the probe

touches the surface and stops, the indicator of the surfacedetection board is on for about 2 seconds. The output signal

of the surface detection board switches normally, but there is

always a host alarm indicating that no surface is detected.

Possible cause: The patch cord for detecting the sample

surface is disconnected, or the connector is not well

connected.

Surface detection signal failure: The indicator of the surface

detection board is not on or always on when the sample

probe touches the surface.

Surface mis-detection at the sample disk: In this case, there

will be no alarm, but the sample probe fails to aspirate the

sample, and the test result and reaction curve become

abnormal.

Possible cause: Sample probe, surface detection board,

surface detection patch cord.

Signal processing failure of the main control board: When the

probe touches the surface, the indicator works normally. But

there is always a host alarm indicating that no surface is

detected. Ensure that the sample surface detection signal is

transmitted to the relevant connector of the main control

board.

Possible cause: Main control board failure

Surface mis-detection at the reaction disk: In this case, there

will be no alarm, but there is residual sample on the sample

probe tip, and drops on the table when the probe leaves the

reaction disk.

Possible cause: The sub-unit software is later than V1.7, or

the sample probe is not in the center of the reaction cuvette.



Service Flow

7-3

7.3 Liquid Dropping From Probes

Troubleshooting for the liquid

dropping fromthe reagent

probe and

sample probe.

Tubing leakage due to the abraded syringe

piston

Troubleshooting: Replace the syringe or the

syringe piston

The reagent probe takes liquid from the

reaction disk.

The reagent probe did not detect the surface atthe reaction disk, and dispenses the reagent at

the corresponding height. If the reagent probe

cannot touch the surface at the position fordispensing reagent, the last reagent drop will

be taken out by the reagent probe.

Possible cause: The probe arm is installed at atoo high position; the position of the sample

probe has not been corrected; the syringe is

loose; there are bubbles in the reagent bottle.

Probe being bent

Troubleshooting: Replace the probe.

The sample probe takes liquid from the reaction

disk.The sample probe detects the surface and

stops immediately. Then it dispenses thesample at 1mm under the surface. If the

reagent probe mis-detects the surface, the

dispensed sample will fail to be added into thereagent, but taken at the probe tip and drops

when the probe is moving. As a result, some

test results become abnormal.

Possible cause: There are bubbles in thereaction cuvette; the sample probe is not in the

center of the reaction cuvette, and its position

has not been corrected.

Probe driving assemblies are not assembled

well, resulting in probe jittering and liquid

dropping in the vertical movements.Troubleshooting: Adjust or replace the probe

driving assemblies.

Drips taken from the reagent disk or sampledisk and dropping from probes

Such failures are usually followed by alarms

indicating that no surface is detected at thereagent/sample disk. The reagent/sample

probe does not aspirate reagent/sample, butthe reagent/sample probe will stretch out to thebottom of the reagent bottle/sample tube due to

surface detection failures. As a result, reagent/

sample liquid will be taken on the exterior ofthe probe and drop while the probe is moving.

Other failures

Drips drop from the sample probe

due to fluid tubing leakage. In this

case, there will be liquid dropping

onto the table widely, and there maybe liquid dropping even when the

sample probe is static.

Drips drop from the sample probe

due to surface detection failures. In

this case, most liquid drop on thearea between the reaction disk and

the wash well.

Smudges on the sample probe absorb some

liquid and drop it while the probe is moving.Troubleshooting: Cleaning the sample probe.



Service Flow

7-4

7.4 Failing to Detect Level of Water for Washing Exteriors

Surface detection signal failure:

See relevant resolutions for the

surface detection failure.

No water in the

wash well

Reagent or sample probe.

Solution: Replace a probe

Failing to

Detect the

Surface of

the Water

forWashing

Exteriors

No water in the wash water tank: Users

can see this failure in the alarm

information detergent empty . In case of

no alarm information, check whether

there is any problem in the detergent tank

cover assembly.

Pump damaged: In this case, there will

be no water in the three wash wells.

Troubleshooting: Replace the pump.

Tubing blocked: In this case, there will be

insufficient water for washing exteriors,

the three water flows are not the same, or

there will be no water in any of the three

wash wells.

Possible cause: The unidirectional valve

is blocked, or the detergent entrance is

blocked.

The tube is disconnected.

Possible cause: The tube is degraded, or

other causes.

Troubleshooting: Connect the tubes

again.



Service Flow

7-5

7.5 Abnormal Results

7.5.1 All Results Being Abnormal

All test

results are

abnormal

All results are

lower than

normal

results.

Some results

are higher,

and some are

lower, with

bad

repeatability.

No sample or insufficient sample is added.

In this case, it can be seen on the reaction curve that

the absorbance has not increased in the period for

adding the sample. The reaction curve is even, and

many results are 0.

Possible cause: Sample probe blocked, tubing

leakage, syringe not fixed, valve not shut tightly.

The reaction is not thorough.

If the sample is not mixed uniformly with the reagent,

the reaction will not be thorough, and the light cannot

detect the changes of the absorbance.

Possible cause: The mixing bar did not mix the

reacting liquid.

The volumes of dispensed sample are not the same.

In this case, it can be seen from the reaction curve

that the response is proportional to the volume of the

sample.

Possible cause: Tubing leakage, valve not shut

tightly, or cuvette segments not cleaned well.

7.5.2 Some Results Being Abnormal

Some test results are

abnormal.

Good repeatability

Troubleshooting: Re-calibrate it.

Bad repeatability

Observe the reaction curve, and find the possible

causes.

All test results are approaching to 0.

Observe the reaction curve first.

Possible cause: The lamp base of correspondingwavelength exceeds 65535; the photoelectric

detection unit fails to detect the change of

absorbance, reagent failure and optical fiber

disconnection.



Service Flow

7-6

7.5.3 Several Results Being Abnormal

Several test results are

abnormal

Several test results are too low.

In this case, observe the reaction curve first.

Possible cause: Drips dropping from the sample

probe

Several test results suddenly become too high.

In this case, observe the reaction curve first.

Possible cause: There are bubbles in the current

cuvette; the mixing bar knocks the cuvettes.



Service Flow

7-7

7.6 Insufficient Light Intensity of Lamp

Estimate whether the

lamp has worked for

2000h (lifetime),

replace Lamb Replace the lamp.

Check whether the AD value of the

channel whose lamp vase value is

smaller increases obviously.

Record the lamp background value and gain

Measure and record the lamp background value and gain

Replace lamb

Insufficient Light

Intensity of the Lamp

Lamp background values of all

channels decreases.

Lamp base values of several

channels decreases

Adjust the gain parameter to make the

lamp base AD values of all channel

lamps increase to about 62000.

Y E

S

YES

Replace the lamp with

the original lamp

Replace the photoelectric transducerassembly, and then check whether the

lamp base value increases obviously.

Replace the

photoelectric

transducer assembly.Change the positions with the

smaller fiber end, and check

whether the lamp base value

increases obviously.

Check whether the photometric

system (such as the lens)

corresponding to the fiber channelis covered with dirt or the like. (If

yes, clean it.) Then check whether

the lamp base value increases

obviously

Replace the optical

fiber

Clean thephotometric system

(such as the lens)

Remove the fault alarm

N O

YES

NO

YES

N O

YES

Note:

Do not adjust the photoelectric gain parameter in case you have no idea about thecause for the alarm indicating insufficient light intensity of the lamp.



Service Flow

7-8

7.7 Temperature Control Failure

Temperature control failure

Query and record the

fault log codes and

descriptions.

Monitor the temperature

curve.

Obtain the current

temperature control

parameter, and check

whether it is the same as the

factory parameter.

Enable and disable the

temperature control function

repeatedly for 2 ~ 3 times

Check whether the temperature

transducer is normal.

Check whether the heating

resistance is normal.

Replace the

temperature

transducer.

Replace the heating

assembly.

Restart the analyzer and thencheck whether it is normal.

y e s

NO

NO

Y E S

To monitor the temperature curve

A In the BS-300 Chemistry Analyzer Control System window, selectthe [System/Temperature] menu. A dialog box appears. EnterBS300DEBUG and MINDRAY respectively in the User Name andPassword text boxes. Then click OK to enter the TemperatureControl Curve window.

1

B Set a proper temperature range for observing the change of the

temperature curve, and then click the Start button.



Service Flow

7-9

C Minimize the Temperature Control Curve window for futureobservation at any time.

D After that, print or snap the temperature curve and then return it tothe headquarters together with the service work report.

To measure the impedance of the temperature transducer

A Switch off the BS-300 analyzer.

B Open the left side plate of the BS-300 analyzing unit to expose themain control board.

C Unplug J45 (reagent preheating temperature transducer), andmeasure the resistance between PIN1 and PIN3 with a multimeter.

The relation between the resistance and the temperature is: 25

/1100Ω, 38.5/1145Ω, 45/1170Ω, 50/1170Ω.

2

D Unplug J44 (reaction disk temperature transducer), and measure theresistance between PIN1 and PIN3 with a multimeter. The relation

between the resistance and the temperature is: 25/1100Ω, 38.5

/1145Ω, 45/1170Ω, 50/1170Ω.

To measure the resistance of the heater

A Measure the impedance of the reagent preheater in the followingprocedures:

Unplug J24 (power driving board)

Measure the impedance between PIN1 and PIN2. Reagent preheater(BA30-10-06626)/24.6 to 28.5Ω.

3

B Measure the impedances of the upper and lower heaters of thereaction disk in the following procedures:

Disconnect the connecting wire from J113 of the power patchingboard.

Measure the impedances of the upper and lower heater: uppertemperature-sensitive heater (BA30-10-06624)/437.8 to 483.8Ω,lower temperature-sensitive heater (BA30-10-06625)/131.4 to145.2Ω.

7.8 Bar Code Scanner (optional) Failure

WARNING:

Light sent by the bar code scanner may hurt your eyes. Do not stareinto the laser beam from the bar code scanner.

If the bar code scanner cannot emit laser, you can service it according to the followingsteps.

1 Place sample tubes with bar code labels that meet the requirementsmentioned in the operation manual.

2 Restart the analyzer.



Service Flow

7-10

3 Enter the Scanner Setup & Maintenance screen of the system software.

4 Enter the Maintenance tab.

5 Enter the sample position range in the Sample Position field and click theStep Scan button. Observe if the scanner can emit laser. If not, click theLaser on button. If the scanner still cannot emit laser, go to the next step.

If the bar code scanner emits laser normally but is failed in reading, you can service itaccording to the following steps.

1 Place the ANALYZING UNIT POWER to OFF.

2 Place sample tubes with bar code labels that meet the requirementsmentioned in the operation manual.

3 See if the window on the sample department is stained. If so, clean thewindow with absolute alcohol-dipped gauze and go to the next step. If not,go to the step 8.

4 Restart the analyzer.

5 Enter the Scanner Setup & Maintenance screen of the system software.


7 Enter the sample position range in the Sample Position field and click theStep Scan button. Observe the scanning procedures to see if the scannercan read the bar codes. If not, go to the next step.

8 Enter the Setup tab.

9 Select all symbologies but do not select any Check or Length.


11 Click the Scan button to see if the scanner can read the bar codes. If not,go to the next step.

12 Place the test fixture to the position 28 on the sample disk.

13 Click the Mechanism button at the System Maintenance screen to resetall mechanical parts. If the laser from the scanner cannot reach the barcode through the slit on the test fixture, adjust the screws on the brackets until the laser from the scanner can reach the bar code.



Service Flow

7-11

Bracket 1

Bracket 2

14 If the scanner cannot read the bar codes successfully, replace the bar codescanner. If the new scanner cannot read the bar code, you need to checkthe bar code labels the user used.

7.9 Feeder Failure

7.9.1 Transducer Distribution of the Feeder

Pressure Transducer

No-Cuvette

Transducer

Cuvette-Pushing

Limit Transducer Insufficient-Cuvette

Transducer

Cuvette-taking limit

transducer



Service Flow

7-12

7.9.2 Feeder Failure

Feeder failure

symptom

The trolley moves

backward to the

end, but does notmove forward.

Possible causes:

1. The cuvette-taking limit signal has not been

detected.

2. The belt is too tight, so the motor cannot run.

Troubleshooting:1. Check whether the voltage is 0 when the

cuvette-taking limit optical coupler is blocked.

2. Adjust the position of the motor to loosen the

belt.

The trolley moves

forward and stops

before compacting

the cuvette

segment

1. The moving resistance is too large, so the

trolley fails to compact the cuvette segment, but

the pressure transducer has been blocked.

2. The sliding block falls off, resulting in large

friction.

3. The motor cannot work normally.

4. The belt is too tight.

Troubleshooting:

1. Clean the guiding axis of the trolley.

2. Replace the sliding block assembly.

3. Replace the motor.

4. Loosen the belt.

The trolley keeps

moving backward

and forward

1. The no-cuvette transducer has not been

pressed completely, so the system deems that

the cuvette compartment is empty, and the

trolley moves back for cuvette segments.

Troubleshooting:

1. Adjust the position of the no-cuvette

transducer.

2. Replace the feeder assemblies.



Service Flow

7-13

7.9.3 Manipulator Failure

Manipulator

Failure

Manipulator vertical-

movement error:

Cannot reach/leave the

initial position.

The signal of the vertical optical coupler

has not been detected after the motor

runs for specified steps.

Possible causes:

1. The resistance of the lead screw is too

large (frequently).2. The optical couplers for the vertical

initial position cannot work (scarcely).

3. The vertical motor of the manipulator

cannot work (scarcely).

4. The optical coupler connector is not

connected well (infrequently).

5. The power driving board cannot work

(infrequently).

6. The main control board cannot work

(scarcely).

Troubleshooting:1. Lubricate the

lead screw after

cleaning it.

2. Replace

relevant

assemblies.

The lower hand of the

manipulator cannot

close/open.

Direct cause:

The finger signal has

not been detected afterthe electromagnet of

the manipulator is

switched on.

1. The operator has added too many

cuvette segments into the feeder (behind

the trolley). When the trolley moves

backward, the jaw is pressed back by the

cuvette segments; when it moves forward

again, the hand fails to open in time, so

the jaw may press one side of the trolley

only. As a result, the cuvette segment at

the front fails to corporate with the upper

hand of the manipulator well (frequently).2. The lower hand of the manipulator fails

to corporate with the reaction disk

position well (frequently).

3. The snap ring of the manipulator is not

flexible (infrequently).

4. The lower hand coupler of the

manipulator cannot work any more

(scarcely).

1. Press the

cuvette-taking

key after adding

cuvette

segments into

the feeder.

2. Adjust the

positions of thelower hand and

reaction disk.

3. Replace the

manipulator (Its

position must be

adjusted)

The upper hand of the

manipulator cannot

close/open.

Direct cause:

The finger signal has

not been detected afterthe electromagnet of

the manipulator is

switched on.


flexible (infrequently).

2. The coupler of the upper finger cannot

work any more (scarcely).

3. The upper hand of the manipulator is

not arranged well with the position of thefeeder/reaction disk (frequently).

4. The coupler connector is not

connected well (infrequently).

1. Adjust the

positions of the

upper hand and

the feeder/

reaction disk.

2. Replace themanipulator (Its

position must be

adjusted).

The upper/lower hand

of the manipulator

accidentally closes/

opens.

Direct cause:

After the manipulator is

switched on, the finger

coupler signal is

received, but the signal

changes suddenly

while the manipulator

is moving.


flexible (frequently).

2. The finger coupler of the manipulator

cannot work any more (scarcely).

Troubleshooting:

1. Replace the

manipulator (Its

position must be

adjusted).

7.10 Troubleshooting of ISE Unit (optional)

When there is something wrong with the ISE unit, an error code will be displayed atthe screen and saved in the log. The error code of the ISE unit has a format of 7 ASCII characters, the format of which is <1234567>.

Each digit represents a certain error.

Digit 1: Air in sample, Calibrant A, Calibrant B, Bubble Detector, mV range. Digit 2: Sample/Calibrant B mV range.



Service Flow

7-14

Digit 3: mV out of range for Calibrant A in Sample Cycle, or Calibrant B in UrineCycle.

Digit 4: Noise in mV of Calibrant B in Calibration Cycle, or Sample mV during aSample Cycle.

Digit 5: mV Noise in Calibrant A of the Calibration Cycle, or Sample Cycle, mVNoise in Calibrant B of the Urine Cycle.

Digit 6: mV Drift of calibrant A.

Digit 7: Slope of Sample value is out of range.

Each row represents where the error locates in.

You can find all the seven numbers in the table below digit by digit and then knowwhat is wrong with the ISE unit. 0 means no error.

For example, when you see <0001000>, you only need to find where the “1” locatesin the Digit 4 column and you will see it locates in the Na+ row. So you will know thecause of this error is the noise in mV of Calibrant B or sample related to the Na

+

electrode.

Digit 1 Digit 2 Digit 3 Digit 4 Digit 5 Digit 6 Digit 7Error

Air/Hardware

mV Out

Calib/Sample

mV Out

Cal A inCalib/SampleMode,Cal B inUrineMode

mVNoise

CalB/Sample

mVNoise


Cal ADrift

inSampleMode,Cal B inUrineMode

Out ofSlope/MachineRanges

Air inSample/Urine

S 0 0 0 0 0 0

Air in Calibrant A A 0 0 0 0 0 0

Air in Calibrant B B 0 0 0 0 0 0

Air in Cleaner C 0 0 0 0 0 0

Air in Segment M 0 0 0 0 0 0

Pump Calibration P 0 0 0 0 0 0

No Flow F 0 0 0 0 0 0

Bubble Detector D 0 0 0 0 0 0

Reagent ChipRead

R 0 0 0 0 0 0

Reagent ChipWrite

W 0 0 0 0 0 0

Store CalibrationValue Error

Q 0 0 0 0 0 0

Command Error T 0 0 0 0 0 0

No Error 0 0 0 0 0 0 0

Na+ 0 1 1 1 1 1 1



Service Flow

7-15

Digit 1 Digit 2 Digit 3 Digit 4 Digit 5 Digit 6 Digit 7Error

Air/Hardware

mV Out

Calib/Sample

mV Out


mVNoise

CalB/Sample

mVNoise


Cal ADrift

inSampleMode,Cal B inUrineMode

Out ofSlope/MachineRanges

K+ 0 2 2 2 2 2 2

Na+, K+ 0 3 3 3 3 3 3

Li+ 0 4 4 4 4 4 4

Li+, Na+ 0 5 5 5 5 5 5

Li+, K+ 0 6 6 6 6 6 6

Li+, K+, Na+ 0 7 7 7 7 7 7

Cl- 0 8 8 8 8 8 8

Cl-, Na+ 0 9 9 9 9 9 9

Cl-, K+ 0 A A A A A A

Cl-, Li+ 0 B B B B B B

Cl-, K+, Na+ 0 C C C C C C

Cl-, Li+, Na+ 0 D D D D D D

Cl-, Li+, K+ 0 E E E E E E

Cl-, Li+, K+, Na+ 0 F F F F F F

The following table displays the causes and the correction measures of some errors.Note that if the correction measure is the replacement of board, bubble detector orother components in the ISE unit, you have to replace the whole ISE unit. You canrefer to the chapter 5 for detailed information about some replacement procedures.

Symptom Problem Correction

1. No power

2. Communication failure Turn off the system and thenstart it again.

3. RS232 cable is disconnectedor damaged.

Move the panel of analyzing unitand reconnect or replace cablebetween the ISE unit and themain control board.

4. Module connector has beendamaged.

Replace board.

System does notrespond

5. Component failure on board. Replace board.



Service Flow

7-16


1. Misalignment of electrodes Remove and replace electrodes<45 mV/decade to reseatproperly.

2. Deterioration of calibratorsolutions Replace Calibrant B first andretest. If still low, replaceCalibrant A and retest.

3. Deterioration of sensingelectrode (low slope)

Replace problem electrodesand test.

4. Air bubble on referenceelectrode membrane

Remove electrode, tap todislodge bubble, replace, andrecalibrate.

5. Deterioration of referenceelectrode

Replace reference electrodeand retest.

Low Slope

Na+, K+, or Li+ <45mV/decade

Cl- <35 mV/decade

or

High Slope

Na+, K+, or Li+ >63mV/decade

Cl- >60 mV/decade

6. Module or fluid temperaturesexceed 37° C. (high slope) Monitor temperature. Changeinstrument location if ambient istoo great.

1. Deterioration of sensingelectrode

Replace problem electrodesand test.

Noise Error Flag

Single electrode

2. Electrical noise spike fromenvironmental source

a) Check for electrical noisecoincident with activation.

b) Check grounding of Module.

c) Component failure on Moduleboard. Replace board.

1. Deterioration of referenceelectrode


Noise Error Flag

Multiple electrodes

2. Electrical noise spike fromenvironmental source



c) Component failure on Moduleboard. Replace board.

1. May occur when newelectrode or new bottle ofCalibrant A is installed by

system.

Purge the Calibrant A andrecalibrate the module. If theelectrode is new it may initially

drift as it rehydrates over thecourse of 15 minutes.

Drift Error Flag

Single electrode

2. Deterioration of sensingelectrode

Replace the problem electrodeand test.

1. May occur when newelectrode or new bottle ofCalibrant A is installed onsystem.

Purge Calibrant A andrecalibrate the Module.

Drift Error Flag

Multiple electrodes

2. Deterioration of referenceelectrode.




Service Flow

7-17


3. Electrical spike fromenvironmental source.



c) Component failure on Moduleboard. Replace the board.

1. Insufficient sample pipettedinto Module sample entry port

a) Host instrument must deliver70 µL. Increase dispensedvolume.

b) Operator must placesufficient sample in sample cupto account for all testsprogrammed.

c) Fluid leak in electrodes.

Air in Sample

2. Sample not positioned a) Electrodes not seatedproperly. Reseat electrodes.

b) Pump tubing aging. Replace.

c) Pump tubing obstructed ortubing length is excessive.

1. Sample and Calibrant A aresegmented with air.

a) Electrodes are not properlyseated or compressed. Checkcompression plate, spring andseal. Reseat electrodes.

b) Ensure that all electrodes

and o-rings are in place.

2. Fibrin or salt is plugging theelectrode flow path.

a) Use Cleaning procedure forModule.

b) Disassemble Module andclean or replace electrode withplugged flow path.

3. Bubble detector ismalfunctioning.

Replace bubble detector.

Air in Sample andCalibrant A

4.Waste pump ismalfunctioning.

Replace waste pump.

Air in Calibrant B and Air in Calibrant A

1. Calibrant B and Calibrant Aare segmented with air.

a) Electrodes are not properlycompressed. Checkcompression plate, spring andseal.

b) Ensure that all electrodesand o-rings are in place.

c) Ensure tubing is connectedproperly.

d) Replace tubing.



Service Flow

7-18


2. Fibrin or salt is plugging theelectrode flow path.

a) Use Cleaning procedure forModule.

b) Disassemble Module and

clean or replace electrode withplugged flow path.

3. Waste pump malfunction. Replace waste pump, tubing.

4. Bubble Detector malfunction. Replace bubble detector.

1. Calibrant A pouch is empty. Replace Calibrant A with a newone, purge and recalibrate.

2. Tubing is disconnected. Reconnect or replace tubing.

3. Calibrant A pump is notworking properly.

a) Check electrical connections.

b) Replace pump tubing.

c) Replace motor.

Air in Calibrant A (no“Air” errors reported forSample or Calibrant B)

4. Tubing is plugged, split orcrimped.

Replace tubing



Mechanical Structure

8-1

8 Mechanical Structure

Reaction disk motorReagent disk motorSynchronous belts

Coder sensors




8-2

Reagent probe assemblySample probe assemblyMixing bar assembly

Reagent cooling assembly




8-3

Lamb assembly Big head of fiber

Fan of Lamb assembly




8-4

Optical assembly

Reaction disk TEMP.

control assembly

Small heads of fiber

Reference light assembly




8-5

Washing assemblies

PumpsSample syringeReagent syringe

Valves




8-6

A/D conversion board


Main control board Power drive board




8-7

Loading system

Manipulator

assembly




8-8

Top of mixing assembly

Top of sample assembly

Top of reagent assembly

Bar code scanner (optional)



Tools and Parts

9-1

9 Tools and Parts

9.1 Service Tools

S/N TOOL Quantity Specification Unit

1 Allen Key 1 set 1.5, 2.0, 2.5, 3.0, 3.5,4.0, 5.0

mm

2 Phillips screwdriver 1 set 3*75, 5*75, 6*100 mm

3 Flat-bladed screwdriver 1 set 3*75, 5*75, 6*100 mm

4 Long Allen Key 1 set 2.5, 3.0 mm

5 Tweezers 1 pair small /

6 Multimeter 1 common /

7 Movable Wrench 1 common /

8 Electric iron 1 30 VA

9 Stainless steel 1 0.5 (diameter), 15(length)

mm

10 Knife 1 common /

11 Pincers 1 common /

9.2 Parts

S/N P/N Name

1 BA30-20-06448 Cable for reagent probe assembly’s elevator motor

2 BA30-20-06449 Cable for reagent probe assembly’s rotary motor

3 BA30-20-06450 Cable for Sample syringe’s motor

4 BA34-20-63601 Cable for Reagent syringe’s motor

5 BA30-20-06452 Cable for sample probe assembly’s elevator motor

6 BA30-20-06453 Cable for sample probe assembly’s rotary motor

7 BA30-20-06454 Cable for mixing bar assembly’s rotary motor

8 BA30-20-06455 Cable for mixing bar assembly’s elevator motor

9 BA30-20-06458 Cable for reagent compartment motor

10 BA30-20-06459 Cable for sample compartment motor

11 BA34-20-63602 Cable for reaction compartment motor

12 BA30-20-06464 Cable for loading system motor

13 BA30-20-06468 Cable for Sample and reagent valves



Tools and Parts

9-2

S/N P/N Name

14 BA30-20-06476 26-pins sensor cable

15 BA30-20-06477 34-pins sensor cable

16 BA30-20-06480 Detection cable for reaction compartment’s fan

17 BA30-20-06481 Cable between CPU board and power drive board

18 BA30-20-06484 Cable for waste and distill water container

19 BA30-20-06485 Cable between CPU board and loading system

20 BA34-20-63651 Cable between CPU board and manipulator

21 BA30-20-06523 Temperature sensor cable of reagent cooling system

22 BA30-20-15291 Cable between CPU board and reagent detection board

23 BA30-20-15292 Cable between CPU board and sample detection board

24 BA34-20-63603 Cable between power drive board and mixing motor

25 BA34-20-63631 Reagent probe cable

26 BA30-20-15295 Sample probe cable

27 BA30-20-15296 Mixing bar cable

28 A21-000010--- Tubing

29 BA30-10-15051 Single-way valve

30 0040-10-32303 Tubing

31 0040-10-32304 Connector

32 0040-10-32305 Connector

33 M90-100014--- 1/4-28 UNF LOCK NUT-RED NYL LNS-3

34 M90-100015--- 1/4-28 UNF LOCK NUT-RED N NYL LNS-4

35 M90-100030--- 1/8 Y BARBED FITTING-WHITE NYL Y230-

36M90-100050---

BULKHEAD MOUNTED FEMALE LURE RED NYLONCCLR-3

37M90-100051---

BULKHEAD MOUNTED FEMALE LURE GREEN NYLONCCLR-4

38 M90-100071--- Tubing

39 BA30-30-15169 5-way connector

40 BA30-10-06666 Step motor

41 BA30-10-15041 synchronous belt (220 XL037)

42 BA30-10-15258 Door’s magnet (couple)

43 BA34-30-63559 Power drive board

44 BA30-30-06312 Reagent cooling control board

45 BA34-30-63613 Sample compartment assembly



Tools and Parts

9-3

S/N P/N Name

46 BA30-10-06365 Halogen Lamb (50W)

47 BA30-30-06691 REF pre-amplification board

48 BA30-30-15228 340nm amplification assembly

49 BA30-10-06367 Filter 340

50 BA30-30-06673 340nm pre-amplification board

51 BA30-30-15229 405nm pre-amplification assembly

52 BA30-10-06368 Filter 405

53 BA30-30-06675 405 pre-amplification board


55 BA30-10-06369 Filter 450



58 BA30-10-06370 Filter 510



61 BA30-10-06371 Filter 546



64 BA30-10-06372 Filter 578



67 BA30-10-06373 Filter 630



70 BA30-10-06374 Filter 670



73 BA30-10-06375 Filter 700


75 BA30-30-06565 Drive assembly of sample probe

76 BA34-30-63637 Syringe assemply

77 3100-10-49438 100ul Syringe

78 BA30-10-06651 500ul Syringe

79 BA30-10-15115 Step motor



Tools and Parts

9-4

S/N P/N Name

80 BA30-20-06594 3-way connector for syringe

81 BA30-21-15161 Sensor cable for reagent syringe

82 BA30-21-15162 Sensor cable for sample syringe

83 BA30-30-06634 Mixing bar assembly

84 BA30-21-15159 Cable for mixing bar assembly’s elevator motor

85 BA30-21-15160 Cable for mixing bar assembly’s rotary motor

86 BA30-30-06639 Fans of lamb assembly

87 BA30-30-06641 Washing pool assembly

88 0040-10-32302 Connector

89 0040-10-32303 Washer

90 BA30-21-15311 Valves assembly (KNF)

91 BA30-30-06702 Reaction compartment assembly

92 BA30-30-06715 A/D converter assembly

93 BA30-30-06752 Rocker of reagent probe

94 BA30-30-06304 Liquid detection board

95 BA30-30-06753 Reagent probe assembly

96 BA30-30-06761 Heat-block assembly

97 BA30-30-06768 Rocker of sample probe

98 BA30-30-14978 Sample probe assembly

99 BA30-30-06770 Rocker of mixing bar

100 BA34-20-63653 Loading system assembly

101 BA30-21-06488 Pressure sensor and cable for loading system

102 BA30-21-06490 Cable for cuvette-pushing limit transducer

103 BA30-21-06491 Cable for cuvette-taking limit transducer

104 BA30-21-06492 Cable for insufficient-cuvette transducer

105 BA34-20-63648 Board for loading system

106 BA30-21-06489 Cable for no-cuvette transducer

107 BA30-30-15034 Code sensor of reaction compartment

108 BA30-30-15095 Reagent compartment assembly

109 BA30-30-06780 Reagent disk handle assembly

110 BA30-30-15113 Driving assembly of reagent probe

111 BA30-21-15155 Sensor cable for reagent probe’s up-down sensor

112 BA30-21-15156 Sensor cable for reagent probe’s rotary sensor

113 BA30-30-15166 Coder sensor for reagent disk



Tools and Parts

9-5

S/N P/N Name

114 BA30-30-15167 Coder sensor for sample probe’s sensor

115 BA30-30-15284 Probes connection board

116 BA34-30-63557 Main control board

117 BA33-30-35098 Power supply module

118 BA33-30-35078 PFC Board

119 BA33-30-35082 24V Board

120 BA33-30-35080 12V&5V Board

121 TSB1-20-20399 Fan

122 BA34-20-63655 Manipulator assembly

123 BA33-30-35223 Power socket and switch assembly

124 BA34-20-63622 Reagent temperature control assemply (220V)

125 BA34-20-63614 Cover of sample compartment

126 BA30-30-15029 Cover of reagent compartment

127 BA34-20-63638 Reagent temperature control assemply (110V)



Maintenance And Test Software

10-1

10 Maintenance And Test Software

10.1 General

The maintenance and test software of the BS-300 Chemistry Analyzer is thesoftware that helps maintenance personnel debug and maintain the BS-300Chemistry Analyzer. Maintenance personnel can, through this software, checkwhether the probes, mixing bar, disks, fluidic system and photometric system ofthe BS-300 Chemistry Analyzer work normally, modify parameters, and executeindividual commands or combined commands.

Run the NewDebug.exe file to enter the Maintenance and Test window. SeeFigure 10-1.

Figure 10-1 Maintenance and Test window

Display Area

As shown in Figure 10-1, there are 4 tabs in the Maintenance and Test window:

Command

PARA and Speed

Temperature

Photoelectric




10-2

Note:

The buttons displayed in gray in software interface are notfunctional.

Following is a brief introduction to the software interface.

Display area

At the bottom of the window is the display area for displaying the frameinformation and fault/error information. The upper box is for frame information, andthe lower one is for fault/error information.

There are five types of frame information, including Result, Echo, Data,Command and Warning.

Select the check box in front of frame information to display relevant frameinformation. Click the check box again to de-select, and the frame information willnot be displayed.

Click the Clear button to clear the information displayed in relevant box. The twoClear buttons are respectively for two boxes.

Setup button

Click the Setup button at up right corner to show a dialog as shown in Figure 10-2,which is used to change communication port and set baud rate and check sumtype of the port. Check sum type includes odd, even and none.

Figure 10-2 Serial Port Setup

Communication Port

Baud Rate Check Sum Type

About… button

Clicking the About… button at up right corner of the interface, you can view theversion information of this software.

In the following sections, the 4 tabs mentioned above will be detailed respectively.




10-3

Note:

The units of parameters involved in this software are respectively:

Time: 50ms;

Sample (reagent) aspirating/dispensing volume: steps of motors;

Temperature:.

Temperature control coefficient: 0.01.

10.2 Command

In the Command tab, you can send commands to the analyzing unit and checkwhether probes, mixing bar, disks, fluidic system, photometric system, ISE unitand built-in bar code scanner work normally.

Figure 10-3 Command tab

Single Command Area Macroinstruction Area

As shown in Figure 10-3, the Command tab mainly consists of two parts: SingleCommand Area and Macroinstruction Area.

10.2.1 Single Command Area

In Single Command Area, there are 8 sub-tabs:

Main Unit

Reagent Unit Sample Unit




10-4

Load Unit

Temp Unit

Photoelectric Unit

ISE Module

Sample Bar Code Module

Check the analyzing unit for their counterparts.

There are several buttons in each tab. Click a button and you can send acommand to the counterpart on the analyzing unit.

Note:

After starting this software, you must click the Downloadparameters to Analyzing Unit button in the Main Unit sub-tab.Otherwise, the software will not operate the analyzing unit

successfully. After clicking the Enable modifying parameters button in the MainUnit sub-tab, you can modify the parameters in the PARA andSpeed tab.

Following are instructions to the action implemented by each command.

Main Unit

Command What it does

Download parameters to

Analyzing Unit

Configures running parameters for the

analyzing unit.

Reset all mechanical parts Moves all units of the analyzing unit to theirinitial position, and cleans reagent probe,sample probe and mixing bar.

Enable modifyingparameters

Switches on the switch for modifying parameterthrough this operation before modifyingparameter at PARA and Speed tab.

Disable modifyingparameters

Switches off the switch for modifying parameterthrough this operation after modifyingparameter at PARA and Speed tab.

Handshake with AnalyzingUnit

Checks that the communication cable worksnormally.

Send a direct instruction Edits and sends user-defined commands.

Reagent Unit


Reagent probe wash Cleans the reagent probe.

Pump, valve (interior) on Turns on the pump and the valves that controlflow of washing the interior of reagent probe.




10-5


Pump, valve (interior) off Turns off the pump and the valves that controlflow of washing the interior of reagent probe.

Pump, valve (exterior) on Turns on the pump and the valve that controlflow of washing the exterior of reagent probe.

Pump, valve (exterior) off Turns off the pump and the valve that controlflow of washing the exterior of reagent probe.

Probe above reaction disk Moves the reagent probe above the reactiondisk.

Probe above outer circle Moves the reagent probe above the outer circleof reagent disk.

Probe above inner circle Moves the reagent probe above the inner circleof reagent disk.

Probe tip above bottle top

(outer circle)

Moves the reagent probe until the probe tip a

few millimeters above the top of the reagentbottle in the outer circle of reagent disk.

Probe tip above bottle top(inner circle)

Moves the reagent probe until the probe tip afew millimeters above the top of the reagentbottle in the inner circle of reagent disk.

Probe down to outer circle Moves the reagent probe down to the reagentbottle in the outer circle of reagent disk.

Probe down to inner circle Moves the reagent probe down to the reagentbottle in the inner circle reagent disk.

Probe down to reaction disk Moves the reagent probe down to the cuvettein the reaction disk.

Probe tip above cuvette top Moves the reagent probe until the probe tip afew millimeters above the top of cuvette in thereaction disk.

Probe down to wash well Moves the reagent probe to its washingposition.

Probe above wash well Moves the reagent probe to the top of washwell.

Reagent disk rotate Rotates the reagent disk for specified circlesand stop it at specified bottle position. Inputrotating circles in the first box and position No.

in the second box.

Reagent disk rotate toposition

Rotates the reagent disk to specified bottleposition. Input position No. in the box.

Reagent syringe init Moves the reagent syringe to its initial position.

Reagent syringe full Moves the reagent syringe to its largest travel.

Reagent syringe empty Empties the reagent syringe.

Reagent unit reset Cleans the reagent probe and rotates thereagent disk to position No. 1.




10-6

Sample Unit


Sample probe wash Cleans the sample probe.

Pump, valve (interior) on Turns on the pump and the valves that controlflow of washing the interior of sample probe.

Pump, valve (interior) off Turns off the pump and the valves that controlflow of washing the interior of sample probe.

Pump, valve (exterior) on Turns on the pump and the valve that controlflow of washing the exterior of sample probe.

Pump, valve (exterior) off Turns off the pump and the valve that controlflow of washing the exterior of sample probe.

Probe above wash well Moves the sample probe to the top of washwell.

Probe above reaction disk Moves the sample probe to the top of reaction

disk.

Probe above outer circle Moves the sample probe above the outer circleof sample disk.

Probe above inner circle Moves the sample probe above the inner circleof sample disk.

Probe tip above tube top(outer circle)

Moves the sample probe until the probe tip afew millimeters above the top of the tube in theouter circle of sample disk.

Probe tip above tube top(inner circle)

Moves the sample probe until the probe tip afew millimeters above the top of the tube in the

inner circle of sample disk.Probe tip above cuvette top Moves the sample probe until the probe tip a

few millimeters above the top of cuvette in thereaction disk.

Probe down to reaction disk Moves the sample probe down to the reactioncuvette in reaction disk.

Probe down to wash well Moves the sample probe to its washingposition.

Probe down to outer circle Moves the sample probe down to the tube inthe outer circle of sample disk.

Probe down to inner circle Moves the sample probe down to the tube inthe inner circle of sample disk.

Sample disk rotate Rotates the sample disk for specified circlesand stop it at specified tube position. Inputrotating circles in the first box and position No.in the second box.

Sample disk rotate toposition

Rotates the sample disk to specified tubeposition. Input position No. in the box.

Sample syringe init Moves the sample syringe to its initial position.

Sample syringe empty Empties the sample syringe.

Sample syringe full Moves the sample syringe to its largest travel.




10-7


Sample unit reset Cleans the sample probe and rotate the sampledisk to position No. 1.

Load Unit


Mixer to the top of wash well Moves the mixing bar to the top of wash well.

Mixer move into wash well Moves the mixing bar to its washing position.

Mixer move to the top ofreaction disk

Moves the mixing bar to the top of reactiondisk.

Mixer move into reactiondisk

Moves the mixing bar to mixing position inreaction disk.

Mixer stir for a while Mixing bar stirs for a specified period.

Mixing bar rotates for a whileabove wash well

Moves the mixing bar above its wash well andthen rotates it for a specified period.

Take new cuvettes Moves the compartment trolley backwards toget new cuvettes.

Load New cup Rotates the reaction disk to specified position,then get new cuvettes with the manipulator andput them in reaction disk.

Unload cup Rotates the reaction disk to specified position,then get the cuvettes in reaction disk with themanipulator and put them in used-cuvette

bucket.

Motion of manipulator fingers Tightens or loosen manipulator’s fingers(grabbing mechanism).

After entering a value of 0-3 to the box, clickthe button and the fingers will motions. 0means both fingers tighten; 1 means the upperloosen and the lower tightens; 2 means theupper tightens and the lower loosens; and 3means both loosen.

Manipulator to vertical init.pos.

Moves the manipulator to vertical initialposition.

Manipulator to horiz. init.pos.

Moves the manipulator to horizontal initialposition.

To H-pos. for taking cuvettes Moves the manipulator to horizontal position forgetting cuvettes.

To V-pos. for taking cuvettes Moves the manipulator to vertical position forgetting cuvettes.

To H-position for placingcuvettes

Moves the manipulator to horizontal position forplacing cuvettes.

To V-position for placing

cuvettes

Moves the manipulator to vertical position for

placing cuvettes.




10-8


Move steps vertically Moves the manipulator for specified steps invertical way.

Move steps horizontally Moves the manipulator for specified steps inhorizontal way.

Loader unit reset Moves the mixing bar to its initial position,loosen manipulator’ grabbing mechanism andmove the manipulator to its initial position.

Temp Unit


Temperature control on Turns on the specified temperaturecontroller(s).

There’re two temperature controllers as

Reaction Disk and Reagent PreHeat.

Selects the check box(es) in front of acontroller(s), click the button, you can turn onthe selected controller(s).

Temperature control off Turns off the specified temperaturecontroller(s).

There’re two temperature controllers asReaction Disk and Reagent PreHeat.

Selects the check box(es) in front of acontroller(s), click the button, you can turn off

the selected controller(s).

Photoelectric Unit


Reaction disk rotate Rotates the reaction disk for specified circlesand stop it at specified cuvette position. Inputrotating circles in the first box and position No.in the second box.

Reaction disk rotate somepositions

Rotates the reaction disk for specified cuvettepositions, and input number of cuvettepositions in the box.

Rotate and measure Rotates the reaction disk, collects reaction dataduring rotating, and then stops it at specifiedcuvette position.

Turn on light Turns on the light.

Turn off light Turns off the light.

Test dark current Turns off the light and measures the darkcurrent at this time. The dark current data willbe displayed in the box below.




10-9


Test base current Unloads the first segment of cuvettes inreaction disk with manipulator, then rotates thereaction disk and during rotating measures thelight where places the first segment of cuvettesbefore. The data regarding base current will bedisplayed in the box below.

ISE Module


Handshake The ISE unit shakes hands with the main unit.

Maintain Executes the maintaining cycle to run the waste pump untilthe electrode flow path is cleared of fluid.

Detect Bubble Checks to see if the bubble detector is functioningproperly.

Cleaning Period Removes protein build-up from the ISE Module electrodes.

Calibrate Calibrates the electrodes of the ISE Module.

Correct Pumps Calibrates the peristaltic pumps of the ISE Module.

Measure Serum Measures serum or plasma samples on the ISE Module.

A Purge Purges Calibrant A solution through the tubing from thepouch of Calibrant A to the ISE Module.

B Purge Purges Calibrant B solution through the tubing from thepouch of Calibrant B to the ISE Module.

<DSPA> Pumps 200µl of Calibrant A to make it convenient for youto aspirate it from the sample entry port.

<SWBC> Displays the result of bubble detector checking for the lasttime.

<SWPC> Displays the result of pump correction for the last time.

<CKSM> Displays the software version of the ISE unit.

<DVON> After clicking this button, the results in mv will also bedisplayed when calibration, measurement or a sip cycle isfinished.

<MVON> After clicking this button, the results in mv will also bedisplayed when calibration or measurement is finished.

<DVFF> Disables the effect of clicking <DVON> or <MVON>.

Sample Bar Code Module


Set Select a symbology from the pull down list and click thisbutton. If Valid is selected, the symbology will be set as theone that the scanner can recognize. Otherwise, it will bethe one that can not be recognized.




10-10


Send Enter an instruction into the edit box and click this button tosend it to the analyzing unit.

Laser On Turns on the laser of the bar code scanner.

Laser Off Turns off the laser of the bar code scanner.

Handshake The bar code scanner shakes hands with the main unit.

Scan After entering the start position No. and the end one intoStart and End edit boxes respectively, click this button toscan bar codes on the selected positions.

10.2.2 Macroinstruction Area

Figure 10-4 Macroinstruction Area

Explanation for the

macroinstruction Macroinstruction

CommandsButton

Parameters of the

current command

A macroinstruction is a set of commands.

When selecting a macroinstruction from the pulldown list in macroinstruction area,you can see there’re various commands and comments regarding themacroinstruction. In addition, parameters of the command currently selected willalso be displayed on the interface.

In the macroinstruction area, after selecting a macroinstruction from the pulldownlist, you can

Click the Run button to run the selected macroinstruction;




10-11

Click the Pause button to suspend the macroinstruction which is running;

Click the Stop button to stop the macroinstruction which is running.

Note:

After all commands in each macroinstruction are executed, thesystem counts it as one time.

When the check box on the right of the macroinstruction pulldownlist is selected, the selected macroinstruction will be executed foronce; otherwise, the macroinstruction will be executed cyclically.

For each macroinstruction, the commands of it are executed one by one in thesequence in which they are listed. When the set delay expires after a command isexecuted, the next command is executed immediately. In case of an error in theexecution process, the macroinstruction will be stopped.

Following are instructions to the action implemented by each macroinstruction.

Macroinstruction Command What it does

B purge Purges Calibrant B solutionthrough the tubing from thepouch of Calibrant B to theISE Module.

A,B purge

A purge Purges Calibrant A solutionthrough the tubing from thepouch of Calibrant A to the

ISE Module.Reagent probe abovewash well

Moves the reagent probeabove its wash well.

Valve on, Pump on Turns on the pump and valvethat control the flow ofwashing the exterior ofreagent probe.

Check exterior fluidpath of reagentprobe

Pump off, Valve off Turns off the pump and valvethat control the flow ofwashing the exterior ofreagent probe.

Sample probe abovewash well

Moves the sample probeabove its wash well.

Valve on, Pump on Turns on the pump and valvethat control the flow ofwashing the exterior ofsample probe.

Check exterior fluidpath of sampleprobe

Pump off, Valve off Turns off the pump and valvethat control the flow ofwashing the exterior ofsample probe.




10-12


Reagent probe abovewash well

Moves the reagent probeabove its wash well.

Valve on, Pump on Turns on the pump andvalves that control the flow of

washing the interior ofreagent probe.

Check interior fluidpath of reagentprobe

Pump off, Valve off Turns off the pump andvalves that control the flow ofwashing the interior ofreagent probe.

Sample probe abovewash well

Moves the sample probeabove its wash well.

Valve on, Pump on Turns on the pump andvalves that control the flow ofwashing the interior of

sample probe.

Check interior fluidpath of sampleprobe

Pump off, Valve off Turns off the pump andvalves that control the flow ofwashing the interior ofsample probe.

Test Loader Reload cup looply The manipulator gets newcuvettes from thecompartment looply, and thenput them in the reaction disk.

Reaction disk to nextcup

The reaction disk rotates andstops. No. of position for

reaction disk to stop at willincrease.

Mix instruction The mixing bar moves intoreaction cuvette and mixesfor a specified period.

Test mixing bar inreaction disk

Mixer to vertical init The mixing bar moves tovertical initial position.


The reaction disk rotates andstops. No. of position forreaction disk to stop at willincrease.

Reagent probe toreaction disk

The reagent probe movesvertically into bottom ofreaction cuvette.

Test reagent probein reaction disk

Reagent probe tovertical init

The reagent probe moves tovertical initial position.

Test sample probe inreaction disk


The reaction disk rotates andstops. No. of position forreaction disk to stop at willincrease.




10-13


Sample probe toreaction disk

The sample probe movesvertically into bottom ofreaction cuvette.

Sample probe to

vertical init

The sample probe moves to

vertical initial position.

Reagent disk inposition 50 in nextperiod

Reagent disk rotates toposition 50 and in the nextperiod the reagent probe willaspirate urine diluent from theposition.

Dispensing 270µl ofurine diluent

Reagent probe aspirates207µl of urine diluent anddispenses it to a cuvette.

Dispensing 30µl ofurine sample

Sample probe aspirates 30µlof urine sample and

dispenses it to a cuvette. Themixing bar stirs it.

Washing mixing bar Washes the mixing bar.

First period Sample probe aspirates 70µlof diluted urine to the ISEunit.

Second period Sample probe aspirates 70µlof diluted urine to the ISEunit.

Third period Washes the sample probe.

Idle period Measuring.


Urine test (autodiluted)


First period Sample probe aspirates 70µlof diluted urine to the ISEunit.

Second period Sample probe aspirates 70µlof diluted urine to the ISEunit.

Third period Washes the sample probe.



Urine test (manuallydiluted)


10.3 PARA and Speed

In the PARA and Speed tab, you can query, configure or load parameters as well

as debug the units.




10-14

Figure 10-5 PARA and Speed tab

Parameters Buttons

As shown in Figure 10-5, the PARA and Speed tab mainly consists of two parts asunit parameter and several operating buttons.

Unit Name includes Main unit, Sample unit, Reagent unit, Photoelectric unit,Temp unit and Load unit.

At this screen, you can not only inquire and configure each unit parameters, butalso save and read relevant data. Now let’s introduce them respectively.

Inquire unit parameters

1 Select a unit in Unit Name.

2 Click Query button.

For example.




10-15

Configure a parameter for a unit


2 Click the Query button.

3 Edit the parameter if necessary. Otherwise, go to the next step directly.

When editing, you can directly select and modify the data to be edited.

4 Select a parameter to be configured. For example.

5 Click the Config button to configure the parameter for relevant unit of theanalyzing unit.

Configure all parameters for relevant units

1 Select units in Unit Name.

2 Click the Config All button to configure all parameters of the selected unitfor relevant units of the analyzing unit.

Save data


2 Click the Save button, then pop up a dialog.

3 Input a filename and click the Save button, you can save parameters of thecurrently selected unit to a .txt file.

Read Data

1 Click Load button, then pop up a dialog.

2 Select the relevant .txt file and click Open button, you can configureparameters in the file for relevant unit of the analyzing unit.

The following contents explain the adjustable parameters.




10-16

Reagent unit

Parameter Unit Default What the parameter means

H-pos. for washingreagent probe

Wholestep

42 Position correction of thereagent probe in the horizontaldirection when it is above its

wash well.H-pos. of reaction disk Whole

step-42 Position correction of the

reagent probe in the horizontaldirection when it is above thereaction disk.

H-pos. of reagent diskinner circle

Wholestep

215 Position correction of thereagent probe in the horizontaldirection when it is above theinner circle of reagent disk.

H-pos. of reagent diskouter circle

Wholestep

165 Position correction of thereagent probe in the horizontal

direction when it is above theouter circle of reagent disk.

Position correction ofreagent disk inner circle

Microstep

10 The position correction of thereagent disk when it stops atthe specified position accordingto the position No. of its innercircle.

Position correction ofreagent disk outer circle

Microstep

10 The position correction of thereagent disk when it stops atthe specified position accordingto the position No. of its outercircle.

Sample unit


Bar code positioncorrection of sampledisk inner circle

Microstep

18 Position correction of the innercircle of sample disk relative tothe outer circle when the barcode scanner scans specifiedpositions.

H-pos. for washing

sample probe

Whole

step

102 Position correction of the

sample probe in the horizontaldirection when it is above itswash well.

H-pos. of sample diskinner circle

Wholestep

202 Position correction of thesample probe in the horizontaldirection when it is above theinner circle of sample disk.

H-pos. of sample diskouter circle

Wholestep

165 Position correction of thesample probe in the horizontaldirection when it is above theouter circle of sample disk.




10-17


H-pos. of sample probein reaction disk

Wholestep

-106 Position correction of thesample probe in the horizontaldirection when it is above thereaction disk.

H-pos. of sample probein ISE

Wholestep

490 Position correction of thesample probe in the horizontaldirection when it is above thesample entry port of ISE unit.

Position correction ofsample disk inner circle

Microstep

10 The position correction of thesample disk when it stops atthe specified position accordingto the position No. of its innercircle.

Position correction ofsample disk outer circle

Microstep

10 The position correction of thesample disk when it stops atthe specified position accordingto the position No. of its outercircle.

V-pos. of sample probein ISE

Wholestep

163 Position correction of thesample probe in the verticaldirection when it is above thesample entry port of ISE unit.

Load unit


Manipulator h-positionfor placing cuvettes

Wholestep

70 Position correction of themanipulator in horizontaldirection when it places acuvette segment into thereaction disk.

Manipulator h-positionfor taking cuvettes

Wholestep

259 Position correction of themanipulator in horizontaldirection when it takes acuvette segment from thecuvette compartment.

Manipulator v-positionfor placing cuvettes

Wholestep

70 Position correction of themanipulator in vertical directionwhen it places a cuvettesegment into the reaction disk.

Manipulator v-positionfor taking cuvettes

Wholestep

768 Position correction of themanipulator in vertical directionwhen it takes a cuvettesegment from the cuvettecompartment.

Mixing bar h-positionfor washing

Wholestep

68 Position correction of themixing bar in the horizontaldirection when it is above itswash well.




10-18


Mixing bar h-positionfor mixing

Wholestep

70 Position correction of themixing bar in horizontaldirection when it is above thereaction disk.

Photoelectric unit


Photoelectric gainparameter 405

/ 127 Signal gain coefficient of A/Dconversion for wavelength of405nm.










/ 127 Signal gain coefficient of A/Dconversion for wavelength of

630nm.Photoelectric gainparameter 670




Photoelectric gainparameter referencelight

/ 127 Signal gain coefficient of A/Dconversion for wavelength ofreference light.



Start position ofphotoelectric collection

Microstep

25 Correction of the start positionwhen photoelectric collectionstarts.




10-19

10.4 Temperature

Figure 10-1 Temperature tab

ReagentTemperature Curve

Control Panel

Reaction Tray

Temperature CurveControl Panel

The Temperature tab consists of two parts:

Curve of reagent preheated temperature (upper part);

Curve of reaction disk (lower part).

The two temperature curves are controlled respectively through two control panelson the right.

Meanings of the parameters on the control panels are as follows.

Parameter What it does

Value Displays current temperature value on the temperaturecurve.

YRange Temperature range on Y-coordinate of the temperaturecurve. Input upper limit and lower limit of temperaturerange in the two boxes.

XRange X-coordinate point of the temperature curve.

Temp Intended temperature expected to achieve.

Period Interval for sampling.

After setting Xrange, Yrange and Temp on the control panel, click Confirm buttonto implement them on relevant temperature curve.

There are 4 more buttons in each of the two control panels:

Start: After setting Period, clicking it, you can start collecting the temperaturedata;




10-20

Stop: Clicking it, you can stop collecting the temperature data;

Freeze: Clicking it, you can freeze the displayed curve;

Resume: Clicking it, you can resume the frozen curve.

10.5 Photoelectric

In the Photoelectric tab, the values of photoelectric data and photoelectric datacurve of the selected wavelength are displayed.

Figure 10-2 Photoelectric tab

Display all data

Display curve

Panel

Display maximum data

of each wavelengthDisplay minimum data

of each wavelength

Display difference

between the above two

Through the panel, you can set relevant parameters of photoelectric test data andphotoelectric curve.

Nine wavelengths (340, 405, 450, 510, 546, 578, 630, 670 and 700) and a basewavelength are listed on the control panel. You can select one of them to displayits photoelectric data curve.


MinMax Displays maximum and minimum for photoelectric data

of the selected wavelength. YRange Range on the Y-coordinate of the curve. Input upper

limit and lower limit for photoelectric data in the twoboxes.

XRange X-coordinate point of the curve.

CupIndex Specifies the cuvette in certain position of reaction diskto be used to measure photoelectric data.

Dozes-reagent Reagent dispensed to the specified cuvette.

Dozes-sample Sample dispensed to the specified cuvette.

Period Interval for sampling.




10-21


Times The times of sampling.

Mixer stir Select it to stir the reaction mixture; otherwise thereaction mixture will not be stirred.

Reaction disk rotate Select it to enable the reaction disk to rotate duringmeasurement with photoelectric data of allwavelengths displayed; otherwise the reaction disk willnot be rotated and only photoelectric data of theselected wavelength will be displayed.

Through the control panel, you can select parameters like Wavelength, MinMax, YRange, CupIndex and Period. Moreover, you can select Mixer stir as required.

After setting them, click the Confirm button to confirm the settings.

There are 6 more buttons in the panel:

Start: Clicking it, you can start collecting the temperature data;

Stop: Clicking it, you can stop collecting the temperature data;

Freeze: Clicking it, you can freeze the displayed curve;

Resume: Clicking it, you can resume the frozen curve;

Save: Clicking it, you can save the photoelectric data of all current wavelengthsas a .txt file;

Load: Clicking it, you can read the data in a .txt file saved with photoelectricdata.



A-1

Appendix A Boards Connection Diagrams

Reagent

refrigeration

board

A/D conversion

board

Manipulator

connection

board

Power drive

board

Main control

board

Feeder

connection

board

Probes

connection

board

Power supply

assembly



A-5

1 21 2 3 J77J731 2 3J74 1 2 3J71 1 2J751 2 3J72 1 2J76 J87

1 2J88 1 2J81 1 2J79 1 2J80 1 2J78 1 2J82 1 2J83 1 2J84 1J85

1

2

3

4

M37-M38: Fans on sides of lamp

housing

2100-20-08114

1 r e d

2 b l u e

G N D

1 2 V

C a b l e

B A 3 0 - 2 0 - 0 6 5 2 5

M42-M43: Refrigeratio

2100-20-06534

G N D

1 2 V

G N D

C a b l e

B A 3 0 - 2 0 - 0 6 5 2 9

M 3 0

- M 3 1 : P E L T I E R

2

1 0 0 - 2 0 - 0 6 6 3 3

1 red

2 black

3 red

4 black

GND

12V

GND

12V

Cable

BA30-20-06535

J89

COOLER

12V

12V

COOLER

M24-M27: Reaction temperature fan

BA30-21-06530

3 r e d

1 y e l l o w

2 b l u e

C a b l e

B A 3 0 - 2 0 - 0 6 5 2 4

1 b l u e

2 r e d

G N D

1 2 V

C35: Reagent refrigeration

temperature transducer

BA30-21-15175

C a b l e

B A 3 0 - 2 0 - 0 6 5 2 3

M


BA30-30-06312

C a b l e

B A 3 0 - 2 0 - 0 6 4 8 0

2 b l u e

1

r e d

1 r e d

3 y e l l o w

2 b l u e

G N D

1 2 V

F A N

G N D

1 2 V

F A N

3 r e d

1 y e l l o w

2 b l u e

1 r e d

3 y e l l o w

2 b l u e

G N D

1 2 V

F A N

3 r e d

1 y e l l o w

2 b l u e

1 r e d

3 y e l l o w

2 b l u e

G N D

1 2 V

F A N

3 r e d

1 y e l l o w

2 b l u e

1 r e d

3 y e l l o w

2 b l u e

3 2 1 3 2 1 3 2 1 3 2 1

1 b l u e

2 r e d

1 r e d

2 b l u e

G N D

1 2 V

1 b l u e

2 r e d

2 1 2 1

1 b l u e

2 r e d

1

r e d

2

b l u e

1 b l u e

2 r e d

G N D

1 2 V

2

b l u e

1 b l u e

G N D

1 b l k

1 b l k

Fan on rear side

of lamp housing

2 1

1 r e d

2 b l u e



A-7

1 2 3 4 1 2 3 41 21 21 2

2 3 41 6 7 85 2 3 4121 J200J201

J204

J202

J207 J205J206 J203

C a b l e

B A 3 4 - 2 0 - 6 3 6 0 3

2 1

1 2 J 2 2

Power drive board

BA34-30-63559

1 2 J 2 4

2 1

1 2 J 4 5

Main control board

BA34-30-63557

2 1

C a b l e

B A 3 4 - 2 0 - 6 3 6 0 4

C a b l e

B A 3 0 - 2 0 - 1 5 2 9 0

1 2 3 4 J 3 4

2 1 4 3

C a b l e

B A 3 0 - 2 0 - 1 5 2 9 1

1 2 3 4 J 3 5

2 1 4 3

C a b l e

B A 3 0 - 2 0 - 1 5 2 9 2

Probes connection board

BA30-30-15284

C a b l e

B A 3 0 - 2 0 - 1 5 2 9 5

C a b l e

B A 3 0 - 2 0 - 1 5 2 9 6

2 1

b l u e

r e d

b l u e

r e d

b l a c k r e

d

b l u e

g r e e n

w h i t e

r e d

b l u e

g r e e n

w h i t e

r e d

5 g r e e n A G N D

8 r e d 1 2

V

7 b l a c k R P_

L E V E L

6 b l u e R

P_

R A M_

P H O

Sampel level detection board

BA30-30-06304

1 2J66

J65

C32: Sample probe

C a b l e

B A 3 0 - 2 0 - 0 6 5 4 7

P R O B E

G N D

1 g r e e n A G N D

4 r e d 1 2

V

3 b l a c k S P_

L E V E L

2 b l u e S P_

R A M_

P H O

2 3 41

Reagent level detection board

BA30-30-06304

1 2J66

J65

C31: Reagent probe

C a b l e

B A 3 0 - 2 0 - 0 6 5 4 6

P R O B E

G N D

2 3 41

2 1 4 3

2 1

M 2 9 : P r e h

e a t e r

B A 3 0 - 1 0 - 1 5 1 1 5

M 1 9 : D C m i x

i n g m o t o r

2 0 0 0 - 1 0 - 1 5 0 5 2

C 3 0 : P r e h

e a t i n g

t e m p e r a t u r e t r a n s d u c e r

B A 3 0 - 1 0 -

0 6 6 3 1

4 3

2 1 4 3

- +

C a b l e

B A 3 0 - 2 0 - 1 5 2 9 4

1 b l a c k

2 r e d

1 b l a c k

2 r e d

1 b l a c k

2 r e d

1 b l a c k

2 r e d

3

2 b l a c k

3 r e d

2 1 b l u e

r e d

b l u e

r e d

2 1 b l u e

g r e e n

w h i t e

r e d

2 1 4 3 2 1 4 3 b l u e

g r e e n

w h i t e

r e d

r e d

b l a c k

p u r p l e

y e l l o w

o r a n g e

b r o w n



A-8

2

J103

1

4

3

2

J108

1

4

3

2

J102

1

4

3

2

J101

1

4

3

VCC

GND

GND

MAGD_PHO

VCC

GND

GND

VCC

VCC

GND

GND

GND

GND

MAGU_PHO

LOADY_PHO

LOADX_PHO

2

J107

1

9

5 6

3 4

7 8

3 white

1 black

2 blue

4 red

3 white

1 black

2 blue

4 red

3 white

1 green

2 black

4 red

3 white

1 green

2 black

4 red

1 blue

Cable

BA33-21-3

Cable

BA33-20-351

Power drive board

BA34-30-63559

Manipulator connection boardBA30-30-06316

2

J43

1

9

5 6

3 4

7 8

1

10

9

87

65

43

2

1

10

9

87

65

43

2

Main control board

BA34-30-63557

URAM_PHO

LOADX_PHODRAM_PHO

LOADY_PHO

VCCMAGU_PHO

MAGD_PHO

GND

GNDGND

2J109

14 3

G N D

G N D

2J110

14 3

G N D

G N D

2

J106

1

4

3

MAGU+

M A G U -

M A G U +

2 12 1 J105J104

M A G D -

M A G D +

2 red

1 blue

2 red

MAGU-

MAGD+

MAGD-

2 yellow

4 red

3 blue

1 white

URAM_PHO

LOADX_PHO

DRAM_PHO

LOADY_PHO

VCCMAGU_PHO

MAGD_PHO

GND

GNDGND

2

J3

1

4

3

MAGU+

MAGU-

MAGD+

MAGD-

Cable

BA30-20-06463

10

D R A M_

P H O

U R A M_

P H O

Cable

BA33-21-35

Cable

BA33-21-35

Cable

BA33-21-35

Cable

BA33-20-35

Cable

BA30-20-06486

2 yellow

4 red

3 blue

1 white



A-9

2 J96

DC-DC+

12 J97

DC-DC+

1

2J91 14 3

2

J95

1

4

3

2

J94

1

4

3

2

J93

1

4

3

2

J92

1

4

3

VCC

GND

GND

PRESSURE_PHO

VCC

GND

GND

VCC

VCC

GND

GND

GND

GND

LOADE_PHO

MIDDLE_PHO

BACK_PHO

2

J99

1

9

5 6

3 4

7 8

2

1

3 white

1 green

2 black

4 red

3 white

1 green

2 black

4 red

3 white

1 green

2 black

4 red

3 white

1 green

2 black

4 red

3 white

1 green

2 black

4 red

J98

1 black

2 redDC+

DC- M5: Feeder motor

BA30-21-06494

Cable

BA30-20-06490

Cable

BA30-20-06491

Cable

BA30-20-06492

Cable

BA30-20-06489

Cable

BA30-20-06488

Cable

BA30-20-06494Power drive board

BA34-30-63559

DC-

DC+ 2

1

J19

1 blue

2 red

Cable

BA30-20-06464

Feeder connection board

BA30-30-06314

2

J32

1

9

5 6

3 4

7 8

1

109

87

65

43

2

1

109

87

65

43

2

Main control board

BA34-30-63557

PRESSURE_PHOFRONT_PHO

BUTTON_SBACK_PHO

VCCMIDDLE_PHO

LOADE_PHO

GND

GNDGND

PRESSURE_PHO

FRONT_PHOBUTTON_SBACK_PHO

VCCMIDDLE_PHO

LOADE_PHO

GND

GNDGND1010

Cable

BA30-20-06485



Test Points of Boards

B-1

Appendix B Test Points of Boards

B.1 Test Points on A/D Conversion BoardTP TOP/BOTTOM Descriptions

TP1 TOP The signal at 340nm









TP10 TOP The signal of REF

TP11 TOP The start signals of AD conversion

TP12 TOP GND

TP13 TOP The input analog signals of AD conversion

B.2 Test Points on Drive Board

TP TOP/BOTTOM Descriptions

TP1 TOP Signal ground (SHIELD) from the main control board

TP2 TOP 5V and 24V ground (GND)

TP3 TOP 5V and 24V ground (GND)

TP4 TOP 12V ground (12VGND)

B.3 Test Points on Reagent Refrigeration Board

TP TOP/BOTTOM DescriptionsTP1 TOP The temperature switch signal (low)

TP2 TOP The temperature switch signal (high)

TP3 TOP Reference ground

TP4 TOP The switch characteristic of Q1

TP5 TOP The voltage of the temperature transducer

B.4 Test Points on Main Control Board

TP TOP/BOTTOM DescriptionsTP1 BOTTOM&TOP TxD of Sample Unit




B-2


TP2 BOTTOM&TOP RxD of Sample Unit

TP3 BOTTOM&TOP ALE of Sample Unit

TP4 BOTTOM&TOP TxD of photoelectric unit

TP5 BOTTOM&TOP RxD of photoelectric unitTP6 BOTTOM&TOP ALE of photoelectric unit

TP7 BOTTOM&TOP INT of SPI port

TP8 BOTTOM&TOP RxD of Main unit

TP9 BOTTOM&TOP TxD of Main unit

TP10 BOTTOM&TOP ALE of Main unit

TP11 BOTTOM&TOP Clock Output of Crystal 16MHZ

TP12 BOTTOM&TOP TxD of loading/mixing unit

TP13 BOTTOM&TOP RxD of loading/mixing unit

TP14 BOTTOM&TOP ALE of loading/mixing unit

TP15 BOTTOM&TOP GND

TP16 BOTTOM&TOP VDD 3.3V power

TP17 BOTTOM&TOP VEE 1.5V power

TP18 BOTTOM&TOP VTTP +15V power

TP19 BOTTOM&TOP VTTN -15V power

TP20 BOTTOM&TOP GNDTT GND of ±15V

TP21 BOTTOM&TOP VCC 5V Power

TP22 BOTTOM&TOP VPP 12V powerTP23 BOTTOM&TOP VCCA analog power of 5V

TP24 BOTTOM&TOP AGND analog GND of 5V

TP25 BOTTOM&TOP GND

TP26 BOTTOM&TOP RxD of Sample Unit Extended COM for Bar Code Scanner

TP27 BOTTOM&TOP TxD of Sample Unit Extended COM for Bar Code Scanner

TP28 BOTTOM&TOP TxD of Reagent Unit Extended COM for Bar Code Scanner

TP29 BOTTOM&TOP RxD of Main Unit Extended COM for ISE module

TP30 BOTTOM&TOP RxD of Reagent Unit Extended COM for Bar Code Scanner

TP31 BOTTOM&TOP TxD of Main Unit Extended COM for ISE module

TP32 BOTTOM&TOP Clock Output of Crystal 10MHZ

TP33 BOTTOM&TOP ALE of temperature unit

TP34 BOTTOM&TOP TxD of temperature unit

TP35 BOTTOM&TOP RxD of temperature unit

TP36 BOTTOM&TOP RxD of Reagent unit

TP37 BOTTOM&TOP TxD of Reagent unit

TP38 BOTTOM&TOP ALE of Reagent unit




B-3

B.5 Test Points on PFC Board


TP1 BOTTOM The switch waveforms of Q3 and Q4

TP2 BOTTOM The drive waveform of Q5

TP3 BOTTOM The DC output (390V)

TP4 BOTTOM The reference voltage of U2 (7.5V)

TP5 BOTTOM The oscillation waveform of U2 (100KHz)

TP6 BOTTOM The Vss supply (19V)

TP7 BOTTOM The VDD supply (14V)

TP8 BOTTOM The VPP supply (12V)

TP9 BOTTOM The drive waveform of Q12 (85KHz)

TP10 BOTTOM The switch waveform of Q12

TP11 BOTTOM The current waveform of Q12

TP12 BOTTOM The reference voltage of U8 (5V)

TP13 BOTTOM The oscillation waveform of U8

TP14 BOTTOM The 12V(4.5A) supply

B.6 Test Points on 5V/12V Board



TP2 BOTTOM Input side as reference ground

TP3 BOTTOM The VDD supply (14V)



TP6 BOTTOM The switch drive waveform

TP7 BOTTOM The switch waveform

TP8 BOTTOM The 12V(10A) output

TP9 BOTTOM The output control voltage of the board

TP10 BOTTOM The 12V(4.7A) output

TP11 BOTTOM The supply of FAN (12V)

TP12 BOTTOM The 5V output of the board

TP13 BOTTOM Output side as reference ground

TP14 BOTTOM The switch waveform of U355 (72KHz)

TP15 BOTTOM Short circuit with the output side reference ground andmeasure the OVP point of the two 12Vs

TP16 BOTTOM Short circuit with the output side reference ground andmeasure the OVP point of the two 5V




B-4

B.7 Test Points on 24V Board



TP2 BOTTOM Input side as reference ground

TP3 BOTTOM The switch waveform

TP4 BOTTOM The switch drive waveform

TP5 BOTTOM The current waveform of Q402

TP6 BOTTOM The VCC supply (14V)



TP9 BOTTOM The power on/off

TP10 BOTTOM The 24V output

TP11 BOTTOM Output side as reference ground

TP12 BOTTOM The voltage waveforms at the two ends of the rectifier andfly-wheel diode

TP13 BOTTOM Short circuit with the output side reference ground andmeasure the OVP point of the 24V

Mindray BS 300 Analizor Service Manual

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Transcript of Mindray BS 300 Analizor Service Manual