Data SheetProduct Selection Guide

MILLIPLEX™ MAP and Cardiovascular DiseaseLuminex® xMAP® Technology

CRP

MPC

Apo E

sICAM-1

HUMAN

RAT

MOUSE

Your Best Source for the Study of Cardiovascular Disease

CARDIOVASCULAR DISEASE

MILLIPLEX MAP Cardiovascular DiseaseRecognizing the role of inflammatory mechanisms in cardiovascular disease is essential to understanding the correlation between these biomarkers and the pathogenesis of disease.

Millipore provides important tools to understand better the

role of such biomarkers as CRP, PAI-1 and BNP in cardiovascular

disease. Based on the Luminex xMAP platform MILLIPLEX MAP

assists in the investigation of the modulation and expression of

dozens of analytes simultaneously, giving you the advantage of

speed and sensitivity. This product selection guide summarizes

Millipore’s quality multiplex immunoassays designed to assist you

in the study of cardiovascular disease in a variety of species.

52% 17% 7% 6% 4% 14%Coronary Heart

DiseaseStroke Heart

Failure*High Blood Pressure

Diseases of the

Arteries

Other

*Not a true underlying cause

*Not a true underlying cause

Percentage Breakdown of Deaths Due to Cardiovascular Disease in 2004Source: NCHS and NCBLI

52% Coronary Heart Disease

17% Stroke

7% Congestive Heart Failure*

6% High Blood Pressure

4% Diseases of the Arteries

14% Other

52% Coronary Heart Disease

17% Stroke

7% Heart Failure*

6% High Blood Pressure

4% Diseases of the Arteries

14% Other

Percentage Breakdown of Deaths Due to Cardiovascular Disease in 2004Source: NCHS and NCBLI

Rat CVD • Panels 1, 2 and 3

Rat/Mouse CVD • CRP Single-Plex

Apolipoprotein • Human

Available Panels:

HUMAN RATMOUSE

Human CVD • Panels 1, 2 and 3• Fibrinogen Single-Plex• Haptoglobin Single-plex

Mouse CVD • Panels 1 and 2

The Power of MILLIPLEX MAP Multiplex Assays

A study by Nancy J. Brown of the Department of

Cardiac Surgery at Vanderbilt University School of

Medicine in Nashville, TN, investigated the hypothesis

that biomarkers could predict the development

of postoperative atrial fibrillation. Using Millipore

MILLIPLEX MAP multiplex panels, blood samples from

253 adult patients were screened for 21 biomarkers

immediately after cardiopulmonary bypass surgery.

PAI-1, IL-6, and NT-proBNP concentrations were

significantly higher in the blood of patients who

developed postoperative atrial fibrillation, as shown

in the graph at right. The study demonstrated that

an elevated preoperative or postoperative PAI-1

antigen concentration is an independent predictor

for the development of atrial fibrillation after

cardiopulmonary bypass surgery.

Pretorius M, Donahue BS, Yu C, Grelish JP, Roden DM,

Brown NJ. Plasminogen activator inhibitor-1 as a predictor

of postoperative atrial fibrillation after cardiopulmonary

bypass. Circulation. 2007 Sep 11;116(11 Suppl):I1-7.

0

100

200

300

400

500

600

700

800

900

PAI-1 IL-6 MCP-1 NT-proBNP

Con

cent

rati

on (pg

/mL,

ng/

mL

for

PAI-

1)

Elevated PAI-1 Antigen as Predictor of Atrial Fibrillation following Cardiac Bypass Surgery

No Artrial FibrillationArtrial Fibrillation

MILLIPLEX MAP Cardiovascular (CVD) PanelsCardiovascular disease (CVD), particularly atherosclerotic vascular disease,

is a leading cause of global mortality with an estimated 17.5 million CVD related

deaths in 2005, accounting for 30% of all global deaths (WHO). Now widely recognized,

inflammatory mechanisms play a vital role in the initiation, maintenance and progression

of vascular disease with a strong correlation between inflammatory markers and prognosis

in acute and chronic coronary artery disease. In addition, numerous studies have demonstrated

an association of obesity and diabetes with cardiovascular risk factors.

Biomarkers that may indicate a greater risk for heart disease include: • Higher Fibrinogen blood levels

• Higher Plasminogen Activator Inhibitor 1 (PAI-1) blood levels

• High inflammation as measured by C Reactive Protein (CRP)

• Elevated blood levels of B-type natriuretic peptide (BNP) or N-terminal portion of proBNP (NT-proBNP).

AVAILABLE ANALyTES Hum

an

CV

D1

Hum

an

CV

D2

Hum

an

CV

D3

Hum

an

Sin

gle

Ple

x

Mou

se

CV

D1

Mou

se

CV

D2

Rat

CV

D1

Rat

CV

D2

Rat

CV

D3

Rat

/Mou

se

Sin

gle

Ple

x

Adiponectin • • •Apolipoprotein A1 (Apo A1) •

Apolipoprotein E (Apo E) •B-type Natriuretic Peptide (BNP) •

N-terminal Portion of ProBNP (NT-proBNP) •C Reactive Protein (CRP) • •

Soluble E-Selectin (sE-Selectin) • • •Fibrinogen • • • •

Haptoglobin •Soluble Intercellular Adhesion Molecule-1 (sICAM-1) • • •

Interferon gamma (IFNg) •Interleukin-1 beta (IL-1b) •

Interleukin-6 (IL-6) • •Interleukin-8 (IL-8) •

Interleukin-10 (IL-10) •Monocyte chemotactic protein-1 (MCP-1) • •

Matrix Metalloproteinase-9 (MMP-9) • •Myeloperoxidase (MPO) • •

Total Plasminogen Activator Inhibitor-1 (PAI-1 total) • • •Serum Amyloid A •Serum Amyloid P •

Tissue inhibitor of metalloproteinase 1 (TIMP-1) •Troponin I •

Troponin T •Tumor Necrosis Factor alpha (TNFa) • •

Soluble Vascular Cell Adhesion Molecule-1 (sVCAM-1) • •Vascular Endothelial Growth Factor (VEGF) • •

Von Willebrand Factor (vWF) •

100,000Human CVD1 Panel

Standard Curves

10,000

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Concentration (pg/mL)

100,000Human CVD3 Panel

Standard Curves

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100,000Human CVD2 Panel

Standard Curves

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Concentration (ng/mL)

100,000Fibrinogen Single Plex

Standard Curve

10,000

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Concentration (ng/mL)

100,000Haptoglobin Single Plex

Standard Curve

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Concentration (ng/mL)

AdiponectinsE-SelectinsICAM-1sVCAM-1MMP-9MPOPAI-1 (total)

FibrinogenCRPSAASAP

IL-1βIL-6IL-8IL-10IFNγTNFαMCP-1VEGFNT-proBNP

AVAILABLE ANALyTES Hum

an

CV

D1

Hum

an

CV

D2

Hum

an

CV

D3

Hum

an

Sin

gle

Ple

x

Mou

se

CV

D1

Mou

se

CV

D2

Rat

CV

D1

Rat

CV

D2

Rat

CV

D3

Rat

/Mou

se

Sin

gle

Ple

x

Adiponectin • • •Apolipoprotein A1 (Apo A1) •

Apolipoprotein E (Apo E) •B-type Natriuretic Peptide (BNP) •

N-terminal Portion of ProBNP (NT-proBNP) •C Reactive Protein (CRP) • •

Soluble E-Selectin (sE-Selectin) • • •Fibrinogen • • • •

Haptoglobin •Soluble Intercellular Adhesion Molecule-1 (sICAM-1) • • •

Interferon gamma (IFNg) •Interleukin-1 beta (IL-1b) •

Interleukin-6 (IL-6) • •Interleukin-8 (IL-8) •

Interleukin-10 (IL-10) •Monocyte chemotactic protein-1 (MCP-1) • •

Matrix Metalloproteinase-9 (MMP-9) • •Myeloperoxidase (MPO) • •

Total Plasminogen Activator Inhibitor-1 (PAI-1 total) • • •Serum Amyloid A •Serum Amyloid P •

Tissue inhibitor of metalloproteinase 1 (TIMP-1) •Troponin I •

Troponin T •Tumor Necrosis Factor alpha (TNFa) • •

Soluble Vascular Cell Adhesion Molecule-1 (sVCAM-1) • •Vascular Endothelial Growth Factor (VEGF) • •

Von Willebrand Factor (vWF) •

Human Cardiovascular (CVD) Panels

Specificity

Cross-reactivity between the antibodies and any of the

other analytes in this panel is non-detectable or negligible.

Assay Conditions

CVD1 Panel

• This kit may be used for the analysis of all or any

combination of the analytes in this panel in serum,

plasma, other body fluids, and cell/tissue extract or

culture samples.

• A 1:100 dilution (or 1:50 if adiponectin is not measured)

is required for both serum and plasma samples.

• This is an overnight assay requiring 25 µL sample size.

CVD2 Panel and Fibrinogen Single Plex

• These kits may be used for the analysis of all or any

combination of the analytes in these panels in serum,

plasma, other body fluids, and cell/tissue extract or

culture samples.

• A 1:1,000 to 1:5,000 dilution (actual dilution factor is

sample dependent) is required for serum samples.

• A 1:1,000 to 1:5,000 dilution (actual dilution factor is

sample dependent) except Fibrinogen (use 1:40,000) in

plasma samples.

• These are 2-hour assays requiring 25 µL sample size.

CVD3 Panel

• This kit may be used for the analysis of all or any

combination of the analytes in this panel in serum,

plasma, other body fluids, and cell/tissue extract or

culture samples.

• No dilutions are required for serum or plasma samples.

• This is a 2-hour assay (or overnight incubation for

increased sensitivity) requiring 25 µL sample size.

PERfORMANCE SPECIfICATIONS

HUMAN

HUMAN CARDIOVASCULAR (CVD) PANELS – PERfORMANCE SPECIfICATIONS

HUMAN

AnalyteStandard Curve Range (pg/mL) Sensitivity (pg/mL)

CVD1 CVD2 CVD3 Single Plex CVD1 CVD2 CVD3 Single Plex

Adiponectin 80 – 250,000 – – – 56 – – –

NT–proBNP – – 3.2 – 5,000 – – – 11.5 –

CRP – 80 – 250,000 – – – 6 – –

sE-Selectin 80 – 250,000 – – – 79 – – –

Fibrinogen – 80 – 250,000 – 80 – 250,000 – 12 – 12

Haptoglobin – – – 80 – 250,000 – – – 52

sICAM-1 80 – 250,000 – – – 9 – – –

IFNg – – 0.64 – 10,000 – – – 0.4 –

IL-1b – – 0.64 – 10,000 – – – 0.3 –

IL-6 – – 0.64 – 10,000 – – – 0.7 –

IL-8 – – 0.64 – 10,000 – – – 0.5 –

IL-10 – – 0.64 – 10,000 – – – 0.3 –

MCP-1 – – 0.64 – 10,000 – – – 0.6 –

MMP-9 16 – 50,000 – – – 1 – – –

MPO 16 – 50,000 – – – 7 – – –

PAI-1 (total) 16 – 50,000 – – – 1 – – –

Serum Amyloid A – 80 – 250,000 – – – 210 – –

Serum Amyloid P – 80 – 250,000 – – – 55 – –

TNFa – – 0.64 – 10,000 – – – 0.2 –

sVCAM-1 80 – 250,000 – – – 16 – – –

VEGF – – 0.64 – 10,000 – – – 1.5 –

100,000Human CVD1 Panel

Standard Curves

10,000

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100,000Human CVD3 Panel

Standard Curves

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100,000Human CVD2 Panel

Standard Curves

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Concentration (ng/mL)

100,000Fibrinogen Single Plex

Standard Curve

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Concentration (ng/mL)

100,000Haptoglobin Single Plex

Standard Curve

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Concentration (ng/mL)

AdiponectinsE-SelectinsICAM-1sVCAM-1MMP-9MPOPAI-1 (total)

FibrinogenCRPSAASAP

IL-1βIL-6IL-8IL-10IFNγTNFαMCP-1VEGFNT-proBNP

Haptoglobin Single Plex

• This kit may be used for the analysis of all or any

combination of the analytes in this panel in serum,

plasma, other body fluids, and cell/tissue extract or

culture samples.

• A 1:40,000 dilution is required for both serum and

plasma samples.

• This is a 2-hour assay requiring 25 µL sample size.

AnalyteAccuracy (%) Precision Intra / Inter (%CV)

CVD1 CVD2 CVD3 Single Plex CVD1 CVD2 CVD3 Single Plex

Adiponectin 96.4 – – – 9.2 / 15.9 – – –

NT–proBNP – – 102.6 – – – 8.3 / 8.4 –

CRP – 121.0 – – – 8.0 / 17.5 – –

sE-Selectin 104.1 – – – 11.2 / 13.4 3.7 / 16.9 – 3.7 / 16.9

Fibrinogen – 95.6 – 95.6 – – – 4.4 / 21.0

Haptoglobin – – – 93.5 – – – –

sICAM-1 83.0 – – – 7.9 / 9.7 – – –

IFNg – – 101.3 – – – 6.1 / 10.3 –

IL-1b – – 99.6 – – – 9.8 / 8.7 –

IL-6 – – 104.8 – – – 9.0 / 10.4 –

IL-8 – – 101.2 – – – 11.4 / 9.0 –

IL-10 – – 97.9 – – – 8.3 / 11.2 –

MCP-1 – – 96.5 – – – 11.3 / 13.7 –

MMP-9 91.1 – – – 6.8 / 11.7 – – –

MPO 91.7 – – – 12.3 / 16.3 – – –

PAI-1 (total) 84.6 – – – 11.8 / 12.5 – – –

Serum Amyloid A – 97.1 – – – 3.8 / 19.8 – –

Serum Amyloid P – 108.3 – – – 13.4 / 20.7 – –

TNFa – – 96.7 – – – 10.0 / 9.4 –

sVCAM-1 103.9 – – – 4.5 / 8.5 – – –

VEGF – – 99.2 – – – 8.2 / 6.9 –

HUMAN

MOUSE

Mouse Cardiovascular (CVD) Panels

Specificity

Cross-reactivity between the antibodies and any of the

other analytes in this panel is non-detectable or negligible.

Assay Conditions

CVD1 Panel

• This kit may be used for the analysis of all or any

combination of the analytes in this panel in serum,

plasma, other body fluids, and cell/tissue extract or

culture samples.

• A 1:100 dilution is required for both serum and plasma

samples.

• This is an overnight assay requiring 25 µL sample size.

CVD2 Panel

• These kits may be used for the analysis of all or any

combination of the analytes in these panels in serum,

plasma, other body fluids, and cell/tissue extract or

culture samples.

• A 1:5,000 dilution is required for both serum and plasma

samples.

• These are overnight assays requiring 25 µL sample size.

PERfORMANCE SPECIfICATIONS

100,000Mouse CVD2 Panel

Standard Curves

10,000

1,000

100

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100

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10,000

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Concentration (pg/mL)

100,000Mouse CVD1 Panel

Standard Curves

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Concentration (ng/mL)

Apo A1

Apo E

Fibrinogen

Adiponectin

sE-Selectin

MMP-9

sICAM-1

sVCAM-1

PAI-1 (total)

MOUSE CARDIOVASCULAR (CVD) PANELS – PERfORMANCE SPECIfICATIONS

MOUSE

AnalyteStandard Curve Range (pg/mL) Sensitivity (pg/mL)

CVD1 CVD2 CVD1 CVD2

Adiponectin – 3 – 50,000 – 3

Apo A1 – 32 – 500,000 – 900

Apo E – 16 – 250,000 – 50

sE-Selectin 0.0064 – 100 – 5.0 –

Fibrinogen – 320 – 5,000,000 – 310

sICAM-1 0.0064 – 100 – 3.0 –

MMP-9 0.0064 – 100 – 4.0 –

PAI-1 (total) 0.0064 – 100 – 2.0 –

sVCAM-1 0.0064 – 100 – 2.0 –

Analyte

Accuracy (%) Precision (%CV)

CVD1 CVD2 Intra–Assay CVD1

Inter–Assay CVD1

Intra–Assay CVD2

Inter–Assay CVD2

Adiponectin – 98.4 – – 7.3 13.4

Apo A1 – 104.8 – – 12.1 8.2

Apo E – 92.1 – – 12.3 11.0

sE-Selectin 82.0 – 4.5 5.7 – –

Fibrinogen – 75.7 – – 12.1 12.1

sICAM-1 95.3 – 3.1 4.4 – –

MMP-9 90.0 – 3.2 6.0 – –

PAI-1 (total) 63.3 – 4.7 6.1 – –

sVCAM-1 76.6 – 5.4 6.1 – –

Rat Cardiovascular (CVD) Panels

Specificity

Cross-reactivity between the antibodies and any of the

other analytes in this panel is non-detectable or negligible.

Assay Conditions

CVD1 Panel

• This kit may be used for the analysis of all or any

combination of the analytes in this panel in serum,

plasma, other body fluids, and cell/tissue extract or

culture samples.

• Generally, serum or plasma samples from normal

subjects should be diluted 1:4 using the Assay Buffer

provided in the kit as the sample diluent. If dilution of

serum/plasma samples is beyond 1:4, please use diluted

serum matrix provided in the kit for further dilution of

samples.

• This is an overnight assay requiring 25 µL sample size.

CVD2 Panel

• This kit may be used for the analysis of all or any

combination of the analytes in this panel in serum,

plasma, other body fluids, and cell/tissue extract or

culture samples.

• Generally, serum or plasma samples from normal

subjects should be diluted 1:100 using the Assay Buffer

provided in the kit as the sample diluent.

• This is an overnight assay requiring 25 µL sample size.

CVD3 Panel

• This kit may be used for the analysis of all or any

combination of the analytes in this panel in serum,

plasma, other body fluids, and cell/tissue extract or

culture samples.

• Generally, serum or plasma samples from normal

subjects should be diluted 1:1,000 using the Assay

Buffer provided in the kit as the sample diluent.

• This is an overnight assay requiring 25 µL sample size.

PERfORMANCE SPECIfICATIONS

RAT

Rat CVD1 PanelStandard Curves

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Concentration(ng/mL for MPO and vWF, pg/mL for others)

BNPMCP-1TnlMPOIL-6vWFTIMP-1TnTTNF-alphaPAI-1 (total)VEGF

Rat CVD2 PanelStandard Curves

100,000

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sICAM-1sE-selectin

Rat CVD3 PanelStandard Curves

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FibrinogenAdiponectin

RAT

RAT CARDIOVASCULAR (CVD) PANELS – PERfORMANCE SPECIfICATIONS

Analyte

Standard Curve Range (pg/mL)

Sensitivity (pg/mL)

CVD1 CVD2 CVD3 CVD1 CVD2 CVD3

Adiponectin – – 130 – 2,000,000 – – 76.0

BNP 14 – 10,000 – – 4.2 – –

CRP – – – – – –

sE-Selectin – 27 – 20,000 – – 3.4 –

Fibrinogen – – 640 – 10,000,000 – – 980.0

sICAM-1 – 27 – 20,000 – – 6.0 –

IL-6 14 – 10,000 – – 2.8 – –

MCP-1 137 – 100,000 – – 133.6 – –

MPO 3,000 – 2,000,000 – – 380.0 – –

PAI-1 (total) 34 – 25,000 – – 3.0 – –

TIMP-1 – – – 157.4 – –

TNFa 34 – 25,000 – – 12.8 – –

Troponin I 137 – 100,000 – – 322.8 – –

Troponin T 137 – 100,000 – – 24.2 – –

VEGF 14 – 10,000 – – 19.4 – –

Von Willebrand Factor 3,000 – 2,000,000 – – 880.0 – –

Analyte

Accuracy (%) Serum

Accuracy (%) Plasma

Precision (%CV) Intra-Assay

Precision (%CV) Inter Assay

CVD1 CVD2 CVD3 CVD1 CVD2 CVD3 CVD1 CVD2 CVD3 CVD1 CVD2 CVD3

Adiponectin – – 82.6 – – 104.9 – – 8.2 – – 4.2

BNP 100.8 – – 117.9 – – 11.2 – – 6.1 – –

CRP – – – – – – – – – – – –

sE-Selectin – 91.4 – – 79.6 – – 5.0 – – 8.0 –

Fibrinogen – – 151.3 – – 138.8 – – 16.7 – – 22.6

sICAM-1 – 96.1 – – 95.2 – – 3.3 – – 9.7 –

IL-6 100.1 – – 126.1 – – 1.3 – – 5.2 – –

MCP-1 103.1 – – 128.8 – – 7.3 – – 4.5 – –

MPO 100.8 – – 130.1 – – 11.8 – – 32.3 – –

PAI-1 (total) 92.8 – – 124.4 – – 11.6 – – 4.5 – –

TIMP-1 93.0 – – 146.2 – – 6.9 – – 7.8 – –

TNFa 101.4 – – 112.1 – – 7.3 – – 5.4 – –

Troponin I 98.0 – – 165.9 – – 9.6 – – 21.0 – –

Troponin T 100.5 – – 203.2 – – 8.5 – – 22.3 – –

VEGF 110.6 – – 116.3 – – 8.9 – – 10.7 – –

Von Willebrand Factor 99.6 – – 81.0 – – 13.0 – – 5.3 – –

AVAILABLE ANALyTES A

polip

opro

tein

A-I

Apo

lipop

rote

in A

-II

Apo

lipop

rote

in B

Apo

lipop

rote

in C

-II

Apo

lipop

rote

in C

-III

Apo

lipop

rote

in E

Human • • • • • •

Human Apo AIV and Apo AV are available in an ELISA format.

RAT/MOUSE

Rat/Mouse CRP Single Plex

Standard Curve Range

0.0064 to 100 ng/mL

Sensitivity

5.7 pg/mL

Accuracy

99.6% for rat samples

82.3% for mouse samples

Precision

Intra-assay: < 10.0%

Inter-assay: < 15%

Specificity

Cross-reactivity between the antibodies and any of the

other analytes in this panel is non-detectable or negligible.

Assay Conditions

• This kit may be used for the analysis of all or any

combination of the analytes in this panel in serum,

plasma, other body fluids, and cell/tissue extract or

culture samples.

• A 1:200 dilution is required for normal mouse serum/

plasma samples.

• A >1:40,000 dilution for normal rat serum/plasma

samples.

• This is an overnight assay requiring 25 µL sample size.

PERfORMANCE SPECIfICATIONS

100,000Rat/Mouse CRP Single Plex

Standard Curve

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MILLIPLEX MAP Human Apolipoprotein Multiplex Panels

Formed predominantly in the liver and

intestine, apolipoproteins play an essential

role in the production and transport of

cholesterol around the body. There are at

least nine types of apolipoproteins, and

each type binds either HDL or LDL and

other lipoproteins in the blood. Millipore’s

MILLIPLEX MAP Human Apolipoprotein Panel

measures six of these important biomarkers

for use in research to better define risk for

coronary heart disease, metabolic syndrome,

diabetes or stroke.

Human Apolipoprotein Panel

Calibration Curve Ranges

Varies with specific apolipoprotein

Specificity

To test cross-reactivity among the assays in the panel,

individual apolipoprotein standards were prepared at a

concentration four times greater than the highest

concentration in the calibration curve, then tested in

the 6-plex assays with multiplexed beads containing

immobilized capture antibodies and individual detection

antibodies. There was negligible cross-reactivity within

the panel.

PERfORMANCE SPECIfICATIONS

Analyte Sensitivity (ng/mL)

Precision (%CV)Accuracy

(%)Intra-Assay

Inter-Assay

Apo A-I 0.5 5 11 93.0

Apo A-II 0.5 5 13 85.3

Apo B 3.0 8 14 95.3

Apo C-II 0.3 5 15 91.0

Apo C-III 0.1 5 13 100.0

Apo E 0.1 5 22 92.2

HUMAN

Assay Conditions

• This kit may be used for the analysis of all or any

combination of the analytes in this panel in serum,

plasma, other body fluids, and cell/tissue extract or

culture samples.

• We recommend that human serum and plasma samples

be diluted 10,000-fold using Assay Buffer as the diluent.

Samples can be diluted using a two-step protocol:

– Step 1, add 4 µL serum or plasma to

396 µL Assay Buffer (i.e. 100-fold).

– Step 2, add 4 µL of the 100-fold diluted sample

from Step 1 to another microfuge tube containing

396 µL Assay Buffer.

– Users may make similar dilutions using less

sample volume to conserve Assay Buffer.

• This is a 2-hour assay requiring 10 µL of serum or

plasma or 25 µL of tissue culture sample.

14,000Human Apo A-IStandard Curve

10,000

2,000

00 1 10 10

0

10,000

100,00

0

Med

ian

Fluo

resc

ence

Inte

nsit

y (M

FI)

Concentration (ng/mL)1,00

0

12,000

4,000

6,000

8,000

5,000Human Apo C-IIStandard Curve

3,000

0

0.1

1.0 10 10

0

10,000

Med

ian

Fluo

resc

ence

Inte

nsit

y (M

FI)

Concentration (ng/mL)1,00

0

4,000

1,000

2,000

16,000

14,000

Human Apo A-IIStandard Curve

10,000

2,000

00 1 10 10

0

10,000

100,00

0

Med

ian

Fluo

resc

ence

Inte

nsit

y (M

FI)

Concentration (ng/mL)1,00

0

12,000

4,000

6,000

8,000

14,000Human Apo C-IIIStandard Curve

10,000

2,000

00 1 10 10

0

10,000

Med

ian

Fluo

resc

ence

Inte

nsit

y (M

FI)

Concentration (ng/mL)1,00

0

12,000

4,000

6,000

8,000

10,000Human Apo BStandard Curve

10

00 1 10 10

0

10,000

100,00

0

Med

ian

Fluo

resc

ence

Inte

nsit

y (M

FI)

Concentration (ng/mL)1,00

0

100

1,000

18,000Human Apo EStandard Curve

10,000

2,000

00 1 10 10

0

10,000

Med

ian

Fluo

resc

ence

Inte

nsit

y (M

FI)

Concentration (ng/mL)1,00

0

14,000

16,000

12,000

4,000

6,000

8,000

xMAP TECHNOLOGy PROCESS fLOw

with xMAP technology, you will benefit from

the ability to simultaneously quantitate up to

100 analytes in a single sample, using as little

as 25 µL or less.

• Speed/High-Throughput: Simultaneously measure

the concentration of a large number of analytes,

allowing you to do more faster, gaining early and

comprehensive data so critical to your work.

• Accuracy: xMAP Technology generates real-time

analysis and accurate quantification of biological

interactions.

• Reproducibility: The high-volume production of xMAP

beads allows assay standardization that solid-phased

flat arrays cannot provide.

COMPARISON Of THE xMAP & ELISA TECHNOLOGIESSide-by-side comparison of Multiplexing vs. ELISAs (Study of 10 different analytes in 80 test samples).

xMAP Technology ELISA

Number of plates required 1 10

Results per plate 800 80

Total sample used per panel 50 µL 4 mL

Internal controls possible Yes No

Dynamic Range 1 – 10,000 pg/mL 10 – 2500 pg/mL

Lower limit of detection ~1 pg/mL ~1 pg/mL

Luminex xMAP Technology

1 Luminex uses proprietary techniques to internally color-code microspheres with two fluorescent dyes. Through precise concentrations of these dyes, 100 distinctly colored bead sets can be created, each of which is coated with a specific capture antibody.

3 The microspheres are allowed to pass rapidly through a laser, which excites the internal dyes marking the microsphere set. A second laser excites PE, the fluorescent dye on the reporter molecule.

2 After an analyte from a test sample is captured by the bead, a biotinylated detection antibody is introduced. The reaction mixture is then incubated with Streptavidin PE conjugate, the reporter molecule, to complete the reaction on the surface of each microsphere.

Bead #12, IL-6Bead #29, IFN-γ

Bead #56, TNF-α

COMPARISON Of THE xMAP & ELISA TECHNOLOGIESSide-by-side comparison of Multiplexing vs. ELISAs (Study of 10 different analytes in 80 test samples).

xMAP Technology ELISA

Number of plates required 1 10

Results per plate 800 80

Total sample used per panel 50 µL 4 mL

Internal controls possible Yes No

Dynamic Range 1 – 10,000 pg/mL 10 – 2500 pg/mL

Lower limit of detection ~1 pg/mL ~1 pg/mL

PRODUCTS • Higher Quality – Industry-leading QC, QA and Six Sigma Principles

• More Efficient – Analytically validated panels for consistency and reproducibility

• All-In-One Kit – A single kit with a single catalog number

• More Flexible – Select only the analytes needed or choose a pre-mixed kit

• Continuity of Knowledge Is the Key – Millipore “owns” every step of the process,

from R&D to manufacturing

• More Choices – The broadest range of multiplex analytes for the Luminex xMAP platform

INSTRUMENTS • Speed/High-Throughput – Simultaneously measure the concentration of a large number

of different analytes, allowing you to do more faster, gaining early and comprehensive

data so critical to your work.

• Accuracy – xMAP technology generates real-time analysis and accurate quantification of

the biological interactions.

• Reproducibility – High-volume production of xMAP microspheres allows assay

standardization that solid-phased flat arrays cannot provide.

• Low Sample Volume – With minimal hands-on time, you can screen more than

40 analytes in a single sample using as little as 25 µL or less.

SOfTwARE • The Luminex xPONENT® software helps you manage and track all of your multiplex

assays by combining ease of use and flexibility to perform both protein and nucleic acid

testing on one system. It’s also compatible with the upcoming magnetic bead (MagPlex™

beads) assays, and will be moving forward with compatible future instrument platforms.

• NEw! Millipore now offers MILLIPLEX Analyst software, developed exclusively for

Millipore by VigeneTech. This data analysis package helps you do your best work with

increased speed and efficiency. MILLIPLEX Analyst software provides a whole new level

of data analysis by allowing you to import large data sets and benefit from the ability to

calculate your results in seconds. The user-friendly plate setup feature lets you work

with speed and efficiency while the unique algorithms on even the most challenging data

sets give you the performance you need for multiplexing analysis.

SERVICES • Millipore’s BioMarker Services (BMS) has been performing immunoassays for over

25 years.

• Assay services are available for RIAs, ELISAs, IRMAs and multiplex assays, using both

Millipore kits as well as any commercially available assays.

• Quality control, reproducible results, and confidentiality are of paramount importance

to our BMS team.

• Millipore’s Regulatory Compliant Laboratory is uniquely positioned to provide

bioanalytical services to support your preclinical and clinical studies.

MILLIPLEX MAP: Everything You Need – All In One Place

MILLIPLEX MAP

MILLIPLEX Analyzer

MILLIPLEX Analyst

BioPharma Services

For a complete and up-to-date list of MILLIPLEX MAP analytes, visit www.millipore.com/analytes.

TO PLACE AN ORDER Visit www.millipore.com/milliplex or call 1-800-MILLIPORE.

fOR PRODUCT OR APPLICATION INfORMATION Contact Technical Services at US Toll Free 1-866-441-8400,

1-636-441-8400, or www.millipore.com/techservice.

TO REQUEST A SALES REP Email: drugdiscovery@millipore.com.

Millipore is a registered trademark of Millipore Corporation. The M mark, Advancing Life Science Together, and MILLIPLEX are trademarks of Millipore Corporation.Luminex, xPONENT, and xMAP are registered trademarks of Luminex Corporation. MagPlex is a trademark of Luminex Corporation. Lit. No. PB2277EN00 08DD013 Printed in U.S.A. 11/08 DD-SBU-08-01090© 2008 Millipore Corporation, Billerica, MA 01821 U.S.A. All rights reserved.

Description Quantity Catalogue No.

Human Human CVD1 Biomarker Panel 96-well plate HCVD1-67AK

Human CVD2 Biomarker Panel (Acute Phase Proteins) 96-well plate HDVD2-67BK-03

Human CVD3 Biomarker Panel 96-well plate HCVD3-67CK

Human CVD Fibrinogen Single-Plex 96-well plate HCVD2-67BK-1FBN

Human CVD Haptoglobin Single-Plex 96-well plate HCVD2-67BK-1HAP

Human Apolipoprotein Panel 96-well plate APO-69K

Mouse Mouse CVD1 Biomarker Panel 96-well plate MCVD1-77AK

Mouse CVD2 Biomarker Panel 96-well plate MCVD277BK

Rat/Mouse Rat/Mouse CVD CRP Single-Plex 96-well plate MCVD77K1CRP

Rat Rat CVD1 Biomarker Panel 96-well plate RCVD1-89K

Rat CVD2 Biomarker Panel 96-well plate RCVD2-89K

Rat CVD3 Biomarker Panel 96-well plate RCVD3-89K

ORDERING INfORMATION

o A variety of RIAs and ELISAs for the study of

Metabolic Syndrome in many species –

human, mouse, rat, canine, porcine, bovine

o Human Apo AIV ELISA

o Human Apo AV ELISA

o Adipokine / Adipocyte Multiplex Panels –

human, mouse, rat, canine

o Adiponectin Single Plex Panels – mouse, rat

o Gut Hormone Multiplex Panels – human, mouse, rat

o Endocrine Multiplex Panels – human, mouse, rat, canine

o Pituitary Multiplex Panels – human, rat

o Rat Stress Hormone Multiplex Panel

o Rat Thyroid Multiplex Panels

o Rat/Mouse IGF-1 Single Plex

o Cytokine / Chemokine Multiplex Panels –

human, high sensitivity human, non-human

primate, mouse, rat, canine

VigeneTech

www.millipore.com/offices

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