![CONFOCAL WHITE LIGHT MICROSCOPY - oetg.at€¦ · Confocal microscopy, as first described by M. Minski in 1957 (originally named “double focusing microscopy”) [1, 2] becomes a](https://static.fdocuments.in/doc/165x107/600636c9a19b977153619a36/confocal-white-light-microscopy-oetgat-confocal-microscopy-as-first-described.jpg)
Microscopy As
27
SFX GREENHERALD INTERNATIONAL SCHOOL BIOLOGY MCQ TEST FOR AS LEVEL Chapters: Microscopy Name: Roll No. Time: 20 minutes Date: Marks: 20 Marks scored out of 20 Subject Teacher’s Signature (For MCQs Place a CIRCLE over the alphabet of the corresponding correct answer) 1. What describes resolution in microscopy? A the ability to distinguish between two objects that are very close together B the clarity of the image formed by the microscope C the number of times the image has been magnified by the objective lens D the power of the microscope to focus on very small objects 2. What is compound microscope? A Microscope with the capability to view oil immersion B Microscope with the capability to view compounds C Microscope with a single lens D Microscope with two lenses 3. What is the total magnification achieved with a compound microscope? A Magnification of objective lens B Magnification of ocular lens C Magnification of ocular lens added to the magnification of the objective lens
-
Upload
emrul-hasan-emran -
Category
Documents
-
view
481 -
download
0
Transcript of Microscopy As
SFX GREENHERALD INTERNATIONAL SCHOOL
BIOLOGY MCQ TEST FOR AS LEVEL
Chapters: Microscopy
Name: Roll No.
Time: 20 minutes Date: Marks: 20
Marks scored out of 20 Subject Teacher’s Signature
(For MCQs Place a CIRCLE over the alphabet of the corresponding correct answer)
1. What describes resolution in microscopy?
A the ability to distinguish between two objects that are very close together
B the clarity of the image formed by the microscope
C the number of times the image has been magnified by the objective lens
D the power of the microscope to focus on very small objects
2. What is compound microscope?
A Microscope with the capability to view oil immersion
B Microscope with the capability to view compounds
C Microscope with a single lens
D Microscope with two lenses
3. What is the total magnification achieved with a compound microscope?
A Magnification of objective lens
B Magnification of ocular lens
C Magnification of ocular lens added to the magnification of the objective lens
D Magnification of ocular lens multiplied by the magnification of the objective lens
4. What is another name for the light microscope?
A Simple microscope
B Compound microscope
C Phase contrast microscope
D Dissection microscope
5. Which microscope would be particularly useful for looking at living cells?
A Simple microscope
B Compound microscope
C Phase contrast microscope
D Transmission electron microscope
6. Which type of microscope has only one lens?
A Simple microscope
B Compound microscope
C Phase contrast microscope
D Dissection microscope
7. What is the resolution, in nanometres, of an electron microscope and of a light microscope?
electron light
microscope microscope
A 0.5 20
B 0.5 200
C 5.0 20
D 5.0 200
8. What describes the features of an electron microscope?
maximum
magnification resolution / nm specimen
A 2.5 ×103 2.5 ×102 dead
B 2.5 ×104 5.0 ×10-1 living
C 2.5 ×105 5.0 ×10-1 dead
D 5.0 ×105 2.5 ×102 living
9. Which cell structure can be seen only with an electron microscope?
A cell surface membrane
B cell wall
C chromosome
D nucleolus
10. The diagram shows a graduated slide, with divisions of 0.1 mm viewed using an eyepiece graticule.
Pollen grains were grown in a sugar solution and viewed using the eyepiece graticule. Diagram 1
shows the pollen grains at first and diagram 2 shows them after four hours.
diagram 1 diagram 2
at start after 4 hours
What is the growth rate of the pollen tubes?
A 5 µmh-1
B 10 µmh-1
C 5 mmh-1
D 10 mmh-1
11. A specimen is viewed under a microscope using green light with a wavelength of 510 nm. If the same
specimen is viewed under the same conditions, but using red light with a wavelength of 650 nm
instead, what effect will this have on the magnification and on the resolution of the microscope?
magnification resolution
A decreased decreased
B increased increased
C remains the same increased
D remains the same decreased
12. The diagram below is drawn from an electron micrograph of an animal cell.
Which represents the same cell, seen under a light (optical) microscope at ×400 magnification?
C
13. The diagram shows a photomicrograph. Its magnification is ×2800.
What is the diameter of the nucleolus?
A 2.5 µm
B 5 µm
C 10 µm
D 20 µm
14. The diagram shows a stage micrometer on which the small divisions are 0.1 mm. It is viewed through
an eyepiece containing a graticule.
The stage micrometer is replaced by a slide of a plant cell.
What is the width of a chloroplast?
A 5 μm
B 10 μm
C 50 μm
D 100 μm
15. A student is asked to study two photographs, taken at the same magnification, of a palisade mesophyll
cell, one using a high quality light microscope and the other using an electron microscope.
The student observed
1 the cisternae of the Golgi apparatus
2 the grana in the chloroplasts
3 the two membranes of the nuclear envelope
4 the vacuole enclosed by a tonoplast
Which features can be seen because of the higher resolution of the electron microscope?
A 1, 2 and 3
B 1, 2 and 4
C 1, 3 and 4
D 2, 3 and 4
16. Which eyepiece and objective lens combination enables you to see the greatest number of cells in the
field of view?
eyepiece objective
A ×5 ×10
B ×10 ×10
C ×5 ×40
D ×10 ×40
17. The graticule and stage micrometer are used to measure cells. Which is the correct reason why the
graticule calibrated?
A The graticule can be used to make measurements.
B The graticule is magnified by the objective lens.
C The graticule magnifies the specimen.
D The graticule makes comparisons.
18. The diagram shows a stage micrometer, with divisions 0.1 mm apart, viewed through an eyepiece
containing a graticule.
The same eyepiece is now used to examine a blood smear. How many graticule divisions will cover
the diameter of a white cell of 10 µm?
A 1
B 4
C 10
D 20
19. A specimen is viewed under a microscope using green light with a wavelength of 510 nm. If the same
specimen is viewed under the same conditions, but using red light with a wavelength of 650 nm
instead, what effect will this have on the magnification and on the resolution of the microscope?
magnification resolution
A decreased decreased
B increased increased
C remains the same decreased
D remains the same increased
20. Which steps are needed to find the actual width of a xylem vessel viewed in transverse section using a
×40 objective lens?
1 Convert from mm to µm by multiplying by 10–3.
2 Calibrate the eyepiece graticule using a stage micrometer on ×10 objective lens.
3 Measure the width of the xylem vessel using an eyepiece graticule.
4 Multiply the number of eyepiece graticule units by the calibration of the eyepiece graticule.
A 1, 2, 3 and 4
B 2, 3 and 4 only
C 1 and 2 only
D 3 and 4 only
THE END
Questionnaire prepared by: Md. Emrul Hasan, Teacher (Biology), SFX Greenherald Int’l School
1. When using a compound microscope, objective lenses can be found to have a magnification of all of
the following, EXCEPT?
a. 4X b. 10X c. 40X d. 100X e. 1000X
2. What is "compound microscope"?
a. Microscope with the capability to view oil immersion
b. Microscope with the capability to view compounds
c. Microscope with a single lens
d. Microscope with two lenses
e. Microscope with three lenses
3. What is the total magnification achieved with a compound microscope?
a. Magnification of objective lens
b. Magnification of ocular lens
c. Magnification of ocular lens added to the magnification of the objective lens
d. Magnification of ocular lens multiplied by the magnification of the objective lens
e. Magnification of condenser lens multiplied by the magnification of the objective lens
4. What is the maximum resolving power seen with a compound microscope?
a. 2 millimeters b. 0.2 millimeters
c. 2 micrometers d. 0.2 micrometers
e. 2 angstroms
5. What is the turret?
a. Base b. Nosepiece c. Stage d. Tube e. Diaphragm
6. On a microscope, what structure connects the eyepiece to the objective lens?
a. Base b. Nosepiece c. Stage d. Tube e. Diaphragm
7. In a good compound microscope, the focus knob does not have to be readjusted when changing the magnification. What is this phenomenon called?
a. Parfocal b. Unifocal c. Bifocal d. Focused e. Convergent
8. What is another name for the light microscope?
a. Simple microscope b. Compound microscope
c. Phase contrast microscope d. Dissection microscope
e. Transmission electron microscope
9. Which microscope does not rely on visible light?
a. Simple microscope b. Compound microscope
c. Phase contrast microscope d. Dissection microscope
e. Transmission electron microscope
10. Which microscope makes things appear three dimensional?
a. Simple microscope b. Compound microscope
c. Phase contrast microscope d. Dissection microscope
e. Transmission electron microscope
11. When using a compound microscope, what is the magnification of the oil immersion lens?
a. 4X b. 10X c. 40X d. 100X e. 1000X
12. What is the usual magnification of the ocular lens on a compound microscope?
a. 1X b. 10X c. 100X d. 1000X e. 10,000 X
13. When using oil immersion to view a tissue, what is the refractive index of the oil?
a. Zero b. as air c. as glass d. as water e. None
14. What is the role of the condenser lens?
a. Control the aperture of light b. Increase the magnification
c. Focus the light on the specimen d. Initial magnification of 10X
e. Provide light
15. On a microscope, what structure varies the diameter of the cone of light?
a. Base b. Nosepiece c. Stage d. Tube e. Diaphragm
16. Where do you place the slide when using a microscope?
a. Base b. Nosepiece c. Stage d. Tube e. Diaphragm
17. What is the bottom of a microscope called?
a. Base b. Nosepiece c. Stage d. Tube e. Diaphragm
18. What is another name for the bright field microscope?
a. Simple microscope b. Compound microscope
c. Phase contrast microscope d. Dissection microscope
e. Transmission electron microscope
19. Which microscope would be particularly useful for looking at living cells?
a. Simple microscope b. Compound microscope
c. Phase contrast microscope d. Dissection microscope
e. Transmission electron microscope
20. Which type of microscope has only one lens?
a. Simple microscope b. Compound microscope
c. Phase contrast microscope d. Dissection microscope
e. Transmission electron microscope
THE END
Questionnaire prepared by: Md. Emrul Hasan, Teacher (Biology), SFX Greenherald Int’l School
Answer Key to Questions of Microscopy
1. e
2. d
3. d
4. d
5. b
6. d
7. a
8. b
9. e
10. d
11. d
12. b
13. c
14. c
15. e
16. c
17. a
18. b
19. c
20. a
1. When using a compound microscope, objective lenses can be found to have a magnification of all of the following, EXCEPT?
a. 4X
b. 10X
c. 40X
d. 100X
e. 1000X
Answer: e
A compound microscope has two lenses: an eyepiece lens and objective lens. The eyepiece lens usually has a magnification of 10X. There are objective lenses on the revolving nosepiece with varying magnifications. Most compound microscopes have objective lenses with magnification of 4X, 10X, and 40X. Some compound microscopes also have an oil immersion lens with a magnification of 100X.
2.
What is "compound microscope"?
a. Microscope with the capability to view oil immersion
b. Microscope with the capability to view compounds
c. Microscope with a single lens
d. Microscope with two lenses
e. Microscope with three lenses
Answer: d
A compound microscope has two lenses: an eyepiece lens and objective lens. The eyepiece lens usually has a magnification of 10X. There are objective lenses on the revolving nosepiece with varying magnifications. Most compound microscopes have objective lenses with magnification of 4X, 10X, and 40X. Some compound microscopes also have an oil immersion lens with a magnification of 100X.
3.
What is the total magnification achieved with a compound microscope?
a. Magnification of objective lens
b. Magnification of ocular lens
c. Magnification of ocular lens added to the magnification of the objective lens
d. Magnification of ocular lens multiplied by the magnification of the objective
lens
e. Magnification of condenser lens multiplied by the magnification of the
objective lens
Answer: d
To calculate the total magnification achieved with a compound microscope, the magnification of the ocular lens is multiplied by the magnification of the objective lens. For example, if viewing a sample with the 40x objective, the total magnification would be calculated as follows: a 10X ocular lens used with a 40X objective lens, the total magnification is 400X (10 x 40).
4. What is the maximum resolving power seen with a compound microscope?
a. 2 millimeters
b. 0.2 millimeters
c. 2 micrometers
d. 0.2 micrometers
e. 2 angstroms
Answer: d
Resolving power is the ability to see two things as discrete images. With normal vision, there is a resolving power of about of 100 micrometers. A compound microscope has a resolving power of approximately 0.2 micrometers. In other words, two marks 0.2 micrometers apart can be seen as two distinct entities. Any closer than this, they are perceived as one object.
5. What is the turret?
a. Base
b. Nosepiece
c. Stage
d. Tube
e. Diaphragm
Answer: b
The base is the bottom of the microscope. The revolving nosepiece is also called a turret. The objective lens are attached to the nosepiece (or turret). The slide rests on the stage. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube; thus, its name). The diaphragm controls the diameter of the cone of light.
6. On a microscope, what structure connects the eyepiece to the objective lens?
a. Base
b. Nosepiece
c. Stage
d. Tube
e. Diaphragm
Answer: d
The base is the bottom of the microscope. The revolving nosepiece is also called a turret. The objective lens are attached to the nosepiece (or turret). The slide rests on the stage. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube; thus, its name). The diaphragm controls the diameter of the cone of light.
7. In a good compound microscope, the focus knob does not have to be readjusted when changing the magnification. What is this phenomenon called?
a. Parfocal
b. Unifocal
c. Bifocal
d. Focused
e. Convergent
Answer: a
Parafocal is the term used for a microscope if the focus knob does not have to be readjusted when changing the magnifications. This phenomenon is seen with good compound microscopes. In other words, when the specimen is in focus at 4X and the objective is switched to 10X, the specimen remains in focus.
8. What is another name for the light microscope?
a. Simple microscope
b. Compound microscope
c. Phase contrast microscope
d. Dissection microscope
e. Transmission electron microscope
Answer: b
A simple microscope has only one lens. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". A phase contrast microscope is useful for examining living cells, because the specimen does not need to be stained. A dissection microscope uses low power magnification. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light, but rather a beam of electrons.
9. Which microscope does not rely on visible light?
a. Simple microscope
b. Compound microscope
c. Phase contrast microscope
d. Dissection microscope
e. Transmission electron microscope
Answer: e
A simple microscope has only one lens. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". A phase contrast microscope is useful for examining living cells, because the specimen does not need to be stained. A dissection microscope uses low power magnification. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light, but rather a beam of electrons.
10. Which microscope makes things appear three dimensional?
a. Simple microscope
b. Compound microscope
c. Phase contrast microscope
d. Dissection microscope
e. Transmission electron microscope
Answer: d
A simple microscope has only one lens. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". A phase contrast microscope is useful for examining living cells, because the specimen does not need to be stained. A dissection microscope uses low power magnification. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light, but rather a beam of electrons.
11. When using a compound microscope, what is the magnification of the oil immersion lens?
a. 4X
b. 10X
c. 40X
d. 100X
e. 1000X
Answer: d
A compound microscope has two lenses: an eyepiece lens and objective lens. The eyepiece lens usually has a magnification of 10X. There are objective lenses on the revolving nosepiece with varying magnifications. Most compound microscopes have objective lenses with magnification of 4X, 10X, and 40X. Some compound microscopes also have an oil immersion lens with a magnification of 100X.
12. What is the usual magnification of the ocular lens on a compound microscope?
a. 1X
b. 10X
c. 100X
d. 1000X
e. 10,000 X
Answer: b
The usual magnification of an ocular lens on a compound microscope is 10X. Some microscopes have a 15X eyepiece lens. The ocular lens is the lens at the top of the tube, the one that you first look through when using a microscope. It is also called the eyepiece lens.
13. When using oil immersion to view a tissue, what is the refractive index of the oil?
a. Zero
b. Same as air
c. Same as glass
d. Same as water
e. None of the above
Answer: c
With light microscopy, there normally is a space of air between the slide and the lens. Oil immersion replaces that space of air with oil. The refractive index of the oil is the same as glass.
14. What is the role of the condenser lens?
a. Control the aperture of light
b. Increase the magnification
c. Focus the light on the specimen
d. Initial magnification of 10X
e. Provide light
Answer: c
The role of the condenser lens is to focus light on the specimen. It is used with higher magnifications.
15. On a microscope, what structure varies the diameter of the cone of light?
a. Base
b. Nosepiece
c. Stage
d. Tube
e. Diaphragm
Answer: e
The base is the bottom of the microscope. The revolving nosepiece is also called a turret. The objective lens are attached to the nosepiece (or turret). The slide rests on the stage. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube; thus, its name). The diaphragm controls the diameter of the cone of light.
16. Where do you place the slide when using a microscope?
a. Base
b. Nosepiece
c. Stage
d. Tube
e. Diaphragm
Answer: c
The base is the bottom of the microscope. The revolving nosepiece is also called a turret. The objective lens are attached to the nosepiece (or turret). The slide rests on the stage. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube; thus, its name). The diaphragm controls the diameter of the cone of light.
17. What is the bottom of a microscope called?
a. Base
b. Nosepiece
c. Stage
d. Tube
e. Diaphragm
Answer: a
The base is the bottom of the microscope. The revolving nosepiece is also called a turret. The objective lens are attached to the nosepiece (or turret). The slide rests on the stage. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube; thus, its name). The diaphragm controls the diameter of the cone of light.
18. What is another name for the bright field microscope?
a. Simple microscope
b. Compound microscope
c. Phase contrast microscope
d. Dissection microscope
e. Transmission electron microscope
Answer: b
A simple microscope has only one lens. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". A phase contrast microscope is useful for examining living cells, because the specimen does not need to be stained. A dissection microscope uses low power magnification. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light, but rather a beam of electrons.
19. Which microscope would be particularly useful for looking at living cells?
a. Simple microscope
b. Compound microscope
c. Phase contrast microscope
d. Dissection microscope
e. Transmission electron microscope
Answer: c
A simple microscope has only one lens. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". A phase contrast microscope is useful for examining living cells, because the specimen does not need to be stained. A dissection microscope uses low power magnification. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light, but rather a beam of electrons.
20. Which type of microscope has only one lens?
a. Simple microscope
b. Compound microscope
c. Phase contrast microscope
d. Dissection microscope
e. Transmission electron microscope
Answer: a
A simple microscope has only one lens. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". A phase contrast microscope is useful for examining living cells, because the specimen does not need to be stained. A dissection microscope uses low power magnification. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light, but rather a beam of electrons.
