MICROSCOPES Copyright Cmassengale 1. Microscopy and Measurement produce an enlarged image of an...
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Transcript of MICROSCOPES Copyright Cmassengale 1. Microscopy and Measurement produce an enlarged image of an...
MICROSCOPES
Copyright Cmassengale 1
Microscopy and Measurement Microscopes – produce an enlarged
image of an object Used to study organisms, cells, and cell parts Increase in apparent size is called magnification The ability to show details clearly is called
resolution Microscopes vary in both magnification and
resolution
Copyright Cmassengale 2
Compound Light Microscopes
Specimen mounted on a glass slide
Must be thinly sliced or very small
Pair of lenses Ocular lens (eye
piece) Objective lens (nose
piece) Can be used to
study LIVE specimensCopyright Cmassengale 3
EYEPIECE
BODY TUBE
ARM
STAGE
COURSE ADJ.
FINE ADJUSTMENT
BASE
NOSEPIECE
OBJECTIVE
STAGE CLIPS
DIAPHRAGM
LIGHT SOURCE
OBJECTIVE
OBJECTIVE
Take a quiz!
Magnification
Magnification determined by multiplying power of both lenses
Eyepiece 10X times Objective power (20X, 40X…)
Highest Maximum magnification is around 1000X
Copyright Cmassengale 6
Electron Microscope Transmission EM (TEM)
Uses a beam of electrons to produce an enlarged image of very thinly sliced specimen on screen or photographic plate
Image focused by magnetic lenses
200,000X magnification Cannot be used to view
living specimens
Copyright Cmassengale 7
Scanning EM (SEM) 3D image Specimens not sliced for
viewing Surface sprayed with fine
metal coating Also uses electron beam
and fluorescent screen or photographic plates
100,000X magnification Cannot be used to view
living specimens
Copyright Cmassengale 8
Electron Microscope:
Click for Video on how an Electron Microscope works
We are notWe are not alone.flv alone!
Microscope Care and Use
Always carry the microscope by holding the arm of the microscope with one hand and supporting the base with the other hand
Place the microscope on a flat surface. The arm should be positioned toward you
Look through the eyepieces. Adjust the diaphragm so that light comes through the opening in the stage
• Place a slide on the stage so that the specimen is in the field of view. Hold it firmly in place by using the stage clips• Always focus first with the coarse adjustment and the low-power lens. Once the object is in focus on low power, the high-power objective can be used. Use ONLY the fine adjustment to focus the high-power lens.• Store the microscope covered.
