02 Microscope

ABCarrying a Microscope

Return the lowest power objective in placeWrap the cord around the baseReturn dustcoverStoring The Microscope

Use lens paper on all glass parts of the microscope.Clean oil immersion lens with chemicals provided by your instructorCleaning the Microscope

Parts of the MicroscopeFine adjustment knobStageStage clipsApertureDiaphragmLightBaseOcularBody tubeNosepieceObjective lenses (10, 40, 100x)ArmCourse adjustment knob

Microscope PartsOcularBody tubeHeadRevolving nose pieceObjectiveArmStageDiaphragmLever to move stage clipCourse adjustmentFine adjustmentLight sourceBase

Using the microscopeAlways observe using the LOWEST POWER objective first. Focus using the COARSE ADJUSTMENT KNOB to bring the object into focus. Bring the object into sharp focus by using the fine adjustment knob. Focus, and then move to a higher power objective, if needed. Use only the FINE ADJUSTMENT KNOB when using the HIGHEST (longest) POWER OBJECTIVE. Keep both eyes open to reduce eyestrain. Determine total magnification of the object by multiplying the power of the ocular (10x) the power by the power of the objective.

Preparing a slideUsing a pipet or dropper, add a drop of water or another solvent to a clean microscope slide. Then, place the specimen in the water. Place the edge of a coverslip on the slide so that it touches the edge of the water. Slowly lower the coverslip to prevent the formation of air bubbles.

40x100xOil Immersion Lens

Animal Cell

Plant Cell

Cheek Cell

Elodea

Epidermal Onion Cell

Mitosis

MitosisInterphaseProphaseLate Prophase

MitosisMetaphaseAnaphaseTelophase

Mitosis

Dispose Biological material

Dispose Sharps

Clean Up

Microscope Parts Quiz

Microscope QuizWhich objective uses oil?If your ocular is 10x and your objective is 40x what is the total magnification of your image?Why do you need a cover slip and how do you avoid an air bubble?Where do you dispose slides?

P. 45 lab manualspermbloodsmooth muscleSimple squamous epithelial

Cheek Cell0.9% saline

Cheek CellsalineScrape inside cheekSwirl toothpick with cells in salinePlace a small drop methylene blue on saline

Cheek CellPlace coverslipObserve under microscopeDraw what you seeClean up

Cheek Cell

Pond waterYeast