Microbiology Unknown Lab BIO 732. TSI agar Triple Sugar Iron Agar (a) Red/red (b) control (c)...
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Transcript of Microbiology Unknown Lab BIO 732. TSI agar Triple Sugar Iron Agar (a) Red/red (b) control (c)...
Microbiology Unknown LabBIO 732
TSI agarTriple Sugar Iron Agar
(a) Red/red
(b) control
(c) Red/yellow
(d) Yellow/yellow
(e) Red/yellow with H2S
A B C D E
Penol Red broths
A B C D E
A (acid with gas)…..B (acid)……C (uninoculated control)……D (no recation)… …E (alkaline reaction)
O-F tests
Control E. coli Pseudomonas Alcaligenes
Fermenter Oxidizer Nonsaccharolytic
Motility Test
Decarboxylation
Positive Negativeuninoculated
Gelatin Hydrolysis
Simmons Citrate
A B C
A: Positive…Enterobacter
B: Negative…E. coli
C: Control-uninoculated
Phenylalanine Deaminase Test
neg pos
Urea agar
pos uninoculated neg
Methyl Red Test
A B
A: Positive (E. coli) B: Negative (Enterobacter aerogenes)
Voges Proskauer Test
A B
A: Negative (E. coli) B: Positive (Enterobacter aerogenes)
Indole TestNegative Positive
- +
Catalase Test
+ -
Positive Negative
GRAM STAIN