Microbiology Unknown LabBIO 187

TSI agarTriple Sugar Iron Agar

(a) Red/red (no sugar fermentation)

(b) Control

(c) Red/yellow (Glucose fermented but lactose and sucrose not fermented)

(d) Yellow/yellow (Glucose fermented. Lactose and/or sucrose fermented)

(e) Red/yellow with H2S

A B C D E

0.1% dextrose

1.0% sucrose

1.0% lactose

Penol Red broths

A B C D E

A (acid with gas)…..B (acid)……C (uninoculated control)……D (no recation)… …E (alkaline reaction)

O-F tests

Control E. coli Pseudomonas Alcaligenes

Fermenter Oxidizer Nonsaccharolytic

Motility Test

Decarboxylation

Positive Negativeuninoculated

Gelatin Hydrolysis

Simmons Citrate

A B C

A: Positive…Enterobacter

B: Negative…E. coli

C: Control-uninoculated

Phenylalanine Deaminase Test

neg pos

Urea Hydrolysis

Positive uninoculated

Negative

Methyl Red Test

A B

A: Positive (E. coli) B: Negative (Enterobacter aerogenes)

Voges Proskauer Test

A B

A: Negative (E. coli) B: Positive (Enterobacter aerogenes)

Indole TestNegative Positive

- +

Catalase Test

+ -

Positive Negative

GRAM STAIN

General Microbiology (BIOL 187) Laboratory Report (Unknown Identification) 

Page 1: Cover page

Include your name, the unknown number and you final Genus and species identification

 

Page 2: Test listing

I want a (+) / (-) listing of each test in a vertical, easily scanned format

Page 3: Discussion section

Detail, in complete sentences, why your results justify your identification and rule out other possibilities.

 

Page 4: About My Microbe section

Research your microbe and give me a several paragraphs about your microbe.

(Don't copy word-for-word from your textbook---This will be frowned upon!)

 

Page 5: Dichotomous key

Create a neat dichotomous key, which results in the correct separation of all 8 possible unknowns. This key should be legible and sized to occupy a complete sheet of paper (nothing microscopic is acceptable here)

 

Page 6:Lab handout where you recorded your initial results

Include the handout from lab where your recorded your data as you read the test reactions.

 

Grading:

20 pts. Correct Identification

10 pts. Discussion section

7 pts. "About my microbe section"

8 pts. Dichotomous key

5 pts. Neatness, accuracy and completeness of report

50 points total