MICROBIOLOGY 20 - California State University, Fresnozimmer.csufresno.edu/~sdonohue/Micro 20.pdf ·...
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" MICROBIOLOGY 20 Introduction to Microbiology California State University Fresno, Fall 2004 4 units lecture: MWF 9:00-9:50am, McL 162 Lab: two labs/week as assigned, S-222 Website: http://blackboard.csufresno.edu Instructor: Dr. Judy Haber Email: [email protected] Telephone: 278-2362 Office: S-224A Office Hrs: T,Th 1:30-2:00pm, F10-12 OBJECTIVES: . To learn the morphology, physiology, classification, identification, and pathogenicity of microorganisms and their role in the ecosystem and in disease. -To develop an understanding of the diversity of microbial morphology and physiology and integrate that understanding into the classification and identification of microorganisms. -To learn how microorganisms cause disease, how the immune system fights disease, how to grow microorganisms in order to identify them, and how to control their growth to prevent and treat disease. -To be aware of the relevance of Microbiology in the real world and in our daily lives. -To master laboratory procedures necessary to work with-and identify microorganisms, especially aseptic techniques and microscopy. P~EREQIJI~ITE~' Biology, Chemistry and Organic Chemistry highly recommended Familiarity with basic chemical and biological principles TEXTS: A. MICROBIOLOGY AN INTRODUCTION, Tortora, Funke, and Case, 8th edition B. PHOTOGRAPHIC ATLAS packet (available with text or separately) C. Microbiology 20 LABORATORY EXPERIMENTS -download from Blackboard D. All lecture outlines and class material will be posted on Blackboard. MATERIALS: A. B. C. D. E. F. Lab coat required, loaners available 1 boxplainglass microbiology slides plus coverslips Sharpiepermanentmarkerand Chinamarkingpencil Colored pencils Clear plastic mm ruler Scantronsand pencilwithgood eraser ATTENDANCE POLICY: A. Students are expected to attend all class sessions, lectureand lab, and to arrive on time. Absence without communication during the first 2 weeks of lab willresult in your seat being given to another student. 3 tardies equal one absence. leaving lab early without permission equals an absence. 25 points willbe allotted for participation,-including unannounced written exercises in lecture, eGuide quizzes, and attendance. participation, written lab work, and cleanup in lab. Ifabsent from class, writtenparticipation assignments may not be made up. Unexcused lab absences will- result in a 5 pointdeduction. B. SPECIAL NEEDS If you have any special needs as addressed by the Americans with Disabilities Act, notify "M.., ,..,.',. the instructor. Eve!YE~C)"~2~apl~_~Jfo~~illl:>l:}l1'1C)(je tel-a~rnm()(jat~ ~~LI1~~s. -.. ""n ' n_'____----..
Transcript of MICROBIOLOGY 20 - California State University, Fresnozimmer.csufresno.edu/~sdonohue/Micro 20.pdf ·...
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MICROBIOLOGY 20Introduction to Microbiology
California State University Fresno, Fall 20044 units
lecture: MWF 9:00-9:50am, McL 162
Lab: two labs/week as assigned, S-222
Website: http://blackboard.csufresno.edu
Instructor: Dr. Judy Haber
Email: [email protected]
Telephone: 278-2362Office: S-224A
Office Hrs: T,Th 1:30-2:00pm, F10-12
OBJECTIVES: .
To learn the morphology, physiology, classification, identification, and pathogenicity ofmicroorganisms and their role in the ecosystem and in disease.
-To develop an understanding of the diversity of microbial morphology and physiology andintegrate that understanding into the classification and identification of microorganisms.
-To learn how microorganisms cause disease, how the immune system fights disease, how togrow microorganisms in order to identify them, and how to control their growth to preventand treat disease.
-To be aware of the relevance of Microbiology in the real world and in our daily lives.-To master laboratory procedures necessary to work with-and identify microorganisms,especially aseptic techniques and microscopy.
P~EREQIJI~ITE~'Biology, Chemistry and Organic Chemistry highly recommendedFamiliarity with basic chemical and biological principles
TEXTS:A. MICROBIOLOGY AN INTRODUCTION, Tortora, Funke, and Case,8th editionB. PHOTOGRAPHIC ATLAS packet (available with text or separately)C. Microbiology 20 LABORATORY EXPERIMENTS -download from BlackboardD. All lecture outlines and class material will be posted on Blackboard.
MATERIALS:A.B.C.D.E.F.
Lab coat required, loaners available1 boxplainglass microbiologyslidespluscoverslipsSharpiepermanentmarkerand ChinamarkingpencilColored pencilsClear plastic mm rulerScantronsand pencilwithgooderaser
ATTENDANCE POLICY:A. Students are expected to attend all class sessions, lectureand lab, and to arrive
on time. Absence without communication during the first 2 weeks of lab willresultin your seat being given to another student. 3 tardies equal one absence.leaving lab early without permission equals an absence.25 points willbe allotted for participation,-including unannounced writtenexercises in lecture, eGuide quizzes, and attendance. participation, written labwork, and cleanup in lab. Ifabsent from class, writtenparticipation assignmentsmay not be made up. Unexcused lab absences will-result in a 5 pointdeduction.
B.
SPECIAL NEEDSIfyou have any special needs as addressed by the Americans with Disabilities Act, notify
"M.., ,..,.',. the instructor. Eve!YE~C)"~2~apl~_~Jfo~~illl:>l:}l1'1C)(jetel-a~rnm()(jat~ ~~LI1~~s.-.. ""n ' n_'____----..
GRADING:
A Grades willbe based on a percenta9~ of the total points:
85-100%75-84%65-74%55-64%0 -54%
ABC0F
B. The points willbe distributed as follows(tentative, subject t6Change):
4 lecture exams @ 100 points each1,comprehensive final lecture exam3 laboratory exams@ 50 points eachBacterial unknowns,News article (required to pass, Due by Nov23)Participation (including in class writingassigns)Total points
4002001505015
at least 25840
C. Allgrades willbe available on Blackboard.
D.Exams willconsist of multiplechoice,true and false, matching, and short essay.Students willneed scantrons and a good eraser.
E. Ifyou are having difficultieswiththe material, please see your instructor for hetp~$soon as possible. Free tutoring is available through the Learning Resource Center. Inorder to drop after the drop deadline the dean may require that the student providewritten substantiation as deemed necessary.
MAKEU_PS_AND LATE WORK
Make-ups willbe given onlyfor serious and compelling reasons with documentation required.Any late work willbe subject to a 20% grade penalty per day late. No unknowns ~ccepted late.
ACADEMIC DISHONESIY
University policyon cheating and plagiarism willbe strictlyenforced. See UniversityCatalog.
LABORATORY SAFETY:RULES
A. Allwork areas must be kept as clean as possible. Keep all unnecessary books,papers, etc. off the wor\<.area.Disinfect the work area at the beginning and the end of each lab period.,Aseptic techniques must be used at all times. ~.Lab coats are required to be worn during lab and should be kept in the lab at all times.Long hair must be tied back. '
Allmaterials must be handled withcare. They may be dangerous, or at the veryleast, cause permanent stains.Any spills of livingcultures musU)e reported to-the instructor at once.
NOTHINGis to be placed .in:yourmouth while in the laboratory. This includespipettes, food, gum, drinks, pencils or fingernails. No food or drink anowed in the lab.Wash hands thorougl1lybefore leaving lab, especially before eating.Closed shoes should be worn during labs. Rubber-soled shoes arerecor;n~ended.Organisms used in class may be pathogenic ,and Shouldbe treated withdue respect.'Careless or hazardous behavior willresu,1tin decr.Qasedgrade or dismissal.
B.C.D.
E.
F.G.
H.I.J.
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LECTURE TOPICS (Tentative schedule, subject to change):
DATE
8/238/258/309/19/3,8,109/69/139/159/1791209/249/22,27,2910/1,310/610/810/11
. 10/13,1810/1510/18-2010/2210/2510/2710/2911/111/311/5,811/1011/12,1511/1711/1911/2211/24-2611/2912/1
12/312/612/8
.Finalexam
TOPIC CHAPTER
Introduction,OverviewHistorical developments in MicrobiologyMicroscopyChemical PrinciplesMicrobial StructureLabor Day HoiidayMicrobial growthComplete, ReviewLecture exam 1Control of microbial growth, physicalControl of miqr()bialgrowth, chemicalMicrobial metabolismMicrobial geneticsComplete, ReviewLecture exam 2Classification of microorganismsBacteria and bacterial diseasesMicrobial EvolutionFungi, Protozoa and HelminthsVIrusesPrinciples of disease and epidemiologyComplete, ReviewLecture Exam 3
Mechanisms of pathogenicityNonspecific defense mechanisms of the hostSpecific defense mechanisms: ImmunologyImmune system disordersAntimicrobial drugsComplete, ReviewMicrobial EcologyLecture Exam 4Thanksgiving VacationSpecimen CollectionDiseases
11324
6
1, 2,3,4,67758
5,7,81011
121314
10-131516171920
14-17,(19),20
21-26+disease handout
2728
Environmental & Water Microbiology,AppliedMicrobiology .
Review
Monday Dee 13 8A5 -10:45am Comprehensive
This syllabus and schedule are subject to change. If you are absent from class, it isyourresponsibility to check on announcements made whileyou were absent. Also checkannouncements section on Blackboard regularly.
For any questions pertaining to this class, email instructor via Blackboard link using yourCSUFresno email address. In subject line, put Micro 20. Sign your full name at the end of
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Lab Schedule (subject to change)
Date Lab Exercise
12/6-7
Check-in, lab rules, Safety, eGuideMicroscopy,transferSmear preparation, simple stain, wet mountGram stain, negative stain, microscopic measurementHOLIDAY ,
Acid-fast stainEndospore stain, Capsule stainCapsule stain, st~in reviewPure culturetechniques,stainrevi~w ,
Lab Exam 1 Unit 1 Microscbpy(~nd' StainingBacterial population countsTemperature effects, lethal effects qJheat ,
Osmotic pressure, oxygen requirements, Cultivationof anaerobespH effects, disinfectants and antiseptics, ~kin.normalflora and disinfectionAntibioticsusceptibility testing, effectiveness of hand washingMedia preparationUltravioletlight effects ,
Wateranalysis-presumptivetestReview "
Water analysis- confirmatorytest "
Lab Exam 2 Unit 2 Growth and'Control'ofGrowthWater analysis- completed testBuildunknownflowchartWater analysis- read 9OffipletedJE3stStart unknowns: Stock CUlturepreparation, Microscppic morphology-stainsCultural characteristics of microorganisms, s~lective media, catalase, oxidaseSugar fermentation, Starch hydrolysis, gelatinase, thioglyconateIMViCtests: indole, MRVP,citrateNitrate, urea, motility,TSIDecarboxylase testIsolationand identificationof Staphylococcus,' Streptococcus, EnterococcusStaphylocoCCUs,Streptococcus, EnterococcusComplete unknownsThanksgiving VacationThroat culture, Complete unknowns, Case studiesRead throat cultures, Clean-up and check-out, ReviewAll Unknowns DueLab Exam 3 Unit 3 Water Testing and Bacterial Identification Tests
8/23-248/25-268130-319/1-2916-79/8-99/13-149/15-169/20-219122-239/27-289129-3010/4-510/6-710/11-1210/13-1410/18-19
10/20-21
10/25-26
10127-28
11/1-21113-41118-911/10-1111/15-16
11/17-1811/22-2311124-2511/29-3012/1-2
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