Microbiological stabilization of Beer-Mix beverages with ... · Microbiological stabilization of...

LANXESS Deutschland GmbH, Paul Marquardt

Vienna, 08.09.2014

Microbiological stabilization of Beer-Mix beverages

with Dimethyl-Dicarbonate – first practical experiences

1 Business Unit Material Protection Products

DMDC – Genie in a bottle

Product Launches

DMDC Technology

Microbiology & Sensory

Registration

Application

2 Title of presentation ▪ Speaker ▪ Place, date … [ OPTIONAL ]

Beer-Mix beverages is a very innovative product category

60

84

15

4 4 Europe

NorthAmerica

Middle East& Africa

Asia Pacific

Product introduction by Regions 2013* Global product launches by flavour, 2009-13*

* Mintel , Beer Mixes, Feb 2014

29%

24% 6% 4%

4% 3%

30%

Lemon

Lemonade

Grapefruit

Cola

Apple

Lime

Others Product launches by packing*

60% 40%



Product Launches

DMDC Technology


Registration

Application


DMDC has a unique operating principle

lipophilic

cell membrane

[pH : 2 - 4 (acidic)] [pH : 7 (neutral)]

inactivated enzyme with

carbo-methoxylated imidazole group

penetration

effect

on

250 mg dimethyl-dicarbonate

water

120 mg

methanol

natural occurring

substances 162 mg

carbon-dioxide

+

H3C O O O

O

CH3

O

O 2

+ H3C

OH 2 CO2 2

H

active enzyme with

Imidazole group

Beer Mix Beverage Cell interior

N

N

R2

H

R1

H


DMDC hydrolyses rapidly, especially in warmer environments

0 %

20 %

40 %

60 %

80 %

100 %

0' 30' 60' 90' 120' 150' 180' 210' 240'

Time (Minutes)

DM

DC

20 °C / 68°F

10 °C / 50°F

4 °C / 39°F

Hydrolysis: DMDC + Water = Methanol + CO2



Product Launches

DMDC Technology


Registration

Application


DMDC needs to be dosed inline to the finished Beer-Mix beverage with specialized dosing equipment


De- centralization of production to sites without tunnel-pasteurizers

Flexible use for heat-sensitive packaging

Lower energy consumption

Reduction of foot print

Replacement of tunnel-pasteurizer

Improve taste

DMDC targets relevant micro- spectrum yeasts

Clean label in many countries

Remove Persistent preservatives

Bottling more sensitive beer-mix beverages

Switch from sugar-free to “full” version beer mix beverages

Highly flexible application

Low investment

Utilize existing beer filling machines

Beer-Mix beverages – opportunities with DMDC

http://www.stuffintheair.com/metric-conversion.html


Beer-Mix beverages – first experiences with DMDC

First Customer Projects:

Discussion about Beer-Mix beverages in heat-sensitive packing for export

Trials in Wheat Beer-Mix beverage from sugar-free to “full” version

Trials with “Radler” on existing bottling lines

Running business:

Customers in Germany with Velcorin® in Beer-Mix beverages on existing

bottling lines in order to secure microbiological quality.

Velcorin® for Beer-Mix beverages



Product Launches

DMDC Technology


Registration

Application


Internal microbiological challenge test – microorganisms

Beer Mix

Lager Beer + Lemon soda

pH = 3,4

Microorganisms

Pichia anomala

Candida tropicalis

Saccharomyces cerevisiae

Zygosaccharomyces bailii Bier Mix

Wheat Beer + Lemon

soda

pH = 3,4

Matrix

Lactobacillus brevis


Internal microbiological challenge test-parameters

Parameter

Beer Mix

Lager Beer + Lemon soda

pH = 3,4

Temperature

Germ loading

DMDC concentration

Value

5-10 °C

Target: 30 CFU/ml and 300 CFU/ml

0 ppm and 250 ppm

Replication Duplicates

Incubation 18 – 20 °C for 7 days

Bier Mix

Wheat Beer + Lemon

soda

pH = 3,4

Matrix

Nutrient Media MRS Agar & Malt Extract Agar


Internal microbiological challenge test-results

Matrix

Germ Loading

Start 1 Week Start 1 Week Start 1 Week Start 1 Week

Matrix Amount 1 mL 10 mL 1 mL 10 mL 1 mL 10 mL 1 mL 10 mL

54/49 20/14 640/540 +/+ 20/28 3/- 568/444 +/+

31/38 12/10 804/640 +/+ 26/25 -/- 616/520 +/+

48/41 -/- 536/616 -/- 22/20 -/- 604/570 -/-

32/45 -/- 632/740 -/1 19/31 -/- 536/636 -/-

Beer Mix - Lager Beer Mix - Wheat

0 ppm DMDC

250 ppm DMDC

I 30 CFU/ml II 300 CFU/ml I 30 CFU/ml II 300 CFU/ml

Pichia anomala

Candida tropicalis

Matrix

Germ Loading



1/1 -/- 84/76 4/6 -/- -/- 152/125 1/3

-/3 -/- 123/108 -/- -/- -/- 138/126 77/45

2/1 -/- 68/71 -/- -/- -/- 133/148 -/-

1/3 -/- 126/93 -/- -/- -/- 127/165 -/1

II 300 CFU/ml

0 ppm DMDC

250 ppm DMDC


I 30 CFU/ml II 300 CFU/ml I 30 CFU/ml


Saccharomyces cerevisiae

Zygosaccharomyces bailii

Matrix

Germ Loading



28/26 +/+ 324/252 +/+ 29/27 90/98 298/314 +/+

34/25 +/+ 312/308 +/+ 24/27 24/25 294/342 +/+

24/22 -/- 260/256 -/- 27/27 -/- 340/266 -/-

30/30 -/- 300/308 -/- 34/20 -/- 258/234 -/-

II 300 CFU/ml

0 ppm DMDC

250 ppm DMDC


I 30 CFU/ml II 300 CFU/ml I 30 CFU/ml

Matrix

Germ Loading



23/27 1/- 274/240 16/23 30/13 1/2 352/280 3/1

30/18 -/- 260/273 5/5 20/20 5/9 384/280 24/21

20/14 -/- 276/215 -/- 24/28 -/- 262/206 -/-

25/22 -/- 238/232 -/- 18/20 -/- 340/240 -/-


0 ppm DMDC

250 ppm DMDC

I 30 CFU/ml II 300 CFU/ml I 30 CFU/ml II 300 CFU/ml



No growth of Lactobacillus brevis with / without Velcorin® treatment. Microbe obviously

not adapted to the matrix

Lactobacillus brevis

Matrix

Germ Loading



4/1 -/- 85/67 -/- 2/2 -/- 94/77 -/-

-/- -/- 69/71 -/- 1/- -/- 77/74 -/-

2/- -/- 65/96 -/- 2/1 -/- 90/51 -/-

1/7 -/- 77/64 -/- 2/- -/- 84762 -/-

I 30 CFU/ml II 300 CFU/ml

0 ppm DMDC

250 ppm DMDC

I 30 CFU/ml II 300 CFU/ml




Sensory triangle test – no influence on taste

Lager type + lemon soda

Beer Mix

Lager type + lemon juice

Dealcoholized Lager type

+ lemon soda

Wheat beer + lemon soda

Lager type + energy drink

Triangle test according MEBAK II 2.34.7

No statistical relevant difference





Test performed by

Forschungszentrum

Weihenstephan August 2014:

Beverage treated with DMDC

against

beverage without DMDC


Further test at Forschungszentrum Weihenstephan für Brau- und Lebensmittelqualität

Lager type + lemon soda

Microorganisms to be tested

Lactobacillus brevis-FZ BLQ 4

Pectinatus frisingensis-FZ BLQ HB S1

Saccharomyces cerevisiae-FZ BLQ H TUM 68

Saccharomyces pastorianus ssp. Carlsbergensis

-FZ BLQ H TUM 34/70

Beer Mix

Saccharomyces cerevisiae var. Diastaticus

-FZ BLQ TUM SY 1

Lager type + lemon juice

Dealcoholized Lager type

+ lemon soda

Wheat beer + lemon soda

Lager type + energy drink

Dekkera anomala-FZ BLQ 2-C-1

Wickerhamomyces anomalus FZ BLQ 17-C-3

Kazachstania exigua-FZ BLQ H2-G-7

pH

Alcohol [Vol %]

BU

CO2 [GV-%]

F.S. [g/100ml]

Analytical parameters



Product Launches

DMDC Technology


Registration

Application


BMB with Lemon Soda and/ or Juice

BMB with Cola, energy drink, etc.

Alcohol free BMB

Sweetened / Unsweetened BMB

Flavoured Beer

Beer with Spirits

Malt Beer

Beer-Mix Beverages Categorization

Regulatory Categorisation of Beer Mix Beverages

Beverage Category Regulation (EC) No. 1333/2008, Annex II: Category No.: 14.2.8

Other alcoholic drinks including mixtures of alcoholic drinks with non-alcoholic drinks and spirits with less

than15% of alcohol

No registration of DMDC for Beer and Malt beverages (Category 14.2.1)

Labelling: Regulation (EC) No. 1169/ 2011, Article 16(4):

4. Without prejudice to other Union provisions requiring a list of ingredients or a mandatory nutrition declaration, the

particulars referred to in points (b) and (l) of Article 9(1) shall not be mandatory for beverages containing more than 1,2 % by

volume of alcohol. Therefore there is no obligation to label DMDC

Outside Focus Beer Beverages Focused Beer-Mix Beverages


Not present in final beverage

No declaration on ingredients list

No allergen or GMO

No effect on colour or taste

Compatible with all packaging

Extensive technical Service

Benefits and other applications of DMDC

Why Velcorin® ? Use of Velcorin in other Beverage categories

Carbonated & Still Juice Beverages Isotonic Sports Beverages

Ice Teas

Flavoured Waters Wines

Ciders


THANK YOU FOR YOUR ATTENTION


Disclaimer

-Presentation is no hand out, for presentation purposes and internal use only! -

This information and our technical advice - whether verbal, in writing or by way of trials - are given in good

faith but without warranty, and this also applies where proprietary rights of third parties are involved. Our

advice does not release you from the obligation to verify the information currently provided - especially that

contained in our safety data and technical information sheets - and to test our products as to their suitability

for the intended processes and uses. The application, use and processing of our products and the products

manufactured by you on the basis of our technical advice are beyond our control and, therefore, entirely

your own responsibility. Our products are sold and our advisory service is given in accordance with the

current version of our General Conditions of Sale and Delivery.

The handling of Velcorin® , as a chemical substance, is subject to the German Hazardous Substance

Ordinace and the corresponding EC Directive. The following hazard characteristics should be noted:

harmful (if swallowed), toxic (by inhalation), corrosive.


Due to Velcorin‘s properties, it can be regarded as a processing aid without requirement for labelling

Codex Alimentarius General Standard for Food Additives (Categories 14.1.4 and 14.1.5)

Inventory list of substances used as processing aids

EU (EC) No 1333/2008

USA Food Additive Provision and Food Contact Notification

Halal & Kosher

No Labelling Labelling

Germany

USA

Mexico

Russia

Australia/New Zealand

…

Turkey

Brazil

India

China

South Africa

…

References

Processing aid function Preservative function

Max. dosage in non-alcoholic soft-drinks: 250ppm


Velcorin also complies with ethnic and religous dietary laws. Certificates are available

Halal

Kosher

Issued by Halal Control Europe

Accredited & appointed by e.g.

-JAKIM (Department of Islamic Development Malaysia)

-LP-POM MUI Indonesia (Indonesian Council of Ulama)

Certificates available from

- Orthodox Union (OU)

- Kosher Supervision

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