Microbial Genetics (Ch 8) - Mt. SACfaculty.mtsac.edu/cbriggs/Microbial Genetics Ch 8 FA16.pdf ·...

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Microbial Genetics (Ch 8)

Transcript of Microbial Genetics (Ch 8) - Mt. SACfaculty.mtsac.edu/cbriggs/Microbial Genetics Ch 8 FA16.pdf ·...

Page 1: Microbial Genetics (Ch 8) - Mt. SACfaculty.mtsac.edu/cbriggs/Microbial Genetics Ch 8 FA16.pdf · Microbial Genetics (Ch 8) Author: Briggs, Christopher M. Created Date: 10/31/2016

Microbial Genetics (Ch 8)

Page 2: Microbial Genetics (Ch 8) - Mt. SACfaculty.mtsac.edu/cbriggs/Microbial Genetics Ch 8 FA16.pdf · Microbial Genetics (Ch 8) Author: Briggs, Christopher M. Created Date: 10/31/2016

Chromosome

Fig 8.1

Page 3: Microbial Genetics (Ch 8) - Mt. SACfaculty.mtsac.edu/cbriggs/Microbial Genetics Ch 8 FA16.pdf · Microbial Genetics (Ch 8) Author: Briggs, Christopher M. Created Date: 10/31/2016

Major genetic pathways

Fig 8.2

Page 4: Microbial Genetics (Ch 8) - Mt. SACfaculty.mtsac.edu/cbriggs/Microbial Genetics Ch 8 FA16.pdf · Microbial Genetics (Ch 8) Author: Briggs, Christopher M. Created Date: 10/31/2016

Bidirectional replication

Fig 8.6

Page 5: Microbial Genetics (Ch 8) - Mt. SACfaculty.mtsac.edu/cbriggs/Microbial Genetics Ch 8 FA16.pdf · Microbial Genetics (Ch 8) Author: Briggs, Christopher M. Created Date: 10/31/2016

Expression, intro

Fig 8.9

Page 6: Microbial Genetics (Ch 8) - Mt. SACfaculty.mtsac.edu/cbriggs/Microbial Genetics Ch 8 FA16.pdf · Microbial Genetics (Ch 8) Author: Briggs, Christopher M. Created Date: 10/31/2016

Simultaneous transcription + translation

Fig 8.10

Page 7: Microbial Genetics (Ch 8) - Mt. SACfaculty.mtsac.edu/cbriggs/Microbial Genetics Ch 8 FA16.pdf · Microbial Genetics (Ch 8) Author: Briggs, Christopher M. Created Date: 10/31/2016

Operons

Intro to lac operon: https://www.youtube.com/watch?v=oBwtxdI1zvk Operon lecture video: https://www.youtube.com/watch?v=10YWgqmAEsQ

Page 8: Microbial Genetics (Ch 8) - Mt. SACfaculty.mtsac.edu/cbriggs/Microbial Genetics Ch 8 FA16.pdf · Microbial Genetics (Ch 8) Author: Briggs, Christopher M. Created Date: 10/31/2016

Fig. 8.12

Operon model: Negative inducible

Page 9: Microbial Genetics (Ch 8) - Mt. SACfaculty.mtsac.edu/cbriggs/Microbial Genetics Ch 8 FA16.pdf · Microbial Genetics (Ch 8) Author: Briggs, Christopher M. Created Date: 10/31/2016

Fig. 8.13

Operon model: negative repressible

Page 10: Microbial Genetics (Ch 8) - Mt. SACfaculty.mtsac.edu/cbriggs/Microbial Genetics Ch 8 FA16.pdf · Microbial Genetics (Ch 8) Author: Briggs, Christopher M. Created Date: 10/31/2016

Fig. 8.14

Operon model

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Fig. 8.15

Operon model: Positive inducible, complex

Page 12: Microbial Genetics (Ch 8) - Mt. SACfaculty.mtsac.edu/cbriggs/Microbial Genetics Ch 8 FA16.pdf · Microbial Genetics (Ch 8) Author: Briggs, Christopher M. Created Date: 10/31/2016

Paper on other regulated genes: Toledo-Arana et al. 2009

Page 13: Microbial Genetics (Ch 8) - Mt. SACfaculty.mtsac.edu/cbriggs/Microbial Genetics Ch 8 FA16.pdf · Microbial Genetics (Ch 8) Author: Briggs, Christopher M. Created Date: 10/31/2016

Fig. 8.18

Mutations

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Fig. 8.21

Mutations

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Fig. 8.23

Ames test for mutagens

Page 16: Microbial Genetics (Ch 8) - Mt. SACfaculty.mtsac.edu/cbriggs/Microbial Genetics Ch 8 FA16.pdf · Microbial Genetics (Ch 8) Author: Briggs, Christopher M. Created Date: 10/31/2016

en.wikipedia.org

Cyclophosphamide: a nitrogen mustard alkylating agent

Page 17: Microbial Genetics (Ch 8) - Mt. SACfaculty.mtsac.edu/cbriggs/Microbial Genetics Ch 8 FA16.pdf · Microbial Genetics (Ch 8) Author: Briggs, Christopher M. Created Date: 10/31/2016

Fig. 8.25

Griffith’s experiment: a sign of recombination

Page 18: Microbial Genetics (Ch 8) - Mt. SACfaculty.mtsac.edu/cbriggs/Microbial Genetics Ch 8 FA16.pdf · Microbial Genetics (Ch 8) Author: Briggs, Christopher M. Created Date: 10/31/2016

Fig. 8.26

Transformation

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Fig. 8.27

Conjugation

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Fig. 8.29

Recombination: Transduction

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Fig. 8.30

Plasmids

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Fig. 8.13

Operon model