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Sessions: • Gram stain and Bacteriology

• Bacillus, diphteroids,staph,and strept under the microscope

• Medias

• Sensitivity test

• Normal flora bacteria and throat culture

• Urine and stool culture

• Agglutination and precipitation tests

• Acid fast culture and stain, and yeast, candida, and Aspergillus.

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Gram stain and Bacteriology Bacillus, diphteroids, staph, and strept under the

microscope

Streptococcus pneumoniae gram postive ,diplococci, lancet shape

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Niesseriae Gram negative, diplococci, Kidney shaped

Niesseriae Spp. Intracellular

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Diphteroids Gram positive , Chinese letters ( rods)

Bacillus spp. Gram positive , rods, there are spores

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Staphylococci

Streptococci

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Hemophilus spp G-ve cocbacilli

Mixed gram stain Staph & Proteus

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Bacillus on blood agar dry colonies

Microbiological Midea

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Blood agar for –ve and +ve bacteria

Contains blood cells from an animal (e.g. a sheep); most bacteria will grow on this medium , but it is NOT Suitable for Student Use due to potential for contamination from human contact. Btw….. it so advantageous for distinguishing the pathogenic and the normal bacteria by noticing the Decomposition RBC which indicate to the pathogenic bacteria.

Alpha-type Hemolytic Streptococcus it turns into green.. alpha hemolytic >green beta hemolytic > yellow gamma hemolytic > no hemolysis

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Beta Hemolytic Bacteria it turns to yellow

The picture on the left is group A Beta hemolytic which is sensitive to Bacitracin and The picture on the right is group B Beta hemolytic which is resistanat to Bacitracin.

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MacConky Agar for some -ve

a culture medium designed to grow Gram-negative bacteria so it's a selective media** and we can called it indicator media **, also it is used for distinguishing between lactose fermental bacteria which appears pink in color and lactose none-fermental bacteria that is appears yellow in the same media .

CLED agar a selective media for the gram positive & it is used for differentiations and enumeration of microorganisms in urine and to distinguish between Staphylococcus aureus which produces green colonies on this medium and the staphulococcus intermedius which produces yellow colonies on the same medium .

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Hecton Enteric agar

Hecton enteric agar media it’s selective for salmonella and shegella , its color is yellowish to orange salmonella growth turns the color into blue shigella growth turns its color into green

TCBS

TCBS :Thiosulfate-citrate-bile salts-sucrose agar or TCBS agar is a type of selective agar culture plate that is used in microbiology laboratories to isolate Vibrio spp. TCBS Agar is highly selective for the isolation of Vibrio cholera

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kligler’s iron agar… is red in color, solid put these test tubes in the autoclave to make sure they are sterile after that we should tilt them and till the agar gets solid. SIM agar… we use this colorless media to show three things:H2S production ,, indole +/- and motility

Kigller’s agar Citrate +ve>> blue -ve >>green

Tubidity test (Motility)

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Sensitivity test

A plate containing Alpha hemolysis streptococcus viridans which is resistance to the antibiotic Optochin

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Normal flora bacteria and throat culture

Throat swab

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Staphylococcus Aureus culture on CLED agar at the bottom ( yellow) Staphylococcus epidermidis culture on CLED agar at the bottom ( white)

Staphylococcus Aureus culture on CLED agar ( yellow)

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Staphylococcus epidermidis culture on CLED agar ( white)

MSA (MANNITOL SALT AGAR): is a selective media for Gram positive staphylococci & Micrococcaceae The original color of the media is pink/red. - Staphylococcus Aureus (Coagulase +ve) turns the color to yellow -Staphylococcus Epidermidis (Coagulase -ve ) the color remains pink

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Dephtheroid it appears dry on a plate

Urine Culture

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E coli

E coli on Blood “ I guess “!!

E coli on CLED

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E Coli on MacConky

Enterobacter and Ecoli

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Klebsialla on Blood

Klebsialla is non-motile , weak fructose fermenter, and its colonies have a very mucoid appearance and that because this bacteria has a capsule

Klebs on MacConky

Klebsialla

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Stool Culture

Salmonella

a growth of salmonella with a lot of H2S; (black dots inside the colony)

Hikton Enteric agar

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Shigella

shegella colonies appears transparent

Hikton Enteric agar

Proteus

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In blood culture , it is highly motile , its make wavy appearance (the wavy appearance on media is called swarming phenomena).It has a bad smell.

TCBS media ,its color is green , if there is growth for vibrio ,the plate color is turned from green to golden(the color is changed

due to sucrose fermentation ).

Vibrio Cholera

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pseudomonas on cled has metalic appearence +silver color

Pseudomonas it has a good smell, its color is silver

Shigella E coli H2S Production

SIM agar The 1st one on the right >> H2S production the 2nd one >> Indole positive

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Proteus +ve and –ve

Collection of diff. tubes

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Agglutination and precipitation tests

counter-immuno electrophoresis(Qualitative method)

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Rocket electrophoresis(both qualitative & quantitative)

Double immune-diffusion technique(Qualitative method)

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single radial immune diffusion both methods

Agglutination in pregnancy test

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Antibody antibiotic reaction

Acid fast culture and stain, and yeast, candida, and Aspergillus.

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TB culture

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With gram stain

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Asperigellus nigra

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