METHODOLOGYOn 7th April 2016 student Bachelor environmental healthcare semester 6 has

visited Sungai Tekala Hulu Langat Selangor for Assessment of river water quality.

head for Sungai Tekala Recreational Forest which is located about 14km away from

the town of Semenyih in the district of Hulu Langat, Selangor. The distance from

Kuala Lumpur is approximately 50km. The coordinates and map Sungai Tekala is

N03 03.62’ E101 52.13’.The water source is s stream and for waterfall profile is

cascade & Vertical Fall. There are several sampling point areas that were

identified/selected by our group for this study at the Sungai Tekala Area. The

identified/selected sampling points are at :-

Sampling point A : Upper stream

Sampling point B : Middle Stream

Sampling point C: Downstream

Measure the water quality as an in-situ measurement of the following parameters:

Temperature

pH

Dissolve Oxygen (DO)

DO percentage

Electrical conductivity

Total Dissolve Solid (TDS)

WATER SAMPLING POINT MAPPING

Legend and Direction

Tree Waterfall

Road/Path

b) Procedure during collection and handling of water sample.

1) Hold the bottle near its bottom and submerge it to a depth of about 20 cm,

with the mouth facing slightly downwards

2) Before collecting any sample, we are at the right place/point. The are was

clean from non homogeneous pieces of detritus, such as leaves, in the

sample. Avoid touching and disturbing the bottom of a water body when

taking a depth sample, because this will cause particles to become

suspended.

3) Sampling depth is measured from the water surface to the middle of the

sampler.

4) A bottle that is to be used for transport or storage of the sample should be

rinsed three times with portions of the sample before being filled. This does

not apply if the storage/transport bottle already contains a preservative

chemical.

5) At the same time, actual coordinates for each sampling station by using

Global Positioning System (GPS) was determine.

6) After taking the water, a colilert was added in the bottle

7) At any time that the sample bottles are not closed, their tops must be kept in a

clean place.

8) All measurements taken in the field must be recorded in the field notebook

before leaving the sampling station.

9) All supporting information should be recorded in the field notebook before

leaving the sampling station. These notes and observations will be of great

help when interpreting analytical results.

10)Samples should be transferred to sample bottles immediately after collection if

they are to be transported. If analysis is to be carried out in the field, it should

be started as soon as possible.

C) Procedure for transportation, storage and preservation of water samplebefore analysis.

1) After taking the sample from all sampling point the bottle was label with

different tag.

2) All samples was placed in a ice box to cooled in ice shortly collection.

3) The sample was storing in the ice box with a suitable period time

4) Before all the sample was keep in the ice box, the colilert was added in all the

samples.

5) The samples been preserved to make sure the transport of the samples in a

condition that not an effect on their bacteria and avoid from contamination.

6) After the all samples was arrival at the laboratory, the samples was incubated

for 24 hours.

d) Procedure to analyse water samples (determination of microbial

presence).

1) The time between sample collection and analysis should, in general, not

exceed 6 hours, and 24 hours is considered the absolute maximum.

2) It is assumed that the samples are immediately placed in a lightproof

insulated box containing melting ice or ice-packs with water to ensure rapid

cooling.

3) It is imperative that samples are kept in the dark and that cooling is rapid. If

these conditions are not met, the samples should be discarded.

4) Transfer all the bottle into the incubator to incubate for 24hours.

5) Counting the number of colonies grown after a defined incubation time

provides a general estimation of the bacteriological load in the water samples.

6) After 24hours,the result was collected from laboratory .