METHOD AND RESULTS 3. IN VIVO
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METHOD AND RESULTS 3. IN VIVO • DISCUSSION Christoph Fusch, Dpt. Of Pediatrics, McMaster University, Hamilton, Ontario, L8S 4K1, Canada, Phone: +1 (905) 521 2100 ext 75721, E-mail: [email protected] METHOD 1, 2: FABRICATION OF SOU/ LAD AND IN VITRO GAS EXCHANGE EXPERIMENTAL SETUP SOU Body: • Polydimethylsiloxane (PDMS) • Vascular network of micro channels • Low volume of 0.14mL • Collect blood samples pre- and post SOU for gas analysis flow rate: 0.5 – 4 mL/min Gas Permeable Membrane: • Polycarbonate (PC),normal PDMS, or porous PDMS • Surface area of 14.08cm 2 “Lung Assist Device for term and preterm newborns with respiratory failure- Artificial Placenta ” A. Manan 1 , N. Rochow 2 , W. Wu 3 , G. Fusch 2 ,S. Monkman 2 , PR. Selvaganapthy 3 , C. Fusch 1,2 1 Dpt. of Biomedical Engineering, 2 Div. of Neonatology, Dpt. of Pediatric, 3 Department of Mechanical Engineering, McMaster University INTRODUCTION • Respiratory failure still accounts for 5% of neonatal deaths. Mechanical ventilation is associated with long term complications. Based on the natural in utero placenta, we propose a pump less lung assist device (LAD) with low priming volume, low resistance, and high gas exchange. OBJECTIVE ACKNOWLEDGMENT • CHRP Funding Received • Provisional US Patent is filed, No. 61526288 • Rochow et al, 2013. Int J Artif Organs • Wen-I-W et al, 2013. Lab on Chip • C. Fusch holds the Hamilton Health Sciences Foundation Jack Sinclair Chair in Neonatology at McMaster University RESULTS 1: IN VITRO GAS EXCHANGE OF SOU Postnatal: artificial placenta • Porous PDMS was superior to PC, normal PDMS membranes by demonstrating a1.6x- 2.6x higher O 2 and 1.2x- 2.4x higher CO 2 exchange rate. RESULT 2: IN VITRO GAS EXCHANGE OF LAD • 1. In vitro: Optimize SOU, by testing gas exchange rates of different membrane. 2. In vitro: Evaluate gas exchange rates of LAD. 3. In vivo: Assess feasibility of LAD in hypoxic piglet model. 5 10 15 20 30 40 0 0.0005 0.001 0.0015 0.002 0.0025 0.003 O2 Transfer per cm2 Flowrate (mL/min) O2 Transfer (mL/ min/cm2) 5 10 15 20 30 40 0 0.002 0.004 0.006 0.008 0.01 0.012 0.014 0.016 0.018 CO2 Transfer per cm2 Flowrate (mL/min) CO2 Transfer (mL/min/cm2) 5 10 15 20 30 40 0 10 20 30 40 50 60 Resistance of bundle Flow Rate (mL/min) Resistance (mmHg) 0 10 20 30 40 50 60 0 50 100 150 200 250 Flow rate vs. Heart Rate Flow rate (mLmin) Heart Rate 0 10 20 30 40 50 60 0 20 40 60 80 100 120 Flowrate vs. Blood Pressure Flow rate (mL/min) Blood Pressure • Achieved a maximum flow rate of 57mL/minute • No correlation is observed between flow rate and heart rate/ blood pressure, indicating that no additional stress is imposed on piglet by the LAD. T=2 sec T=7 sec T=18 sec • Bundle can successfully support 10% neonatal lung function. • 20-30% of CO 2 is successfully released, and with increasing flow rate results in improved CO 2 removal. Single Oxygenator Unit Lung Assist Device Newborn piglet 3-4 hours old • Successfully constructed a low volume LAD. • PDMS membranes was most effective for O 2 and CO 2 transfer. • In vitro experiments indicate that the LAD can potentially provide support equivalent to 10% of neonatal lung function. • In vivo experiments indicate that flow rates through device are tolerated by the cardiovascular system. • Future work: Assess gas exchange performance of LAD in a hypoxic piglet model, improve biocompatibility, and develop special catheters to improve cannulation time. membrane PDMS body channel • Porous PDMS have a 2x lower resistance than PC and 1.3x lower resistance than normal PDMS membrane.
description
T=2 sec. T=7 sec. T=18 sec. “Lung Assist Device for term and preterm newborns with respiratory failure- Artificial Placenta ” Manan 1 , N. Rochow 2 , W. Wu 3 , G. Fusch 2 ,S. Monkman 2 , PR. Selvaganapthy 3 , C. Fusch 1,2 - PowerPoint PPT Presentation
Transcript of METHOD AND RESULTS 3. IN VIVO
METHOD AND RESULTS 3. IN VIVO
•
DISCUSSION
Christoph Fusch, Dpt. Of Pediatrics, McMaster University, Hamilton, Ontario, L8S 4K1, Canada, Phone: +1 (905) 521 2100 ext 75721, E-mail: [email protected]
METHOD 1, 2: FABRICATION OF SOU/ LAD AND IN VITRO GAS EXCHANGE EXPERIMENTAL
SETUPSOU Body: • Polydimethylsiloxane (PDMS)• Vascular network of micro
channels• Low volume of 0.14mL
• Collect blood samples pre- and post SOU for gas analysis flow rate: 0.5 – 4 mL/min
Gas Permeable Membrane: • Polycarbonate (PC),normal
PDMS, or porous PDMS• Surface area of 14.08cm2
“Lung Assist Device for term and preterm newborns with respiratory failure- Artificial Placenta ”
A. Manan1, N. Rochow2, W. Wu3, G. Fusch2,S. Monkman2, PR. Selvaganapthy3, C. Fusch1,2
1Dpt. of Biomedical Engineering, 2Div. of Neonatology, Dpt. of Pediatric, 3Department of Mechanical Engineering, McMaster University
INTRODUCTION• Respiratory failure still accounts for 5% of neonatal deaths.
Mechanical ventilation is associated with long term complications. Based on the natural in utero placenta, we propose a pump less lung assist device (LAD) with low priming volume, low resistance, and high gas exchange.
OBJECTIVE
ACKNOWLEDGMENT
• CHRP Funding Received• Provisional US Patent is filed, No. 61526288• Rochow et al, 2013. Int J Artif Organs• Wen-I-W et al, 2013. Lab on Chip• C. Fusch holds the Hamilton Health Sciences Foundation
Jack Sinclair Chair in Neonatology at McMaster University
RESULTS 1: IN VITRO GAS EXCHANGE OF SOU
Postnatal: artificial placenta
• Porous PDMS was superior to PC, normal PDMS membranes by demonstrating a1.6x- 2.6x higher O2 and 1.2x- 2.4x higher CO2 exchange rate.
RESULT 2: IN VITRO GAS EXCHANGE OF LAD
• 1. In vitro: Optimize SOU, by testing gas exchange rates of
different membrane.2. In vitro: Evaluate gas exchange rates of LAD.3. In vivo: Assess feasibility of LAD in hypoxic piglet model.
5 10 15 20 30 400
0.0005
0.001
0.0015
0.002
0.0025
0.003O2 Transfer per cm2
Flowrate (mL/min)
O2
Tran
sfer
(mL/
min
/cm
2)
5 10 15 20 30 400
0.002
0.004
0.006
0.008
0.01
0.012
0.014
0.016
0.018CO2 Transfer per cm2
Flowrate (mL/min)
CO
2 Tr
ansf
er (m
L/m
in/c
m2)
5 10 15 20 30 400
10
20
30
40
50
60Resistance of bundle
Flow Rate (mL/min)
Res
ista
nce
(mm
Hg)
0 10 20 30 40 50 600
50
100
150
200
250Flow rate vs. Heart Rate
Flow rate (mLmin)
Hea
rt R
ate
0 10 20 30 40 50 600
20
40
60
80
100
120Flowrate vs. Blood Pressure
Flow rate (mL/min)
Blo
od P
ress
ure
• Achieved a maximum flow rate of 57mL/minute• No correlation is observed between flow rate and heart rate/
blood pressure, indicating that no additional stress is imposed on piglet by the LAD.
T=2 sec T=7 sec T=18 sec
• Bundle can successfully support 10% neonatal lung function.
• 20-30% of CO2 is successfully released, and with increasing flow rate results in improved CO2 removal.
Single Oxygenator Unit Lung Assist Device
Newborn piglet 3-4 hours old
• Successfully constructed a low volume LAD.• PDMS membranes was most effective for O2 and CO2
transfer.• In vitro experiments indicate that the LAD can potentially
provide support equivalent to 10% of neonatal lung function.• In vivo experiments indicate that flow rates through device
are tolerated by the cardiovascular system.• Future work: Assess gas exchange performance of LAD in
a hypoxic piglet model, improve biocompatibility, and develop special catheters to improve cannulation time.
membrane
PDMS body
channel
• Porous PDMS have a 2x lower resistance than PC and 1.3x lower resistance than normal PDMS membrane.
