Metabolism: Energy and Enzymes
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Transcript of Metabolism: Energy and Enzymes
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Metabolism: Energy and Enzymes
Chapter 6
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Energy = the ability to do workKinetic Energy - energy of motionPotential Energy - stored energy
First Law of Thermodynamics - the law of conservation of energySecond Law of Thermodynamics - energy cannot be changed without a loss of usable energy (heat)
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Metabolic Reactions
Reactants Products a + b c + d
Exergonic: releases energy Endergonic: absorbs energy
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ATP - the energy currency of cells (adenosine triphosphate)
Functions:1. CHEMICAL WORK - Supplies energy needed to make macromolecules that make up the cell (and organism)2. TRANSPORT WORK - Supplies energy needed to pump substances across the cell membrane3. MECHANICAL WORK - supplies energy needed to make muscles contract and other cellular parts to move (flagella)
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Metabolic Pathways & Enzymes (6-3)
Enzyme - protein molecule that functions as an organic catalyst to speed reactions
Substrate - reactants in the enzymatic reaction, this is what an enzyme attaches to
Energy of Activation - the energy required to cause the reaction
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Properties of Enzymes:
•Enzymes are made of proteins.
•They speed up chemical reactions inside the cytoplasm.
•They are needed only in small amounts
•They remain unchanged after each reaction and can therefore be reused
•Each enzyme is specific for a substrate
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Induced Fit Model - substrates and enzymes fit together like a lock and key. (Degradation vs Synthesis) breaking down vs building
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Factors Affecting Enzymatic Speed1. Substrate concentration2. Temperature & pH *3. Enzyme concentration
Enzymes can be denatured - they change shape so much that they are no longer effective. High temp or pH can cause denaturation.
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Siamese cats have an enzyme that works at lower temperatures only, causing the nose and ears to become a darker color than the rest of the body.
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Enzymatic Inhibition - when a substance binds to an enzyme and decreases its activity. (Usually reversible)
Two types: Competitive Inhibition Noncompetitive Inhibition (allosteric site)
**Both are forms of feedback inhibition
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Some inhibitors are NOT reversible - poisons like cyanide, lead poisoning all affect enzymes
QUESTION: What type of inhibition is pictured below?
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Enzyme Quiz
http://www.sciencegeek.net/Biology/review/U2Enzymes.htm
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Enzyme Lab(s) 2H2O2 ----> 2H2O + O2
Hydrogen Peroxide is broken down by the enzyme: catalase within cells.
KMNO4 (potassium permanganate) can be used to determine how
much H2O2 is left within the flask after the reaction has stopped. (Titration)
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The more KMnO4 you use, the more peroxide is left in the flask.
You slowly add KMnO4, which is purple, to the flask. The peroxide in the flask causes the KMnO4 to lose color when the solution is mixed thoroughly. When all the peroxide has reacted with KMnO4, any additional KMnO4 will remain light brown or pinkish even after you swirl the mixture. This is the endpoint. Record the amount of KMnO4 you have used.
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The activity of catalase can be seen by the bubbling of oxygen during the reactionGenerally speaking, catalase reactions occur faster at warmer temperatures.
If temperature is too hot (boiling) then the enzyme becomes denatured
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Virtual Labs with Enzymes
McGraw Hill LabBio 114 EnzymesLab Bench Enzyme Catalysis
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Use the following vocabulary in your discussionsFunctional group Carbon ringsCarboxyl group Phosphate groupAmino group EnzymeHydroxyl group SubstrateDouble bond MonosaccharideSingle bond DisaccharideC-C bonds Fatty AcidsNucleic acid SteroidsProteinLipidCarbohydrateAmino AcidNitrogen base