Mendelian Genetics. Vocabulary Heredity Genetics Trait Gamete Pollination Fertilization Law of...
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Transcript of Mendelian Genetics. Vocabulary Heredity Genetics Trait Gamete Pollination Fertilization Law of...
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Mendelian Genetics
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VocabularyHeredityGeneticsTraitGametePollinationFertilization Law of segregation Law of independent assortment
Dominant
Recessive
Hybrid
Phenotype
Genotype
Homozygous
Heterozygous
Allele
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Genetics
• Scientific study of heredity
• Heredity-passing of traits from parents to offspring
• Trait -characteristic that is inherited
• Many had realized family resemblance but didn’t know how
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Gregor Mendel
•Austrian monk
•1822-1884
•Came up with the laws of heredity
•Importance of work not realized until 1900
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Mendel’s Work
• First to succeed in predicting how traits would be transferred from one generation to the next
• Used garden peas (reproduced sexually via gametes)
• Pollination -transfer of male pollen grains to the pistil of flower (female)
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Pollination
• Results in fertilization (fusion of male and female gametes)
• Pea plants normally went through self-pollination (gametes from self)
• Mendel needed to cross pollinate
• Taking pollen from one plant and using it to fertilize another plant
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Monohybrid Crosses
• Crosses where the parents only differed by one trait (mono)
• Hybrid -offspring formed by parents having different forms of a trait
• First trait he looked at was height
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First Generation
• Six-foot-tall pea plant that came from a population of pea plants, all of which were over six feet tall
• Short pea plant that was less than two feet tall and which came from a population of pea plants that were all short
• Cross-pollinated the two plants
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Result
• All of the offspring grew to be as tall as the taller parent
• It was as if the shorter parent had never existed
• Ratio of 4:0
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Second Generation
• Allowed the tall plants in this first generation to self-pollinate
• Counted more than 1000 plants in this second generation
• ¾ of the plants were tall• ¼ of the plants were short• Ratio of approximately three tall
plants to one short plant (3:1)
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Generations
• Original parents, the true-breeding tall and short plants, are known as the P1 generation (P- parent)
• Cross of P1 are known as the F1 generation (F- filial or son/daughter)
• Cross two F1 plants with each other equals F2 generation
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Further Experimentation
• Did similar monohybrid crosses with a total of seven pairs of traits
• Studying one pair of traits at a time
• In every case, he found that one trait of a pair seemed to disappear in the F1 generation, only to reappear unchanged in one-fourth of the F2 plants
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Genes
• Located on chromosomes, units of heredity
• The rule of unit factors
• Each organism has two factors that control each of its traits
• Genes exists in alternative forms
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Alleles
• Alternative forms of a gene for each variation of a trait of an organism
• Could have 2 alleles for tallness
• 2 alleles for shortness • 1 allele for tallness and 1 for
shortness • Receive 1 allele from each
parent
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The Rule of Dominance
• One gene in a pair masks another
• Dominant -observed trait of an organism that masks the recessive form of a trait
• Recessive -trait of an organism that can be masked by the dominant form of a trait
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How to Write the Alleles
• Each gene is expressed as a letter
• Dominant allele is expressed as a uppercase letter
• Recessive allele is expressed as a lower case letter
• Previous example: T for tall, t for short
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Law of Segregation
• 1. There are alternative forms for genes.
• 2. For each characteristic or trait organisms inherit two alternative forms of that gene, one from each parent. These alternative forms of a gene are called alleles.
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Law of Segregation
• 3. When gametes (sex cells) are produced, allele pairs separate or segregate leaving them with a single allele for each trait.
• 4. When the two alleles of a pair are different, one is dominant and the other is recessive.
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Phenotypes and Genotypes
• What was the difference between the tall plants in P1 and the tall plants in F1?
• Two organisms, therefore, can look alike but have different underlying gene combinations
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Phenotype
• Outward appearance of an organism, regardless of its genes
• How the organism looks
• A tall TT plant and a tall Tt plant look what?
• Their phenotype would be tall
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Genotype
• Combination of genes in an organism
• Does the tall TT plant and the tall Tt plant have the same genotype?
• Can’t always tell an organism’s genotype by looks
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Homozygous vs. Heterozygous
• Homozygous- when there are two identical alleles for a trait
• TT and tt
• Heterozygous- when there are two different alleles for a trait
• Tt
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Law of Independent Assortment
• Genes for different traits are inherited independently of each other
• For example, seed shape and seed color
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Punnett Squares
• Shorthand way of finding the expected proportions of possible genotypes in the offspring of a cross
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How to Work Punnett Square
• Step #1: Determine the genotypes of the parent organisms
• Step #2: Write down your "cross" (mating). Write the genotypes of the parents in the form of letters (ex: Tt x tt)
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Step #3: Draw a p-square
T is the allele for being tall and t is the allele for being short, cross one plant that is homozygous recessive (tt) with another that is heterozygous tall (Tt).
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Step #4: "Split" the letters of the genotype for each parent & put them "outside" the p-square
t t
T
t
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Step #5: Determine the possible genotypes of the offspring by filling in the p-square
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