MEMBRANE STRUCTURE AND FUNCTION CHAPTER 7 1. WHAT YOU MUST KNOW: Why membranes are selectively...
Transcript of MEMBRANE STRUCTURE AND FUNCTION CHAPTER 7 1. WHAT YOU MUST KNOW: Why membranes are selectively...
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MEMBRANE STRUCTURE AND FUNCTION
CHAPTER 7
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WHAT YOU MUST KNOW:
• Why membranes are selectively permeable.
• The role of phospholipids, proteins, and carbohydrates in membranes.
• How water will move if a cell is placed in an isotonic, hypertonic, or hypotonic solution.
• How electrochemical gradients are formed.
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CELL MEMBRANE
A. Plasma membrane is selectively permeable
• Allows some substances to cross more easily than others
B. Fluid Mosaic Model
• Fluid: membrane held together by weak interactions• Mosaic: phospholipids, proteins, carbs
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EARLY MEMBRANE MODEL• (1935) Davson/Danielli –
Sandwich model• phospholipid bilayer between
2 protein layers• Problems: varying chemical
composition of membrane, hydrophobic protein parts
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THE FREEZE-FRACTURE METHOD: REVEALED THE STRUCTURE OF
MEMBRANE’S INTERIOR
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FLUID MOSAIC MODEL
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PHOSPHOLIPIDS• Bilayer• Amphipathic =
hydrophilic head, hydrophobic tail• Hydrophobic barrier:
keeps hydrophilic molecules out
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MEMBRANE FLUIDITY• Low temps: phospholipids
w/unsaturated tails (kinks prevent close packing)• Cholesterol resists
changes by:
• limit fluidity at high temps• hinder close packing at
low temps• Adaptations: bacteria in hot
springs (unusual lipids); winter wheat ( unsaturated phospholipids) 9
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MEMBRANE PROTEINSIntegral Proteins• Embedded in
membrane• Determined by freeze
fracture• Transmembrane with
hydrophilic heads/tails and hydrophobic middles
Peripheral Proteins• Extracellular or
cytoplasmic sides of membrane• NOT embedded• Held in place by the
cytoskeleton or ECM• Provides stronger
framework
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Integral & Peripheral proteins11
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TRANSMEMBRANE PROTEIN STRUCTURE
Hydrophobic interior
Hydrophilic ends
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Some functions of membrane
proteins
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CARBOHYDRATES
• Function: cell-cell recognition; developing organisms• Glycolipids, glycoproteins• Eg. blood transfusions are type-specific
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SYNTHESIS AND SIDEDNESS OF MEMBRANES
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SELECTIVE PERMEABILITY
• Small molecules Small molecules (polar or nonpolar) cross easily (hydrocarbons, hydrophobic molecules, CO2, O2)
• Hydrophobic core prevents passage of ionsions, , large polar molecules large polar molecules
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PASSIVE TRANSPORT
NO ENERGY needed!Diffusion down concentration gradientconcentration gradient (high
low concentration)Eg. hydrocarbons, CO2, O2, H2O
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OSMOSIS: DIFFUSION OF
H2O
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EXTERNAL ENVIRONMENTS CAN BE EXTERNAL ENVIRONMENTS CAN BE HYPOTONICHYPOTONIC, , ISOTONICISOTONIC OR OR HYPERTONICHYPERTONIC TO TO
INTERNAL ENVIRONMENTS OF CELLINTERNAL ENVIRONMENTS OF CELL
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FACILITATED DIFFUSION
Transport proteinsTransport proteins (channel or carrier proteins) help hydrophilic substance cross
(1) Provide hydrophilic channel or (2) loosely bind/carry molecule across
Eg. ions, polar molecules (H2O, glucose)
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AQUAPORIN: CHANNEL PROTEIN THAT ALLOWS PASSAGE OF H2O
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GLUCOSE TRANSPORT PROTEIN (CARRIER PROTEIN)
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ACTIVE TRANSPORT
Requires ENERGYENERGY (ATP)Proteins transport substances against concentration gradientconcentration gradient (low high conc.)
Eg. Na+/K+ pump, proton pump
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ELECTROGENIC PUMPS: GENERATE VOLTAGE ACROSS MEMBRANE
Na+/K+ Pump• Pump Na+ out, K+ into
cell• Nerve transmission
Proton Pump• Push protons (H+)
across membrane• Eg. mitochondria (ATP
production)
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COTRANSPORT: MEMBRANE PROTEIN ENABLES “DOWNHILL” DIFFUSION OF ONE
SOLUTE TO DRIVE “UPHILL” TRANSPORT OF OTHER
Eg. sucrose-H+ cotransporter (sugar-loading in plants)
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PASSIVE VS. ACTIVE TRANSPORT
• Little or no Energy• High low
concentrations• DOWN the
concentration gradient• eg. diffusion,
osmosis, facilitated diffusion (w/transport protein)
• Requires Energy (ATP)• Low high
concentrations• AGAINST the
concentration gradient• eg. pumps,
exo/endocytosis
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BULK TRANSPORT• Transport of proteins, polysaccharides,
large molecules
Endocytosis: take in macromolecules, form new vesicles
Exocytosis: vesicles fuse with cell membrane, expel contents
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TYPES OF ENDOCYTOSISPhagocytosis:“cellular eating” - solids
Pinocytosis:“cellular drinking” - fluids
Receptor-Mediated Endocytosis:Ligands bind to specific receptors on cell surface
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