Material Safety Data Sheet - Prozomix MSDS (NZY... · NZYTech, Lda. INOVISA, Instituto Superior de...

NZYTech, Lda. INOVISA, Instituto Superior de Agronomia, Tapada da Ajuda, 1349-017 Lisboa, Portugal. Tel: +351.213643514 Fax: +351.213630284 Internet www.nzytech.com

Material Safety Data Sheet 1/4 According to Directives 91/155/CEE and 1999/45/Ec Issue date: November 2008

Buffer A1 (Re-suspention buffer), for NZYMiniprep

1. IDENTIFICATION OF THE SUBSTANCE/PREPARATION AND OF THE COMPANY/UNDERTAKING

Identification of the substance or preparation

Catalogue references: MB01001 (50 columns); MB01002 (200 columns)

Product name: Buffer A1 (Re-suspention buffer), for NZYMiniprep

Company/undertaking identification

NZYTech, Lda., INOVISA, Instituto Superior de Agronomia, Tapada da Ajuda,

1349-017 Lisboa, Portugal, Tel. +351.213643514, Fax +351.213630284, www.nzytech.com

Emergency telephone number

PT: INEM, Centro de Informação Anti-Venenos, Rua Infante D. Pedro, 8

1749-075 Lisboa, Portugal, Tel. +351.217950143/44/46

Outside Portugal (PT): Call your regional Poisons information Service or call local Life Saving Service.

2. COMPOSITION/INFORMATION ON INGREDIENTS

Substance/preparation: Solution, consisting of the following ingredients:

Ingredient name CAS number EC number % Classification

Water 7732-18-5 231-791-2 > 50 -

Other ingredients: Ingredients not listed here are not dangerous or their concentrations do not exceed the limits

specified in the EU directive 1999/45/EC.

See section 16 for the full text of the R phrases.

Occupational exposure limits, if available, are listed on section

3. HAZARDS IDENTIFICATION

Hazard description: Not applicable.

Information concerning particular hazards for human and environment: The product does not have to be labelled

due to the calculation procedure of the “General Classification guideline for preparations of the EU” in the

latest valid version.

Classification system: The classification is according to the latest editions of the EU-lists, and extended by company

and literature data.

See section 11 for more detailed information on health effects and symptoms.

4. FIRST-AID MEASURES

General information: No special measures required.

Inhalation: Supply fresh air; consults doctor in case of complaints.

Ingestion: If swallowed, wash out mouth with water provided person is conscious. If symptoms persist consult a

doctor.

Skin contact: Wash skin with soap and copious amount of water. Generally the product does not irritate the skin.

Eye contact: Rinse opened eye for several minutes under running water.




Protection of first-aiders: No action shall be taken involving any personal risk or without suitable training. It may be

dangerous to the person providing aid to give mouth-to mouth resuscitation.


5. FIRE-FIGHT MEASURES

Extinguishing media:

suitable – use an extinguish agent suitable for the surrounding fire.

not suitable – none known.

Special exposure hazards: No specific fire or explosion hazard. Promptly isolate the scene by removing all persons

from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or

without suitable training.

Special protective equipment for fire-fighters: Fire-fighters should wear appropriate protective material and self-

contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.

6. ACCIDENTAL RELEASE MEASURES

Personal precautions: Not required.

Measures for environmental protection: Dilute with plenty of water. Do not allow to enter sewers/surface or

ground water.

Methods for cleaning up: Bind any escaping liquid with universal binder and dispose.

7. HANDLING AND STORAGE

Handling: No special measures required.

Storage: Store in accordance with local regulations. Store in original container protected from direct sunlight in a

dry, cool and well-ventilated area at max 25 °C, away from incompatible materials (see section 10) and food

and drink. The original product package of NZYTech allows a safe storage. Keep packages tightly closed and

sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to

prevent leakage. Do not store in unlabelled containers. Use appropriate containment to avoid environmental

contamination.

Packaging materials – recommended: Use original container.

8. EXPOSURE CONTROLS/PERSONAL PROTECTION

Exposure controls

General protective and hygienic measures: The usual precautionary measures are to be adhered to when handling

chemicals.

Respiratory protection: Not necessary.

Hand protection: Chemical-resistant, impervious gloves complying with an approved standard should be worn at all

times when handling chemical products if a risk assessment indicates this is necessary.

Eye protection: Not necessary.

Skin protection: Not necessary.

Environmental exposure controls: Emissions from ventilation or work process equipment should be checked to

ensure they comply with the requirements of environmental protection legislation. In some cases, fume

scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce

emissions to acceptable levels.




9. PHYSICAL AND CHEMICAL PROPERTIES

Appearance

Physical state: Liquid.

Colour: Colourless.

Odour: Odourless.

Important health, safety and environmental information

Boiling point: Undetermined.

Melting point: Undetermined.

Flash point: Undetermined.

Explosive properties: Not considered to be a product presenting a risk of explosion.

Vapour pressure: Undetermined.

Relative density: Undetermined.

Solubility: Fully miscible.

10. STABILITY AND REACTIVITY

Stability: The material is stable. Decomposition is not observed during expiration period under recommended

conditions.

Materials to avoid: No specific data.

Conditions to avoid: No specific data.

11. TOXICOLOGICAL INFORMATION

Potential acute health effects

Inhalation: No irritant effect.

Ingestion: Not observed.

Skin contact: No irritant effect.

Eye contact: No irritant effect.

Carcinogen/mutagen effects:

Other effects:

Result species dose exposure

LC50 Oral Rat > 2000 mg/kg -

LD50 Oral Rat > 2000 mg/kg - 12. ECOLOGICAL INFORMATION

Environmental effects: Slightly hazardous for water. Do not allow undiluted product or large quantities of it to

reach ground water, water course or sewage system.

13. DISPOSAL CONSIDERATIONS

Methods of disposal: The generation of waste should be avoided or minimised wherever possible. Dispose of

surplus and non-recyclable products via a licensed waste disposal contractor. Disposal of this product,

solutions and any by-products should at all times comply with the requirements of environmental protection

and waste disposal legislation and any regional local authority requirements. Avoid dispersal of spilt material

and runoff and contact with soil, waterways, drains and sewers.

Hazardous waste: No.




14. TRANSPORT INFORMATION

International transport regulations: Not classified.

15. REGULATORY INFORMATION

EU regulations:

The product is not subject to identification regulations according to EU Directives 67/548/EEC and 1999/45/EC

(including amendments) and takes into account the intended product use.

Product use: Industrial applications.

Europe inventory: all components are listed or exempted.

Other EU regulations: Not available.

16. OTHER INFORMATION

Notice to reader: The information and recommendations contained herein are to the best of our knowledge

reliable. However, nothing herein is to be construed as a warranty or representation in respect of safety in

use, suitability efficiency or otherwise, including freedom from patent infringements. Users should make their

own tests for their particular purpose. NZYTech cannot accept any liability for any loss, damage or

infringement arising from the use of the information or recommendations contained herein.

MSDS has been prepared in accordance with EU directives 67/548/EEC, 1907/2006 EC and 1999/45/EC and other

European Union legislation in force.


Material Safety Data Sheet 1/5 According to Directives 91/155/CEE and 1999/45/EC Issue date: November 2008

Buffer A2 (Lysis buffer), for NZYMiniprep



Catalogue reference: MB01001 (50 columns); MB01002 (200 columns)

Product name: Buffer A2 (Lysis buffer), for NZYMiniprep







Outside Portugal (PT): Call your regional Poisons Information Service or call local Life Saving Service.




Water 7732-18-5 231-791-2 > 50

Sodium dodecyl sulphate 151-21-3 205-788-1 1-2.5 % Xi; R36/38

Xn; R21/22

Sodium hydroxide 1310-73-2 215-185-5 < 1% C; R35






Hazard description: The preparation is classified as dangerous according to Directive 1999/45/EC and its

amendments.

Classification system: Xi; R36/38; Xn; R21/22; C; R35.

Human health hazards: Irritating to eyes and skin. Harmful in contact with skin and if swallowed. Causes severe

burns.



Inhalation: If inhaled, remove to fresh air. Get medical attention if symptoms occur.

Ingestion: Do not ingest. If potentially dangerous quantities of this material have been swallowed, call a doctor

immediately. Get medical attention if symptoms appear.

Skin contact: Flush contaminated skin with plenty of water and with soap. Get medical attention if symptoms appear.

Eye contact: In case of contact with eyes, rinse immediately with plenty of water. Get medical attention if irritation

occurs.












from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without

suitable training.




Personal precautions: Do not breathe vapour. Wear suitable protective gloves and eye protection. Regular staff

training is necessary, indicating hazards and precautions on the basis of operating instructions.

Measures for environmental protection: Dilute with plenty of water. Do not allow to enter sewers/surface or ground

water.

Methods for cleaning up: Bind any escaping liquid with universal binder and dispose in accordance to local

regulations for disposal of hazardous chemicals. Clean any contaminated equipment and floors with plenty of

water.


Handling: Ensure good ventilation/exhaustion at the workplace. Prevent formation of aerosols.

Storage: Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,

cool and well-ventilated area at max 25 °C, away from incompatible materials (see section 10) and food and

drink. The original product package of NZYTech allows a safe storage. Keep packages tightly closed and sealed

until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent

leakage. Do not store in unlabelled containers. Use appropriate containment to avoid environmental

contamination.



Exposure limits values: Not available.

Exposure controls

Occupational exposure controls: Use only with adequate ventilation. Use process enclosures, local exhaust

ventilation or other engineering controls to keep worker exposure to airborne contaminants below any

recommended or statutory limits.

Respiratory protection: Use a properly fitted, air-purifying or air-feed respirator complying with an approved

standard if a risk assessment indicates this is necessary. Respirator selection must be based on known or

anticipated exposure levels, the hazards of the product and the safe working limits of the selected respirator.






Eye protection: Safety eyewear complying with an approved standard should be used when a risk assessment

indicates this is necessary to avoid exposure to liquid splashes, mists, gases or dusts.

Skin protection: Personal protective equipment for the body should be selected based on the task being performed

and the risks involved and should be approved by a specialist before handling this product. Wash hands,

forearms and face thoroughly after handling chemical products, before eating, smoking and using the

lavatory and at the end of the working period. Appropriate techniques should be used to remove potentially

contaminated clothing. Wash contaminated clothing before reusing. Ensure that eyewash stations and

safety showers are close to the workstation location.





Personal hygiene: Eating, drinking, smoking, taking snuff and storage of food in work areas and at outdoor

workplaces is prohibited. Avoid contact with skin, eyes and clothing. Wash hands thoroughly with soap and

water when stopping work and before eating, and apply a protective skin cream.


Appearance


Colour: Colourless.

Odour: Odourless.




Flash point: Not applicable.


Relative density (at 20 °C): < 1.02 g/cm3


pH value at 20 °C: > 11.0


Stability: The material is stable.


Conditions to avoid: No dangerous reactions known.






Inhalation:

Ingestion:

Skin contact: Irritant effect to skin and mucous membranes.

Eye contact: Irritant effect.


Effects after multiple expositions: Not observed.

Corrosive effects: Not observed.

Additional toxicological information: The product shows the following dangers according to the calculation

method of the General EU Classification Guidelines for Preparations as issued in the latest version: Irritant.

Other effects:

Result species dose substance

LC50 Oral Rat > 500 mg/kg NaOH

LD50 Oral Rat > 500 mg/kg NaOH 12. ECOLOGICAL INFORMATION


reach ground water, water course or sewage system. Hazardous to drinking water, if large amounts enter the

subsoil and waters.







Hazardous waste: yes (if large amounts).


Additional information:

Class code: C5, LQ 7 = 5l/30Kg

EmS: F-A, S-B.

Regulatory

information

UN number Proper shipping name Class Packing

group

ADR/RID Class 1824 Sodium Hydroxide

solution

8 III

IMDG Class 1824 Sodium Hydroxide

solution

8 III

IATA-DGR Class 1824 Sodium Hydroxide

solution

8 III


Material Safety Data Sheet 5/5 According to Directives 91/155/EEC and 1999/45/EC Issue date: November 2008



EU regulations:

Classification and labelling have been determined according to EU Directives 67/548/EEC and 1999/45/EC (including

amendments) and take into account the intended product use.

Hazards symbols:

Xi - Irritant

Risk phrases: R36/38 – Irritating to eyes and skin.

Safety phrases: S23 – Do not breathe gas/fumes/vapour/spray.

S26 – In case of contact with eyes, rinse immediately with plenty of water and seek

medical advice.

S37 – Wear suitable gloves.

S60 – This material and its container must be disposed of as hazardous waste.

S45 - In case of accident or if you feel unwell, seek medical advice immediately (show label of

bottle where possible)





Full text of R-phrases referred to in sections 2 and 3 – Europe:

R21/22 – Harmful in contact with skin and if swallowed.

R35 – Causes severe burns.

R36/38 – Irritating to eyes and skin.

Full text of classifications referred to in sections 2 and 3 – United Kingdom (UK)

Xi - Irritant

Xn- harmful

C- Corrosive










Buffer A3 (Neutralization buffer), for NZYMiniprep




Product name: Buffer A3 (Neutralization buffer), for NZYMiniprep










Ingredient name CAS number EC number EU number % Classification

Water 7732-18-5 231-791-2 > 50

Guanidine chloride 50-01-1 200-002-3 607-148-00-0 25-50 Xn R36/38

Xi; R22







amendments.

Classification system: Xn; R36/38; Xi; R22.

Human health hazards: Irritating to eyes and skin. Harmful if swallowed.



General information: Symptoms of poisoning may even occur after several hours; therefore medical observation for

at least 48 hours after the accident.






occurs.













suitable training.



Additional information: The substance itself is not flammable. Product package burns like paper or plastic. Cool any

undamaged containers in water, and remove from the danger zone if possible.





water.



water.








contamination.



Exposure limits values: Not available.

Exposure controls




























Appearance


Colour: Colourless.

Odour: Acidic.









Stability: The material may not be stable under certain conditions of storage or use.


Conditions to avoid: No dangerous reactions known.






Inhalation: Harmful.

Ingestion: Harmful.



Acute toxicity

Product /Ingredient name: Guanidinium chloride

test species Route Result

LD50 Rat Oral 475 mg/kg

LD Rabbit Dermal > 2 g/kg

LDLo Rat Subcutaneous 404 mg/kg

Potential chronic health effects

Chronic effects: No known significant effects or critical hazards.

Carcinogenicity: No known significant effects or critical hazards.

Mutagenicity: No known significant effects or critical hazards.

Reproductive toxicity: No known significant effects or critical hazards.

Development effects: No known significant effects or critical hazards.

Fertility effects: No known significant effects or critical hazards.

Over-exposure signs/symptoms

Inhalation: No known significant effects or critical hazards.

Ingestion: No known significant effects or critical hazards.

Skin: Adverse symptoms may include the following: Irritation, redness.

Eyes: Adverse symptoms may include the following: Irritation, redness, watering.

Target organs: Contains material which causes damage to the following organs – central nervous system (CNS).


methods of the General EU Classification Guidelines for Preparations as issued in the latest version: Harmful;

Irritant.

12. ECOLOGICAL INFORMATION



subsoil and waters.














EU regulations:



Hazards symbols:

Xn - Harmful

Hazard determining components of labelling: Guanidine chloride

Risk phrases: R22 – Harmful if swallowed.




medical advice.

S36/37 – Wear suitable protective clothing and gloves.









R22 – Harmful in contact with skin and if swallowed.



Xn - Harmful

Xi- Irritant










Buffer A4 (Wash buffer), for NZYMiniprep




Product name: Buffer A4 (Wash buffer), for NZYMiniprep











Water 7732-18-5 231-791-2 > 50 -

















doctor.











Suitable – use an extinguish agent suitable for the surrounding fire.

Not suitable – none known.









ground water.









contamination.



Exposure controls


chemicals.














Appearance


Colour: Colourless.

Odour: Odourless.











conditions.









Sensitization: No sensitization effects known.


Environmental effects: Generally not hazardous for water.














EU regulations:
















Buffer AE (Elution buffer), for NZYMiniprep




Product name: Buffer AE (Elution buffer), for NZYMiniprep











Water 7732-18-5 231-791-2 > 50 -

















doctor.





















ground water.









contamination.



Exposure controls


chemicals.














Appearance


Colour: Colourless.

Odour: Odourless.











conditions.











Environmental effects: Generally not hazardous for water.














EU regulations:
















Buffer AW (Wash buffer), for NZYMiniprep




Product name: Buffer AW (Wash buffer), for NZYMiniprep










Ingredient name CAS number EC number EU number % Classification

Water 7732-18-5 231-791-2 > 50

Guanidine chloride 50-01-1 200-002-3 607-148-00-0 25-50 Xn R36/38

Xi; R22

Ethanol 64-17-5 200-578-6 603-002-00-5 10-25 F; R11







amendments.

Classification system: Xn; R36/38; Xi; R22; F; R11.

Human health hazards: Irritating to eyes and skin. Harmful if swallowed. Highly flammable.



General information: Symptoms of poisoning may even occur after several hours; therefore medical observation for

at least 48 hours after the accident.









occurs.










suitable training.









water.



water.








contamination.



Occupational exposure limits

Ethanol: WELL Long-term value: 1920 mg/m3, 1000 ppm.

Exposure controls









anticipated exposure levels, the hazards of the product and the safe working limits of the selected

respirator.



















Appearance


Colour: Colourless.

Odour: Alcohol-like.





Explosive properties: Not considered to be a product presenting a risk of explosion. However, formation of

explosive air/vapour mixtures is possible.




Stability: The material may not be stable under certain conditions of storage or use.


Conditions to avoid: Reacts with acids, alkalis and oxidizing agents.









Acute toxicity

Product /Ingredient name: Guanidinium chloride



LD Rabbit Dermal > 2 g/kg

LDLo Rat Subcutaneous 404 mg/kg

Product /Ingredient name: Ethanol



LC50 (4 hours) Rat Inhalativel 20000 mg/l


Chronic effects: No known significant effects or critical hazards.




Development effects: No known significant effects or critical hazards.

Fertility effects: No known significant effects or critical hazards.




Skin: Adverse symptoms may include the following: Irritation, redness.

Eyes: Adverse symptoms may include the following: Irritation, redness, watering.

Target organs: Contains material which causes damage to the following organs – central nervous system (CNS).


methods of the General EU Classification Guidelines for Preparations as issued in the latest version: Harmful;

Irritant.




subsoil and waters.












International transport regulations: Not applicable.


EU regulations:



Hazards symbols:

F - Flammable Xi – Irritant/Xn – Harmful

Hazard determining components of labelling: Guanidine chloride; Ethanol solution.

Risk phrases: R22 – Harmful if swallowed.


R11 – Highly flammable


S26 – In case of contact with eyes, rinse immediately with plenty of water and seek medical

advice.





S7 – Keep container tightly closed.

S16 – Keep away from sources of ignition – No smoking.


Europe inventory: All components are listed or exempted.







R22 – Harmful in contact with skin and if swallowed.


R11 - Highly flammable


Xn - Harmful

Xi- Irritant

F - Flammable










Collecting Columns, for NZYMiniprep




Product name: Collecting Columns, for NZYMiniprep









Substance/preparation: Preparation

Within the present knowledge of the supplier, this product does not contain any hazardous ingredients in

quantities requiring reporting in this section, in accordance with EU or national regulations.

Statement of hazardous nature: To the best of our knowledge this substance/preparation is not classified

as hazardous according to EU; US, or any other know national regulations.


Appearance

Physical state: solid.







This is not an MSDS. According to the EU and US regulations we are not required to supply an MSDS for a product

which is not classified as hazardous.



NZYTech Spin Columns, for NZYMiniprep




Product name: NZYTech Spin Columns, for NZYMiniprep









Substance/preparation: Substance, cross-linked dextran (biopolysaccharide), crosslinked with epichlorohydrine,

particle size 40-160 µm (C6H12O6)n.

Chemical formula: (C6H12O6)n. EC number: 232-677-5

CAS number: 9004-54-0

Classification: this is not a hazardous substance/preparation.





















Suitable – use an extinguish agent suitable for the surrounding fire.

Not suitable – none known.















contamination.



Exposure controls







Appearance

Physical state: solid.






















Other effects:

Result species dose exposure

LC50 Oral Rat 3 g/kg -

LD50 Oral Mic 12 g/kg - 12. ECOLOGICAL INFORMATION

Environmental effects: Not applicable.











EU regulations:
















RNase A, for NZYMiniprep




Product name: RNase A, for NZYMiniprep (MB01001 - 5 mg; MB01002 - 20 mg)











Glycerol 56-81-5 200-289-5 > 50 Xi; R36/37/38







amendments.

Classification system: Xi; R36/37/38.

Human health hazards: Irritating to eyes, respiratory system and skin.



Inhalation: If inhaled, remove to fresh air. Get medical attention if symptoms appear.





occurs.











Special exposure hazards: No specific fire or explosion hazard.






Personal precautions: Immediately contact emergency personnel. Keep unnecessary personnel away. Use suitable

protective equipment.

Measures for environmental protection: Avoid dispersal of split material and runoff and contact with soil,

waterways, drains and sewers.

Methods for cleaning up: If emergency personnel are unavailable, contain spilt material and place in an appropriate

container for disposal. For small spills, add absorbent, scoop up material and place in a sealable, liquid-proof

container for disposal. For large spills, dykes spilt material or otherwise contain material to ensure runoff does

not reach a waterway. Place spilt material in an appropriate container for disposal.








contamination.



Ingredient name: Glycerol.

Exposure controls


























water when stopping work and before eating, and the apply protective skin cream.


Appearance


Colour: Colourless.

Odour: Odourless

Odour threshold: The lowest known value is 4400 ppm (Glycerol).


Boiling point: The lowest known value is 290 °C (554 °F).

Melting point: May start to solidify at 20 °C (68 °F) based on data for: Glycerol.


Vapour pressure: The highest known value is 0.0004 kPa (0.003 mm Hg) (at 20 °C) (Glycerol)

Relative density: The only known value is 1.261 (water = 1) (Glycerol).

Solubility: easily soluble in cold water, hot water. Insoluble in diethyl ether.

Viscosity: Kinematics: The highest known value is 1412 cSt (Glycerol).

Vapour density: The highest known value is 3.1 (air= 1) (Glycerol).



Materials to avoid: Reactive or incompatible with the following materials: oxidising materials and moisture.






Inhalation: Irritating to respiratory system.


Skin contact: Irritant effect to skin

Eye contact: Irritant effect to eyes.

Acute toxicity

Product/Ingredient name: Glycerol

Result species dose

LD50 Oral Rat 12600 mg/kg

LD50 Oral Mouse 4090 mg/kg

LD50 Oral Guinea pig 7750 mg/kg

LD50 Dermall Rabbit >18700 mg/kg








Skin: No known significant effects or critical hazards.

Target organs: Contains material which causes damage to the following organs: kidneys, upper respiratory tract,

skin, eye, lens or cornea.


Ecotoxicity data:

Product/Ingredient name: Glycerol.

Result Species exposure

54000 mg/ml Oncorhynchus mykiss (LC50) 96 hours

> 10000 mg/ml Daphnia magna (EC50) 24 hours

Other ecological information

Persistence/Biodegradability:

Product/Ingredient name: Glycerol.

Aquatic half-life Photolysis Biodegradability

- > 60%; 28 day(s) Readily

Other adverse effects: no known significant effects or critical hazards.














EU regulations:



Hazards symbols:

Xi - Irritant

Hazard determining components of labelling:

Risk phrases: R36/37/38 – Irritating to eyes, respiratory system and skin.



medical advice.










R36/37/38 – Irritating to eyes, respiratory system and skin.


Xi- Irritant








Material Safety Data Sheet - Prozomix MSDS (NZY... · NZYTech, Lda. INOVISA, Instituto Superior de...

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Transcript of Material Safety Data Sheet - Prozomix MSDS (NZY... · NZYTech, Lda. INOVISA, Instituto Superior de...