Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid...
Transcript of Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid...
![Page 1: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/1.jpg)
Master Slide
From Model Systems to Clinical Trials in Phelan-McDermid Syndrome
Alexander Kolevzon, MD Clinical Director, Seaver Autism Center
Director, Child and Adolescent Psychiatry
Mount Sinai Health System
![Page 2: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/2.jpg)
Disclosures of Potential Conflicts Source Research
funding Advisor/ consultant
Employee Speakers’ Bureau
Books, Intellectual Property
In-Kind Services
Stock of Equity
Honorarium
Seaver
Foundation
X
NIH/NINDS X
New York Community
Trust
X
Simons Foundation
X
American Psychiatric Publishing
X
Amo Pharma
X
Coronis X
Ovid X
5AM Ventures
X
*Mount Sinai and Joseph Buxbaum hold a shared patent for IGF-1 in Phelan-McDermid syndrome The following presentation contains information concerning a use that has not been approved by the U.S. Food and Drug Administration
sema4 X
Labcorp X
![Page 3: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/3.jpg)
Developmental Synaptopathies Consortium
To comprehensively characterize PMS
using standard medical, behavioral, and
cognitive measures.
Track the natural history using repeated
longitudinal assessments
Develop biomarkers and novel,
naturalistic measures for objective
assessment.
Evaluate safety, tolerability, and feasibility
of novel therapeutics.
![Page 4: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/4.jpg)
PMS
Pathophysiology
Drug Development
Novel Therapeutics
Gene Discovery (SHANK3)
Model Systems (e.g., mice; rats; human neurons)
From Model Systems to Clinical Trials
![Page 5: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/5.jpg)
![Page 6: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/6.jpg)
![Page 7: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/7.jpg)
![Page 8: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/8.jpg)
![Page 9: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/9.jpg)
Costales & Kolevzon, 2016
![Page 10: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/10.jpg)
A Double-Blind Placebo-Controlled Crossover Trial of IGF-1 in Children with
Phelan-McDermid Syndrome
Placebo Placebo
IGF-1 IGF-1
12 weeks 12 weeks
wash-out
4 weeks
![Page 11: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/11.jpg)
Dose depends on
weight and is twice a day with food
Finger stick glucose levels are
monitored before
each dose
28 gauge ½ inch needle
![Page 13: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/13.jpg)
Change in scores at week 12 in social withdrawal and restricted behavior
![Page 14: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/14.jpg)
Change in scores at week 12 in hyperactivity
![Page 15: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/15.jpg)
Change in scores at week 12 in sensory reactivity
![Page 16: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/16.jpg)
Potential Side Effects
In clinical studies with 71 subjects over 3.9
years, 5% or more patients had the following
side effects:
Hypoglycemia with loss of
consciousness/seizures
Tissue swelling
Bruising
Ear infection
Snoring/tonsillar hypertrophy
Headache
Dizziness
Convulsions
Vomiting
Cardiac murmur
Arthralgia
Pain in extremities
Thymus hypertrophy (immune system organ
behind the sternum/chest)
In 19 patients with PMS, 2 or more patients
had the following side effects:
Hypoglycemia
Tissue swelling
Bruising at injection site
Constipation
Increased appetite
Sleep disturbance
Irritability
Decreased energy
Increased chewing/biting
![Page 17: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/17.jpg)
![Page 18: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/18.jpg)
![Page 19: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/19.jpg)
![Page 20: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/20.jpg)
Placebo
OXT
OXT
12 weeks 12 weeks
![Page 21: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/21.jpg)
Visit Baseline Week 4 Week8 Week 12 Week 16 Week 20 Week 24 Week 28
Phase Double Blind Open Label
Medical/Psych History
X - - - - - - -
EEG X - - X - - - X
Eye Tracking X X X X X X X x
VEP X X X X X X X X
CGI X X X X X X X X
SAND X X X X X X X X
ADOS-2 X - - X - - X X
Mullen X - - X - - X -
PEP X - - X - - X -
Vineland X - - X - - X -
ADI X - - - - - - -
Parent forms X X X X X X X X
Study Drug Administration
X X X X X X - -
OXT Study Procedures
![Page 22: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/22.jpg)
OXT Potential Side Effects
• Headache
• Rapid heart rate
• Slow heart rate
• Nausea/vomiting
• Irregular heartbeat
• Rash
• Elevated blood pressure
• Mood changes / irritability
• Weight gain
• Insomnia
• Agitation / aggression
• Hyperactivity
Syntocinon® Package Insert
Syntocinon® (oxytocin) is a synthetic, (1-6) cyclic nonapeptide.
Chemically, oxytocin is designated as Glycinamide, L-cysteinyl-L-
tyrosyl-L-isoleucyl-L-glutaminyl-L-asparaginyl-L-cysteinyl-L-prolyl-
L-leucy1-, cyclic (1-6)-disulfide.
The structural formula is:
Syntocinon® (oxytocin) injection is provided as a sterile solution
for intravenous or intramuscular administration. Each 1 mL of
solution contains 10 USP or International Units of oxytocin and
the following inactive ingredients:
acetic acid, NF, qs to ………………….. pH 4 ± 0.3 alcohol, USP………………………. 0.61 % by vol. chlorobutanol, NF……….,………………. 0.5%
sodium acetate, USP …,…………………… 1 mg
sodium chloride, USP …………………… 0.017 mg
water for injection, USP, qs to ………………… 1 mL
![Page 23: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/23.jpg)
An open-label study to investigate the safety, tolerability and efficacy of a 6-hour intravenous infusion of AMO-01 to treat adolescents and
adults with Phelan-McDermid syndrome and epilepsy
C28H34N2O4
MW = 462.25
![Page 24: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/24.jpg)
Ras-ERK Pathway Inhibitor
• The Mitogen Activated Protein Kinases (MAPKs) are critical mediators of neuronal survival and synaptic plasticity:
– AMO-01 targets activation of the MAPK Extracellular signal–Regulated Kinase (ERK)
TYROSINE KINASE
RECEPTOR
Ras
MLK Raf
MEK
ERK
TRANSCRIPTION
WITHIN
NUCLEUS
AMO-01 PREVENTS
ANCHORING AND ACTIVATION OF RAS
![Page 25: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/25.jpg)
Ras-ERK Pathway in PMS
• The activity of the Ras-ERK pathway was assayed in various tissues of the shank3 knockout (KO) transgenic mouse model of PMS.
• Levels of phosphorylated ERK (pERK) used an index of activation.
– Ras-ERK pathway activity was markedly increased in the cortex of the shank3 KO mouse. In contrast, Akt was only slightly activated
– The Ras-ERK pathway was also activated in lymphocytes of shank3 KO mice
WT KO WT KO
Hippocampus Cortex
p
ER
K ½
WT KO
Activation of the Ras-ERK pathway in
Wild Type (WT) and shank3 knockout
(KO) mice as indexed by pERK levels in
hippocampus and cortex
Peripheral activation of the Ras-ERK
pathway in Wild Type (WT) and shank3
knockout (KO) mice as indexed by pERK
levels in lymphocytes
![Page 26: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/26.jpg)
AMO-01 in Shank3-knockout mouse
• A single dose of 30 mg/kg i.p. AMO-01 or placebo was administered to a
shank3 KO mouse model of PMS (N=10) or controls;
• 30 mg/kg i.p. produces plasma levels of AMO-01 that have been readily
achieved in human subjects in clinical studies in the oncology area;
• AMO-01 rescued multiple aspects of the PMS phenotype:
– Increased anxiety (light dark box test)
– Excessive grooming (skin lesions)
– Impaired social recognition and response to social novelty
– Sensory-motor dysfunction (beam walking)
– Species typical behavior deficits (marble burying)
– Seizure threshold (audiogenic seizures)
![Page 27: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/27.jpg)
Study Aims
• To evaluate the safety and tolerability of a single 6-hour intravenous
infusion of AMO-01
• To evaluate the efficacy of AMO-01 in reducing seizure frequency by at
least 25 percent as measured by a caregiver completed seizure diary
• To evaluate the efficacy of AMO-01 as measured by clinician-
completed rating scales, caregiver completed diaries, functional
assessments and biomarker assessments
![Page 28: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/28.jpg)
Inclusion Criteria
• Diagnosis of PMS
• Age 12-45 at screening
• Diagnosis of epilepsy with a
witnessed seizure event in the
28 days prior to screening, and
a minimum of 4 seizures monthly in the 6 months prior
to screening
Exclusion Criteria
• Medications and therapies must
remain stable within 4 weeks
prior to screening until the last
study assessment
• Known hypersensitivity to
farnesylated dibenzodiazepinone
or any of the formulation
components
• History of uncontrolled
hypotension or hypertension
• Coumadin or heparin in the 2 weeks prior to screening
![Page 29: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/29.jpg)
Challenges for Clinical Trial Readiness
![Page 30: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/30.jpg)
Solution: Biomarkers
“A characteristic that is objectively measured and evaluated as an indicator
of normal biological processes,
pathogenic processes, or pharmacologic
responses to a therapeutic intervention.” – Biomarkers Definitions Working Group, 2001
Behavior
Biomarkers
Genes
![Page 31: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/31.jpg)
Biomarkers: Examples
Genes
Brain function (MRI, EEG)
Brain structure (MRI)
Eye tracking
Proteins
Heart rate
Blood levels
Pupil response
Behavior
Biomarkers
Genes
![Page 32: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/32.jpg)
Biomarkers: Key Features
Objective
Reliable
Quantifiable
Sensitive
![Page 33: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/33.jpg)
Diagnosis and screening
Treatment effectiveness
Early efficacy
Target engagement
Stratification Treatment
Biomarker
Diagnosis Autism
Profile A
Treatment A; Medium Dose
Treatment A; Higher Dose
Profile B
Treatment B
Biomarkers: Uses
![Page 34: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/34.jpg)
Electrophysiological Markers
Identify subtypes of neurodevelopmental disorders based on
excitatory/inhibitory (E/I) profiles
Inform personalized treatment approaches
Monitor treatment response and determine optimal responders
Identify associations between electrophysiological responses
and clinical outcomes
P. Siper, PhD J. Foss-Feig, PhD
![Page 35: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/35.jpg)
Transient Visual Evoked Potentials (VEP)
Siper, PM
Phelan-McDermid
syndrome (PMS)
Typically developing
controls
![Page 36: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/36.jpg)
From Preclinical Models to Clinical Trials
![Page 37: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/37.jpg)
Improvement in VEPs after IGF-1 in PMS
n=6; p=.048
Siper, PM
![Page 38: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/38.jpg)
Clinician-administered observation and corresponding caregiver interview capturing DSM-5 sensory reactivity symptoms in children with
neurodevelopmental disorders
Sensory Assessment for Neurodevelopmental Disorders (SAND)
Siper, PM et al., 2017
![Page 39: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/39.jpg)
Association between VEP and sensory reactivity
Siper, PM
![Page 40: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/40.jpg)
Effects of IGF-1 on Sensory Reactivity
n=6, p = .037
Siper, PM
![Page 41: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/41.jpg)
VEP in PMS and Idiopathic Autism
A subset of ~30% of iASD
patients fall within 1 SD of the
PMS P60-N75 mean and are
defined as “PMS-like”
Siper, PM Foss-Feig, J
![Page 42: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/42.jpg)
IGF-1 in Idiopathic Autism
![Page 44: Master Slide - 22q13 · Master Slide From Model Systems to Clinical Trials in Phelan-McDermid Syndrome Alexander Kolevzon, MD ... Change in scores at week 12 in social withdrawal](https://reader035.fdocuments.in/reader035/viewer/2022081600/60462f612553847afd7cc83e/html5/thumbnails/44.jpg)
Acknowledgments
Seaver Center Team • Joseph Buxbaum • Paige Siper • Danielle Halpern • Pilar Trelles • Ting Wang • Michelle Gorenstein • Jennifer Foss-Feig • Yitzchak Frank • Reymundo Lozano • Hala Harony-Nicolas • Silvia De Rubeis • Elodie Drapeau • Michael Breen • Sven Sandin
PMS Consortium • Latha Soorya • Elizabeth Berry-Kravis • Audrey Thurm • Jon Bernstein • Craig Powell
Neuropsych Group • Deborah Pearson • Thomas Frazier
Boston Children’s Team • Mustafa Sahin • April Levin • Chuck Nelson