MARKER ASSISTED SELECTION

PLANT GENETICS RECITATIONMarch 8th 2012

Why marker assisted selection?

MARKER ASSISTED SELECTION

•Individuals carrying the trait of interest are selected based on a marker which is linked to the trait and not on the trait itself.

•Indirect selection.

•Useful when the trait is difficult to measure, and/or is evident only at late developmental stages.

Winter Facult. Spring

Kom

polt

i

Nur

e

OSU

11

Stri

der

Igri

Dic

ktoo

88A

b536

Tre

moi

s

Mor

ex

Har

ring

ton

ZCCT-H

HvSnf2

Tri

umph

SNP2 SNP3 SNP4 BstUI/HhaI Fnu4HI 1R.OWB.Aglu4.T7 AACTTATACGCGCCCAGCCGGACGCCGGTCATCGGCAAGGTGGTCTTCATGGGGCTGCAG 2R.OWB.Aglu4.T7 AACTTATACGCGCCCAGCCGGACGCCGGTCATCGGCAAGGTGGTCTTCATGGGGCTGCAG 1OWBD.Aglu4.T7 AACTCATACGCGCCCAGTCGGACGCCGGTCATCGGCAAGGTGGTCTTCATGGGGATGCAG 2OWBDAglu4.T7 AACTCATACGCGCCCAGTCGGACGCCGGTCATCGGCAAGGTGGTCTTCATGGGGATGCAG **** ************ ************************************ *****

Kurtford iBison 1H+4H+5H

I I I K I K I I K

The barley stripe rust resistance story ~ 1987

BARLEY STRIPE RUST

www.barleyworld.org

BARLEY STRIPE RUST

•Caused by fungus Puccinia striiformis f. sp. hordei

•Common in Mexico and South America but usually not in Oregon.

•It first appeared in the US in 1991

•When infection is severe, losses of 50% are common

BARLEY STRIPE RUST•Resistant cultivars are available

•It is necessary to develop resistant cultivars adapted to different barley growing areas

•Resistance to the disease is a quantitative trait (non-Mendelian inheritance)

•Three Quantitative Trait Loci (QTL) for stripe rust resistance have been mapped and their effects validated.

•There are molecular markers located in the target regions of the three QTL

1 2 3 4 5 6 7 8 9 10 11 12 Isolate ID Origin Topper Heils

Franken Emir Astrix Hiproly Vanrunda Abed

Binder 12 Trumpf Mazurka Bigo I 5 Bancroft Virulence Formula PSH race

Int-061 Peru 9 8 2 7 8 8 9 9 3 8 2 9 1,2,4,5,6,7,8,10,12 New Int-062 Peru 9 7 7 8 8 8 9 9 6 8 2 8 1,2,3,4,5,6,7,8,9,10,12 New Int-048 Peru 9 7 6 8 7 8 9 9 8 7 2 4 1,2,3,4,5,6,7,8,9,10,12 New

Race characterization of BSR isolates from Huancayo, Peru. 2007

Cali-sib x BowmanShyri x Galena CI10587 x Galena

BSR-45 D1-72 D3-6

Harrington Baronesse

Orca

BCD DB

BCD47 BCD12 D3-6/B23 D3-6/B61

AJO BU OPS

iBISON and BISON

iBISON BISON

BCD14340DsT-667Act8A12RbgMD18MWG837B22scind0004625ABC165C26Bmac039929GBM100730BCD09836GBM104248BG36701354Bmag021158BG36994061GBM105168ABC16073JS10C86Bmac0144A87MWG706A96KFP170101Blp111ABC261119MWG2028121KFP257B122WMC1E8130MWG912133ABG387Ascssr04163scssr08238

136

1H

DsT-10ABG0585scind026227

ABG00817

scssr1022636scssr0775939GBM106642Pox45scssr0338156scssr12344scssr02236Ebmac0684

63

BCD1434.265ABG35668GBM102371scsnp0334383vrs188Bmag012594DsT-4197MWG503102GBM1062103KFP203104MWG882A108ABG1032117ABG072124Ebmc0415137cnx1139Zeo1149GBM1019161Aglu5F3R2163MWG720165GBM1012170wst7173scssr08447179MWG949A180

2H

BCD9070

ABC171A26GBM107430scssr1055933MWG798B36Dst-2739BCD70642DsT-3958alm61Bmac020966ABC32569DsT-6773

scssr2569187ABG37789Bmag022598

Act8C121ABG499124GBM1043125

scsnp23255151ABG004155

scind02281166MWG883172

DsT-24181

HVM62190

DsT-40199

ABC172scssr25538212

DsT-35218

3H

MWG6340MWG07721HVM4024DsT-2929CDO54230CDO12231hvknox335Dhn639ABC303scssr2056941

CDO79544HVM349DST-46scind03751scssr18005

50

Tef252GBM102060Bmag035362scind1045567DsT-7974scssr14079ABG47280

GBM105983KFP22192Ebmac070194MWG652B95GBM1048101Hsh111HVM67112KFP241.1116ABG601124

4H

scssr023060MWG6186DsT-68ABC48311ABG61012

ABG39537scssr0250344scssr1807645Bmac009653NRG045A55scsnp0426056Ale58

ABC30279scind1699182scssr1533485scsnp0614490srh100

scssr05939111

RSB001A120

scsnp001771280SU-STS1134ABG003B141

scssr10148157Tef3166MWG877169BE456118A170ABG496179scsnp02109E10757A193

ABG391197JS10B198ABC622205DsT-33207Bmag0113C215MWG602A223scssr03907224scssr03906225

5H

MWG6200Bmac0316scssr093984

MWG652A31MWG602B35scind6000242JS10A45GBM102151GBM106861BG29929765HVM3168rob70Bmag0009scssr0209371

ABG47481Bmac0218C88ABG38892scsnp2122699MWG820101GBM1008122scssr05599123MWG934126scind04312b132scssr00103GBM1022135

Bmac0040143DsT-18145DsT-32B146DsT-22152DsT-28159scind60001DsT-74160

MWG514162MWG798A163DsT-71167

6H

ABG7040Bmag000714scind0069420AW98258029MWG089CDO47536

ABG38038BE60207344scssr0797057scsnp0046066ABC25568ABC165D69HvVRT273scssr1586482GBM103086scsnp22290MWG808DAK642scind00149

89

scsnp00703MWG203197

RSB001C98nud103lks2115ABC1024117Bmag0120125DsT-30126WG380B127ABC310B137Ris44139

ABG461A167WG380A171GBM1065178

HVM5196scssr04056KFP255197

ThA1199

7H

Disease severity of barley stripe rust resistance QTL isolines. 2006

Accession Huancayo, Peru

Toluca, Mexico

Corvallis, USA

Mean ± std. error

1H 20 45 20 28 ± 8.3 4H 5 1 20 9 ± 5.8 5H 40 60 50 50 ± 5.8 7H 0 1 1 1 ± 0.3 1H + 4H 10 1 20 10 ± 5.5 1H + 5H 50 20 40 37 ± 8.8 4H + 5H 15 33 70 39 ± 16.2 1H + 4H + 5H 0 1 0 0.3 ± 0.3 BCD12 (1H) 15 1 15 10 ± 4.7 BCD 47 (4H + 5H) 15 25 15 18 ± 3.3 Baronesse 50 80 80 70 ± 10 CI10587 (7H) 10 1 5 5 ± 2.6

P-O

PA

1 +

2 m

ap

ch cM BA

RO

NE

SS

E

BIS

ON

1H

BIS

ON

4H

BIS

ON

1H+

4H

BIS

ON

5H

BIS

ON

1H+

5H

BIS

ON

4H+

5H

BIS

ON

1H+

4H+

5H

BIS

ON

7H

BC

D12

BC

D47

1_1513 4H 71.250 a a a a a a a a a a a2_0207 4H 75.671 a a a a a a a a a a a1_0309 4H 75.671 a a a a a a a a a a b2_1332 4H 79.006 a a a a a a a a a a b1_0170 4H 81.204 a a a a a a a a a a a1_1526 4H 82.291 a a a a a a a a a a a1_1213 4H 83.378 a a a a a a a a a a a2_0765 4H 84.465 a a a a a a b a a a b1_1398 4H 84.465 a a a a a a a a a a a2_0732 4H 91.373 a a a b a a b a a b b1_1168 4H 92.460 a a a a a a a a a a a1_0785 4H 93.547 a a a a a a a a a a a

EBmac701 4H 95.786

1_1292 4H 96.854 a a b b a a b b a a b2_0838 4H 96.854 a a a a a a a a a a a2_0454 4H 99.052 a a a a a a a a a a a

baal29j181_1194 4H 104.585 a a a a a a a a a a a1_0712 4H 109.082 a a a a a a a a a a a1_1235 4H 110.169 a a a a a a a a a a a2_0883 4H 111.256 a a a a a a a a a a a1_0697 4H 112.343 a a a a a a a a a a a2_0224 4H 113.430 a a a a a a a a a a a2_0760 4H 114.517 a a a a a a a a a a a2_0007 4H 115.604 a a a a a a a a a a a1_0610 4H 115.604 a a a a a a a a a b b2_0932 4H 116.685 a a a a a a a a a a a

OWB

Chr. 4H

ORO

DEFD

FEF

BB

A

DEDE

C

A

010203040506070

Bar

ones

se 1H 4H

1H+

4H 5H

1H+

5H

4H+

5H

1H+

4H+

5H 7H

BC

D12

BC

D47

0 2 4 6 8

10

12

14

HVM40

HVM03Bmac181

Bmac030B

GBM1071MWG2180

Bmac310

EBmac635

EST4535*k07229baal29j18HvMLO3*k03352

GBM1015

Bmag419HvSnf2

HdAmyB

1_1292 –EST4535

0 2 4 6 8

10

12

14

HVM40

HVM03Bmac181

Bmac030B

GBM1071MWG2180

Bmac310

EBmac635

EST4535*k07229baal29j18HvMLO3*k03352

GBM1015

Bmag419HvSnf2

HdAmyB

1_1292 –EST4535

1_1292 –EST45351_1292 –EST4535

EBmac701

baal29j18SSR markers used for MAS

*

P-O

PA

1 +

2 m

ap

ch

cM

BA

RO

NE

SS

E

BIS

ON

1H

BIS

ON

4H

BIS

ON

1H+

4H

BIS

ON

5H

BIS

ON

1H+

5H

BIS

ON

4H+

5H

BIS

ON

1H+

4H+

5H

BIS

ON

7H

BC

D1

2

BC

D4

7

2_0275 1H 1.087 a b a b a a a b a b a 1_1311 1H 6.775 a a a a a a a a a a a 1_0410 1H 7.862 a a a a a a a a a a a 1_0636 1H 8.949 a a a a - a a a a - - 1_1011 1H 11.147 a a a a a a a a a a a 2_1226 1H 14.432 a b a b a a a b a b a 2_0712 1H 33.230 a b a b a a a b a b b 1_0760 1H 46.833 a a a b a a a a a b b 1_0814 1H 47.920 a a a b a a a a a b b 1_1357 1H 51.254 a a a a a a a a a a a 1_1498 1H 51.254 a a a a a a a a a a a 2_0514 1H 52.341 a - a b a a a a a b b 1_1266 1H 52.341 a a a a a a a a a a a 1_0294 1H 56.839 a a a b a a a a a b b 1_1209 1H 57.926 a a a a a a a a a a a 2_0810 1H 57.926 a a a b a a a a a b b 1_1107 1H 59.013 a a a a a a a a a a a 1_1217 1H 61.211 a a a a a a a a a a a 2_1217 1H 61.211 a a a b a a a a a b b 1_1256 1H 62.298 a a a a a a a a a a a 1_0768 1H 65.632 a a a b a a a a a b b 1_1444 1H 65.632 a a a a a a a a a a a 1_0201 1H 67.831 a a a a a b a a a b b 2_1463 1H 67.831 a a a a a a a a a a a 1_0617 1H 68.918 a a a a a a a a a a a 1_0302 1H 70.005 a a a a a a a a a a a 2_1431 1H 72.203 a a a b a b b a a b b 1_1367 1H 76.700 a a a a a a a a a a a 2_0696 1H 78.874 a a a a a a a a a a a 1_0166 1H 78.874 a a a a a a b a a b b 1_1075 1H 79.961 a a a a a a a a a a a 1_1267 1H 81.048 a a a a a a a a a a a 2_0121 1H 83.246 a a a a a a a a a a a 1_0279 1H 83.246 a a a a a a - a a b b 1_0466 1H 90.021 a a a a a a - a a b b

OWB

Chr. 1H

Bar

ones

se

BIS

ON

1H

BIS

ON

1H+

4H

BIS

ON

1H+

4H+

5H

BIS

ON

1H+

5H

BIS

ON

4H

BIS

ON

4H+

5H

BIS

ON

5H

BIS

ON

7H

BC

D12

BC

D47

2_0749 a b b b b a a a a b a2_0749

Unmapped in OWB

Ba

ron

ess

e

BIS

ON

1H

BIS

ON

1H

+4

H

BIS

ON

1H

+4

H+

5H

BIS

ON

1H

+5

H

BIS

ON

4H

BIS

ON

4H

+5

H

BIS

ON

5H

BIS

ON

7H

BC

D1

2

BC

D4

7

2_0749 a b b b b a a a a b a2_0749

Unmapped in OWB1_1223 0.02_1067 1.11_0410 3.32_0865 4.41_1011 5.51_0716 7.72_1226 8.72_0665 10.92_0318 17.92_0749 20.02_0371 21.12_0712 25.62_0767 27.81_0760 36.02_1072 37.11_0764 45.22_0514 46.32_1134 47.41_1301 51.91_1287 53.02_1357 55.21_0075 56.32_0609 57.41_02932_12172_1312

58.5

1_0324 60.61_0198 61.71_1248 62.81_07682_1333 63.9

1_0201 65.01_06171_1049 66.1

2_1306 68.32_1431 69.41_00021_1367 70.5

1_01661_0463 73.8

2_0229 77.12_1126 78.2

bPb-6318 0.0bPb-7112 1.1

1_0460 6.81_0410 7.91_0636 8.91_1011 11.1

bPb-1318 12.22_1226 14.4

[222208] 17.8[222789] 18.9

MWG837B 22.2scind00046 25.5

[223986] 26.6Bmac0399 28.8GBM1007 29.9

2_0712 33.2BCD098 35.4

bPb-3217 42.31_0760 46.8

GBM1042 47.91_0238 51.32_0514 52.31_0294 56.81_12871_13012_13572_1312

57.9

1_1107 59.02_1217 61.21_02931_0198 62.3

2_13331_0768 65.6

1_0201 67.81_06171_1049 68.9

2_1131 70.02_1431 72.21_13671_0002 76.7

ABC160 77.81_01661_0463 78.9

Morex x Barke OWB

2_0749

P-O

PA

1 +

2 m

ap

ch cM BA

RO

NE

SS

E

BIS

ON

1H

BIS

ON

4H

BIS

ON

1H+

4H

BIS

ON

5H

BIS

ON

1H+

5H

BIS

ON

4H+

5H

BIS

ON

1H+

4H+

5H

BIS

ON

7H

BC

D12

BC

D47

2_0275 1H 1.087 a b a b a a a b a b a1_1311 1H 6.775 a a a a a a a a a a a1_0410 1H 7.862 a a a a a a a a a a a1_0636 1H 8.949 a a a a - a a a a - -1_1011 1H 11.147 a a a a a a a a a a a

Act8 1H 12.2342_1226 1H 14.432 a b a b a a a b a b a

MWG837 1H 22.1882_0749 1H a b a b a b a b a b a2_0712 1H 33.230 a b a b a a a b a b b

BCD098 1H 35.4281_0760 1H 46.833 a a a b a a a a a b b1_0814 1H 47.920 a a a b a a a a a b b1_1357 1H 51.254 a a a a a a a a a a a1_1498 1H 51.254 a a a a a a a a a a a2_0514 1H 52.341 a - a b a a a a a b b1_1266 1H 52.341 a a a a a a a a a a a1_0294 1H 56.839 a a a b a a a a a b b1_1209 1H 57.926 a a a a a a a a a a a2_0810 1H 57.926 a a a b a a a a a b b1_1107 1H 59.013 a a a a a a a a a a a1_1217 1H 61.211 a a a a a a a a a a a2_1217 1H 61.211 a a a b a a a a a b b1_1256 1H 62.298 a a a a a a a a a a a1_0768 1H 65.632 a a a b a a a a a b b1_1444 1H 65.632 a a a a a a a a a a a1_0201 1H 67.831 a a a a a b a a a b b2_1463 1H 67.831 a a a a a a a a a a a1_0617 1H 68.918 a a a a a a a a a a a1_0302 1H 70.005 a a a a a a a a a a a2_1431 1H 72.203 a a a b a b b a a b b1_1367 1H 76.700 a a a a a a a a a a a2_0696 1H 78.874 a a a a a a a a a a a1_0166 1H 78.874 a a a a a a b a a b b

0 5 10 15 20 25

RGA54

act8

MWG8372_0749*

BMAC213

BCD098

BMAC222

E33M42h

BMAC90

ABC160

BMAC303

BMAC144

HvHVA1

Shyri x Galena

2_0749*

2_0749* Inferred position

2_0749*MWG837

BCD098

Act8

OWB

Chrom. 1H

P-O

PA

1 +

2 m

ap

ch cM BA

RO

NE

SS

E

BIS

ON

1H

BIS

ON

4H

BIS

ON

1H+

4H

BIS

ON

5H

BIS

ON

1H+

5H

BIS

ON

4H+

5H

BIS

ON

1H+

4H+

5H

BIS

ON

7H

BC

D12

BC

D47

1_1260 5H 59.928 a a a a b b b a a b b2_0396 5H 63.262 a a a a b b b a a b b2_0059 5H 63.262 a a a a a a a a a a a2_0105 5H 65.460 a a a a a a a a a b a2_1536 5H 65.460 a a a a b b b a a b b1_1151 5H 66.547 a a a a a a a a a a a1_0237 5H 66.547 a a a a b b b b a b b1_1523 5H 67.634 a a a a a a a a a a a2_1036 5H 70.895 a a a a a a a a a a a

Ale 5H 70.8951_0641 5H 74.230 a a a a a a a a a a a1_1249 5H 74.230 a a a a b b b a a b b1_0548 5H 81.137 a a a a a a a a a a a2_0297 5H 82.224 a a a a a a a a a a a1_0953 5H 83.311 a a a a a a a a a a a2_0818 5H 86.646 a a a a a a a a a a a2_1133 5H 92.179 a a a a a a a a a a a2_0246 5H 95.513 a a a a a b a a a b b1_0140 5H 95.513 a a a a a a a a a a a1_1515 5H 96.600 a a a a a a a a a a a2_1186 5H 99.885 a a a a a a a a a a a1_1135 5H 103.220 a a a a a b a b a b b1_0674 5H 107.717 a a a a a a a a a a a1_0524 5H 111.052 a a a a a b a a a b b1_0930 5H 112.139 a a a a a a a a a a a1_0578 5H 113.226 a a a a a a a a a a a1_0771 5H 114.313 a a a a a a a a a a a2_0449 5H 114.313 a a b a a b a a a b b

srh 114.3131_0518 5H 114.313 a a a a a b a a a b b1_1350 5H 122.307 a a b a a b a a a b b2_0403 5H 123.394 a a a a a a a a a a a2_0134 5H 124.481 a a a a a a a a a a a1_0024 5H 126.680 a a a a a a a a a a a1_0844 5H 127.767 a a a a a a a a a a a1_0507 5H 129.965 a a a a a a a a a a a1_0477 5H 132.163 a a b a a a a a a b b1_1341 5H 133.244 a a a a a a a a a a a1_1245 5H 141.402 a a a a a a a a a a a

0 2 4 6 8

10 12 14ABC164b

PR168a

ABG387c

HVM2A

Ale

HVLEU

CDO57

ABC168

WG364b

0 2 4 6 8

10 12 14

ABC164b

PR168a

ABG387c

HVM2A

Ale

HVLEU

CDO57

ABC168

WG364b

OWB Chrom. 5H

Cali sib x Bowman

BCD14340DsT-667Act8A12RbgMD18MWG837B22scind0004625ABC165C26Bmac039929GBM100730BCD09836GBM104248BG36701354Bmag021158BG36994061GBM105168ABC16073JS10C86Bmac0144A87MWG706A96KFP170101Blp111ABC261119MWG2028121KFP257B122WMC1E8130MWG912133ABG387Ascssr04163scssr08238

136

1H

DsT-10ABG0585scind026227

ABG00817

scssr1022636scssr0775939GBM106642Pox45scssr0338156scssr12344scssr02236Ebmac0684

63

BCD1434.265ABG35668GBM102371scsnp0334383vrs188Bmag012594DsT-4197MWG503102GBM1062103KFP203104MWG882A108ABG1032117ABG072124Ebmc0415137cnx1139Zeo1149GBM1019161Aglu5F3R2163MWG720165GBM1012170wst7173scssr08447179MWG949A180

2H

BCD9070

ABC171A26GBM107430scssr1055933MWG798B36Dst-2739BCD70642DsT-3958alm61Bmac020966ABC32569DsT-6773

scssr2569187ABG37789Bmag022598

Act8C121ABG499124GBM1043125

scsnp23255151ABG004155

scind02281166MWG883172

DsT-24181

HVM62190

DsT-40199

ABC172scssr25538212

DsT-35218

3H

MWG6340MWG07721HVM4024DsT-2929CDO54230CDO12231hvknox335Dhn639ABC303scssr2056941

CDO79544HVM349DST-46scind03751scssr18005

50

Tef252GBM102060Bmag035362scind1045567DsT-7974scssr14079ABG47280

GBM105983KFP22192Ebmac070194MWG652B95GBM1048101Hsh111HVM67112KFP241.1116ABG601124

4H

scssr023060MWG6186DsT-68ABC48311ABG61012

ABG39537scssr0250344scssr1807645Bmac009653NRG045A55scsnp0426056Ale58

ABC30279scind1699182scssr1533485scsnp0614490srh100

scssr05939111

RSB001A120

scsnp001771280SU-STS1134ABG003B141

scssr10148157Tef3166MWG877169BE456118A170ABG496179scsnp02109E10757A193

ABG391197JS10B198ABC622205DsT-33207Bmag0113C215MWG602A223scssr03907224scssr03906225

5H

MWG6200Bmac0316scssr093984

MWG652A31MWG602B35scind6000242JS10A45GBM102151GBM106861BG29929765HVM3168rob70Bmag0009scssr0209371

ABG47481Bmac0218C88ABG38892scsnp2122699MWG820101GBM1008122scssr05599123MWG934126scind04312b132scssr00103GBM1022135

Bmac0040143DsT-18145DsT-32B146DsT-22152DsT-28159scind60001DsT-74160

MWG514162MWG798A163DsT-71167

6H

ABG7040Bmag000714scind0069420AW98258029MWG089CDO47536

ABG38038BE60207344scssr0797057scsnp0046066ABC25568ABC165D69HvVRT273scssr1586482GBM103086scsnp22290MWG808DAK642scind00149

89

scsnp00703MWG203197

RSB001C98nud103lks2115ABC1024117Bmag0120125DsT-30126WG380B127ABC310B137Ris44139

ABG461A167WG380A171GBM1065178

HVM5196scssr04056KFP255197

ThA1199

7H

Flanking markers in each target region

Three resistance genes in chromosome 1H, 4H and 5H

THE PROBLEM

KURTFORD

•6 –rowed hooded feed barley

•Well adapted to California conditions

•Short height

•Stripe Rust Susceptible

iBISON 1H+4H+5H

• Resistance alleles for the three genes

• 2–rowed awned barley; short height

I Bison

1H 4H 5H

Kurtford

1H 4H 5H

XF1 heterozygote at 3 BSR resistance loci

X

Kurtford

1H 4H 5H

BC1F1 12.5% of plants expected to be heterozygote at 3

BSR resistance loci.

Year 1

RR RR RR SS SS SS

RS RS RS SS SS SS

Segregation in each locus:

½ RS½ SS

SOLUTION: The Kurtford Conversion

We have 589 BC1F1 seeds and we expect to find around 74 target heterozygotes at the 3 BSR resistance loci.We need:

-Confirm choice of markers-DNA extraction for 589 plants-Genotyping for the 3 BSR resistance regions-Selection of targeted genotypes

Bmag399

EST4473

EST4535

Baal29j18

. Bags 4e

Bmag337

Bmag223

Bmag812

1H 4H 5H

Summer – Fall 2007

MAS1

Kurtford iBison 1H+4H+5H

I I I K I K I I K

ES

T4

47

3,

1F

/4R

, B

an

II

Bm

ag

39

9

ES

T4

53

5,

1F

/5R

, N

arI

Ba

al2

9j1

8

Ba

gs

4e

Bm

ag

22

3

Bm

ag

81

2

BM

AG

33

7

Pla

nt N

um

ber

1H

1H

4H

4H

4H

5H

5H

5H

1 I I K K K I I K2 K K K K K I I K3 K K I I I K K K4 I I K K K K K I5 I I I I I I I I6 I I I I I K K I7 K K K K K I K K8 K K I I I I I I9 K K K K K I I K

10 I K K K K I I K11 K K I I I I I I12 I I K K K I K I13 I I I I I I I K14 I I I I I I I I15 I I I I I K K K16 K K K K K I I K17 K K I I I K K K18 K K K K K I I19 I I I I I I I I20 I K I I I I I K

1. Selection of heterozygotes (~74) 2. 10 seeds per selected plant ~ 740 BC1F2 plants.3. Grow out and genotype

We expect 25% of the BC1F2 to be homozygous for AT LEAST the 4H region (~ 185). Of these:

~ 104 homozygote only for 4H~ 35 homozygote for 4H and 1H~ 35 homozygote for 4H and 5H~ 11 homozygote for 1H, 4H and 5H

25/64 (~ 38%) of the resistant plants will be homozygous hooded

Winter 2008

MAS2BC1F2 plants segregating in each locus:¼ RR ½ RS ¼ SS

Winter 2008/2009 – seed increase

Summer 2009 – validate resistance

~ 70 homozygous hooded, BSR resistant plantsField test

Abundant, gene-based markers make MAS more effectiveand informative

What makes a trait quantitative and not qualitative? Successful MAS may precede gene characterization Phenotypic performance is the ultimate measure of agronomic worth

MAS - coming to a field near you