Mark Allan, Trans Ova Gene0cs June 19, 2019 · 2019. 7. 1. · Mark Allan, Trans Ova Gene0cs June...
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Mark Allan, Trans Ova Gene0cs June 19, 2019 2019 BIF Symposium, Brookings, S.D. 1 New Technologies in Cattle Reproduction and the Correlated Acceleration of Genetic Gain Mark Allan, PhD Director of Gene0c Technology Trans Ova Gene0cs-ProGentus 1 Genetic Selection – Animal Breeding • Been going on for thousands of years • Has accelerated drama5cally last 100 yrs • Massive progress in the last 50 yrs • Extremely organized systems of gene5c improvement in the livestock sectors 2 Beef- Genetic Improvement 3 Year lbs beef per female #s 1965 375 lbs 1975 398 lbs 1985 508 lbs 1995 523 lbs 2007 618 lbs USDA –Na0onal Agriculture Sta0s0cs Service Calculated: Annual Total lbs Beef Produced/Total Numbers of Females • 1975 23.1 billion lbs beef with female counts of 58 million. • 2007 26.3 billion lbs beef with female counts of 42.5 million Dairy 1940s • 25 M cows 4500 lbs per lacta5on 2008 • 9.2 M cows 22,000 lbs per lacta5on !2.7 fold reduc5on in cow numbers !4.9 fold milk yield per cow increase lb CO 2 per lb Milk 1940 cows required 4.5 5mes more land and produced 2.6 5mes more Methane. 4 Selection Information – Genetic Tool box • Visual appraisal • Pedigree informa0on • Pedigree verifica0on • Performance data • Gene0c Evalua0on and accuracy • Economic indexes • Gene0c tests for simple recessives • Targeted panels • High density panels • Genomic enhanced EPDs • Imputa0on • Whole genome sequence 5 The ART Toolbox – Compliments Genomic/Genetics Embryo Transfer In Vitro Fer0liza0on Cloning Technology Precision Breeding Gene Edi0ng Sorted Semen Reproduc5ve Toolbox Recipient Solu0ons Gene0c Resources Ar0ficial Insemina0on Animal Husbandry 6
Transcript of Mark Allan, Trans Ova Gene0cs June 19, 2019 · 2019. 7. 1. · Mark Allan, Trans Ova Gene0cs June...
MarkAllan,TransOvaGene0cs June19,2019
2019BIFSymposium,Brookings,S.D. 1
New Technologies in Cattle Reproduction and the
Correlated Acceleration of Genetic Gain
MarkAllan,PhD
DirectorofGene0cTechnology
TransOvaGene0cs-ProGentus
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Genetic Selection – Animal Breeding • Beengoingonforthousandsofyears• Hasaccelerateddrama5callylast100yrs• Massiveprogressinthelast50yrs• Extremelyorganizedsystemsofgene5cimprovementinthelivestocksectors
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Beef- Genetic Improvement
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Year lbsbeefperfemale#s1965 375lbs
1975398lbs1985 508lbs1995 523lbs2007 618lbs
USDA–Na0onalAgricultureSta0s0csService
Calculated:AnnualTotallbsBeefProduced/TotalNumbersofFemales
• 197523.1billionlbsbeefwithfemalecountsof58million.
• 200726.3billionlbsbeefwithfemalecountsof42.5million
Dairy
1940s• 25Mcows4500lbsperlacta5on 2008• 9.2Mcows22,000lbsperlacta5on
! 2.7foldreduc5onincownumbers! 4.9foldmilkyieldpercowincrease
lbCO2perlbMilk
1940cowsrequired4.55mesmorelandandproduced2.65mesmoreMethane.4
Selection Information – Genetic Tool box • Visualappraisal• Pedigreeinforma0on• Pedigreeverifica0on• Performancedata• Gene0cEvalua0onandaccuracy• Economicindexes• Gene0ctestsforsimplerecessives• Targetedpanels• Highdensitypanels• GenomicenhancedEPDs• Imputa0on• Wholegenomesequence
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The ART Toolbox – Compliments Genomic/Genetics
EmbryoTransfer
InVitroFer0liza0on
CloningTechnology
PrecisionBreedingGeneEdi0ng
SortedSemen
Reproduc5veToolbox
RecipientSolu0onsGene0cResources
Ar0ficialInsemina0on
AnimalHusbandry6
MarkAllan,TransOvaGene0cs June19,2019
2019BIFSymposium,Brookings,S.D. 2
Why use ART?
Assistedreproduc0ontoolscoupledwithgenomicenhancedselec0onwillaccelerate:
gene5cgain
AIstartedin1950’sETstartedin1970’s IVFstartedin1990’sGP/Cloningstartedinlate1990’sSSstartedin2000’s
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Genetic Improvement –
Genetic Gain and Genetic Specifications
Gene$cGain=accuracyxgene$cvaria$onxintensity
genera$oninterval
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Embryo Transfer – in vivo, in vitro
ET allows you to increase the genetic impact of superior females.
More than one calf per year out of those really good cows.
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Embryo Production by IVF TOG Early 90’s
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The IVF Process
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MarkAllan,TransOvaGene0cs June19,2019
2019BIFSymposium,Brookings,S.D. 3
IVF and genomics
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Genetic Gain- Dairy the Race to win
Gene0cGain=accxgene0cvaria0onxintensity
genera5oninterval
• “Race”–gene0cimprovementYoungAnimals
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1)Qualifydonorsviahighdensitygenomicchips-GPTAs
2)Collectjuveniledonors
4)Gestateallembryos-Genera0oninterval
3)IVFwithsexed/conven0onalsemen
Today’s Elite Genetic Selection USDA/University/IlluminaConsor0umBovine
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Progenyequivalents=Accuracy/Reliability
Genomic Enhanced Results Dairy
Largecommercialdatasetslineupwellwithagene0ctool!!!!! 16
GenomicsImpactontheDairyIndustry
0
10000
20000
30000
40000
50000
60000
1 5 9 13 17 21 25 29 33 37 41 45 49 53 57 61 65 69 73 77 81 85 89 93 97101105109113
HOGenomiccountsmonthly2009to4/2019
Date NumberHOMonth
TotalAnimalssubmijedtoCDCBApril2019
3,124,849
2009-2010- 2011- 2012- 2013- 2014- 2015- 2016- 2017-2018-2019-
Tes0ngcost$$30-$50/animal
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Keys to the Success of HO and JE Genetic Evaluations
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• Data• Progenytes0ngwithcommercialdrivendatasets• Phenotypes-dairyindustryhasexcellentrecords
• Repeatedrecordsperfemaleperlacta0on• Largeamountsofunbiaseddata-clean• Connec0vityNorthAmericaandaboard
• AIgreater95%USdairypopula0on
MarkAllan,TransOvaGene0cs June19,2019
2019BIFSymposium,Brookings,S.D. 4
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History Parent Averages and Full-sibs X
PA
(Reference: Zoetis Clarified) 20
Restructuring of the Industry
Value Distribution of Genetics
Genetics Industry – 10 Years Ago - Dairy
Select
ABS
Cogent
aa
ds
Genex
Alta
Semex
Accelerated
SeedstockProducers Gene5cCompanies
CommercialOpera5ons
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TODAY’s Genetics Industry – Dairy
Seedstockproducers
Select
Accelerated
ABSIVB
ds
sv
SemexBoviteq
STST
TAG
GS
Cogent
Alta
GenexMinitube
peak
CommercialOpera5ons
Gene5cCompanies
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Outcomes Dairy • Extremevaluedifferen0a0onofyounganimals.
• Consolida0on-gene0csandcommercialproduc0on• Con0nueddevelopmentofnucleuspopula0ons
• Proprietarygene0cs• Enduseragreements-programs
• Priva0zinggene0cevalua0onsystems
• Ver0calintegra0onlikeporkandpoultry?Concep0ontoConsump0on
• AcceleratorofDairy/Beefprograms
Acceleratedbytechnology(ART&Genomics)
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Where’s the Beef
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MarkAllan,TransOvaGene0cs June19,2019
2019BIFSymposium,Brookings,S.D. 5
Progenyequivalents=accuracy/Reliability
Beef
Genomic enhanced results
(AmericanAngus2019)
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Genetics industry –today - Beef SeedstockProducers
Gene5cCompanies
CommercialOpera5ons
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Future- Beef • Genera0oninterval-youngeranimals?• Gene0clandscapechanging?• Leveragingdatalikethedairyindustry?
• Largerprogenytestswithhighqualitydata• Impactoftechnologyincommercialsectorincreases?• ChangeandImprovement
AmericanHereford2009
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Gene5cSelec5on–FutureGenera5onintervalreduced
Fetalcelllineselec5onEmbryocelllineselec5onBiopsy–freezeembryosYoungfemaleoocyterecoveryL-OPU
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Fetal cell line selection
& Embryo cell line
selection
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L-OPU
• Collec0onoocytesDonor40daysofage.• CalfgPTAsforselec0on
• DonorDam11monthsold
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• Produc0oninnumbers• Averagedonorageegg
produc0on60dayto150daysofage
MarkAllan,TransOvaGene0cs June19,2019
2019BIFSymposium,Brookings,S.D. 6
Steak & Hamburger with a story
Traceability, food safety
FoodDistribu5on
HowdoestheBeefgene0cpyramidchangeover0me?
S0ll#1,collec0onofqualityphenotypicdataforinputandoutputtraits. 31
QUESTIONS?www.transova.com
THANKYOU
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