Magnetic Resonance Imagingfor Pre-clinical Applicationspplications

2

Applications

Brain Diffusion Tensor Imaging at 9.4T

Data courtesy: Laboratory of Functional and Metabolic Imaging,

Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

k-t SENSE Cardiac Imaging at 9.4T

Data Courtesy: CABI, University College London,

and BIC Imperial College London, UK

Sodium MRI of Kidney at 9.4T

Data Courtesy: Indiana University School of Medicine

Agilent’s range of MRI systems are used in a variety of

applications. Each system is carefully confi gured to meet

your requirements and your demands, while offering the best

performance of that system.

Some of the pre-clinical applications include:

• Brain and organ imaging

• Cardiac investigation

• Tumour assessment

• Investigation of contrast agents

• Magnetic resonance spectroscopy

We understand that data and image acquisition can be time

consuming and labor intensive. Therefore, our systems are

designed to improve throughput, increase effi ciency and

improve accuracy, allowing you to collect high quality data.

9.4T (above) and 14.1T (right) Brain Spectroscopy

Data courtesy: Laboratory of Functional and Metabolic

Imaging, Ecole Polytechnique Fédérale de Lausanne,

Lausanne, Switzerland.

3

Complete, Flexible MRI Systems

Agilent offers a complete range of pre-clinical MRI systems.

Each Agilent MRI system includes the DD2 console, a

high fi eld or ultra-high fi eld magnet, gradient and RF coils,

VnmrJ 3.1 software, and a selection of sample handling

options to meet your specifi c needs.

Working together with a range of clients, Agilent is able to

produce high fi eld magnetic systems exhibiting very high

stability and consistency. The architecture of the Agilent DD2

console allows superior performance on multiple channels.

Our VnmrJ 3.1 Software supports an extensive library of 2D,

3D and advanced MRI pulse sequences.

Data courtesy: Center for Magnetic Resonance Research, University of Minnesota,

Minneapolis, MN, USA.

4

High Field MRS4.7-9.4T Horizontal Bore Magnet Systems

9.4T/310mm

7T/210mm

Specifi cations for Horizontal Bore Magnet

Product

MRBR MRBR MRBR MRBR MRBR MRBR MRBR MRBR MRBR

4.7T/310 7.0T/160 7.0T/210 7.0T/310 7.0T/400 9.4T/160 9.4T/210 9.4T/310 9.4T/400

ASR ASR ASR ASR ASR ASR ASR ASR ASR

Operating fi eld (T) 4.7 7 7 7 7 9.4 9.4 9.4 9.4

Bore Size excl RT shim and Gradients (mm)

310 160 210 310 400 160 210 310 400

Homogeneity volume (mm DSV) 150 80 80 140 200 80 80 140 200

Homogeneity: fully shimmed peak to peak (ppm) / (mm) DSV

<±5ppm / 150mm

±2ppm / 80mm

±2ppm / 80mm

±2.5ppm / 150mm

±2.5ppm / 200mm

±2ppm / 80mm

±2ppm / 80mm

±2.5ppm / 140mm

±2.5ppm / 200mm

Homogeneity: superconducting only peak to peak (ppm) / mm DSV

±5ppm / 80mm

±4ppm / 80mm

±10ppm / 150mm

±10ppm / 200mm

±4ppm / 80mm

±4ppm / 80mm

±10ppm / 140mm

<10ppm / 200mm

System length (mm) 1280 1012 1280 1636 1998 1224 1420 1704 2286

Minimum ceiling height (mm) 3150 3130 2485 3030 3033 3300 3030 2990 3530

System diameter (mm) 1360 1350 1250 1655 2171 1500 1655 1740 2708

Zero boil-off Yes Yes Yes Yes Yes Yes Yes Yes Yes

Fringe fi eld (5 Gauss)(Axial x Radial) (m)

2.3 x 1.5 2.1 x 1.1 1.5 x 1.4 2.6 x 1.2 4.2 x 2.3 2.4 x 2.0 3 x 2 3.6 x 2.2 5 x 3.6

Pre-Clinical MRI at its Best

High Field MRI Systems

Available in a range of bore sizes from 160-900mm and in

fi eld strengths from 4.7-9.4T, Agilent's high fi eld magnets

are renowned for their market leading performance. Most

systems are available with active shielding technology. The

proton operating frequency of each system is dependent upon

fi eld strength.

The core products in the range comprise two MRI systems.

The Agilent MRI System is an adaptable MR imaging platform

that can be utilized in many MRI applications well as in the

development of novel research processes.

The Discovery MR901 System is a complete pre-clinical MRI

system operating on a clinical environment interface facili-

tated by GE Healthcare.

5

Ultra High Field MRI11.7-18.8T Horizontal Bore Magnet Systems

11.74T/160mm

16.4T/260mm

Meeting our Customers’ Needs

Agilent’s Ultra High Field MRI magnets adopt the same high

standards and practices as the high fi eld magnets. In fi eld

strengths of 11.7T to 18.8T, and with bore sizes 160-400mm,

these cutting-edge designs take into account every aspect

of a client’s needs, including ease of use, running cost and

space constraints.

We incorporate market-leading superconducting technology

to meet even the most demanding requirements and techno-

logical specifi cations.

And with each one being built for individual purposes and

customer requirements, you can be confi dent of superb MRI

performance every time.

Specifi cations for UHF Horizontal Bore Magnet Systems

Product

MRBR MRBR MRBR MRBR MRBR MRBR MRBR MRBR

11.7/160 11.7/210 11.7/310 11.7/400 14.1/260 16.4/260 17.6/210 18.8/210

Active Active Passive Passive Passive Passive Passive Passive

Operating fi eld (T) 11.7 11.7 11.7 11.7 14.1 16.4 17.6 18.8

Operating temperature (K) 4.2 2.3 2.3 2.3 2.3 2.3 2.3 2.3

Bore Size excl RT shim and Gradients (mm) 160 210 310 400 260 260 210 210

Homogeneity volume (mm DSV) 80 100 150 200 130 100 100 100

Homogeneity fully shimmedpeak to peak (ppm /mm DSV)

±2.5ppm / 80mm

±2.5ppm / 100mm

±2.5ppm / 150mm

±2.5ppm / 200mm

±2.5ppm / 130mm

±4ppm / 100mm

±2.5ppm /100mm

±2.5ppm /100mm

Homogeneity super conductingonly peak to peak (ppm /mm DSV)

±10ppm / 80mm

±10ppm / 100mm

±5ppm /100mm

±5ppm /100mm

±10ppm /130mm

±10ppm / 100mm

±5ppm /100mm

±5ppm /100mm

Minimum Hold Time between Helium refi lls (days) 365 365 50 50 50 50 32 32

Minimum Hold Time between Nitrogen refi lls (days) N/A N/A 8 8 14 14 14 14

System length (mm) 1400 1680 2240 2600 2132 2572 2572 2920

Minimum ceiling height (mm) 2950 3030

System diameter (mm) 1840 1690 2100 2380 1820 2100 2100 2380

In vivo brain phase imaging at 14.1T

Data courtesy: Laboratory of Functional and Metabolic Imaging,

Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

6

Gradient CoilsHigh Duty Cycle Gradient Coils

Nested Gradients

Agilent 205/120 HD gradient,

including cutaway 3D CAD

Agilent’s latest generation of high performance gradients has

been designed and developed by MR scientists to address the

most challenging techniques and applications at the highest

magnetic fi elds.

Features include:

• Excellent heat extraction, providing industry-leading

high duty cycle performance

• Improved peak strength with short rise times

• Microgroove technology for superior magnetic shielding

• High slew-rates

• Superior gradient linearity

• High strength room-temperature shims

• HD 305/210 and HD 205/210 now rated to 300A peak

current providing increased gradient strength performance

Specifi cations for Gradients

Outside diameter: 395mm 305mm 205mm 156mm 156mm 115mm

Inside diameter: 290mm 210mm 120mm 100mm 90mm 60mm

Peak Current: 300A 300A 300A 200A 200A 200A

Peak Voltage: 500V 500V 500V 300V 300V 300V

Gradient sensitivity: 0.333mT/m/A 1.0mT/m/A 2.0mT/m/A 2mT/m/A 3.75mT/m/A 5.0mT/m/A

Maximum gradient strength: 100mT/m 300mT/m 600mT/m* 400mT/m 750mT/m* 1000mT/m*

Maximum slew rate: >380T/m/s >700T/m/s >4444T/m/s >3000T/m/s >5770T/m/s >7690T/m/s

Maximum DC current in all 3 axes simultaneously, I

DC Max

100A 75A 75A 55A 45A 50A

Duty cycle @ IMax

: 11.1% 6.25% 6.25% 7.6% 5.1% 6.25%

Minimum inductive rise time: 162μsec 229μsec 38μsec 50μsec 54μsec 16μsec

Gradient sub-system rise time: 260μsec 425μsec 135μsec 130μsec 130μsec 130μsec

Linearity (% over DSV): <5 %/200mm <5 %/120mm <5 %/80mm <5 %/75mm <5 %/60mm <5 %/40mm

Number of shims: 9 (including gradient shims)

Shim strengths:

Z0 348mG/A 380mG/A 530mG/A 470mG/A 505mG/A 510mG/A

Z2 16.6mG/cm2/A 29.7mG/cm2/A 87mG/cm2/A 89.7mG/cm2/A 127mG/cm2/A 157mG/cm2/A

ZX, ZY 5.1mG/cm2/A 12.2mG/cm2/A 41mG/cm2/A 62mG/cm2/A 73.5mG/cm2/A 124mG/cm2/A

2XY, X2-Y2 2.5mG/cm2/A 5.6mG/cm2/A 12.7mG/cm2/A 16.8mG/cm2/A 23.5mG/cm2/A 40mG/cm2/A

Shim algorithm 3D Automatic

Shim algorithm Manual Interactive

7

A key feature of Agilent’s complete range of RF coils is the

high level of RF homogeneity and stability, which is vital to

effective imaging, whether in the transmit or receive phase,

while maintaining excellent signal to noise ratios.

We have an extensive catalogue of RF coils, which fall into

fi ve key categories central to MRI applications. These are:

• Millipede: Suitable for whole body scanning and micro-

imaging, these coils produce consistent imaging with

reduced potential systematic errors

RF Coils

• Volume: suitable for all pre-clinical applications, the sample

fi ts fully inside the coil, minimizing the distance between

the coil body and the sample surface.

• Surface: ideal for oncology, surface coils enable increased

signal-to-noise ratios.

• Phased Array: ideal for neurological, spinal and cardiac

imaging, these coils are available in a variety of anatomical

spatial arrangements.

• Dual-tuned: these can increase your productivity by a signif-

icant amount, because they capture data at two different

frequencies at the same time. Volume and surface coils are

available in dual-tuned format.

For Every Application

Selection of Phased Array RF Coils and support devices.

Millipede RF Coil

8

Sample HandlingSample Positioning System

Cart Option

SPS 60 for use with the

60mm inner diameter

gradient coil SPS 100

LITE version of

sample handling for

small lab settings

Table Top Sample

Handling System

Detail of Table Top Option

Cradle OD

Nominal Sample weight

RF coil Positioner confi guration Magnet bore size for 4.7T – 11.7T

mm Up to gmVolume/

phased arrayMillipede Brain Cardiac Surface LITE Table Cart

RF shielding Option

160mm 210mm 310mm 400mm

29 25 ● ● ● ● ● ● ●

33 25 ● ● ● ● ● ● ●

38/39 30 ● ● ● ● ● ● ● ●

62 30 ● ● ● ● ● ● ● ● ● ●

71 300 ● ● ● ● ● ● ● ● ● ● ● ●

138 300 ● ● ● ● ● ● ● ● ●

148 300 ● ● ● ● ● ● ● ● ●

Designed for Ease-of-use

Agilent’s sample handling products are able to meet customer

demands, whatever the specifi cations. Each is made to a very

high standard, and greatly improve ease of sample handling

and image quality.

We offer two options: the LITE system for unscreened rooms,

and either the table top or cart system for screened rooms. .

Features and Benefi ts

• Fine adjustment from the end of the positioner ensures

repeatable placement of your sample in the iso-centre.

• Positioners and sample cradles are available across our

entire range of gradients.

• Easy adjustment of the table height seamlessly accomo-

dates sample preparation and insertion.

• The large fl at surfaces of the table and cart allow conven-

ient access to additional equipment.

9

SoftwarePulse Sequence Library

FSEMS protocol

GEMS protocol

Agilent Pulse Sequence Library

The Agilent MRI sequence library is constantly being revised,

updated, and enhanced. The sequences are fully parameter-

ized for maximum fl exibility.

• VnmrJ 3.1 Software

• Standard 2D Imaging Sequences

• Diffusion-weighted 2D Imaging Sequences

• Standard 3D Sequences

• Advanced MRI Sequences

• Magnetic Resonance Spectroscopy (MRS) Sequences

• Shimming

Standard 2D Imaging Sequences

All standard 2D sequences described below are designed for

ease of use.

• SEMS – A 2D spin-echo MRI

• MEMS – A 2D multi-echo MRI

• GEMS – A 2D gradient-echo MRI

• MGEMS – A 2D gradient-echo MRI with multi-echo

acquisition

• GEMSIR – An inversion recovery MRI with 2D gradient-

echo - can be used for T1map

• FSEMS - A 2D Fast Spin Echo MRI

• FLAIR – Fluid-attenuated inversion recovery MRI

• Echo Planar 2D Imaging Sequence - Designed for use by a

non-MRI expert using a simple setup pre-scan for routine

EPI imaging

Advanced MRI Sequences

• Cardiac MRI – for looking at phases of the cardiac cycle

and reconstruction of the images for creating CINE views of

the beating heart

• Arterial spin labeling MRI – for measuring perfusion

• EPI-FAIR – slice-selective inversion recovery pulse on the

imaging slice. The control is a non-selective inversion

recovery pulse.

• EPI-STAR – slab-selective inversion recovery pulse applied

below the imaging slice. The control is above the imaging

slice.

• EPI-PICORE – slice-selective inversion recovery pulse

applied below the imaging slice. The control is a non-

selective inversion recovery pulse.

• SSFP – A steady-state free precession MRI

• Localized Spectroscopy - LASER, SPECIAL, Short Echo

STEAM

10

Site Planning

Pre-clinical 9.4T/160

imaging suite with

preparation area

Pre-clinical 7T/310

imaging suite with

separate preparation room

Your Partner in Planning

Evaluating and deciding on the right MRI system takes time,

but it is just the beginning of our relationship with you the

customer.

Here at Agilent, we know that our role doesn’t stop when

your system is ready. Site planning, and ensuring the correct

pre-requisites are in place, are just as important as helping

you select which system meets your research needs.

We will be on hand to help you plan and prepare the loca-

tion for the installation of your new imaging equipment. This

includes an additional site survey to identify and eliminate

potential issues that could impact operation of the magnet

once it is energized.

Maximum Capability, Minimum Footprint

All of our systems are designed to have the smallest

physical footprint possible, while providing you with

maximum imaging capability.

We recommend you have 3-4 rooms dedicated to your new

MRI system, but we will work with you to fi nd a solution to

whatever space constraints you may have.

Implementation Timeline

The implementation of our system is designed to be as effi -

cient as possible, allowing you to continue with your research

quickly.

The four key dates of the system implementation timeline are:

• Start of the project

• Build of the installation environment

• Delivery and installation of magnet system

• Commission and hand-over to the customer

Protecting Your Assets

RF shielding is an available option

with all of our systems, giving you

peace of mind that your installation

will provide a safe working environ-

ment for all personnel who come into

contact with the equipment.

The RF room is a custom, insulated,

turn-key magnet room, which includes

effective sound proofi ng designed to

shield the room’s exterior, while an

oxygen monitor gives you clear indica-

tion of the working conditions inside.

1111

Agilent is a name that is synonymous with measurement.

With expertise that spans electronic measurement, chemical

analysis and the life sciences, you can be sure that our prod-

ucts will meet your toughest requirements.

We pride ourselves on providing the world’s most complete,

most reliable laboratory productivity solutions, optimized for

your applications and workfl ows. Through a combination of

industry-leading instruments, accessible scientifi c expertise,

The Measure of Confi dence

Discovery MR901 (7T/310)

Agilent's magnet facility in Yarnton, UK.

9.4T/310mm magnet

easy-to-use software and a full range of global support

services, we are committed to delivering better results, faster

than ever.

Our MRI systems are no different. Our extensive expertise

dates back to the founding of Magnex Scientifi c in 1982. As

a result, our customers have benefi tted from the experience

and knowledge of our design teams and scientists, who bring

a wealth of information into the design of each and every

system we build.

www.agilent.com

Product specifi cations and descriptions in this document are subject to change without notice.

© Agilent Technologies, Inc., 2011Published in USA, July 01, 2011Publication Number 5990-8104EN

At a Glance

We have provided this table so that you can compare our

best selling systems at a glance. Further information can

be found inside this brochure, or by contacting us using

the information on this page.

For more information

Learn more:

www.agilent.com/chem

Find an Agilent customer center in your country:

www.agilent.com/chem/contactus

U.S. and Canada

1-800-227-9770

agilent_inquiries@agilent.com

Europe

info_agilent@agilent.com

Asia Pacifi c

adinquiry_aplsca@agilent.com

Contact the MRI team

mri.info@agilent.com

Magnet System 7T (300MHz) 9.4T (400MHz)

Bore size 210 310 210 310

Length 1280mm 1636mm 1420mm 1719mm

Width 1200mm 1690mm 1690mm 1740mm

Min. ceiling height 3125mm 3030mm 3030mm 2990mm

Homogeneity (ppm/DSV)

Fully shimmed < ±2 / 8cm

< ±2.5 / 15cm

< ±2 / 8cm

< ±2.5 / 14cm

Superconducting only < ±4 / 8cm

< ±10 / 15cm

< ±4 / 8cm

< ±10 / 14cm

Fringe Field (5 Gauss line)

Radial 1.5m 2.1m 2m 2.2m

Axial 1.4m 2.6m 3m 3.6m

Main Gradient

Outside diameter 205mm 305mm 205mm 305mm

Inside diameter 120mm 210mm 120mm 210mm

Peak current 300A 300A 300A 300A

Max. gradient strength 600mT/m 300mT/m 600mT/m 300mT/m

Sample Positioning (optional)

Trolley

OD of optional positioners available

N/A 210mm N/A 210mm

120mm 120mm 120mm 120mm

60mm 60mm 60mm 60mm

Table

OD of optional positioners available

N/A 210mm N/A 210mm

120mm 120mm 120mm 120mm

60mm 60mm 60mm 60mm

LITE

OD of optional positioners available

N/A 210mm N/A 210mm

120mm 120mm 120mm 120mm

100mm 100mm 100mm 100mm

90mm 90mm 90mm 90mm

60mm* 60mm* 60mm* 60mm*

* No additional cradles are required for use with this positioner

Applications of RF Coils

SurfaceDual-Tuned

VolumePhased Array

Millipede

Oncology ✓ ✓ ✓

Spectroscopy ✓ ✓ ✓

Neurology (Brain and Spinal)

✓ ✓ ✓ ✓

Cardiac Scanning ✓ ✓

Micro-Imaging ✓ ✓

Whole Body Anatomic Scanning

✓ ✓

VisualSonics

Vevo2100 System& Vevo LAZR Image

System

SI healthcareJangwoo Cho

v1.0

<High frequency Preclinical Ultrasound System & Photoacoustic Image

System>

Functional/Physiological

Anatomical

Contrast

Image-Guided

Techniques

One Platform, Multiple Applications

High frequency Preclinical Ultrasound System

Superior resolution and image

uniformity throughout the Entire

field of view

30 micron resolution

Wide field of view

Medical grade monitor provides

higher resolution,

non-glare view from any angle

Photoacoustic Imaging Platform

Technical Specifications:

• Real-time, in vivo imaging of deep tissue

(up to 1 cm)

• Integrated, 20Hz tunable laser

(680-970 nm)

• Resolution down to 45 µm

• Imaging through endogenous

hemoglobin signal

• High optical contrast co-registered with

high-resolution imaging

Imaging station

Mouse & Rat Table

Temperature controlled platform maintaining

optimal physiological conditions for small animals

Integrated & displayed physiological monitoring –

ECG, heart rate, core temperature,

respiration

Transducer mounting system – for precision,

accuracy and hands – free scanning

3D positioning system

Accessory

Anesthesia System for Animal

Image Acquisition

Vevo MicroScan

MS700 : 70 MHz MicroScan transducer

- Broadband Frequency: 30 MHz - 70 MHz

- Applications: mouse embryology, epidermal imaging, superficial

tissue, subcutaneous tumors (< 9 mm), mouse vascular, ophthalmology

- Max Frame Rate: 476 fps

- Image Width: 9.7 mm, Image Depth: 12 mm

- Image Axial Resolution: 30 �m

Vevo LAZR

LZ250 : 13-24 MHz Integrated Fiberoptic Transducer

- Integrated fiberoptics for pulsed laser light delivery

- 256 sensitive piezoelectric elements for acoustic detection

- Broadband Frequency: 13 MHz - 24 MHz

- Image Width: 23 mm, Image Depth: 30 mm

- Image Axial Resolution: 75 �m

LZ550 : 32-55 MHz Integrated Fiberoptic Transducer

- Integrated fiberoptics for pulsed laser light delivery

- 256 sensitive piezoelectric elements for acoustic detection

- Broadband Frequency: 32 MHz - 55 MHz

- Image Width: 14.1 mm, Image Depth: 15 mm

- Image Axial Resolution: 40 �m

• LV Function

• Cardiac Output

• FractionalShortening

• VTI Interval

• Valvular Stenosis

• Vital Signs/BP integration

• RespiratoryGating/ECG

• Embryon lethal viability

• Great Vessels image and Dopplermeasurement

• Monitor Surgical sutures/hypertension

• Plaqueprogression

• Renal insuffiency

• Targetedcontrast to VCAM, P-Selectin

• Tumor size volumes

• Quantify relative vascular flow

• Monitor tumor metastasis

• TargetedMolecularImaging

• VEGFR2

• 3-D Modeling

• Injections

• Track Pharmaco-dynamic effects of novel compounds in real time

• Organ System Imaging

• Organ Blood flow at 30 microns

• Evaluate Path physiologiceffects of novel compounds

• Cardiac Function

• Renal Function

• Inflammation with molecular targets

• Injections and biopsy

• Tissue stiffness with DRF

• MonitorInflammation with molecular targets

• Injections

• Image joints

• QuantifyExpression with targetedcontrast from biotinilatedligand of choice

Ultrasound

Cardiovascular Oncology Metabolic Toxicology Immunology

Bridging Multiple Therapeutic Areas

Embryo through to adulthood

AdultEmbryo

Phenotypic Screening from Embryonic Stage to Adulthood

Video Loops

Overview of Research Areas

• Cardiovascular Research• Cancer Research• Abdominal & Reproductive Imaging• Developmental Biology• Photoacoustic Imaging

Cardiovascular Research

Parasternal Long Axis

Video Loop

Parasternal Short Axis

Video Loops

Carotid Artery

Video Loops

10 w ApoE/LDLr DKO mouse CCA

80 w ApoE/LDLr DKO mouse CCA

1 mm

40 w ApoE/LDLr DKO mouse CCA

Image sequence courtesy of Gan et al, University of Gothenburg, Sweden

Plaque Build-up in Carotid Arteries in Adult Mouse

Cancer Research

• Screen for pre-palpable tumors• Quantification in 2D and 3D• Quantification in vascularity using

� Color Doppler� Power Doppler� Contrast Agents (MicroMarker)

• Quantification of Biomarkers (VEGFR2, Integrins…)

Cancer ImagingOrthotopic Tumor – B-Mode Imaging

*Images and histology courtesy of Dr. Marris & Nick Rhodin, Children's Hospital of Philadelphia

Pre-PalpableTumor (60 mm3)

Earliest PalpableTumor (180 mm3)

Cancer ImagingSubcutaneous Tumor – 2D B-Mode Imaging

Cancer ImagingSubcutaneous Tumor – 3D B-Mode Imaging

Volume = 115.95mm2

Video Loop

Vevo MicroMarker Contrast Agents

Exclusive Partnership:Bracco Group

Joint development and manufacturing of MicroMarker™ kits

Video Loop

Abdominal & Reproductive Imaging

• Midline Abdominal Landmarks • Adrenal Glands• Kidney• Pancreas• Ovary

Midline Abdominal Landmarks

Video Loop

Adrenal Gland

Video Loops

Mouse Left Kidney

Video Loop

Developmental Biology

1 mmAmnioticCavity

CoelomicCavity

EctoplacentalCavity

Atlas of Mouse Development, MH KaufmanFoster FS et al., Ultrasound in Med and Biol, 28:1165-1172, 2002

Embryonic Mouse Development at E7.5

Sagittal Profile at E12

Video Loop

Color Doppler in Placenta at E12

Video Loop

Injection into brain at E12

Video Loop

Profile at E17

Video Loop

Color Doppler demonstrating blood flow through the umbilical cord

Video Loop

Color Doppler – Umbilical Cord

Photoacoustic Imaging

Pulsed Laser Illumination

OpticalAbsorption

Heating of Light Absorbing Volume

ThermalExpansion

Pressure Waves

AcousticDetection

Optical Path Sound Path

Impactful images with the sensitivity of optical imaging and the resolution of

ultrasound

Novel features of Vevo LAZR system

• Inherent co-registration of photoacoustic and anatomical images� Simultaneous registration of photoacoustic image on 2D and 3D planes� Real-time acquisitions for true in vivo monitoring

What is this signal?

Where is this signal?

Needle SkinTumorNeedle SkinTumor

Delivery of nanoparticlesinto tumor

Microvascular Imaging –Mouse skull, superficial cortex

Inherently co-registered high-resolution anatomical image and high-sensitivity photoacoustic signal acquired transcranially in vivo from the mouse superficial cortex (skull and skin intact, hair removed)

Sensitive photoacoustic signal acquired transcranially in vivo from the mouse superficial cortex (skull and skin intact, hair removed). Projection of 3D data, C-Scan shown.

Hindlimb ischemia

3D images of mouse hindlimb during

ischemia Unilateral hindimb ischemia: model of peripheral arterial disease (PAD)-low mortality rate-ease of access to femoral artery

3080 yonge street suite 6100box 66 toronto canada M4N 3N1

US

100 park avenue, suite 1600new york, NY 10017

Europe

Science Park 4061098 SM AMSTERDAMThe netherlands

www.visualsonics.com

+1 416 484-5000

TOLL FREE >

1.866.416.4636 (north america)

+ 800 0751.2020 (europe)

Advancing preclinical research

CardiovascularCancerDevelopmental BiologyDiabetesNeurobiologyReproductive BiologyRegenerative MedicineOphthalmologyMolecular ImagingOrthopedicGene Delivery

Thank You

VISUAL SONICS Korea: SI Healthcare – Mr. Jangwoo Cho

jangwoo.cho@sihealthcare.co.kr

seihee.hwang@sihealthcare.co.kr

실험동물용 고해상도 디지털 엑스선 CT 영상 솔루션

NanoFocusRay Co., LTD.

June 2011

NFR Polaris-G90

전임상소동물용 in-vivo마이크로 CT

SPECIFICATIONS

X-ray sourceSealed type microfocus tube20 ~ 90kvP, 180uA, Max. 8Watts

X-ray detectorFlat Panel Detector 145x115 mm, 74.8 um Pixel size

Scanning time < 15sec for 360views

Magnification 1.4x ~ 10x, variable zooming mechanism

FOV Transaxial ~100mm, length ~200mm120mm large bore size

Rotation method 360°Continuous rotation using by Slip-Ring

Resolution < 13um voxel size

ReconstructionVolumetric Cone-beam Reconstruction (FDK)In-line / Off line mode

Radiation safety< 1uSv/h at any point on the instrument surface

Size / Weight 1250(W)x1450(D)x1600(H) mm, 350kg

Precision Gantry Rotation SystemHigh speed cone-beam reconstruction

X-ray 튜브Micro-focus X-ray tube

X-ray 검출기Flat Panel Sensor

샘플 스캔 메카니즘

Gantry rotation

배율가변 메카니즘

Slip-ring technology

호흡마취 시스템

호흡 및 심장 게이팅 시스템 (Optional)

제어시스템

System control and image acquisition

영상재구성 모듈

Reconstruction, 3-D viewer and image analysis

CabinetRadiation shield cabinet (1uSv/h)

NFR Polaris-G90 장비 구성

샘플 스캔 메커니즘

Variable zooming mechanism 적용

가변 배율 : 10x ~ 1.4x

FOV : transaxial 100mm ~ 14mm

직경 10mm 이내의 적출 시료

~ 직경 100mm 크기의 소동물까지 촬영 가능함.

Field of views (FOV) : Full body scan

Up to length 200mm

배율에 따른 FOV와 Voxel size ( 1 scan 수행 시)

배율FOV (mm)

[transaxial x length]Voxel Size (um)

[X x Y x Z]

1.4x 100.2 x 82.1 101.4x80.1

2.0x 70.1 x 57.4 71.0x56.1

4.1x 34.2 x 28.0 34.6x27.4

10.0x 14.0 x 11.5 14.2 x 11.2

Physiological Monitoring & Gating

Small animal gas anesthesia systemQuickly anesthesia for rats and mice use with isofluraneEase of anesthetic control

Gas anesthesia Mask ; keep an inactive of sleeping stateVisual monitoring using by CCD camera with IR LEDs

Allows the operator to see a real-time image of the animal during scanningThe gating software analyzes the 30frames/sec video stream and provide a respiratory gating signal for synchronized scanning.Method of generating respiration gating signals for x-ray micro computed tomography scanner (Korean patent application 10-2010-0004635)

Carbon WireElectrode

CCD camera

Video motiondetection

Respiratory & Cardiac Gating

NFR Polaris-G90 제품 구성

제품 구성품

X-ray Micro-CT

소동물용 호흡마취 마스크 1set

소동물용 베드 1set

소동물용 마취 시스템 / 예비마취챔버 1set

호흡 게이팅 시스템 1set (Optional)

사용자 매뉴얼(CD 포함)

설치 사양

정격전압 - AC 220V/60Hz 10A, 접지, 별도의 단자함 설치

외형치수 - 1300(W)x1420(D)x1700(H)mm

제품무게 - 350kg

Kwon-Ha Yoon, et al., “Articular Cartilage Imaging by the Use of Phase-contrast Tomography in a Collagen-Induced Arthritis Mouse Model”, Academic Radiology (2010)

Kwak Han Bok et al., “Inhibition of osteoclast differentiation and bone resorption by Rotenone, through down-regulation of RANKL-induced c-Fos and NFATc1 expression”, Bone (2009).

Jaemin Oh et al., “Risedronate Directly Inhibits Osteoclast Differentiation and Inflammatory Bone Loss”, Biol. Pharm. Bull. (2009).

Kang Y, Choi M, Lee J, Koh GY Kwon K, et al., “Quantitative Analysis of Peripheral Tissue Perfusion Using Spatiotemporal Molecular Dynamics”, PloS ONE (2009).

Hye Won Kim, Kwon-Ha Yoon, et al., “Micro-CT Imaging with a Hepatocyte-selective Contrast Agent for Detecting Liver Metastasis in Living Mice”, Academic Radiology (2008).

Chul Ho Jang, et al., “Mastoid obliteration using a hyaluronic acid gel to deliver a mesenchymal stem cells-loaded demineralized bone matrix: An experimental study”, International Journal of Pediatric Otorhinolaryngology (2008).

Quan-Yu Cai, Kwon-Ha Yoon, et al., “Colloidal Gold Nanoparticles as a Blood-Pool Contrast Agent for X-ray Computed Tomography in Mice”, Investigative Radiology (2007)

Lucia Martiniova, Karel Pacak, et al., “In vivo micro-CT imaging of liver lesions in small animal models” Methods (2009)

NFR micro-CT를 이용한 연구

주 요 고 객

설치예정

Image Gallery

32

Dental Application

Spine Image

Spine Bone fusion in Rat

35

Anatomical Record,2002

Mouse Inner Ear by micro CT

BA-1 A-2

3D Volume Rendering & CPR

A. Volume of left atrium (LA), B. Volume of left ventricle (LV), C. Volume of right atrium (RA), D. Volume of right ventricle (RV)

A B C D

LA LV RA RV

Vol.(cm3) 0.747 0.966 0.675 0.763

3D Volume Measurement of each cardiac chamber

BA

Plaque Analysis

Aortic Aneurysm Model of Mice

주식회사나노포커스레이

전북전주시덕진구팔복동 2가전북테크노파크테크노빌 B-114Tel. (063) 262-6795, Fax. (063) 262-6796

김경우(010-7504-8811, kwkim@nanofocusray.com )www.nanofocusray.com

Thank You!

philips

BV-Pulsera

I. System overview

philips

BV-Pulsera

1. Battery management

: 만약 energy level 이 특정한 %아래로 떨어지면, C-arm stand 에

경고표시가 나타난다. . C-arm stand 와 mobile view station 둘 다

연결되어 있어야만 하며 mobile view station 은 main power outlet

socket 에 연결되어 있어야만 한다.

이것은 재충전을 하기 위해서이며 C-arm stand 의 왼쪽 핸들 쪽에

connector panel 에서 오렌지색 불빛이 나타난다..

2. System ON/OFF

1) Switching the system on

C-arm stand 이나 mobile view station 위의

system on key 를 누른다.

2) Switching the system off

mobile view station 위의 system off 를 누른다.

philips

BV-Pulsera

단, c-arm 의의 c-arm stand off 만 누르면 오직 c-arm

stand 만 꺼진다.

3) Emergency Power Off

: 응급상황이 발생하였을 경우, 모든 작동을 멈추게 한다.

하지만, mobile view stand 의 main power plug

를 제거 해 주어야 한다.

3. Managing patients and examination

: 환자와 검사는 ‘Administration’ screen 에서 조작 할 수 있다.

philips

BV-Pulsera

Schedule – 검사를 위해 예약된 환자 확인

Review – 시스템에 저장되어 있는 획득된 검사 확인

4. Adding a new examination

1) ‘Administration’ screen 에서 ‘schedule’버튼을 누른다.

2) ‘Add’ 버튼을 누른다.

3) keyboard 를 사용하여 ‘Patient name’영역에서 환자의 이름을

넣고 tab key 를 눌러서 다음 영역으로 이동한다.

4) ‘Patient ID’에 환자 ID 를 넣고 tab 을 눌러서 다음으로 이동한다.

5) ‘Date of Birth’에 환자의 생년월일을 넣는다.

6) ‘Sex’에 성별을 입력한다.

7) ‘Type’에서 메뉴를 내려서 검사 type 을 선택한다.

8) ‘Physician’에서 메뉴를 내려서 의사 이름을 선택한다.

9) 새로운 검사를 확인하기 위해 OK 버튼을 누른다.

5. modifying and Deleting an examination

: schedule list 와 review list 에서 검사는 수정할 수 있다.

list 에서 획득한 검사항목을 선택한 후 administration

screen 에서 modify 또는 delete 할 수 있다.

6. Selecting a patient for acquisition

philips

BV-Pulsera

1) schedule list 로부터 환자를 선택한다.

2)start examination 버튼을 누른다.

3)선택된 검사는 acquisition 검사가 된다.

4) Administration screen 는 검사 모니터 위에 black screen 에 의해

재배치 된다. 환자의 이름은 스크린의 가운데에 나타나고

acquisition 이 준비된다.

7. Standard DICOM export tasks

1) ‘Administration’스크린에서 ‘Export’를 클릭한다.

2) 원한다면 ‘Accession Number’ Field 에서 환자를 선택하기 위해

Accession number 를 넣을 수 있다.

3) ‘Target’ list 로부터 필요한 network 장치를 선택한다.

4) ‘Image Selection’ option 에서 Image 를 선택한다.

5) ‘Image format’ option 에서 필요한 format 을 선택한다.

6) 모든 setting 이 올바를 때 ‘ok’버튼을 클릭한다.

II. Image Essentials

philips

BV-Pulsera

1) Image Intensifier size 변경

또는 remote control 에서 변경한다.

2) Contrast and Brightness 조정

감소 증가

되돌리기

philips

BV-Pulsera

- contrast 와 brightness 기능을 자동으로 조절하기 위해

“Auto” 키를 누른다.

3) Rotate Image

얻고자 하는 곳까지 이미지를 회전하기 위해서 key 를 계속 누르고,

원래의 위치로 이미지를 복구 시키기 위해서 “0”점을 누른다.

4) Mirror Image

Image 를 좌우 또는 위 아래로 돌린다.

5) Iris and Shutter Adjustments

원하는 shutter 를 선택하여 누른다.

두 가지 shutter 모두를 조정하려면 두

개의 key 를 동시에 누른다.

philips

BV-Pulsera

선택된 shutter 회전, reset 은 “0”점을

누른다.

iris 를 안쪽 혹은 바깥쪽으로 이동,

reset 은 “0”점을 누른다.

III. X-ray control

philips

BV-Pulsera

1) Automatic kV/mA control

: 초기상태에 의해 이미지 밝기는 kV 와 mA 값을 바꿈으로써

자동으로 조절할 수 있다.

2) Manual kV/mA control

: 자동제어를 수동으로 바꿀 수 있다.

(kV 버튼을 누른다.)

3) Automatic mA increase in LDF mode

: 이것 또한 High penetration mode (HIP)라 부른다.

시스템이 가장 높은 kV 값에서 좋은 이미지를 만들어 낼 수 없을

때, 더 높은 mA 값에 의해 자동 조절 될 것이다..

(한계 시간은 30 초이다. 이 시간 후에 시스템은 normal mA 로

스위치가 돌로 돌려진다.)

IV. Making Image

philips

BV-Pulsera

1. Making a Low Dose Fluoroscopy (LDF) image

: LDF imaging 은 외과 수술 진행 동안 안내하고 C-arm 의 위치를

조정하는 목적을 위해서 추천한다.

1/4dose 와 1/2 dose LDF

1/4dose 또는 1/2 dose LDF 는 C-arm stand console 에 'X mode'의

soft key 를 사용해서 선택될 수 있다. 이러한 기술은 더 적은 이미지를

획득함으로써 dose 를 saving 하는 기술이다.

• 1/2 dose - 12.5 fr/s

• 1/4 dose - 6 fr/s

: LDF 이미지를 만들기 위해서, 왼쪽 hand switch key

또는 foot switch 의 왼쪽 pedal 을 눌러라.

(Storage speed 가 0 일 때, LIH 는 다음 LIH 에 의해 겹쳐질 것이고

disk 에 저장되지 않는다.)

image 는 Live monitor 에 나타난다. 다음 run 을 시작하면

image 는 다시 LIH 될 것 이다.

LDF 동안 다음 이미지가 보여지고 저장하기 위해서

remote control 또는 mobile view station 에서 Grab key 를 눌러라.

그럼 새로운 run 이 발생하여도 저장 되어 진다.

2. Making high quality images

philips

BV-Pulsera

: Hand 스위치의 오른쪽 key 또는 foot 스위치의 기능은 선택된

examination type 과 technique 에 의해 자동으로 결정된다.

*exposure technique

- digital exposure

- pulsed exposure

- radiography

*fluoroscopy technique

- high definition fluoroscopy

: Switching X-ray mode - X-ray mode 는 항상 바꾸는 것이 가능하다.

'X-mode' soft key 를 눌러서 원하는 mode 를 선택해라.

: Switching techniques - Fluoroscopy 와 Exposure technique 은 항상

바꾸는 것이 가능하다. 'Technique' soft-key 를 눌러서 바꾸어라.

: Storage - LIH 의 storage speed setting 에 관계없이 항상 disk 에

저장할 수 있다.

(1) Digital Exposure (SharpShot)

: Digital exposure (또한 SharpShot 이라고도 한다) 는

고화질로 저장하는데 사용된다.

1) 'X mode' key 를 눌러서 Digital exposure 를 선택해라.

2) single exposure 가 나오기 위해서 hand 스위치의 오른쪽 key

또는 foot 스위치의 오른쪽 pedal 을 눌러라.

philips

BV-Pulsera

kV control: digital exposure 동안 kV 와 mA 값은 고정되어 있다.

최적의 이미지 quality 를 확인하기 위해서 LDF 를 사용하여 scout

이미지를 만들고 적당한 kV 값을 정해라.

(2) Pulsed exposure

: Pulsed exposure 는 dynamic 이미지에 사용된다.

1) 'X-mode' soft key 를 누르고 'pulsed'를 선택해라.

2) 미리 조정된 pulse frequency 와 함께 pulsed exposure

sequence 를 시작하기 위해서 핸드스위치의 오른쪽 key 를

누르거나 foot 스위치의 오른쪽 pedal 을 눌러라.

pulse frequency : pulse frequency 를 바꾸기 위해서, 'Speed' key 를

눌러서 원하는 frequency 를 선택해라.

fr/s Max run time Max images in one

run

30 15s 450

15 30s 450

philips

BV-Pulsera

8 30s 240

5 30s 150

3 30s 90

(3) Pulsed fluoroscopy

: Pulsed fluoroscopy 는 dose saving mode 이거나 positioning

catheters 그리고 해부학상의 지역에서 빠르게 움직이는 장치를 위해서

움직임을 멈춰주는 mode 이다.

hand 스위치의 왼쪽 key 이나 foot 스위치의 왼쪽 pedal 을 눌러서

작동 되어진다.

(4) High Definition Fluoroscopy (X-ray mode continuous)

: Definition Fluoroscopy (HDF) 이미지는 저장하거나 고화질의 이미지를

만들 수 있다.

1/4dose and 1/2 dose HDF

1/4 dose 또는 1/2 dose HDF 는 C-arm stand console 에 'X mode'

soft key 를 사용하여 선택할 수 있다. 이 기술은 더 적은 이미지를

얻음으로써 dose 를 saving 하는 기술이다.

• 1/2 dose - 12.5 fr/s

• 1/4 dose - 6 fr/s

HDF 이미지를 만들기 위해서, 핸드스위치의 오른쪽 키 또는

foot switch 의 오른쪽 pedal 을 눌러라.

philips

BV-Pulsera

note: HDF acquisition run 은 30 초 후에 자동으로 끝난다.

3. Making vascular images

: Vascular image 를 얻기 위한 Examination type 을 선택한다.

그러면 'Mode' soft-key 는 사용 가능해지고 subtraction, trace,

roadmap, normal fluoroscopy 사이에서 선택이 가능해진다.

note

: vascular 이미지는 일반적인 fluoroscopy 가 선택된 이외의 mode 와

vascular 검사 type 일 때만 오직 가능하다.

(1) Performing subtraction

① 'Mode' soft key 를 누르고 나서 'Subtr'을 선택해라.

or 'Subtr' mode 를 고정시키기 위해서 foot 스위치의 중간 pedal

을 눌러라.

or 'Subtr' mode 를 고정시키기 위해서 remote control 에 |Mode|

key 을 눌러라.

mode 는 모니터의 오른쪽 아래 코너에 나타난다.

· storage frequency 를 바꾸기 위해서 'Speed'의 soft key 를 누르고

원하는 frame 수를 선택해라.

philips

BV-Pulsera

· HDF 는 subtraction 을 위해 더 좋은 이미지 quality 를 공급하기

때문에 LDF 보다 더 선호된다.

·subtraction 과정 동안 환자나 시스템을 움직이지 마라.

blurred 이미지가 발생할 수 있다.

② hand switch 오른쪽이나 foot switch 의 오른쪽 pedal 을

누른다.

③ mask 의 완료 후에 이미지는 회색으로 바뀌고 모니터에 'Inject'

메시지가 나타난다.

note

: subtraction 이 완전히 끝나기 전까지 hand/foot 스위치를 떼지 마라.

hand/foot 스위치가 해제될 때, 새로운 mask 가 fluoroscopy 가 다시

실행된 후에 즉시 만들어진다.

④ 조영제를 써서 injecting 을 시작해라. contrast bolus 의 이미지가

모니터에 나타날 것이다.

⑤ contrast bolus 가 사라지자 마자 Hand 또는 Foot 스위치를

해제해라

note

: 실행되는 각각의 이미지를 보기 위해서 mobile view station 에서

|Previous| 그리고 |Next| key 를 사용하거나 remote control 에서

아래의 버튼을 사용해라.

얻어진 이미지들을 확인하기 위해서 mobile view station 에서

|Run cycle| key 또는 remote control 에서 아래 버튼을 눌러라.

philips

BV-Pulsera

(2) Performing roadmap after subtraction

① 이전에 얻은 subtraction run 으로부터 roadmap 으로 사용하기 위한

이미지를 선택해라.

Or 'Mode' key 를 누르고 'Roadmap'을 선택해라.

Or 'Roadmap' mode 를 고정하기 위해서 foot 스위치의 중간 pedal 를

눌러라.

② 'Roadmap' mode 를 고정하기 위해서 remote control 에 |Mode| key

를 눌러라.

mode 는 모니터의 오른쪽 아래 코너 쪽에 나타난다.

③ 핸드스위치의 왼쪽 key 나 foot 스위치의 왼쪽 pedal 을 눌러라.

: 시스템은 roadmap 으로 스위치가 돌려진다. mask 이미지는 뒤집힐

것이고 혈관은 흰색으로 보여진다. 'Catheter' 메시지가 모니터에

나타난다. 이미지는 catheter 유도용으로 사용될 수 있다.

note

: 이전에 실행한 이미지를 사용할 때 BV Pulsera/patient 는

그사이에 움직여서는 안 된다.

'Roadmap' mode 에서 radiation 은 멈출 수 있고 mode 에서

나가지 않고 시작할 수 있다.

philips

BV-Pulsera

(3) Remasking

: 초기값에 의해 첫 번째 이미지 실행은 mask 로 사용된다. 그러나

mask 로써 같은 실행으로부터 다른 이미지를 사용하는 것이 가능하다.

① mobile view station 에서 |remask| key 를 눌러서

subtraction 을 꺼라.

② 새로운 mask 를 하기 위해 이미지를 선택해라.

③ subtraction 을 켜라.

: remote control 을 사용해서 remask 없이 subtraction 을 끄고 켤 수

있다. (새로운 mask 가 선택되어지지 않을 것이다.)

* SmartMask

: reference 모니터에서 이미지는 remote control 에 |SmartMask| key 를

눌러서 roadmap 으로서 사용될 수 있다.

Fluoro image 가 아닌 exposure image 를 roadmap image 로 사용할

수 있다.

(4) Bolus chase

① 'Technique' soft-key 를 눌러서 'Exposure'를 선택해라.

② 'X-mode' soft-key 를 눌러서 'Pulsed'를 선택해라.

③ bolus chase 를 위해서 초기 setting 값은 필요조건에 따라야 한다.

만약 아니라면, 'Speed' soft-key 를 누르고 올바른 pulse

frequency 를 선택해라.

philips

BV-Pulsera

④ 핸드스위치의 왼쪽 key 또는 foot 스위치의 왼쪽 pedal 을 누르고

bolus chase route 의 covering 을 확인하기 위해서 fluoroscopy 동안

지속적으로 C-arm 이나 tabletop 을 움직여라.

⑤ Hand or Foot 스위치를 떼고 bolus chase 의 시작위치로 C-arm 또는

tabletop 위치를 놓는다.

⑥ 핸드스위치 오른쪽 key 또는 foot 스위치 오른쪽 pedal 을 누르고 C-

arm 과 tabletop 을 지속적으로 움직이면서 inject 된 조영제를

따라간다.

⑦ bolus chase route 의 끝에서 exposure run 을 멈추기 위해 Hand or

Foot 스위치를 해제 한다.

Tip

- Leg coverage

: 12" II 는 같은 시간에 2 개의 leg 을 cover 하는 것이 가능하다. 9" II 는

오직 하나의 leg 만 cover 하는 것이 가능하다.

philips

BV-Pulsera

4. Standard DIOCM package

:examination image 를 저장하기 위해서 병원이나 부서의 network 에 보내게

해줍니다. 이 option 을 사용하기 위해서는 기본적으로 병원이나 부서의

network 에 반드시 연결되어 있어야만 합니다.

1. ‘Administraion’ 스크린에서 ‘Export’를 클릭합니다.

2. ‘Export’ panel 이 나타납니다.

만약, 필요하다면 Auto matching 을 위해 ‘Accession Number’ field 에

Accession number 를 넣을 수 있습니다. 단, Name 과 Patient ID 는

조정할 수 없습니다.

3. ‘Target’ list 로부터 필요한 network 장치를 선택합니다.

4. ‘Image Selection’ 에서 원하는 Image 를 선택합니다.

(각각에서 image 수가 쓰여져 있습니다.)

5. ‘Image format’ 에서 필요한 format 을 선택합니다.

(기본적으로 XA 로 넘기시면 됩니다.)

6. 모든 셋팅이 올바르면 ‘OK’버튼을 누르시면 됩니다.