MAGNETIC AND NON-MAGNETIC PARTICLESRAW MATERIALS FOR IVD ASSAY DEVELOPMENT

Quality, Performance, Service

Agilent LodeStars

• Controlled surface functionality - highly selective ligand capture

• Low backgrounds - excellent performance

• Rapid magnetic response• Uniform, monodispersed - batch-

to-batch reproducibility

Learn more:www.agilent.com/chem/beads

The selection of a reliable supplier, enabling you to have the utmost confidence in every detail of your products, is essential. Partnering with Agilent gives you:

Supply management

Scheduled deliveries and worldwide logistics minimize expense and risk, ensuring your beads are in the right place, at the right time. Inventory management provides batch and shelf life control.

Flexibility Custom quality and supply agreements. Small to multikilogram batch sizes.

Quality Beads with demonstrated performance, manufactured reproducibly, to a robust process in an ISO 9001 facility.

Expertise Access to 40 years' experience in the manufacturing and development of innovative, high-technology products.

Agilent engaged a contract research organization to conduct a non-clinical performance study of particle performance using a commercially available i-PTH assay kit.

The kit is designed to measure the amount of i-PTH present in a sample; reference i-PTH was used in this study.

The study measured the fluorescence output of acridinium ester in a direct sandwich chemiluminescent assay, using an automated immunoanalyzer platform. The assay (performed in cuvette) comprised a biotinylated antihuman PTH antibody prebound to streptavidin coated beads. These were mixed with reference i-PTH sample in the presence of antihuman PTH antibody labeled with acridinium ester.

The assay used a constant amount of the antihuman PTH antibody labeled with acridinium ester and then varied different parameters as follows: - amount of magnetic beads (25 - 100 µg beads/cuvette)- amount of reference i-PTH (dose of 0-2400 pg/mL)- magnetic beads: LodeStar Streptavidin or Alternative 2.7 µm bead

Research study demonstrating the performance of Agilent LodeStars against alternative magnetic particles

For Research Use Only. Not for use in diagnostic procedures.Information, descriptions and specifications in this

publication are subject to change without notice.

© Agilent Technologies, Inc. 2016Published in USA, August 23, 2016

5991-7189EN

To find out more, visit: www.agilent.com/chem/beads

Description Volume Part No.

LodeStars Streptavidin

2 mL PL6727-1001

10 mL PL6727-1003

100 mL PL6727-1005

800 mL PL6727-1007

LodeStars Carboxyl

2 mL PL6727-0001

10 mL PL6727-0003

100 mL PL6727-0005

400 mL PL6727-0006

800 mL PL6727-0007

Quality, Support, and Global Logistics

Agilent’s ISO 9001 certified, state-of-the-art, manufacturing facility in Church Stretton, Shropshire, UK manufactures magnetic and non-magnetic particles from small to multikilogram batch sizes. Agilent provides the support and documentation required for the raw materials used by you in the development and commercialization of immunodiagnostic assays.

PL-Latex

Polystyrene-based uniform latex particles designed for research and for diagnostic tests. A range of particle sizes (50–1000 nm), colors, carboxyl variants and customization is available.

For more information, visit: www.agilent.com/chem/beads

LodeStars

LodeStars are Agilent’s proprietary high performance superparamagnetic particles designed for biomagnetic separations. They are available in two standard formats (Streptavidin and Carboxyl) and custom variations meaning you can easily optimize your assays.

Specifications

Diameter 2.7 µm

Iron content 20%

Magnetic mass susceptibility > 60 m3/Kg

Settling rate (under gravity)* > 1.5 cm

Magnetic response > 20 s

Streptavidin binding capacitiesBiotin > 1,200 pmoles/mg bead

Biotinylated IgG > 5.0 µg/mg bead

Carboxyl binding capacitiesImmno-capture of mouse IgG-OG** > 4.0 µg/mg bead

* Technique: Visual inspection of clear supernatant height after settling under gravitational forces for a concentration of 10 mg/mL magnetic beads and 4 mL volume in: 0.1% Tween 20, 0.1% BSA, PBS pH 7.4, and 0.1% sodium azide.

** Assay: Goat anti-mouse lgG bound to Oregon Green-labeled mouse lgG at saturation level (40 µg each/mg bead). Experiment details available on request.

LodeStars are monodispersed and have good size distribution.

Agilent has a wealth of expertise in particles and chemistry modifications, backed up with a proven track record in the manufacturing and development of innovative and high technology polymer products for 40 years.

By partnering with Agilent, you benefit from security of supply, global logistical capabilities, certified quality standards, and industry experience.

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