M-Phase• Prophase – Metaphase – Anaphase – Telophase

• Prophase – chromatin begins to tightly coil (condenses)– nuclear envelope begins to disappear – nucleoli disappear – each chromosome is now a complex of sister chromatids – mitotic spindle begins to form – centrosomes move away fro each other

• Prometaphase – nuclear envelope fragments – microtubules move through the nuclear area through

the center of the cell – each sister chromatid complex is now connected to a

microtubules by the kinetochore region of the centromere

M-Phase• Metaphase

– longest phase of Mitosis– centrosomes are now at opposite

sides of the cell – chromosomes are located at the

metaphase plate (center) – kinetochores of the sister

chromatids are now attached to tubules of opposite centrosomes

– microtubule complex is now called the spindle

M-Phase• Anaphase

– shortest phase of Mitosis– sister chromatids are pulled apart

as the spindle fibers shorten – the separated chromosomes

migrate toward the opposite poles

– at the end of anaphase the polar regions of the cell have equivalent amounts and types of chromosomes

M-Phase• Telophase

– the 2 daughter cell nuclei begin to form

– nuclear envelopes begin to form from the parent fragments

– chromosomes become less condensed

– mitosis is complete with the completion of the 2 nuclei regions

M-Phase

• Cytokinesis – the cell divides at the cleavage

furrow shortly after Mitosis • Pinching in of the plasma

membrane

– Cell plate forms in plants• Becomes the cell wall