M I C R O B I O L O G Y...
Transcript of M I C R O B I O L O G Y...
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M I C R O B I O L O G YWITH DISEASES BY BODY SYSTEM SECOND EDITIONMIKROBIOLOGI
YUSRON SUGIARTOLESSON 1
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NO MATERI
1 PENDAHULUAN
2 PROKARIOTIK
3 EUKARIOTIK
4 VIRUS
5 TEKNIK ISOLASI
6 PERHITUNGAN MIKROBA
7 IDENTIFIKASI MIKROBA
MATERI SEBELUM UTS
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REFERENCES
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Microorganisms were the first life on earthMicroorganisms created the biosphere that allowed multicellular organisms to evolveMulticellular organisms evolved from microorganisms>50% of the biomass on earth is comprised of microorganismsMicroorganisms will be on earth forever
WHY STUDY MICROBIOLOGY?
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•Our understanding of life has arisen largely from studies of microorganisms (biochemistry and genetics)•Studies of microorganisms continue to contribute to fundamental knowledge of life processes•We still know very little about the microorganisms that are present on Earth
WHY STUDY MICROBIOLOGY?
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WHY STUDY MICROBIOLOGY?
Health
Agriculture
Food
Environment
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•The science of microorganisms (very small, unicellular organisms)•The discipline is just over a century old•Has given rise to molecular biology and biotechnology
WHAT IS MICROBIOLOGY?
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THE MICROBIAL WORLD
•Microorganisms are everywhere: air, soil, water, plants, animals
Microbial life is diverseBacteria have existed for 3x109 yearsEvolved to flourish in the most inhospitable environments
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WHAT ARE MICROBES?
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EVOLUTIONARY TIME LINE
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BASIC STRUCTURE OF CELLS AND VIRUSES
(a) Comparison of a prokaryotic cell and a eukaryotic cell.
(b) Two examples of viruses.
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Carolus Linnaeus (Swedish) developed taxonomic system for naming plants and animals and grouping similar organisms together
Leeuwenhoek’s microorganisms grouped into six categories as follows:
FungiProtozoaAlgaeBacteriaArchaeaSmall Animal
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THE SIX BASIC TYPES OF MICROORGANISMS
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THE SIZES OF THE SMALLEST ORGANISMS AND OBJECTS
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• Eukaryotic (have membrane-bound nucleus)
• Obtain food from other organisms
• Possess cell walls• Composed of
• Molds – multicellular; have hyphae; reproduce by sexual and asexual spores
• Yeasts – unicellular; reproduce asexually by budding; some produce sexual spores
FUNGI
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• Single-celled eukaryotes• Similar to animals in nutrient needs
and cellular structure• Live freely in water; some live in
animal hosts• Asexual (most) and sexual
reproduction• Most are capable of locomotion by
• Pseudopodia – cell extensions that flow in direction of travel
• Cilia – numerous, short, hairlike protrusions that propel organisms through environment
• Flagella – extensions of a cell that are fewer, longer, and more whiplike than cilia
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• Unicellular or multicellular• Photosynthetic• Simple reproductive
structures• Categorized on the basis of
pigmentation, storage products, and composition of cell wall
ALGAE
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• Unicellular and lack nuclei• Much smaller than eukaryotes• Found everywhere there is
sufficient moisture; some found in extreme environments
• Reproduce asexually• Two kinds
• Bacteria – cell walls contain peptidoglycan; some lack cell walls; most do not cause disease and some are beneficial
• Archaea – cell walls composed of polymers other than peptidoglycan
BACTERIA AND ARCHAEA
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THE SCOPE OF MICROBIOLOGY
• Microbiology: The study of living things too small to be seen without magnification
• Microorganisms or microbes- these microscopic organisms
• Commonly called “germs, viruses, agents…” but not all cause disease and many more are useful or essential for human life
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THE GOLDEN AGE OF MICROBIOLOGY
Antoni van LeeuwenhoekInventor of the first microscope (1684)
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THE GOLDEN AGE OF MICROBIOLOGY
• Redi’s Experiments
• When decaying meat was kept isolated from flies, maggots never developed
• Meat exposed to flies was soon infested
• As a result, scientists began to doubt Aristotle’s theory
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The Golden Age of Microbiology
• Pasteur’s Experiments
• When the “swan-necked flasks” remained upright, no microbial growth appeared
• When the flask was tilted, dust from the bend in the neck seeped back into the flask and made the infusion cloudy with microbes within a day
THE GOLDEN AGE OF MICROBIOLOGY
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Germ Theory of Disease
•Proof that microorganisms caused disease•Robert Koch demonstrated that anthrax was caused by Bacillus anthracis• Blood from a diseased animal caused disease in a
healthy animal • Cultivated the disease causing agent outside the animal’s
body, then introduced the agent into a healthy animal which subsequently developed the disease
THE GOLDEN AGE OF MICROBIOLOGY
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The Golden Age of MicrobiologyTHE GOLDEN AGE OF MICROBIOLOGY
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The Golden Age of Microbiology
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The Golden Age of Microbiology
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The Golden Age of Microbiology
• How Can We Prevent Infection and Disease?• Semmelweis and
handwashing• Lister’s antiseptic technique• Nightingale and nursing• Snow – infection control and
epidemiology• Jenner’s vaccine – field of
immunology• Ehrlich’s “magic bullets” –
field of chemotherapy
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The Modern Age of Microbiology
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The Modern Age of Microbiology
• What Are the Basic Chemical Reactions of Life? • Biochemistry
• Began with Pasteur’s work on fermentation and Buchner’s discovery of enzymes in yeast extract
• Kluyver and van Niel –microbes used as model systems for biochemical reactions
• Practical applications• Design of herbicides
and pesticides• Diagnosis of illnesses
and monitoring of patients’ responses to treatment
• Treatment of metabolic diseases
• Drug design
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The Modern Age of Microbiology
•How Do Genes Work?• Microbial genetics• Molecular biology• Recombinant DNA
technology• Gene therapy
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• Recombinant DNA Technology
• Genes in microbes, plants, and animals manipulated for practical applications
• Production of human blood-clotting factor by E. coli to aid hemophiliacs
• Gene Therapy
• Inserting a missing gene or repairing a defective one in humans by inserting desired gene into host cells
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• What Role Do Microorganisms Play in the Environment?
• Bioremediation uses living bacteria, fungi, and algae to detoxify polluted environments
• Recycling of chemicals such as carbon, nitrogen, and sulfur
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• How Do We Defend Against Disease?• Serology
• The study of blood serum• Von Behring and Kitasato
– existence in the blood of chemicals and cells that fight infection
• Immunology• The study of the body’s
defense against specific pathogens
• Chemotherapy• Fleming discovered
penicillin• Domagk discovered sulfa
drugs
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What Will the Future Hold?
• Microbiology is built on asking and answering questions
• The more questions we answer, the more questions we have
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WORLDWIDE INFECTIOUS DISEASES AFFECTING HEALTH SCIENCES
• Increasing number of drug resistant strains including Nosocomial and Community Acquired microorganisms
• MRSA Methicillin Resistant Staphylococcus aureus
• VREVancomycin Resistant
Enterococcus• VRSA
Vancomycin Resistant Staphylococcus aureus
• MDR-TB Multidrug Resistant Tuberculosis
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Worldwide Infectious Diseases Affecting Health Sciences
• Increasing number of emerging diseases (SARS, AIDS, hepatitis C, viral encephalitis)
• Other diseases previously not linked to microorganisms now are (gastric ulcers, certain cancers, multiple sclerosis)
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M I C R O B I O L O G YWITH DISEASES BY BODY SYSTEM SECOND EDITIONTHANK YOU
YUSRON SUGIARTOPERTEMUAN 1