Lupin Seed as replacement for Fishmeal in African Catfish (Clarias gariepienus) Feed

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    Use of Lupin Seed as Fishmeal

    Replacement in African Catfish

    (Clarias gariepinus) Feed

    AISHA MUKHTAR AMMANI (SI1079)Faculty of Bio-resources & Food Industry

    Supervisor: Associate Prof. Dr. Connie Fay Komilus

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    a!le of ContentsIntroduction

    "ro!lem Statement

    #!$ecti%es

    Scope

    Literature Re%ie&

    'pothesisAims

    *ethodolo+

    ,antt Chart

    -.pected #utcomes

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    a source ofhigh-quality

    protein,essential

    amino acids,

    oil and othernutritivesubstances.

    Introduction

    Fishmeal

    is a highprotein

    feed

    ingredient

    u!in (L.angustifolius" is alegume,

    thatbelongs to

    peasfamily

    *oti%ation

    Search for

    local & cheap

    alternative

    sources of

    protein

    Cat#s$(Clarias

    gariepinus"the mostimportanttropicalcatsh

    species foraquaculture.

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    Introduction

    u!in See%s (L. angustifolius"

    Anatomy of L. angustifolius

    Image source: http://www.lupins.org

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    Problem Statement

    Fishmeals are highly sought

    after as the protein source of

    choice for many formulated

    aquaculture diets.

    High contents of Amino &

    Fatty acids

    Lo !arbohydrates Lo anti"nutritional factors

    Hoever# the production of fishmeal

    uses appro$imately %' of the total

    global fish catch()*.

    A!out / + of &et fish is reuired

    to produce 1 + of dr fishmeal2

    hence the need for alternati%es to

    fishmeal.

    ()* +acon# A.,.-.# Hasan# ./. and Subasinghe# /.0. 123345. 6se of fishery resources as feed inputs for aquaculture development7 trends and

    policy implications. FA8 Fisheries !ircular 9o. :::# /ome# FA8. )33pp

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    8b;ectives

    To nd areplacement forepensive animalprotein sources

    with good qualityand least costplant proteinsources forenhanced sh

    production.

    To determine theoptimum inclusionlevel of !upin

    "eed #eal $!"#%in replacingshmeal informulated dietsof Clariasgariepinus.

    To evaluate thequality ofprepared feeds onthe basis of

    growthperformance ofClarias gariepinusfed with varyinginclusion levels of

    !upin seed meal.

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    Scope

    Pro$imate analysis of each feed ingredients using the methods

    described by A8A! )

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    Literature /evie

    Amisah et al. 1233 groth trial to evaluate the

    effects of a plant protein blend 1PPB# comprised of soybean meal and

    heat gluten meal5 as fishmeal 1F5 substitute on groth# nitrogen 195

    and phosphorus 1P5 balance in Siberian sturgeon#Acipenser

    baeriiBrandt. /esult indicates that groth performance as not affectedby dietary protein sources and protein levels. Fish fed the lo"protein

    diets had higher FI than that of the high protein groups# hile fish fed

    the F"based diets had higher FI than that of fish fed the PPB"based

    diets. &misah, "., 'teng, #. &( 'fori, ). *. $+%. rowth erformance 'f The &frican 0atsh, 0larias gariepinus, 1ed2arying 3nclusion !evels 'f !eucaena leucocephala !eaf #eal )ournal of &pplied "ciences 4 5nvironmental#anagement, 2ol. 67, 8o. 6, #arch, +, pp. +6-+9

    un, ;.,

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    Literature /evie

    Hairui et al. 123)C5 conducted a )3"ee> feeding trial to evaluate the

    effects of replacing fish meal 1F5 ith meat and bone meal 1B5 on

    groth and body composition of ;uvenile snea>ehead Ophiocephalus

    argus. +he results indicated that B could replace 23' of F protein

    in diets of sna>ehead ithout negative effect on groth and feed

    utili?ation.

    -irsa et al. 123)C5 conducted an "ee> feeding trial to determine the

    effectiveness of replacing fish meal 1F5 ith blends of alternative

    proteins in diets for hite seabass 1DSB#Atractoscion nobilis5 at astarting eight of .4g. +he study shos that performance decreases as

    inclusion of the soy"based protein blend increases.

    >airu, ., Ein, ?., >ui, 0., Tong, T., >uang, ., !i, =. $+6F%. Geplacement of sh meal by meat and bone meal indiets for @uvenile snaHehead 'phiocephalus argus, 1isheries "cience, 2ol. C6, 3ssue D, ppB+7-B+

    I. )irsa6, 1.T. ;arrows+, G.=. >ardy7 and #. Irawbridge $+6D%. &lternative protein blends as a replacement for shmeal in diets for white seabass, &tractoscion nobilis, &quaculture 8utrition 3""8: 679F-+F

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    Literature /evie

    /oghayeh et al. 123)5 conducted a feeding trial to evaluate the

    potential of replacing fish meal by processed blood meal# in fingerling

    rainbo trout Oncorhynchus y!iss diets. +he results shoed that

    blood meal is not a suitable protein source as fish meal replacement for

    fingerlings rainbo trout.

    @uyu et al. 123)5 conducted an "ee> feeding trial to evaluate the

    effects of replacing fishmeal ith soybean meal 1SB5 on groth# feed

    utili?ation# and nitrogen 195 and phosphorus 1P5 e$cretion of

    ;uvenile "seudobagrus ussuriensis. +he results shoed that there as

    no significant difference in eight gain among fish fed S3# S)3# S23#S%3 and SC3 diets# hoever# a significant reduction in this variable

    occurred hen 3' and 43' of fish meal protein as replaced by

    SB. Goghayeh ;ahrevar, >amid 1aghani-!angroudi( $+6F%. 5Ject of sh meal replacement by blood meal in ngerlingrainbow trout $'ncorhynchus myHiss% on growth and body/llet quality traits. &quaculture, &quarium 0onservation4 !egislation, 3nternational )ournal of ;ioKu "ociety 2ol. C, 3ssue 6

    uyu =ang6, "hiliang u6, ueyang =ang+, )ianfang 0he6, !iping ?hao6,

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    HypothesisEAims

    This research aim is to investigate the eMcacy of shmealreplacement with lupin seed to formulate feed withoutdetrimental impacts on growth in Clarias gariepienus.

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    ethodology

    "hase I3

    Pro$imate Analysis of feed

    Ingredients

    ). oisture

    2. Ash%. !rude Protein

    C. !rude Lipid

    . !rude Fibre

    4. 9itrogen"free e$tracts

    1digestible carbohydrates5

    "hase II3Formulation and Preparation of

    Fish Pellets.

    "hase III3 Fish /earing and Feeding +rials Sample !ollection and Analysis

    Specific ,roth /ate 1S,/5 Feed 0fficiency 1F05 Survival /ate 1S/5

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    "hase I3 "ro.imate Analsis of Feed

    In+redients

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    Phase I

    "ro.imate Analsis of Feed In+redients

    Air =ry Sample

    oisture"Free Sample

    !rude Protein Fat"Free residue

    4$eldahl56 -ther e.traction58

    -ther e.tract

    r at 10:0C

    ;oil in acid

    ;oil in alali

    !rude Fiber Ash!rude Fiber

    Ash

    ;urn

    in

    Furnace

    (2* Association of 8fficial Analytical !hemists 1A8A!5. 1)

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    "hase II3 Feed Formulation