Lumina Fluorescence Spectrometer Installation Guide 100510…

Lumina Fluorescence SpectrometerInstallation Guide

The information in this publication is provided for reference only. All information contained in this publication is believed to be correct and complete. Thermo Fisher Scientific shall not be liable for errors contained herein not for incidental or consequential damage in connection with the furnishing, performance or use of this material. All product specifications, as well as the information contained in this publication, are subject to change without notice. This publication may contain or reference information and products protected by copyrights or patents, and it does not convey any license under our patent rights, nor the rights of others. We do not assume any liability arising out of any infringements of patents or other rights of third parties. We make no warranty of any kind with regard to this material, including but not limited to the implied warranties of merchantability and fitness for a particular purpose. Customers are ultimately responsible for validation of their systems. © 2008-2010 Thermo Fisher Scientific Inc. All rights reserved. No part of this publication may be stored in a retrieval system, transmitted, or reproduced in any way, including but not limited to photocopy, photograph, magnetic or other record, without our prior written permission. For Technical Support, please contact: Thermo Fisher Scientific 5225 Verona Road Madison, WI 53711-4495 U.S.A. Telephone: 1 800 532 4752 E-mail: [email protected] World Wide Web: http://www.thermo.com/spectroscopy For International Support, please contact: Thermo Fisher Scientific Telephone: +1 608 273 5017 E-mail: [email protected] World Wide Web: http://www.thermo.com/spectroscopy Microsoft, Windows, Windows NT, Windows XP and/or other Microsoft products referenced herein are either trademarks or registered trademarks of Microsoft Corporation in the United States and/or other countries. Pentium is a registered trademark of Intel Corporation in the U.S. and other countries. Adobe and Acrobat are either trademarks or registered trademarks of Adobe Systems, Incorporated in the United States and/or other countries. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

Contents

I. Introduction ....................................................................................1

I-1. Safety......................................................................................1

II. Installation ......................................................................................8

II-1. Pre-Installation......................................................................8

II-1-1. Instruction ..................................................................... 8

II-1-2. Pre-Installation Checklist ............................................... 8

II-1-3. Environmental Conditions ............................................. 8

II-1-4. Electrical Specifications ................................................ 10

II-1-5. Equipment on-site ........................................................ 11

II-1-6. Environmental Requirements ....................................... 13

III. Lumina Installation...................................................................14

IV. Luminous Software Installation..................................................15

IV-1. How to Install Luminous software .......................................15

IV-2. Re-Install of Luminous software...........................................18

V. Operating Guide ........................................................................19

VI. Diagnostic Software ...................................................................24

VII. Maintenance..............................................................................38

VII-1. Xenon Lamp Replacement ...................................................38

VII-1-1. Xenon Lamp Replacement Procedure ........................... 40

VII-2. Fuse Replacement ................................................................45

VII-2-1. Xenon Lamp Replacement Procedure ........................... 46

VII-2-2. Replacement of a fuse in the noise filter ........................ 47

VII-3. Trouble Shooting ................................................................48

Appendix 1 ........................................................................................51

1. Lumina Fluorescence Spectrometer .......................................51

2. Basic Theory of Fluorescence Spectrometry ...........................51

Appendix 2 ........................................................................................54

1. Specification..........................................................................54

Appendix 3 ........................................................................................55

1. Optical System ......................................................................55

1 Lumina Fluorescence Spectrometer Installation Guide Thermo Scientific

I. Introduction

I-1. Safety

The following safety symbols are used on this product.

Warning Description

Always refer to the system manual when working near

locations at which the alert mark shown on the left is

attached. If the operation, etc., is performed without

heeding the advice in the System manual, there is a risk

of personal injury. In addition, the equipment

performance may be reduced. Moreover, this alert mark

is sometimes used with other marks and descriptions

indicating other dangers.

Warning

When supplying power to this equipment, connect the

accessory 3-pin power cord to a 3-pin grounded power

outlet. If a grounded 3-pin outlet is not available, use a

conversion adapter and ground the green wire. If power

is supplied without grounding the equipment, there is a

risk of receiving a fatal electric shock and equipment

damage.

‘On’ (power)

To indicate connection to the mains, at least for mains

switches or their positions, and all those cases where

safety is involves

‘OFF’ (power)

To indicate disconnection from the mains, at least for mains switches or their positions, and all those case where safety is involves.

Thermo Scientific Lumina Fluorescence Spectrometer Installation Guide 2

Repair

Warning

The user cannot repair this equipment.

DO NOT attempt to open the case or to disassemble

internal parts.

Only Thermo Scientific trained service personnel or staff

from your sales representative with knowledge of

electrical fire and shock hazard should service this

equipment.

There are high-voltage parts in this equipment

presenting a risk of severe injury or fatal electric shock

to untrained personnel.

In addition, there is a risk of damage to precision parts.

Falling Over

Caution

This equipment should be used in the correct position. If

the equipment is turned on its side, etc., it will be

unstable and may be damaged if it falls over as a result

of receiving a slight mechanical shock.

Changing Fuse

Warning

Before changing the fuses, ALWAYS remove the power

cord from the power-outlet and replace the blown fuses.

ALWAYS use new fuses of the type and rating specified

on the fuse marking on the rear panel cabinet.

T2AL indicates a time-large fuse. There is risk of

receiving a fatal electric shock if the fuses are replaced

with the power cord connected.

Cleaning

Warning

Keep the equipment free of dust.

Clean the power line regularly; if dust accumulates

around the power pins, there is a risk of fire.

Keep the equipment clean so that the ventilation holds

are not obstructed. If the ventilation is obstructed, the

system may overheat and catch fire.

Periodically clean your equipment's case using a damp

cloth.

Do not use abrasives, cleaning solvents or strong

detergents, as they may damage the finish or affect the

reliability of the structural components.

If this equipment is used in a manner not specified by

this manual, the protection provided by the equipment

may be impaired.


WEEE Compliance

This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol:

Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product. See www.thermo.com/WEEERoHS for further information on Thermo Fisher Scientific’s compliance with these Directives and the recyclers in your country.

Conformité DEEE

Ce produit est conforme à la directive européenne (2002/96/EC) nommée Déchets d'Equipements Electriques et Electroniques (DEEE). Il est marqué par le symbole suivant:

Thermo Fisher Scientific s'est associé à une ou plusieurs sociétés de recyclage dans chaque état membre de l’Union Européenne. Ce produit devra être collecté ou recyclé par celles-ci. Toutes les informations sur la conformité des produits Thermo Fisher Scientific à ces directives, les sociétés de recyclage de votre pays et les informations sur les produits Thermo Fisher Scientific qui peuvent aider à la détection des substances sujettes à la directive RoHS sont disponibles sur www.thermo.com/WEEERoHS.


WEEE Konformität

Dieses Produkt muss die EU Waste Electrical & Electronic Equipment (WEEE) Richtlinie 2002/96/EC erfüllen. Das Produkt ist durch folgendes Symbol gekennzeichnet:

Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs-/Entsorgungsfirmen in allen EU-Mitgliedsstaaten getroffen, damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann. Mehr Information über die Einhaltung dieser Anweisungen durch Thermo Fisher Scientific, über die Verwerter, und weitere Hinweise, die nützlich sind, um die Produkte zu identifizieren, die unter diese RoHS Anweisung fallen, finden sie unter www.thermo.com/WEEERoHS.

Conformità RAEE

Si richiede che questo prodotto soddisfi la direttiva dell'Unione Europea sui rifiuti di apparecchiature elettriche ed elettroniche (RAEE) 2002/96/CE. È contrassegnato col seguente simbolo:

Thermo Fisher Scientific ha concluso accordi con una o più società di riciclo/smaltimento in ciascuno Stato membro dell'UE, e questo prodotto va smaltito o riciclato attraverso tali società. Ulteriori informazioni sulla conformità di Thermo Fisher Scientific a queste Direttive, sulle società addette al riciclo nel proprio Paese e informazioni sui prodotti Thermo Fisher Scientific che potrebbero essere utili nel rilevamento di sostanze soggette alla Direttiva RoHS sono disponibili all'indirizzo www.thermo.com/WEEERoHSS.


Conformidad WEEE

Este producto debe cumplir la Directiva 2002/96/CE de la Unión Europea sobre Desechos eléctricos y de equipos electrónicos (WEEE). Está marcado con el símbolo siguiente:

Thermo Fisher Scientific ha contactado con una o varias empresas de reciclaje o eliminación de residuos en cada país miembro de la Unión Europea (EU) y dichas empresas deben eliminar o reciclar este producto. Consulte www.thermo.com/WEEERoHS para obtener más información sobre el cumplimiento de estas directivas por parte de Thermo Fisher Scientific y sobre las empresas de reciclaje de su país.


FCC Verification

This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference in a residential installation This equipment generates uses and can radiate radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interference to radio communications, However, there is no guarantee that interference will not occur in a particular installation. If this equipment does cause harmful interference to radio or television reception, which can be determined by turning the equipment off and on, the user is encouraged to try to correct the interference by one or more of the following measures:

- Reorient or relocate the receiving antenna. - Increase the separation between the equipment and receiver. - Connect the equipment into an outlet on a circuit different from that to which the receiver is connected. - Consult the dealer or an experienced radio/TV technician for help.


Equipment Cinfiguration

The table shows the configuration of the Lumina.

Item Name Qty. Remark

Main Instrument

Lumina Fluorescence Spectrometer 1

Accessories Power Cord 1 2.0 m

Interface Cable (RS-232) 1 2.0 m

Converter (RS-232 to USB)

Luminous Software User Guide 1

Lumina Installation Guide 1

Luminous Software CD 1

Spare Fuse (AC 250V, T5AL) 2

Spare Fuse (AC 250V, T2AL) 1


II. Installation

II-1. Pre-Installation

II-1-1. Instruction

Lumina fluorescence spectrometer is designed to provide a

complete analysis system for fluorescence, phosphorescence

and chemiluminescence measurements.

All instruments will be tested and proven to the performance

before the shipment

II-1-2. Pre-Installation Checklist

Preparation Done

The installation place should be prepared / checked. □

The electric power is available / stable. □

The system and accessories should be unpacked on site. □

Proper computer system should be prepared. □

Windows operating system should be prepared. □

Quartz cell for fluorescence is available. □

Operation training is required. □

II-1-3. Environmental Conditions

You are responsible for providing an acceptable operating

environment. Attention paid to the operating environment

well ensures the continued high performance of your Lumina

fluorescence instrument. The instrument warranty will be


made void if the equipment is operated in sub-standard

conditions.

Humidity

The relative humidity of the operating environment should be

lower than Maximum 75% with no condensation. Operating

the Lumina fluorescence in very low humidity may result in

the accumulation and discharge of static electricity that

shortens the life of electronic components. Operating at high

humidity will produce condensation and result in short

circuits. Thermo Scientific recommends that your laboratory

be equipped with a temperature / humidity monitor. This will

ensure that your laboratory is always in conformance with the

temperature and humidity specifications.

Temperature

Air conditioning is recommended. The room should be

temperature controlled if your analyses are particularly

sensitive. For optimum analytical performance it is

recommended that the ambient temperature of the laboratory

be between 15-35 and be held constant to within +/℃ -2%

throughout the entire working day. As laboratory temperature

increases, system reliability decreases and all electronic

components generate heat while operating. This heat must be

dissipated to the surrounding air so that the components are

operating correctly.

Vibration

Ensure that lab benches are free from vibration. If possible,

any equipment generating vibration during the operation

should be installed on the floor rather than alongside the

system on the workbench.


Work bench

The workbench must be stable and durable enough to support

the total weight of equipment. The worktops should be large

enough to permit a free circulation of air around each unit in

the system. Remember to provide space for the computer,

monitor and printer. To avoid damage through spillage of the

samples being analyzed, the worktops should be covered with a

material which is corrosion resistant and impervious to liquids.

** Lumina dimensions are 620 mm (W) x 710 mm (L) x 300 mm (H)

II-1-4. Electrical Specifications

In view of the electronic components in the Lumina system, it

should be positioned well away from any instruments

emitting an electromagnetic field and any environment that

may cause vibration.

Power Supply

All power supplies must be single phase AC voltage, three wire

system (active, neutral, ground) and should be terminated at

an appropriate power outlet and/or regulations imposed by

local authorities responsible for the supply of electrical energy

to the workplace.

A grounded AC power supply must be used. The Lumina

accepts a dual voltage of either 110 or 220V at 50㎐ or 60㎐

and requires 300 W power including computer and printer.

Good electrical grounding is essential to avoid potentially

hazardous shock hazards. 3-wire outlet with ground

connection must be provided for the instrument. Make


certain that power outlets are grounded at the grounding pin.

Avoid using power supplies from a source that may be subject

to electrical or RF interference from other services (large

electric motors, elevators, and welders for example).

Two fuses are used in the Lumina fluorescence spectrometer;

Fuse A: AC 250V, T2AL

Fuse B: AC 250V, T5AL

II-1-5. Equipment on-site

Insurance

As the carrier’s liability ceases when equipment is delivered,

Thermo Scientific recommends that the instrument owner arrange

a separate insurance to cover transportation from the delivery point

to the installation site. The delivery point will vary according to the

carrier, the shipping method and in some cases the terms of sale.

Some carriers will deliver only to their own distribution centers,

who then will deliver to the actual installation site.

Inspection for transit damage

Transit damage can be obvious or concealed and in either case will

be admitted by the carrier only if it is reported within the terms of

the carrier’s agreement. For any claims against damage in transit,

the following general rules apply;

- Before accepting delivery, you must inspect the packages for signs

of obvious damage. The nature of any obvious damage must be

noted on the carrier’s waybill, which then must be countersigned

by a representative of the carrier


- Within the time limit stated in the terms and conditions of

carriage, a further inspection must be made for concealed

damage. If any damage is found at this stage, the carrier must be

modified in writing. You must retain all packing material for

subsequent inspection by a representative of the carrier.

- A copy of any damage report must be forwarded to Thermo

Scientific or Sales representative.

- ‘SHOCK WATCH vibration indicator label that helps you to

aware of the damage level are attached on Packing Boxes. If the

indicator color is changed as red color, it means the packing box

could be impacted/damaged by the physical shock externally.

If it is happened, contact Thermo Scientific or sales

representative immediately to hear the next procedures.

(* Take a picture to provide the insurance company for the

evidence if it is Available)

.


II-1-6. Environmental Requirements

Requirement Description

Indoor use only Altitude up to 2000m

Pollution degree 2

Dimensions 620 (W) x 710 (L) x 300 (H) mm

Electrical supply AC 100-240V, 50/60Hz, 240W

Operating Temperature 15 - 35℃

Operating humidity Maximum 75% relative, non-condensing

Main Supply Voltage Fluctuation

± 10%

Ventilation Air Cooling System (DC FAN)

II-1-7. Computer Requirements

Requirement Description

Hardware

IBM Compatible PC (Pentium 133 MHz [or Greater] Processor)

At least 64 MB RAM

CD-ROM disk drive

HDD

RS-232 port

Mouse & Keyboard

Operating System

Windows 98, Windows ME, Windows 2000 or Windows XP

Output Device Printer


III. Lumina Installation

1. Carefully remove the instrument and all of its components

from the shipping container.

2. Verify that all of the components received match the

packing list.

3. Verify that all system components required for installation

are present, this, must include:

□ Lumina Fluorescence Spectrometer Interface cable□ (RS-232) Power Cord □ □ Luminous Software CD Computer systems □

4. Place the instrument in a location that is compatible with the

required environmental conditions for operation. Connect the

power cord to the plug; the power plug must be grounded.

Warning

The electric power could be dangerous to the body. Before

connecting the power cord, verify that the main power

switch is off.


IV. Luminous Software Installation

IV-1. How to Install Luminous software

1. Insert Luminous Software installation CD into the CD-ROM

drive. InstallShield® Wizard starts automatically.

2. If InstallShield Wizard does not start automatically, click My

Computer → E Drive and then, double click Setup.exe and click

Next.

3. When the following dialog box appears, enter customer

information, if necessary, and click Next.


4. The destination folder and user information will be shown. Click

Install. Installation will begin.


5. After the set up is completed successfully, click Finish.

6. Find the newly created ‘Luminous Software’ folder on the

Desktop.


IV-2. Re-Install of Luminous software

1. To re-install the Luminous program, previously installed.

2. Luminous program must be removed. Select Start →

Control Panel Add/Remove Programs.→

3. Select the Luminous. Then click Remove and select Yes.

4. Luminous program will be removed automatically.

Thereafter, reinstall the Luminous software.


V. Operating Guide

To start the Luminous Software, follow up the procedures

below.

1. Turn on the main power switch on the rear panel of the

system.

2. Turn on the sub main power switch at the bottom right

side of the front panel of the system.

3. Push the Light Source button (Red) to turn on light firstly

and push the Control Board button (White) to turn on

the main board.

Caution!

a. It is important to keep up the order during turning

powers on:

#1 Main P/S #2 Light Source #3 Control Board

b. If the control board is powered on at first , it could cause

damage to the electric board by the high voltage/current

when the light source is on later.

c. To turn off the system, you need to follow the procedures

3 2 1


in reverse order against the start up. (#3 Control Board->

#2 Light Source -> #1 Main P/S)

d. Before turning off the main power switch, you need to

wait for over 10 minutes at least to protect the lifetime of

lamp after turned off the light source switch.

4. Check whether all three LED lights at the left side of the

front panel of the system are lit on. If it is ready, warm up

the system for about 20-30 minutes.

5. Double click / open the Luminous Software folder on

Desktop.

6. Double click Wave Scan in the Luminous Software

folder, then the following windows will be shown.


7. Click NEW in the toolbar.

8. Enter a file name in the New window and click Save.


9. The Create New Experiment window is displayed. Enter title,

customer and User ID if it is needed and then click OK.

10. The main window will be displayed.

11. Set up parameters in the measurement setup window

depending on the experiment conditions. Refer to the

Luminous software user guide for more details.(Chapter

IV. Measurement Mode)


12. Place the sample in the sample cell holder.

13. Click the Start button.

14. The result spectrum and data will be displayed as below.

15. Data can be printed out by clicking the Report View

icon. See the Lumonous Software User Guide for

more details.


VI. Diagnostic Software

Diagnostics software is to check if each component of

instrument works correctly.

Teflon Block is needed for diagnostics.

1. Turn on the main power switch on the rear panel of the system.

2. Turn on the sub main power switch at the bottom right side of

the front panel of the system

3. Push the Light Source button (Red) to turn on light firstly

and push the Control Board button (White) to turn on the

main board.

Caution!

a. It is important to keep up the order during turning powers

on:

#1 Main P/S #2 Light Source #3 Control Board

b. If the control board is powered on at first , it could cause

damage to the electric board by the high voltage/current

when the light source is on later.

1 23


c. To turn off the system, you need to follow the procedures

in reverse order against the start up. (#3 Control Board ->

#2 Light Source -> #1 Main P/S)

d. Before turning off the main power switch, you need to

wait for over 10 minutes at least to protect the lifetime of

lamp after turned off the light source switch.

4. Check whether all three LED lights at the left side of the

front panel of the system are lit on. If it is ready, warm up

the system for about 20-30 minutes.

5. Double click the Diagnostics icon in the Luminous Software

folder on Desktop.

6. Click OK after ‘Reading serial number: OK window’ is

displayed.


7. You can check the serial number of instrument

8. Select the items which you want to inspect and enter the proper

repeat numbers in Repeat Number boxes on the Main Controller

Test panel. For the initial inspection, all items are recommended

to be checked and the proper repeat number is ‘2-5’.

9. Click Start.


10. You will see the following message.

11. Place the Teflon block into the cell holder, and then click

OK.

12. The total diagnostic progress status and the progress status of

each component will be shown.


13. If the diagnostic passes the test, then the following message will

be shown.

Status of total diagnostic progress

Checked items will be tested.

Progress status of each component


14. If each procedure has no problem, the test result will be

displayed as ‘pass’. If any procedure has a problem, ‘fail’

message will be displayed and the system will be stopped.

15. If the result is failed, the following message will be shown. If

you did not recalibrate the wavelength in Alignment.exe,

recalibrate both monochromators using Alignment.exe


16. Click Print to print out the result of diagnostics.

17. Click Help to search the items to be defined.


18. Diagnostic Error Chart.

Error Cause Corrective action

Poor cable connection Check RS-232 Cable connection

Computer COM port failed.Check Computer COM port and connect with other computer for the cross-checking

Instrument COM port failed.

Connect with other computer for the cross-checking

Main Board Failed

Bad Main controller in the board

Replace Main board.

Xenon lamp is not turned on.

Run the diagnostic software

Wavelength is shifted. Recalibrate the wavelength using Alignment.exe

Excitation Slit positions are not correct.

Open the upper cover and check the Excitation Slit positions. If it is misaligned, Recalibrate the slit positions using Alignment software.

Excitation Filter positions are not correct.

Open the upper cover and check the Excitation Filter positions. If it is misaligned, Recalibrate the Filter positions using Alignment software.

Excitation Slit Failed

Excitation Monochromator is not correct.

Open the upper cover and check the Excitation monchromator position. If it is misaligned, Recalibrate the monochromator position using Alignment software. .



Detector cannot receive the signal.

Check the cable connection or Replace PD board.

Excitation Slit Failed Start position is different every time or Slit Wheel rotates continuously.

Limit switch could be defected. Replace the limit switch.


Run the diagnostic software.





Excitation Monochromator is not correct.




Excitation Filter Failed

Start position is different every time or Slit Wheel rotates continuously.






Excitation monochromator is not correct.








Excitation

Monochromator Failed







Teflon Block is not installed into the cell holder.

Put the Teflon Block into cell holder.

Emission Slit positions are not correct.

Open the upper cover and check the Emission Slit positions. If it is misaligned, Recalibrate the slit positions using Alignment software.

Emission Filter positions are not correct.

Open the upper cover and check the Emission Filter positions. If it is misaligned, Recalibrate the Filter positions using Alignment software.

Emission Monochromator is not correct.

Open the upper cover and check the Emission monchromator position. If it is misaligned, Recalibrate the monochromator position using Alignment software. .




Check the PMT detector and cable connection.

Emission Slit Failed

Shutters are not opened. Refer to ‘shutter check is failed’.




Run the diagnostic software

Teflon Block is not installed into cell holder.







Open the upper cover and check the Emission monchromator position. If it is misaligned, Recalibrate the monochromator position using Alignment software.


Limit switch could be defected. Replace the limit switch



Emission Filter Failed

Shutters are not opened Refer to ‘shutter check is failed’.





Teflon Block is not installed into cell holder.



Open the upper cover and check the Emission monchromator position. If it is misaligned, Recalibrate the monochromator position using Alignment software.









Emission Monochromator

Failed

Shutters are not opened. Refer to ‘shutter check is failed’.



Excitation Shutter Check Failed

Shutter is not opened.

Check the solenoid valve is working properly, cable connection and for Input voltage(12V) with test meter.

Emission Shutter Check Failed

Shutter is not opened.

Check the solenoid valve is working properly, cable connection and for Input voltage(12V) with test meter.


VII. Maintenance

VII-1. Xenon Lamp Replacement

The Xenon lamp for replacement is packed in the

manufacturer’s box and must be installed in the lamp housing

correctly. Read all the precautions before attempting to replace

the Xenon lamp.

Caution

A. Xenon arc lamp has an explosion hazard. Make sure the

system’s power is off and all AC power is disconnected from

the system. Read and follow the cautions presented below.

B. Xenon arc lamp has an explosion hazard. Wear goggles and

protective clothing when opening the lamp housing and

when handling the lamp.

C. The lamp will remain extremely hot for approximately one-

half hour after it has been turned off. Do not touch the

lamp or the metal unit until the lamp has cooled.

D. Never look directly at the xenon arc or its reflection.

Intense radiation can permanently damage eyes.

E. Do not touch the focusing lens, back scatter mirror, or the

surface of the lamp. Fingerprints will be burned onto these

surfaces when the lamp is ignited.


Xenon Lamp Replacement Flow Chart

1) Start

2) Turn off the power of the instrument.

3) Remove white screw caps (total 10) located around on the

upper case.

4) Remove Phillips Head screws using a Phillips screwdriver.

5) Remove Phillips head screws (4 ea) located on the Sample Cell

Assay part using a Phillips screwdriver.

6) Separate the Sample Cell Assay part and lift the upper case.

7) Get rid of socket screws (4 ea) using a hexa socket screwdriver.

8) Separate the lamp connector from the lamp power module

and push the hap and pull out the connector.

9) Remove the lamp mounting module from the lamp housing.

10) Remove the screws (2 ea) located on the edges of the lamp.

11) Unfasten the socket screws (2ea) located on the (+) side

of the Teflon bracket.

12) Unpack a new Xenon lamp.

13) Install the lamp in the correct polarity.

14) Fasten the socket screw (2ea) located in (+) direction

Teflon bracket. Put the changed lamp module back in the

lamp housing.

15) Finally, connect the lamp cable to the power supply cable.

16) End


VII-1-1. Xenon Lamp Replacement Procedure

1. Check the main power off (If the system and lamp are on,

it requires enough cooling time of lamp over 10 minutes

after turning off the control board and lamp. Then, turn

off the sub main power switch at the bottom right side of

the front panel and the main power on the rear panel)

2. Remove white screw caps (total 10 ea) located around on

the upper case.

3. Remove Phillips head screws using a Phillips screwdriver.


4. Remove Phillips head screws (4ea) located on the

compartment using a Phillips screwdriver.

5. Separate the sample compartment and lift the upper case.

6. Get rid of socket screws (4ea) using a hex socket screwdriver.

7. Separate the lamp connector from the lamp power

module and push the hap and pull out the connector.


8. Remove the lamp mounting module from the lamp housing.

9. Remove the screws (2ea) located on the edges of the lamp.

10. Unfasten the socket screws (2ea) located on the (+) side of the

Teflon bracket.


11. Unpack a new Xenon lamp.

12. Install the lamp in the correct polarity.

Caution:

Install the lamp in the correct polarity. Otherwise it could

cause burst, overheating of the lamp house or short lamp life.

(Upper side is plus polarity)

13. Do not use any tools to tighten nuts when fixing the

lamp, use hands only. Otherwise it could cause breakage.

14. Fix the lamp and its lead wire firmly to the terminal.


15. Fasten the socket screw (2ea) located in (+) direction

Teflon bracket. Put the changed lamp module back in the

lamp housing.

16. Finally, connect the lamp cable to the power supply cable.


VII-2. Fuse Replacement

Flow Chart of the Fuse Replacement in the fuse holder

1) Start


3) Loosen the fuse holder on the rear side of the instrument.

4) Pull out the fuse (AC 250V. T2AL) and exchange it with

a new one.

5) Put the fuse holder back and tighten.

6) End

Flow Chart of the Fuse Replacement in the noise filter

1) Start


3) Pull out the fuse box on the rear side of the instrument.

4) Replace the fuse (AC 250V, T5AL) with a new one.

5) Put the fuse box back as it was.

6) End


VII-2-1. Xenon Lamp Replacement Procedure

1. Check the main power off (If the system and lamp are on, it

requires enough cooling time of lamp over 10 minutes after

turning off the control board and lamp. Then, turn off the sub

main power switch at the bottom right side of the front panel

and the main power on the rear panel)

2. Loosen the fuse holder by turning it clockwise on the rear side

of the instrument, using (-) driver.

3. Pull out the fuse (AC 250V, T2AL) and exchange it with a new

one.

4. Put the fuse holder back and tighten.

Caution: Should use the fuse of rated capacity (AC 250V, T2AL).


VII-2-2. Replacement of a fuse in the noise filter

1. Check the main power off (If the system and lamp are on, it

requires enough cooling time of lamp over 10 minutes after

turning off the control board and lamp. Then, turn off the sub

main power switch at the bottom right side of the front panel

and the main power on the rear panel)

2. Pull out the fuse box on the rear side of the instrument as below.

3. Replace the fuse (AC 250V, T5AL) with a new one.

Caution: Should use the fuse of rated capacity (AC 250V, T5AL).

4. Put the fuse box back as it was.


VII-3. Trouble Shooting

Lumina Fluorescence spectrometer system is designed to operate

reliably and predictably. If the instrument has a problem, take

measures by referring to the table below. When a symptom in

question is not found in the table, contact Thermo Scientific or sales

representative.

Problem Possible Cause Solution

Power cable is not securely connected.

Securely insert the plugs at both ends of the cable into the inlet on the instrument and the outlet at the site.

Instrument is not powered though the power switch is in the ON position

Power fuse is blown. Replace the fuse with a new one.

Shutter is in the closed position, or the slit on the emission side is in the ‘Close’ status.

Using software, open the shutter or the slit.

Slits are not opened to the proper width.

Contact Thermo Scientific or sales representative.

Lamp is not turned on.

Refer to ‘Lamp is not turned on’ problem below.

Monochromator is mis-calibrated.

Check and recalibrate monochromators. If the problem is not solved after the monochromator recalibration, Contact Thermo Scientific or sales representative.

Light is not reaching the sample

Accessory is not in the right position.

Relocate the position.



Wiring is disconnected.

Check the connection of the high-voltage lead on the positive (+) electrode side of the lamp. Before opening the cover of the lamp house, be sure to disconnect the power cable from outlet. Inadvertently powering ON of the instrument with the cover open is extremely dangerous.

Lamp is not turned

on

Lamp is still hot. Allow the lamp to cool down for about ten minutes.

Xenon lamp is not stably lit.

After turning on the power of the instrument, wait for at least 30 minutes until the Xenon lamp is stably lit.

Xenon lamp is aged. Replace the Xenon lamp with a new one

S/N ratio does not

satisfy the

guaranteed value

Distilled water is contaminated.

Contaminated distilled water or dirty cell will result in the increased background noise owing to scattered light or impurity-induced fluorescence, eventually decreasing the S/N ratio. Rinse the cell thoroughly. Use clean distilled water only.



The Xenon lamp is misaligned.

Position lamp correctly.

Wrong acquisition parameters.

Correctly specify the wavelength, slit width , filter etc.

Shutter is in the closed position, or the slit on the emission side is in ‘Close’ status.

Using software, open the shutter or slit.

Lamp is not turned on. Refer to ‘Lamp is not turned on’ symptom above.

Signal does not

come out

Data is saturated. Reduce slit setting or adjust PMT voltage setting properly.

Lamp is not turned on. Run the diagnostic software.

Abnormal spectra

results Light leaks.

Check dark value to determine.

Hardware Initializing

error

IR sensors in a monochromator, a slit and a filter could be broken.

Replace the IR sensor.


Appendix 1

1. Lumina Fluorescence Spectrometer

The rapid growth & convergence of high technology has created

the opportunity for a technologically advanced Fluorescence

Spectrometer, Thermo Scientific Lumina. The Lumina efficiently

provides a complete analytical environment with rapid

measurements and highly stable results. Thereby it maximizes

laboratory efficiency in various fields such as pharmaceutical,

environmental and life sciences, etc.

2. Basic Theory of Fluorescence Spectrometry

Fluorescence Spectrometry, also known as fluorometry or

spectrofluorometry, is a type of electromagnetic spectroscopy which

analyzes fluorescence from sample. It involves using a beam of light,

usually ultraviolet and visible light, which excites the electrons in

molecules of certain compounds and causes them to emit light of

lower energy, typically, but not necessarily, visible light. A

complementary technique is absorption spectroscopy.

Molecules have various states referred to as energy levels.

Fluorescence spectroscopy is primarily concerned with electronic

and vibrational states. Generally, the species being examined will

have a ground electronic state of low energy and an excited

electronic state of higher energy. Within each of these electronic

states are various vibrational states.


In fluorescence spectroscopy, the species is first excited, by

absorbing a photon, from its ground electronic state to one of the

various vibrational states in the excited electronic state. Collisions

with other molecules cause the excited molecule to lose vibrational

energy until it reaches the lowest vibrational state of the excited

electronic state.

The molecule then drops down to one of the various vibrational

levels of the ground electronic state again, emitting a photon in the

process. As molecules may drop down into any of several

vibrational levels in the ground state, the emitted photons will have

different energies, and thus different frequencies. Therefore, by

analyzing the different frequencies of light emitted in fluorescent

spectroscopy, along with their relative intensities, the structure of

the different vibrational levels can be determined.

In a typical experiment, the different frequencies of fluorescent


light emitted by a sample are measured, holding the excitation light

at a constant wavelength. This is called an emission spectrum. An

excitation spectrum is measured by recording a number of emission

spectra using different wavelengths of excitation light.


Appendix 2

1. Specification

Light Source 150 W CW Xenon-arc lamp

Photodiode for the reference Detector

PMT for Excitation and Emission Spectrum

Wavelength Range 190 - 900 nm for Excitation and Emission

Better than 1000 : 1 (peak to peak),

Better than 4000 : 1 (RMS)

Slit width : 10 nm, PMT Voltage : 700 V;

Integration Time : 200 ms,

Sensitivity

Scan Speed : 1000 nm/min

1200 groove/mm, 250 nm blazed for Monochromator

Excitation, 400 nm blazed for Emission.

Wavelength Scan Speed 1 - 6000 nm/min

Wavelength Slew Speed 20,000 nm/min

Minimum Resolution 0.5 nm

Wavelength Accuracy ± 0.5 nm

Wavelength Reproducibility

± 0.2 nm

Slit width 0.5, 1, 2.5, 5, 10, 20 nm

Dimensions (mm) 620 (W) x 710 (L) x 300 (H)

Weight 51.0 Kg

Interface RS-232

Power Requirement AC100 - 240V, 50/60 Hz, 240W


Appendix 3

1. Optical System

BR : Reflection mirror, CL: Collimation lens, FL: Focusing Lens, F:

Filter, S: Slit, M: Mirror, G: Grating, BS: Beam splitter, PD:

Photodiode, UVW: UV grade Window, PMT:

Photomultiplier tube

Lumina uses CW Xe-lamp as a source. The forward light is

collimated by Collimation lens(CL1) and the backward light is

collected/reflected by Reflection mirror(BR) and then, it will be also

collimated by Collimation lens(CL1). The collimated beam will be

focused by Focusing lens(FL1) and go through Excitation slit(SEX)

which is in Monochromator. The other light could be filtered using

Excitation Filter(FEX) except the specific wavelength if it is

necessary. After slit, the incident light will be reflected by Concave


Mirror(MEX1) which is 190mm far from slit and then, will be

dispersed by Excitation Grating. The dispersed light is reflected

again by Concave Mirror (MEX2) and go to the Excitation exit slit.

Also, the wavelength we want can be pass through the Excitation

exit slit by controlling the incident angle and reflective angle of

Grating (GEX). The monochromated light after passing through

the exit slit is reflected by Flat Mirror (M2) and again reflected by

Concave Mirror (M1) then, will be focused. The focused light goes

through the beamsplitter(BS) and the 2% portion of light goes to

Photodiode(PD) after reflected and other 98% pass through the

sample.

Using UV grade Window(UVW), the inner optical parts will be

protected from the outer air and dust. The sample absorbed by the

Excitation light will emit the fluorescence and the emitted

fluorescence will be collimated after passing through the collimation

lens(CL2) and focused by Focusing lens(FL2) then, goes to the

monochromator through the Emission entrance slit. The unwanted

wavelength could be filtered by the Optical filter (FEM) before

going to the monochromator per request. The incident light is

reflected by Mirror(MEM1) and monochromated by

Grating(GEM) then, the selected light will be passed through the

exit slit of Emission monochromator. This selected light goes to

PMT and the electrical signal will be read out after processing.

Lumina Fluorescence Spectrometer Installation Guide 100510…

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