LT-2/ LT-3/ LT-4

CERTIFICATE PROGRAMME INLABORATORY TECHNIQUES (CPLT)

Term-End ExaminationDecember, 2009

LT- 2 : Laboratory Techniques in BiologyLT- 3 : Laboratory Techniques in ChemistryLT- 4 : Laboratory Techniques in Physics

03910

Time Allowed : 3 Hours Maximum Marks : 150

Note :This question paper contains three parts, one each for LT-2, LT-3 and LT-4 courses.Maximum time allowed for each part is 1 hour.

Students are required to answer all the three parts in three separate answer books. Write your

Enrolment number, Course code and Course title clearly on each of the three answer books.

(iii) Marks are indicated against each question.

LT-2 : Laboratory Techniques in BiologyTime : 1 hour Maximum Marks : 50

1. (a) Fill in the blanks :(i) Collection of preserved and labelled specimens of plants are stored in a

The vessels designed for staining of slides are known as jars.The components of a suspension can be concentrated or separated by usinga

(iv) A simple method for study of chromosomes in a cell is technique.

(b) Differentiate between the following giving two differences of each :Animal house and Green house.Progressive staining and Regressive staining.

4

4

LT-2/ LT-3/LT-4

1 P.T.O.

State whether the following statements are true or false : 4

(i) Gram's stain is used for staining bacteria.

A kymograph apparatus is used to measure / record microcirculation.

Earthworms and leeches are hermaphrodite.

Scalpel is an instrument used for digging.

Name any two fixtures that are used in a biology laboratory. 1

(e) Name the instrument used for : 2

(i)

Cutting fine sections.

(ii) Measuring density of colour of a solution.

2. Attempt any five parts. Limit your answer to 50-60 words :

How would you dry a plant specimen without artificial heat ? Givedisadvantages of this method.

What is meant by sterilization ? Enumerate four basic methods ofand describe any one of them.

How would you label the permanent slides given to you for storagelaboratory?

How would you maintain a light microscope in your laboratory ?

Describe correct techniques to collect plant and animal materials.

Explain the steps involved in making a temporary slide.

any two 5

sterilization 5

in a biology 5

5

55

3. Attempt any one part. Limit your answer to 100-120 words :

With the help of a labelled diagram describe the working of a distillation unit. 10What is distilled water ? Give two precautions to be taken.

What kind of arrangement would you make to accommodate the necessary 10features of a biological laboratory ?

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LT-2/LT-3/LT-4 2

LT-3 : Laboratory Techniques in Chemistry

Time : 1 hour Maximum Marks : 50

Note : Answer all the questions.

Answer all the questions : 1x10=10

./Ethanol (b.p. 78°C) should be distilled using a / an condenser.(air, water).An impurity will the boiling point of a liquid. (lower, raise)In a mixture of water and carbon disulphide, water will form the layer. (lower, upper)Aquaregia is a mixture in 3:1 ratio of conc. and conc. (HC1, HNO3, H2SO4)Small pieces of boiling chips are added to the liquid under distillation to prevent . (bumping, decomposition)The Rf value of a substance can be . (0.50, 1.50)Column chromatography involves the principle of . (absorption,adsorption)A solution of sodium carbonate will be (acidic, alkaline)Droppers and pipettes are kept in racks. (horizontally, vertically)A desiccator is used for substances. (preserving, drying)

(k) Softening temperature of soda glass is than that of borosilicate glass.(lower, higher)

(1) Methanol is a solvent. (polar, non-polar)

2. (a) Describe with the help of a diagram any one of the following :Siwoloboff's method for boiling point determination.Simple distillation of alcohol (b.p. 78°C).

(b) Name three fuel gases used for glass blowing. Name the gas : 21/2which has the highest calorific value andwhich can provide the highest temperature.

(c) 40 cm3 of an aqueous copper (II) sulphate solution contains 10 g of CuSO4 .5H20. 21/2Express the concentration of this solution in % w/v.

3. Attempt any two parts from (a), (b) and (c).(a) Give the method of preparation of the following :

15% w/v sodium hydroxide solution. 2

Chlorine water. 1(iii) A solution of 0.5N Oxalic acid (Eq. Wt. 63) 2

1. Fill in the blanks with appropriate words chosen from those given in the bracketsalongside. Answer any ten of the following :

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LT-2/ LT-3/ LT-4

3 P.T.O.

(b) How are the following obtained in a laboratory ?Distilled water. 2

De-ionised water. 3(c) What is meant by temporary and permanent hardness of water ? How is 5

temporary hardness removed ?

4. Answer any five of the following : 2x5=10(a) An item of graduated glassware has the following markings, what do they mean ?

20°C10 cm3

(b) Name two substances that make insertion of a piece of glass tubing into rubbereasy.

(c) Write the common names of the following :CH3C0 CH3

Al203C6H5CH3Ca(OH)2

(d) What will be the normality of a 0.5M potassium hydroxide solution ?What is a standard solution ? Give its use.Calculate the molarity of NaC1 solution having NaC1 6.0g/200 cm3.(Mol. mass of NaC1 = 58.44 g/mol)

(g) Write the formulae of :diethyl ether andpotassium dichromate.

5. Answer the following :What is the mass of the substance when the following weights are used ?

2

g•• 5, 2, 1mg : 500, 200, 100, 50, 20rider : 5.2

Name any two types of burners used in a lab and differentiate between them. 3Draw a diagram of a Buchner funnel and highlight its use. 2

Name three types of flames and give an example of the type of work for which 3each can be used.

OREnumerate the techniques used for drying of crystals. 3

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LT-2/LT-3/LT-4 4

LT-4 : Laboratory Techniques in Physics

Time : 1 hour Maximum Marks : 50

Note : Attempt all questions.

1. Attempt any five parts : 5x3=15

In a resistance box, what is the resistance when (i) all gaps are plugged (ii) theplug from the gaps marked 5 Q, 100 Q and 1000 Q are taken out ?

A transformer has a rating 240 V/6V, 1Watt. What is the maximum current thatcan be allowed in the primary and secondary coils ?

What precautions should you take to avoid injuring yourself while working witha soldering iron ?

In a screw gauge, the main scale reading is 0.25 cm. and the circular scale reads31 divisions. What is the total reading if the least count is 0.001 cm ?

Spring balance measures weight whereas beam balance measures mass. Justifythis statement.

Suppose you are given a concave mirror and a convex mirror of focal length 30cm. How would you distinguish between them ?

(g) Calculate the value of resistance indicated by the following colour code :

Orange, Green, Violet, Gold.

2. Attempt any five parts : 7x5=35

(i) What general utilities do you expect in a physics laboratory ? List one 5

possible fault each one of them can develop.

(ii) List the apparatus kept usually in a dark room attached to the physics 2

laboratory.

(i) Explain the working of a simple barometer. 4

(ii) Using a Fahrenheit thermometer, the temperature of water was found to 3be 77°F. What will be the temperature using a Celsius thermometer ?

(c) (i) Explain how the velocity of sound in air can be obtained using resonance 3tube apparatus.

(ii) Glass is denser than water and water is denser than air. Draw the ray 4diagram when light travels from water to glass and from glass to air.

LT-2/ LT-3/ LT-4

5 P.T.O.

(d) List the precautions you should take in handling an electrolytic capacitorand a capacitor carrying high charge.

4

Draw a circuit containing a resistor R capacitor C and inductor L in serieswith a battery, B.

3

(e) What is a grating ? Define grating element. 2+2

Draw the symbols for a pn junction diode and a pnp transistor. 1+2

(f) (i) Draw a diagram showing a typical laboratory wiring system. 3

(ii) Explain the construction and working of a metre bridge. 4

(g) (i) List four main control knobs on the front panel of a CRO and state thefunction of each one of them.

4

(ii) Name two primary cells. State the difference between a primary cell and asecondary cell.

1+2

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LT-2/ LT-3/ LT-4 6

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LT-2/LT-3/LT-4 12