Low t emperature cycle (4-20 o C)
description
Transcript of Low t emperature cycle (4-20 o C)
Low temperature cycle (4-20oC) PST-127 PST-100 PST-114 PST-127 PST-127 PST-100 PST-114 PST-127
High temperature cycle (10-30oC)
AvS PI 178759 AvS PI 178759
Seedling
Adult plant
Supplementary Fig. 1 shows the reactions of PI 178759 tested with three Pst races (PST-100, PST-114 and PST-127) at seedling and adult-plant stages under low (4-20oC) and high (10-30oC) temperature cycles in comparison to AvS.
A
B
CS
PI 1
7875
9R
BSB Av
SN
1AT
1BN
1BT
1AN
1DT
1AN
2AT
2DN
2BT
2DN
2DT
2AN
3AT
3DN
3BT
3DN
3DT
3BN
4AT
4DN
4BT
4DN
4DT
4BN
5AT
5DN
5BT
5AN
5DT
5BN
6AT
6DN
6BT
6DN
6DT
6AN
7AT
7BN
7BT
7A
N7D
T7B
Supplement Fig. 2 Polyacrylamide gels showing resistance gene analog polymorphism (RGAP) markers Xwgp2132 (A) and Xwgp1315 (B) in Chinese Spring (CS), PI 178759, resistant bulk (RB), susceptible bulk (SB), AvS and the 21 nulli-tetrasomic CS lines. The arrows show the absence of both markers in line N7BT7A, indicating their locations on chromosome 7BL.