LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma...
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![Page 1: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/1.jpg)
LOGO
Dr. Shahrokh Yousefzadeh-ChabokAssociate Professor of Neurosurgery,
Guilan Road Trauma Research Center, Guilan University
Comparing GCSF and Stem Cells Obtained
From Mesenchymal Cells
in Healing of Spinal Cord Injured of Rat
![Page 2: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/2.jpg)
LOGOStudy Design
Sham
•Alone Laminec-tomy, Without SCI
Control
•Laminec-tomy
![Page 3: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/3.jpg)
LOGOStudy Design
Stem CellInteratecal Injection SCI Surgery
- - - Laminectomy Sham
- Distilled water + Laminectomy Control
- GCSF + Laminectomy GCSF
+ - + Laminectomy MSC
![Page 4: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/4.jpg)
LOGO
![Page 5: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/5.jpg)
LOGO
![Page 6: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/6.jpg)
LOGO
Within 14 injury day
Acute:
Between 14 days and 8 weeks.
Sub acute:
More than 8 weeks.
Chronic:
SCI
![Page 7: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/7.jpg)
LOGO
Muscles complications
Neuropathic Pain
Complication of BMSCT
![Page 8: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/8.jpg)
LOGO
GM- CSF (Grunulocyte macrophaye-colony stimulating factor)
G-CSF (Grunulocyte-colony stimulating factor)
Erythropoitin
Hematopoietic Cytokine
![Page 9: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/9.jpg)
LOGO
Decrease apoptosis an improvement of Neurologic
Function (Neuroprotective Effect)
GCSF Effect
![Page 10: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/10.jpg)
LOGO
Fever > 390C
Cord Transection MRI
Ventilator assisted Breathing
(ASIA) grade A, B
Exclusion Criteria of GCSF
![Page 11: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/11.jpg)
LOGO
as a growth Factor for hematopoitic progenitor cells to Treat of Nentopenia
Hematopoietic:
mobilization of peripheral blood-drieved hematopoeitic stem cells for
transplantation to treat of Neuronal Injury & Stork
Nonhematopoitic :
GCSF (Granulocyte-colony Stimulating Factor) Function
![Page 12: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/12.jpg)
LOGO
5 mg/kg/ Day for 5 days
10 mg/kg/ Day for 5 days
Dose of GCSF
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LOGO
GCSF in Acute Phase of SCI
Mobilization of bone-marrow derived cell to injury site of spinal cord
Suppresses Neuronal Apoptosis
Suppresses the death of Oligodendrocyte
Protect myelin
Suppresses the expression of cytokine such as TNF-α and IL-1B
![Page 14: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/14.jpg)
LOGO
GCSF in Subacute Phase of SCI
Neuroprotective effects via
angiogenesis after SCI
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LOGO
Exclusion Criteria
16 years old < age < 75 years old
High dose MPss (solumedrol)
Intracerebral Pathology (Tumor, Ischemia, Infections)
History of major bleeding requiring transfusion
History of leukopenia, thrombocytopenia hepatic or renal
dysfunction, sever heart failure, splenomegaly
Malignance
pregnancy
![Page 16: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/16.jpg)
LOGO
Hematopoietic Stem Cell
Mesenchymal Stem Cell
Endothelial Progenitor Cell
Macrophage
lymphocyte
BMC Sources
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LOGO
fever twice daily for 7 days
CBC once daily for 7 days
ASI Score
ASI impairment score(AIS)
Post Operative Exam
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LOGOMild Complication
Pneumonia ,Peptic Ulcer, Hepatopathy
Fever, Headache, Nausea Vomiting
Low Back Pain, Pelvic Pain
![Page 19: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/19.jpg)
LOGOSever Complication
MI
CVA
Spleen rupture
![Page 20: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/20.jpg)
LOGOMesenchymal Stem Cell Effects
Neroprotection
Neurogenesis
Neuroplasticity
![Page 21: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/21.jpg)
LOGO
Bone marrow
umbilical cord
Aniniotic fluid
Addipose tissure
Mesenchymal Stem Cell Sources
![Page 22: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/22.jpg)
LOGO
Decreased apopetotic cell death
Increase of Neurogeneration
Synaptogenesis
Remyelination
Angiogenesis
Re-formation of BBB
The Effects of MSC
![Page 23: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/23.jpg)
LOGO
In Experimental Study of MSC in Rat
Increase in axonal fiber density synaptogenesis and myelination
Secretion of trophic factor (BDNF…..)
Decrease glial scar thickness
Enhance annual growth in the penumbra
Anti inflammation by suppression of T-cell ,inhabit of B-cell and
IgG Production
![Page 24: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/24.jpg)
LOGOAdvantage of Cell Transplant
Easily localized the treatment of affected site
supply a variety of trophic factor
Persistence of long time to affect the environment injured tissue
Increased the host cell
![Page 25: LOGO Dr. Shahrokh Yousefzadeh-Chabok Associate Professor of Neurosurgery, Guilan Road Trauma Research Center, Guilan University Comparing GCSF and Stem.](https://reader036.fdocuments.in/reader036/viewer/2022062314/56649e225503460f94b0e881/html5/thumbnails/25.jpg)
LOGO